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Citations for Thermo Fisher :
2651 - 2700 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Samples were analysed using reverse phase liquid chromatography coupled to high resolution mass spectrometry (HPLC-ESI-HRMS) using a LTQ-Orbitrap XL instrument (Thermo Scientific). Samples were diluted at 1/10 in MilliQ water and 10 μL of the solution was injected into the analytical system ...
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bioRxiv - Molecular Biology 2020Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Genetics 2020Quote: ... HPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 × 250 mm, 3 μm, operated at 50°C; Thermo Fisher Scientific) connected to an HP 1100 series HPLC (Agilent ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
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bioRxiv - Cell Biology 2020Quote: ... and then separated on a reversed-phase EASY-Spray analytical column (PepMap RSLC C18, 2 μm, 75 μm i.d. × 50 cm, Thermo Fisher Scientific) using a linear gradient of 4-32% B (buffer A ...
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bioRxiv - Molecular Biology 2020Quote: ... the aqueous phase was transferred to a fresh tube with 340 μl of isopropanol and 1 μl of GlycoBlue (Invitrogen #AM9515), then precipitated at -80°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min acetonitrile gradient ...
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bioRxiv - Molecular Biology 2021Quote: ... Dried peptide mix was resuspended in 2% TFA and fractionated using Pierce High pH Reversed-Phase Fractionmation kit (84868, ThermoFisher Scientific). Each fraction was vacuum-dried and resuspended in 25 μl 0.1% TFA ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from log phase bacterial cultures grown in LB at 37°C with shaking and quantified using a Qubit 2.0 Fluorometer (Life Technologies, USA) using the dsDNA HS kit ...
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bioRxiv - Plant Biology 2021Quote: ... A Thermo Scientific EASY-nLC 1000 chromatograph was used to separate protein complexes with a high degree of resolution using a reversed-phase column (Easy-Spray PepMap RSLC C18 column-3 μm, 100 A, 75 μm × 150 mm; Thermo Scientific) at 35°C ...
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bioRxiv - Systems Biology 2020Quote: ... 100 μg purified peptides from both yeast and HeLa digest were each fractionated at pH 10 on a reversed phase column (Waters Acquity CSH C18 1.7 μm 1 × 150 mm) using a Dionex Ultimate 3000 system (Thermo Fisher Scientific). The fractions were freeze-dried and re-constituted in 0.1% formic acid.
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Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... Tryptic peptides form each of the 4 replicates were separated by the reversed phase C18 column on an EASY-nLC 1000 (Thermo Scientific) system at a flow rate of 200 nL/min using a 70 min gradient from 5% B to 45% B (A ...
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bioRxiv - Microbiology 2021Quote: ... Bacterial strains were grown at 37°C with shaking (180 rotations per minute (r.p.m.)) for 18 hours to stationary phase in Luria Broth (LB; Thermo Fisher Scientific, USA). For culture on solid media ...
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bioRxiv - Biochemistry 2020Quote: Processed peptides were measured by reversed phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an EASY nLC1000 (Thermo Scientific) coupled to a quadrupole orbitrap mass spectrometer (Q-Exactive ...
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bioRxiv - Genetics 2021Quote: ... coupled to an Easy 1000 reverse phase nano-flow LC system (Proxeon) using the Easy nano-electrospray ion source (Thermo Fisher). Five microliters of peptide mixture were loaded onto an Acclaim PepMap™ precolumn (75 μm x 2 cm ...
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bioRxiv - Genomics 2021Quote: Yeast cells were grown in YPD (yeast extract-peptone-dextrose) medium to mid-log phase and treated with 0.4% (v/v) MMS (Acros Organics, AC15689) for 10 min to induce alkylation damage ...
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bioRxiv - Microbiology 2021Quote: ... ~108 CFU of the indicated strains grown to late log phase under competence-inducing conditions were washed in IO and incubated with 50 μg/mL biotin-maleimide (biotin-mal, Thermo Fisher) for 45 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Ten different Bacteroides strains in stationary phase (1mL) were subcultured into 9 mL of liquid sABB (Anaerobe Basal Broth, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... and separated on a C18 reversed phase column (Acclaim PepMap RSLC, 75 μm x 50 cm (C18, 2 μm, 100 Å)) (ThermoFisher) using a gradient of 2% to 35% B in 180 min (Solvent A ...
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bioRxiv - Microbiology 2021Quote: ... fitted with a 100 μm x 2 cm PepMap100 C18 Nano Trap column and a 75 μm×25 cm reverse phase C18 nano column (Aclaim PepMap, Thermo Scientific). Samples were separated using a binary gradient consisting of buffer A (2% MeCN ...
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bioRxiv - Cell Biology 2021Quote: ... cryo-ET data of nucleocapsids in situ at control and ~1 h of 0.5 mM As(III) stress were also collected with a Volta phase plate58 (VPP, Thermo Fisher Scientific) for the purpose of better visualization ...
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bioRxiv - Cell Biology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Cell Biology 2021Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Fisher Scientific). In brief ...
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bioRxiv - Genomics 2021Quote: ... and analyzed by UHPLC-MS (Ultra-high performance liquid chromatography-mass spectrometry) on a reversed phase column (2.1 × 50 mm, 1.5 μm, Accucore Vanquish, Thermo Scientific, Waltham, MA) using a gradient of acetonitrile (MeCN ...
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bioRxiv - Microbiology 2022Quote: ... Separation of peptides was performed by C18 reverse-phase chromatography at a flow rate of 300 nL/min using a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min acetonitrile gradient ...
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bioRxiv - Molecular Biology 2022Quote: ... peptides were desalted on self-made reverse-phase C18 StageTips columns and analyzed by LC-MS/MS using LTQ-Orbitrap XL (Thermo Scientific) coupled with an Agilent 1200 nanoflow LC via nanoelectrospray ion source (Proxeon) ...
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bioRxiv - Cancer Biology 2022Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (ThermoFisher Scientific, Inc). In brief ...
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bioRxiv - Biochemistry 2022Quote: ... at a flow rate of 400 nL/min and then separated using a reversed-phase analysis column (50 μm×15 cm, C18, 2 μm, Thermo Fisher) for 120 minutes ...
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bioRxiv - Cell Biology 2022Quote: Peptide samples were injected and separated by an UltiMate3000 RSLCnano system (EASY-Spray C18 reversed-phase column, 75µm × 50cm, 2 µm, Thermo Fisher Scientific) using the following LC gradient ...
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bioRxiv - Developmental Biology 2022Quote: ... the aqueous phase was transferred to a fresh tube with 340 μl of isopropanol and 1 μl of GlycoBlue (Invitrogen #AM9515), then precipitated at −20°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Fisher Scientific). In brief ...
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bioRxiv - Cell Biology 2022Quote: Samples were analyzed by reversed phase nLC-MS/MS using an Ultimate 3000 UHPLC coupled to an Orbitrap Q Exactive HF-X mass spectrometer (Thermo Scientific). Digested peptides were separated over a 50 cm reversed phase column ...
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bioRxiv - Cancer Biology 2022Quote: Both the normal DIA and phospho-DIA library composite samples were fractionated into 96 fractions with a high pH reverse phase offline HPLC fractionator (VanquishTM, ThermoFisher Scientific). Mobile phase A is DI H2 O with 20 mM Formic Acetate ...
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bioRxiv - Plant Biology 2022Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano-Easy-spray column (Thermo Scientific PepMap C18 ...
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bioRxiv - Plant Biology 2022Quote: ... connected to the C18-reversed phase analytical column (EASY-Column, 10 cm long, 75 μm inner diameter, 3 μm particle size; Thermo Scientific) in buffer A (0.1% formic acid ...
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bioRxiv - Microbiology 2022Quote: ... cell pellets collected from 1 mL early exponential phase cultures were re-suspended in 500 µL TRIzol Reagents (ThermoFisher, CN 15596026) and frozen at -80 C for at least 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... nano-flow reverse phase liquid chromatography and tandem MS was performed using Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific) as described previously58 ...
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bioRxiv - Molecular Biology 2023Quote: ... employing a 75 μm x 150 mm Easy spray column packed with C-18 reverse phase (Thermo Fisher Scientific, Cat# ES901) and a trapping column (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: The phosphopeptide enrichment step flow through and washes were dried down and approximately 120 µg peptides were fractionated using the TMT fractionation protocol of Pierce high pH basic reversed-phase peptide fractionation kit (Thermo Fisher Scientific™ Cat no 84858 ...
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bioRxiv - Neuroscience 2023Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an UltiMate® 3000 liquid chromatography system (Thermo Scientific). For reversed-phase chromatography ...
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bioRxiv - Plant Biology 2023Quote: Methanolic extracts were analyzed via reversed-phase LC-MS on a Dionex UltiMate 3000 Quaternary Rapid Separation UHPLC+ focussed system (ThermoFisher Scientific) coupled to an ESI QTOF Compact mass spectrometer (Bruker ...
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bioRxiv - Biochemistry 2022Quote: Peptides were analysed using a Dionex Ultimate 3000 nano-ultra high pressure reversed-phase chromatography system coupled on-line to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific). Samples were separated on an EASY-Spray PepMap RSLC C18 column (500 mm x 75 μm ...
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bioRxiv - Neuroscience 2023Quote: ... Eluted peptides were lyophilized and reconstituted in 300 µL 0.2% TFA for fractionation using the Pierce High pH Reversed-Phase Peptide Fractionation Kit following the instructions provided by Thermo Fisher Scientific for TMT-labelled samples ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Molecular Biology 2022Quote: Peptides were analysed by reversed-phase liquid chromatography on an EASY-nLC 1000 or 1200 system (Thermo Fisher Scientific, Odense, Denmark) coupled to a Q Exactive plus or HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... 800 µL of the bottom phase was transferred to scintillation vials containing 5 mL ScintiVerse BD Cocktail (Fisher Scientific SX18-4). The vials were vortexed and radioactivity was measured in a Scintillation Counter (Tri-Carb 2819TR ...
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bioRxiv - Cell Biology 2022Quote: ... The purified desalted sample was pre-fractionated into 40 primary fractions with basic reversed-phase chromatography (bRP-LC) using a Dionex Ultimate 3000 UPLC system (Thermo Scientific). Peptide separations were performed using a reversed-phase XBridge BEH C18 column (3.5 μm ...
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bioRxiv - Cancer Biology 2022Quote: ... the polar metabolite phase was resuspended in 50 μL water and analyzed in a Dionex UltiMate 3000 LC System (Thermo Scientific) with a thermal autosampler set at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Peptides were separated on reversed phase C18 columns (trapping column: Acclaim PepMap 100, 300 μm × 5 mm, 5μm, 100 Å, Thermo Fisher Scientific ...