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Citations for Thermo Fisher :
2501 - 2550 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was transferred to a new tube and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84688) following manufacturer’s recommendation.
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bioRxiv - Cell Biology 2023Quote: ... samples were analysed by reverse-phase chromatography using an UltiMate 3000 UHPLC connected to an Orbitrap Fusion Lumos (Thermo Fisher). Peptides were trapped on a PepMap C18 trap column (300 µm x 5 mm ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% FA and analyzed by Reversed Phase (RP) LC-MS/MS using an EASY-nLCTM 1200 system (Thermo Fisher Scientific) coupled to an Orbitrap LumosTribridTM mass spectrometer equipped with FAIMS ProTM Interface (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were separated on a reversed phase column (nano viper Acclaim PepMap capillary column, C18; 2 µm; 75 µm × 50 cm, ThermoFisher) at a flow rate of 200 nL/min increasing organic acetonitrile phase for 60 min as described before ...
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bioRxiv - Cell Biology 2023Quote: ... The adipogenesis quantification at the macro-scale X40 for mapping the level of adipogenesis (LOA) follow up using phase-contrast EVOS FL Auto-2 Microscope (Invitrogen) (60) ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: BM-MSCs in logarithmic growth phase and in good condition were washed twice with PBS (Gibco, Grand Island, NY, USA). Subsequently ...
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bioRxiv - Microbiology 2024Quote: LC-MS/MS analysis was performed using an UltiMate® 3000 nano splitless reversed-phase nanoHPLC (Thermo Fisher Scientific, Dreieich) coupled online to a timsTOF™ pro mass spectrometer (Bruker Daltonik GmbH ...
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bioRxiv - Immunology 2022Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated into eight fractions using the High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific Inc.). The fractions were dissolved in 0.1% formic acid ...
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bioRxiv - Biochemistry 2022Quote: ... subjected to reverse phase clean-up step and analyzed by LC-MS/MS on a Q Exactive Plus (Thermo Scientific) as previously described (44) ...
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bioRxiv - Plant Biology 2022Quote: ... The peptides were loaded onto a reversed phase trap column (Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Scientific) connected to the C18-reversed phase analytical column (EASY-Column ...
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bioRxiv - Genomics 2022Quote: ... Extractions were centrifuged for 10 min at 17,900 x g and the upper aqueous phase was transferred to new microfuge tubes and the DNA precipitated by adding 0.5 μl GlycoBlue Coprecipitant (Invitrogen AM9515), 0.1 volumes of 3M sodium acetate ...
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bioRxiv - Genomics 2022Quote: ... Extractions were centrifuged for 10 min at 17,900 x g and the upper aqueous phase was transferred to new microfuge tubes and the DNA precipitated by adding 0.5 μl GlycoBlue Coprecipitant (Invitrogen AM9515), 0.1 volumes of 3M sodium acetate ...
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bioRxiv - Immunology 2022Quote: ... Peptides were separated by reverse phase chromatography using a C18 pre-column (Acclaim PepMap 100, 75μm×2cm, Thermo Fisher Scientific) followed by C18 reversed-phase LC column (PepMap RSLC C18 ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was extracted by phase separation and poly(A) selection was performed using Dynabeads mRNA DIRECT Purification Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The cultures were then grown for ∼3 hours and total RNA was isolated from mid-log phase cultures using TRIzol Max Bacterial Isolation kit (ThermoFisher). RNA was quantified using a Qubit 4.0 fluorometer ...
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bioRxiv - Microbiology 2022Quote: ... Labelled NulOs were analyzed by reverse phase chromatography Orbitrap mass spectrometry (QE plus quadrupole Orbitrap, Thermo Fisher Scientific, Waltham, MA). NulOs were identified according to their mass ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were separated on a 250 mm x 75 μm Acclaim PepMap® C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Images of U87vIII and mtDsRed-expressing U87 spheroids were acquired by phase contrast and RFP fluorescence channel using a 4x/0.13 objective with an EVOS FL® Cell Imaging System (Invitrogen) and images of mtDsRed-expressing U87 and GL261 spheroids were acquired by RFP fluorescence channel using a 4x/0.1 objective with a Leica DM IL LED® (Leica ...
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bioRxiv - Microbiology 2023Quote: Peptides for proteome analyses were separated by reverse phase liquid chromatography (LC) on an RSLCnano system (Ultimate 3000, Thermo Scientific) coupled online to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then separated into 8 fractions using the High pH reversed-phase peptide Fractionation Kit (ThermoFisher Scientific, cat# 84868). The fractions were dissolved with 0.1% formic acid and peptide concentration was determined with Quantitative Colorimetric Peptide Assay (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... packed with Halo C18 reversed-phase resin (2.7 µm particle size, 90 nm pore size, MichromBioresources.) was used with EASY-nLC 1200 system (Thermo Fisher). The nanospray ESI was fitted onto the Thermo Q-Exactive plus mass spectrometer (Thermo Electron ...
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bioRxiv - Cancer Biology 2023Quote: ... Percentage of RPA-positive or γH2AX-positive cells in S-phase (EdU-positive) was analyzed by Attune NxT flow cytometer (Thermofisher) and results were quantified by FCS Express (De Novo software).
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bioRxiv - Immunology 2022Quote: ... 1μg was analysed for each fraction using a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) coupled to an Orbitrap Q Exactive Plus mass spectrometer (Thermo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Fisher Scientific, Waltham, MA) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The mixture of TMT-labeled peptides was acidified and fractionated using a high pH reversed-phase peptide fractionation kit (ThermoFisher) according to the manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2023Quote: ... 75 μm inner diameter C18-reversed phase column packed with 3 μm resin (EASY-Column Capillary Columns, Thermo Fisher Scientific) in buffer A (2% [v/v] acetonitrile and 0.1% [v/v] formic acid ...
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bioRxiv - Physiology 2023Quote: ... the labeled sample was separated into five fractions using the high pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific). Bound peptides were eluted with 1 ...
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bioRxiv - Biochemistry 2023Quote: We acidified the digested samples with concentrated trifluoroacetic acid (TFA) and cleaned up the peptides with C18 SOLA Solid-Phase Extraction (SPE) columns (ThermoFisher). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 300 μl 0,1% TFA and fractionated on High pH reserved-phase fractionation spin-column (Thermo Scientific) according to the manufacturer’s instructions for TMT-labelled peptide samples ...
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bioRxiv - Microbiology 2024Quote: ... coupled to an Easy 1,000 reverse phase nano-flow LC system (Proxeon) using the Easy nano-electrospray ion source (Thermo Fisher). Peptide mixtures were analyzed in triplicate ...
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bioRxiv - Molecular Biology 2024Quote: ... and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID, Acclaim PepMap 100, C18, 2 µm) (Thermo Scientific). Chromatography solvents were (A ...
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bioRxiv - Microbiology 2024Quote: ... with a 30 cm C18-reverse phase column coupled on-line to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific). Data was acquired in data-dependent top speed mode.
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated on a C18 reversed phase column (PepMap100 C18, 25 cm × 75 μm i.d., 3 μm; Thermo Scientific) at a flow rate of 250 nl/min ...
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bioRxiv - Microbiology 2024Quote: Logarithmic phase trophozoites were used to extract the total amoebic RNA using PureLink™ RNA Mini Kit (12183020, Invitrogen, USA) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The total protein was extracted from log-phase cells (OD660 = 1.0) with a NuPAGE LDS sample buffer (Thermo Fisher Scientific) after 0.2 mol/l NaOH treatment (Kushnirov 2000) ...
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bioRxiv - Physiology 2024Quote: ... The average percentage of CD42b covered area was quantified from five different fields per channel on a fluorescence phase-contrast microscope (EVOS M5000; Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... was pooled into one sample and desalted using a SOLAµ Solid Phase Extraction (SPE) column (ThermoFisher Scientific, Cat. 60209-001) following the standard cleanup protocol ...
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bioRxiv - Physiology 2024Quote: ... and 520 µL of the upper (organic) phase was transferred to a lipidomics collection vial (National Scientific #CPSH0008, Thermo Scientific). The second extraction was performed via addition of 600 µL of MTBE to the source vial ...
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bioRxiv - Neuroscience 2024Quote: ... spheroids were lifted and returned to suspension culture in Phase III media: 1X Advanced DMEM/F12 (Thermo Fisher Scientific, 12491015), 0.5X N2 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptide separation was performed using a reversed-phase analytical column (Easy-spray PepMap RSLC, 0.075 × 250 mm, Thermo Fisher Scientific) with a linear gradient of 4%-27.5% solvent B (0.1% formic acid in 80% acetonitrile ...
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bioRxiv - Microbiology 2024Quote: ... The plates were covered by the lid with pegs (Nunc – Transferable Solid Phase screening system (Nunc-TSP, Thermo Fisher Scientific), which submerged into the bacterial cultures in the wells and were incubated overnight at 37°C to form biofilms on the Nunc-TSPs of the lid.
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bioRxiv - Microbiology 2024Quote: ... The plates were covered by the lid with pegs (Nunc – Transferable Solid Phase screening system (Nunc-TSP, Thermo Fisher Scientific), which submerged into the bacterial cultures in the wells and were incubated overnight at 37°C to form biofilms on the Nunc-TSPs of the lid.
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bioRxiv - Microbiology 2024Quote: ... Nanoindentation measurements were performed using a Chiaro Nanoindenter (Optics 11) mounted on top of an inverted phase contrast microscope (Evos XL Core, Thermofisher), following a previously described approach.[41] Measurements were performed at room temperature in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... UHPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 x 150 mm, 2.6 μm operated at 50°C; Thermo Fisher Scientific) connected to Vanquish Horizon UHPLC system and an Orbitrap Exploris 240 MS (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... in mobile phase A and resolved over a 120-minute gradient of mobile phase B (0.1% Formic acid, 80% acetonitrile) using an EASY-Spray column (ES803A, Thermo Scientific) maintained at 45° C ...
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bioRxiv - Cancer Biology 2024Quote: ... ARID1A protein expression quantified by reverse phase protein array of 127 patients and RNA-seq (HTSeq counts; Affymetrix SNP6 arrays) data available for 410 tumors were analyzed ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatography 1 used a 30-minute reversed-phase chromatography Accucore C30 column (2.6 μm, 2.1 mm x 150 mm, 27826-152130, Thermo Fisher Scientific) combined with an Accucore C30 guard column (2.6 μm ...
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bioRxiv - Cell Biology 2024Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...