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Citations for Thermo Fisher :
2401 - 2450 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... using the Gateway BP Clonase II Enzyme mix (Invitrogen 11789020). Expression vectors were generated by cloning into pInducer20 (gift from Stephen Elledge ...
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bioRxiv - Cancer Biology 2024Quote: ... The first strand cDNA was synthesized using Superscript II (Invitrogen) and random hexamers ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by mounting on Lab-Tek II Chambered Coverglass (Nunc) with ProLong Diamond Antifade Mountant (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... and Gateway LR Clonase II enzyme mix (11791, Invitrogen, Canada). After transformation into DH5alpha E.coli competent cells ...
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bioRxiv - Microbiology 2024Quote: ... eight-well chambers Lab-Tek II (Thermo Scientific, cat#155409) at 37°C in 5% CO2 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using BP Clonase II Enzyme mix (11789100, ThermoFisher, Waltham, MA). The recombination reactions were used to transform DH5alpha competent cells (C2987U ...
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bioRxiv - Developmental Biology 2024Quote: ... and cDNA synthesis was performed by using SuperScript II (Invitrogen). qRT-PCR was performed with Power SYBR Green PCR Master Mix by the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems ...
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bioRxiv - Biochemistry 2024Quote: ... stained with 1x SYBR Green II RNA Gel Stain (Invitrogen) in 1X TBE for 10 mins ...
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bioRxiv - Synthetic Biology 2024Quote: ... using the Gateway LR Clonase II Enzyme mix (Life Technologies). Resultant plasmids were co-transformed into CEN.PK2-1D using the frozen-EZ yeast transformation II kits (Zymo Research) ...
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bioRxiv - Biochemistry 2024Quote: ... through an LR Clonase II reaction (Life Technologies, Carlsbad, CA). pcDNA constructs were also sequenced additionally prior to lipofection ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was synthesized using the Superscript II Reverse Transcriptase (Invitrogen) and quantitative PCR were paerformed with ONEGreen FAST qPCR Premix provide by Ozyme using 10µM of each specific primer on a 7500 Fast RealPCR system (Applied Biosystems) ...
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bioRxiv - Biophysics 2024Quote: ... Sf21 cells cultured in SFM Sf-900 II medium (Gibco), at a density of 4 × 106 cells/ml ...
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bioRxiv - Cell Biology 2024Quote: ... using TaqMan Universal Master Mix II with UNG (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... containing 5% Protein Free Hybridoma Medium (PFHM II; ThermoFisher Scientific). On day 0 ...
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bioRxiv - Cancer Biology 2024Quote: ... digested in DMEM containing 3 mg/mL Dispase II (Gibco) and 1 mg/mL Collagenase IV (C5138;Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... Reverse transcription was performed using Superscript II reverse transcriptase (Invitrogen) or MarathonRT (Kerafast ...
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bioRxiv - Cancer Biology 2024Quote: ... and counted using a Countess II Automated Cell Counter (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... The cell concentration was verified using a Countess II (Invitrogen). All submissions had a cell concentration near 1,000 cells/μL and cell viability above 70% except one with 51%.
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bioRxiv - Developmental Biology 2024Quote: ... Collagenase II (255 units/mg, 17101-015, Life Technologies, USA) and Collagenase IV (310 units/mg ...
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bioRxiv - Cell Biology 2024Quote: ... using TaqMan Universal Master Mix II with UNG (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... then concatenated into 12 by high-pH C18 reversed phase chromatography before analysis on an Orbitrap Fusion (Thermo Fisher Scientific) over the course of 2 h over 2–30% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... Eluted DNA was purified with solid-phase reversible immobilization (SPRI) purification with Sera-Mag carboxylate-modified Magnetic SpeedBeads (Fisher Scientific) at a 1.8:1 ratio ...
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bioRxiv - Developmental Biology 2020Quote: Cell cycle phase distribution analysis was conducted using Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Life Technologies) and FxCycle Violet (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... A Neuropixels probe (option 3 phase A) with 374 recording sites was briefly (∼2 minutes) dipped into the diI cell-labeling solution (ThermoFisher) to visualize probe tracks ...
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bioRxiv - Molecular Biology 2021Quote: ... The aqueous phase with RNA was transferred into a tube and combined with 10 μg of Linear Acrylamide (Ambion, AM9520), 1/10 of sample volume of 3M CH3COONa (pH 5.2) ...
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bioRxiv - Immunology 2022Quote: ... Samples (3 μg) were injected onto a MAbPac reversed-phase liquid chromatography column (Thermo Fisher Scientific, 2.1 mm × 50 mm) heated to 80°C using a Dionex Ultimate 3000 RSLC system ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The combined filtrates were desalted on a high pH reversed-phase peptide fractionation column (Pierce™, Thermo Fisher Scientific, USA). Then the peptides were collected followed by vacuum drying ...
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bioRxiv - Pathology 2020Quote: ... Reverse-phase mobile buffers were composed of A: 0.1% (v/v) FA (Thermo Scientific, MA, US, Cat No. 34851-4), and B ...
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bioRxiv - Biochemistry 2021Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...
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bioRxiv - Biochemistry 2020Quote: ... Enriched glycopeptides were analyzed by reverse-phase liquid chromatography coupled online to RP-LC-ESI-OT MS/MS (ThermoFisher Scientific) using two higher-energy collision dissociation fragmentation regimes (HCD.low and HCD.step) ...
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bioRxiv - Plant Biology 2021Quote: ... The DNA in aqueous phase was precipitated with sodium acetate and ethanol along with 1 μl glycogen carrier (Thermo Scientific) overnight at −20°C and then centrifuged to pellet at 4°C (12,000 g ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse phase chromatography was performed at 50°C with an EASY-nLC 1000 ultra-high-pressure system (Thermo Fisher Scientific) coupled to the Q Exactive mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... were performed using the EZ-link NHS-PEO Solid Phase Biotinylation Kit (Pierce) according to the manufacturer’s protocol and purified using MINI Dialysis Unit (ThermoFisher, 69576). 500 ng/mL biotinylated mAbs were added to each well ...
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bioRxiv - Cell Biology 2021Quote: The digested peptides were analyzed by reverse phase LC-MS/MS on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) coupled to a Dionex UltiMate 3000 -nLC (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Phase contrast imaging and fluorescence microscopy was carried out following standard procedures (EVOS FL Cell Imaging System, Thermo Fisher Scientific), routinely at least 48 h post-transfection or post-transduction of target cells with fluorophore expressing constructs.
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bioRxiv - Microbiology 2022Quote: Peptides were fractionated into eight fractions using the high pH reversed-phase peptide fractionation kit (Pierce™ Thermo Fisher Scientific) according to the manufacturer’s instructions and using self-made columns as previously described48 ...
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bioRxiv - Cell Biology 2022Quote: ... and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID, Acclaim PepMap 100, C18, 2 μm) (Thermo Scientific). Chromatography solvents were (A ...
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bioRxiv - Biochemistry 2021Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...
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bioRxiv - Microbiology 2021Quote: Combined TiO2 and Fe-NTA phosphopeptide eluates were fractionated using the Pierce High pH Reversed-Phase kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The Nano-LC mobile phase was introduced into the mass spectrometer using an EASY-Spray™ Source (Thermo Scientific™). During peptide elution ...
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bioRxiv - Biochemistry 2021Quote: ... of the sample were desalted and concentrated by injection on a reversed-phase cartridge (MSPac DS-10, 2.1×10 mm, Thermo Scientific) at 60°C using buffer A (0.1 % formic acid ...
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bioRxiv - Cell Biology 2022Quote: ... reversed phase HPLC column (25 cm length × 75 µm inner diameter) directly injected to an Orbitrap Eclipse using a 120 min gradient (mobile phase A = 0.1% formic acid (Thermo Fisher), mobile phase B = 99.9% acetonitrile with 0.1% formic acid (Thermo Fisher) ...
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bioRxiv - Genetics 2022Quote: ... The cells were collected at exponential phase by centrifugation and resuspended in pre-warmed staining solution that consist of MitoTracker Red CMXRos (Invitrogen) at 200 nM ...
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bioRxiv - Microbiology 2022Quote: ... and the aqueous layer was transferred to a 2 ml phase lock gel tube (QuantaBio) alongside an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1) solution (Invitrogen; 15593-031). Samples were centrifuged (18,000 x g for 5 min at 4°C) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were cultured in DMEM with 10% FBS and 1% P/S and were bulked up to 9 x T175 flasks of each by splitting and reseeding during their growth phase (∼80% confluence) using TrypLE (Gibco). The 9 x T175 flasks were seeded at ∼30% confluence in serum-containing media ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5ml of overnight culture in SC-URA 2% Galactose was pelleted and RT-DNA was isolated by RNAse A/T1 treatment of the aqueous (RNA) phase after TRIzol extraction (Invitrogen), following the manufacturer’s recommendations with few modifications ...
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bioRxiv - Neuroscience 2021Quote: ... tau proteins were measured with Tau-5 antibodies using ELISA according to manufacturer’s instructions (The Human Tau Solid-phase sandwich ELISA, #KHB0041, Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted using phenol/chlorophorm/isoamylalcohol phase-separation followed by a GeneJET RNA Purification Kit (Thermo Scientific™), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...