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Citations for Thermo Fisher :
2701 - 2750 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... the samples were enriched using solid-phase micro-extraction (SOLAμ HRP 10 mg/1 mL 96 well plate, ThermoFisher Scientific, Germany) and reconstituted in 100 μL 5% methanol containing an instrument control standard (ICS ...
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bioRxiv - Neuroscience 2024Quote: ... The aqueous phase containing the RNA was transferred to a new tube and 5µg of RNase-free Glycogen (Thermo Fisher Scientific) have been added ...
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bioRxiv - Molecular Biology 2024Quote: ... that were spiked with 18 pmol shTMTpro-labeled AS192 triggers were fractionated using the Pierce high pH reversed phase peptide fractionation kit (Thermo Scientific). A total of eight fractions were generated ...
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bioRxiv - Plant Biology 2024Quote: ... Labeled peptides were separated into 6 fractions with Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermofisher, Cat. no. 84868) and dried in a Speed Vac at 45 °C for 3.5 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
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bioRxiv - Cell Biology 2024Quote: ... The two supernatants were combined and subjected to high pH fractionation using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific), according to the manufacturer’s instructions.
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bioRxiv - Systems Biology 2024Quote: LC-MS/MS analysis was performed using a Dionex Ultimate 3000 nano-ultra high pressure reversed-phase chromatography system coupled on-line to a Q Exactive High Field (HF) mass spectrometer (Thermo Scientific). Samples were separated on an EASY-Spray PepMap RSLC C18 column (500 mm x 75 µm ...
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bioRxiv - Microbiology 2024Quote: A volume of 6 µl phosphopeptides or 500 ng of peptides were directly loaded onto reversed-phase pre-column (Acclaim PepMap 100, Thermo Scientific) and eluted in backflush mode ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were analyzed by reversed-phase nLC-MS/MS using an Ultimate 3000 UHPLC coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific). Digested peptides were separated using a 50 cm reversed-phase column packed in-house (Agilent Poroshell EC-C18 ...
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bioRxiv - Molecular Biology 2024Quote: ... The peptide cations eluted were converted to gas-phase ions via electrospray ionization and analyzed on a Thermo Orbitrap Fusion instrument (Q-OT-qIT – Thermo Fisher). Full MS spectra were acquired in the Orbitrap instrument with a mass range of 350 to 1,400 m/z ...
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bioRxiv - Molecular Biology 2024Quote: ... Eluting peptide cations were converted to gas-phase ions by electrospray ionization and analyzed on a Thermo Orbitrap Fusion (Q-OT-qIT, Thermo Scientific). Survey scans of peptide precursors from 350 to 1400 m/z were performed in orbitrap at 120K resolution (at 200 m/z ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µl of the upper aqueous phase was transferred to a new tube and 2 µl glycogen (GlycoBlue Coprecipitant, ThermoFisher, AM9515) and 500 µl isopropanol added ...
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bioRxiv - Microbiology 2024Quote: ... A minimum of 200uL organic phase volume per sample was loaded in the Trace 1300 gas chromatogram (Thermo Fisher Scientific, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated on a capillary column (0.075 mm × 500 mm, Acclaim Pepmap 100, reverse phase C18, NanoViper, Thermo Fisher Scientific) following a gradient of 2-40% buffer B in 128 min (A = 0.1 % formic acid B = 0.1% formic acid ...
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bioRxiv - Microbiology 2024Quote: ... and separated over a 15 min reversed-phase gradient using a Vanquish HPLC interfaced directly to a Q Exactive Plus mass spectrometer (Thermo Scientific) (21) ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.5 mg of peptides were separated on a reverse phase C18 column and analyzed on QExactive HF Mass Spectrometer (Thermo-Fisher Scientific) as described previously 40 ...
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bioRxiv - Cell Biology 2024Quote: ... (Roberta Noberini et al., 2021) Peptide mixtures were separated by reversed-phase chromatography on an EASY-Spray column (Thermo Fisher Scientific), 25-cm long (inner diameter 75 µm ...
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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bioRxiv - Immunology 2024Quote: ... by a 0.6× solid phase reversible immobilization (SPRI) followed by a 0.8× SPRI and quantified using Qubit hsDNA Assay (Thermo Fisher Scientific Q32854). The quality of whole transcriptome amplification (WTA ...
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bioRxiv - Biochemistry 2023Quote: ... The leftover PFO with small amounts of aqueous phase on top was extracted once with a 100 µL solution of UltraPure Salmon Sperm DNA solution (Thermo Fisher), diluted 2500x in nuclease-free water ...
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bioRxiv - Biochemistry 2023Quote: ... at a flow rate of 300 nL min−1 over 84 min using an Acclaim PepMap nanoViper C18 reverse-phase column in an EASY-nLC system (Thermo Scientific). Fractions were transferred to a Q-Exactive Plus Orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biophysics 2024Quote: ... red fluorescent images of the uppermost layer of embedded beads were captured along with phase-contrast images of the selected regions every 30 minutes using an automated fluorescent microscope (20× objective, EVOS m7000, ThermoFisher Scientific) for a total time of 48 hours ...
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bioRxiv - Biophysics 2024Quote: ... and desalted onto a trap column (1 mM inner diameter x 2 cm, IDEx C-128) manually packed with POROS R2 reversed-phase resin (Thermo Scientific). Peptides were then separated onto a C18 analytical column (Waters Acquity UPLC BEH ...
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bioRxiv - Biochemistry 2024Quote: ... The tryptic digest was separated through online reversed-phase chromatography using a peptide trap column Acclaim PepMap 100 C18 (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The peptide cations eluted were converted to gas-phase ions via electrospray ionization and analyzed on a Thermo Orbitrap Fusion (Q-OT-qIT, Thermo Fisher). Full MS spectra were acquired in the Orbitrap with a mass range of 350-1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the labeled peptides of each group were mixed in equal amounts and fractionated by a high pH reversed-phase peptide fractionation kit (Thermo Scientific). The column was first balanced by acetonitrile and 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Microbiology 2023Quote: ... coupled to an Easy 1000 reverse phase nano-flow LC system (Proxeon) using the Easy nano-electrospray ion source (Thermo Fisher). Each peptide mixture was analyzed in duplicate ...
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bioRxiv - Microbiology 2023Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Fisher Scientific). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... The protein concentration in the dilute phase was then determined by carefully transferring 2 μL of supernatant (i.e. the dilute phase) to a NanoDrop™ Lite spectrophotometer (Thermo Scientific) and measuring the absorbance at 280nm.
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bioRxiv - Neuroscience 2023Quote: ... Separation of peptides was performed by reverse-phase chromatography at a flow rate of 300 nL/min and a Thermo Scientific reverse-phase nano Easy-spray column (Thermo Scientific PepMap C18 ...
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bioRxiv - Microbiology 2023Quote: ... Multiplexed TMT samples were separated into four fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). TMT labelled fractions were analysed in an Orbitrap Fusion Lumos using a resolution of 50,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Brightfield microscopy images were obtained right before passaging using an inverted phase-contrast microscope (10X and 20X magnification, Thermo Fisher Scientific). The chronic Dex exposure experiments were performed once in each cell line ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then pooled and dried to near completion under reduced pressure before resuspension in 1% formic acid and fractionation using a Pierce High pH Reversed Phase Peptide Fractionation Kit (ThermoFisher Scientific) with modified elution of 12 sequential fractions (10% ...
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bioRxiv - Cell Biology 2023Quote: The HPLC run was performed using a C30 reverse-phase column (Thermo Acclaim C30, 2.1 250 mm, 3 mm, operated at 40°C; Thermo Fisher Scientific) connected to a Shimadzu LC-20AD HPLC system ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was acidified with formic acid (5% final concentration) and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84868) according to Vinogradova and coworkers (Cell ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 µL (90 µg) of reconstituted mixture of labeled peptides was fractionated using Pierce High pH Reversed-Phase Peptide Fractionation kit (84868, Thermo Fisher) according to the manufacturer’s protocol for TMT-labeled peptides ...
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bioRxiv - Immunology 2023Quote: The quenched samples were then mixed and fractionated with high pH reverse-phase C18 chromatography using the Ultimate 3000 high-pressure liquid chromatography system (Thermo Scientific) at a flow rate of 500 µl/min using two buffers ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg of peptide samples were loaded onto a C18 reversed□phase trap column (100 μm × 2 cm, Thermo Fisher Scientific) equilibrated with 100% A phase ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting phosphopeptides and whole cell lysates (flow through) were fractionated using Pierce High pH Reverse-Phase Peptide Fractionation kit (Thermo Fisher). Samples were dried and reconstituted in 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Microbiology 2023Quote: The nano-RPLC (reverse phase-high performance liquid chromatography, RP-HPLC) separation was performed on EASY-nLC1000 system (Thermo Fisher Scientific). Mobile phase A and B were 0.1% formic acid in water and 0.1% formic acid in acetonitrile ...
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bioRxiv - Cell Biology 2023Quote: ... was injected on a capillary reversed-phase column (C18 Acclaim PepMap100 A, 75 µm i.d., 50-cm length; Thermo Fisher Scientific) at a flow rate of 220 nL/min ...
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bioRxiv - Cell Biology 2023Quote: ... UHPLC analysis was performed employing a C30 reverse-phase column (Thermo Acclaim C30, 2.1x 250 mm, 3 μM, operated at 55° C; Thermo Fisher Scientific) connected to a Dionex UltiMate 3000 UHPLC system and a Q-Exactive Orbitrap high resolution mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were loaded onto a 50 cm reversed-phase column (75 mm inner diameter; Acclaim PepMap 100 C18; Thermo Fisher Scientific) and separated with an UltiMate 3000 RSLC system (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and the 1st day of stationary phase (day 5) (growth analysis data not shown) and re-suspended with Trizol solution (Ambion, USA) for RNA extraction to obtain total RNA ...
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bioRxiv - Physiology 2023Quote: ... UHPLC analysis was performed on a C30 reverse-phase column (Thermo Acclaim C30, 2.1 x 250 mm, 3 μm operated at 55° C; Thermo Fisher Scientific) connected to a Dionex UltiMate 3000 HPLC system and a QExactive orbitrap MS (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were trapped to a reverse phase trap column (Acclaim PepMap, C18 5 µm, 100 µm x 2 cm, Thermo Scientific) connected to an analytical column (Acclaim PepMap 100 ...
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bioRxiv - Neuroscience 2023Quote: LC-MS/MS analysis was performed using a Dionex Ultimate 3000 nano-ultra high-pressure reverse-phase chromatography coupled on-line to a Q Exactive Orbitrap mass spectrometer (Thermo Scientific). Prior to introduction to the MS ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a nano-HPLC (Ultimate 3000, Dionex) with an inverse phase C18 Pepmap® column (Dionex, Thermo Fisher), following a gradient of 100% solution A (0.1% formic acid ...
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bioRxiv - Cell Biology 2022Quote: ... The flow through fraction was then fractionated into 13 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher).
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bioRxiv - Biochemistry 2023Quote: ... from BN-PAGE gel lanes were analyzed by LC/MS/MS using nanoscale reverse-phase HPLC and an LTQ Orbitrap XL mass spectrometer (Thermo Scientific). Proteins were identified with the MASCOT algorithm (v2.4 ...