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Citations for Thermo Fisher :
2451 - 2500 of 7029 citations for Ammonium Polyphosphate phase II 02 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... all proteolytic digests were acidified by addition of 10% formic acid to pH 2 and directly analyzed by LC-ESI-MS(/MS) using an a capillary BioBasic C18 reversed-phase column (BioBasic-18, 150 x 0.32 mm, 5 μm, Thermo Scientific), installed in an Ultimate U3000 HPLC system (Dionex) ...
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bioRxiv - Cancer Biology 2021Quote: Two-dimensional fractionation—Peptides were fractionated using a Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific, 84868) according to the manufacturer’s instructions with slight modifications ...
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bioRxiv - Neuroscience 2020Quote: ... Phase-contrast images of 2-month-old 3D organoids were acquired on an EVOS cell imaging system (Thermo-Fisher Scientific) for size measurements ...
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bioRxiv - Cell Biology 2020Quote: ... In all cases peptides were separated using reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with a C18 trap to Acclaim C18 PepMap RSLC column (Dionex ...
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bioRxiv - Cell Biology 2020Quote: ... The final concentration of the TMT labeled digest/peptide mixture was 0.33 μg/μl and 5 μl of this mixture was loaded onto a C18-reversed phase column (Thermo Scientific, Acclaim PepMap C18 column ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2 μL of each fraction was resolved on a nano-capillary reverse phase column (PepMap RSLC C18 column, 75 μm i.d. × 50 cm; Thermo Scientific) at the flow rate of 300 nL/min using 0.1% formic acid/acetonitrile gradient system (2-22% acetonitrile in 110 min;22-40% acetonitrile in 25 min ...
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bioRxiv - Microbiology 2021Quote: ... was separated over a 30 cm C18-reverse phase column (1.8 μm Reprosil-Pur C18-AQ, dr. Maisch) and eluted using an Easy-nLC 1000 (Thermo Scientific) over a 94 min gradient (5.6% acetonitrile/0.1% formic acid - 25.6% acetonitrile/0.1% formic acid) ...
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bioRxiv - Cell Biology 2021Quote: ... Five μL aliquots of the digests were loaded onto a 75 μm Acclaim Pepmap C18 reverse phase column (Thermo Scientific) and eluted by an acetonitrile/0.1% formic acid gradient ...
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bioRxiv - Cancer Biology 2020Quote: ... packed in house with reverse phase Reprosil Pur Basic 1.9μm (Dr Maisch GmbH) using an EASY-nLC 1200 (Thermo Fisher Scientific) coupled online to an Orbitrap Q-Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: RNA was isolated from the aqueous phase of homogenized spleens mixed with 1-bromo-3-chloropropane and purified with the PureLink RNA kit (Invitrogen) separately ...
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bioRxiv - Cancer Biology 2020Quote: ... The separation prior to MS used online reversed-phase chromatography realized with a Proxeon Easy-nLC-1000 system (Thermo Scientific) equipped with an Acclaim PepMap trap column (75 μm ID x 2 cm ...
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bioRxiv - Biophysics 2021Quote: ... Tilt series were either collected with a 70 μm objective aperture or a Volta phase plate (VPP, Thermo Fisher Scientific) with prior conditioning for 6 min.
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bioRxiv - Cell Biology 2021Quote: ... and unsorted TK6 cell digests were fractionated by high-pH reverse phase chromatography using an Ultimate 3000 HPLC (ThermoFisher Scientific) and a 1 x 100 mm 1.7 µm Acquity UPLC BEH C18 column (Waters) ...
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bioRxiv - Cancer Biology 2021Quote: ... Two microliters from each fraction were resolved in 2D on a nanocapillary reverse phase column (Acclaim PepMap C18, 2 micron particle size, 75 μm diameter × 50 cm length, ThermoFisher) using a 0.1% formic/acetonitrile gradient at 300 nL/min (2–22% acetonitrile in 150 min ...
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bioRxiv - Microbiology 2021Quote: ... total RNA was extracted by phase separation and poly(A) selection was performed using Dynabeads mRNA DIRECT Purification Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were regularly monitored for bacterial contamination by checking for bacterial growth in Zobell marine broth 2216 (HiMedia) (71) in addition to filtering 2-3 mL of exponential-phase growing culture and using Sybr Green I (Invitrogen) staining and epifluorescence microscopy (Nikon Eclipse 80i ...
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bioRxiv - Immunology 2021Quote: ... was performed using the EZ-link NHS-PEO Solid Phase Biotinylation Kit (Pierce) according to the manufacturer’s protocol and purified using MINI Dialysis Unit (ThermoFisher, 69576). 500 ng/mL biotinylated mAbs were added to each well ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of peptides was injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) and electrosprayed into an Orbitrap mass spectrometer (LTQ Orbitrap Velos Pro ...
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bioRxiv - Biochemistry 2020Quote: The dried aqueous phase was measured using ultra-performance liquid chromatography coupled to an Exactive mass spectrometer (Thermo Fisher Scientific) in positive and negative ionization modes ...
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bioRxiv - Biochemistry 2020Quote: ... the dried organic phase was measured using ultra-performance liquid chromatography coupled to an Exactive mass spectrometer (Thermo Fisher Scientific) in positive and negative ionization modes ...
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bioRxiv - Immunology 2022Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated into eight fractions using the High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific Inc.). The fractions were dissolved in 0.1% formic acid ...
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bioRxiv - Biochemistry 2022Quote: ... subjected to reverse phase clean-up step and analyzed by LC-MS/MS on a Q Exactive Plus (Thermo Scientific) as previously described (44) ...
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bioRxiv - Plant Biology 2022Quote: ... The peptides were loaded onto a reversed phase trap column (Acclaim PepMap100, 100 μm × 2 cm, nanoViper C18; Thermo Scientific) connected to the C18-reversed phase analytical column (EASY-Column ...
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bioRxiv - Genomics 2022Quote: ... Extractions were centrifuged for 10 min at 17,900 x g and the upper aqueous phase was transferred to new microfuge tubes and the DNA precipitated by adding 0.5 μl GlycoBlue Coprecipitant (Invitrogen AM9515), 0.1 volumes of 3M sodium acetate ...
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bioRxiv - Genomics 2022Quote: ... Extractions were centrifuged for 10 min at 17,900 x g and the upper aqueous phase was transferred to new microfuge tubes and the DNA precipitated by adding 0.5 μl GlycoBlue Coprecipitant (Invitrogen AM9515), 0.1 volumes of 3M sodium acetate ...
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bioRxiv - Immunology 2022Quote: ... Peptides were separated by reverse phase chromatography using a C18 pre-column (Acclaim PepMap 100, 75μm×2cm, Thermo Fisher Scientific) followed by C18 reversed-phase LC column (PepMap RSLC C18 ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was extracted by phase separation and poly(A) selection was performed using Dynabeads mRNA DIRECT Purification Kit (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... The cultures were then grown for ∼3 hours and total RNA was isolated from mid-log phase cultures using TRIzol Max Bacterial Isolation kit (ThermoFisher). RNA was quantified using a Qubit 4.0 fluorometer ...
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bioRxiv - Microbiology 2022Quote: ... Labelled NulOs were analyzed by reverse phase chromatography Orbitrap mass spectrometry (QE plus quadrupole Orbitrap, Thermo Fisher Scientific, Waltham, MA). NulOs were identified according to their mass ...
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bioRxiv - Neuroscience 2023Quote: ... peptides were separated on a 250 mm x 75 μm Acclaim PepMap® C18 reverse phase analytical column (Thermo Scientific) over a 150 minutes organic gradient ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Images of U87vIII and mtDsRed-expressing U87 spheroids were acquired by phase contrast and RFP fluorescence channel using a 4x/0.13 objective with an EVOS FL® Cell Imaging System (Invitrogen) and images of mtDsRed-expressing U87 and GL261 spheroids were acquired by RFP fluorescence channel using a 4x/0.1 objective with a Leica DM IL LED® (Leica ...
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bioRxiv - Microbiology 2023Quote: Peptides for proteome analyses were separated by reverse phase liquid chromatography (LC) on an RSLCnano system (Ultimate 3000, Thermo Scientific) coupled online to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... pH 10) prior to fractionation by high pH reversed-phase chromatography using an Ultimate 3000 liquid chromatography system (Thermo Scientific). In brief ...
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bioRxiv - Neuroscience 2022Quote: ... Peptides (2 μg/ sample) were injected onto a nanoscale C18 reverse-phase chromatography system (UltiMate 3000 RSLC nano, Thermo Scientific) and electrosprayed into an Orbitrap Exploris Mass Spectrometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... 140 μL of the aqueous phase was extracted and transferred to a new tube containing 100 μL isopropanol and 1 μL glycogen coprecipitant (Invitrogen). RNA was pelleted by spinning at 4° C for 45 min ...
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bioRxiv - Microbiology 2022Quote: ... Chromatography employed a reverse phase EASY-Spray™ column (25cm x 75μm ID, PepMap™ RSLC C18, 2μm, ThermoFisher Scientific) connected to an UltiMate™ 3000 RSLCnano HPLC (ThermoFisher Scientific) ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: BM-MSCs in logarithmic growth phase and in good condition were washed twice with PBS (Gibco, Grand Island, NY, USA). Subsequently ...
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bioRxiv - Plant Biology 2024Quote: ... 1.7uM C18 stationary phase (IonOpticks) with either a Vanquish Neo HPLC system or an EASY-nLC 1200 HPLC (Thermo Scientific) coupled via a captive-spray source to a timsTOF pro2 (Bruker Daltonics ...
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bioRxiv - Microbiology 2024Quote: LC-MS/MS analysis was performed using an UltiMate® 3000 nano splitless reversed-phase nanoHPLC (Thermo Fisher Scientific, Dreieich) coupled online to a timsTOF™ pro mass spectrometer (Bruker Daltonik GmbH ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides was dissolved in buffer A (0.1% Formic acid) loading into a a reverse phase trap column (Thermo Scientific Acclaim), the column was connected to a C18-reversed phase analytical column (Thermo Scientific Easy Column) ...
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bioRxiv - Microbiology 2024Quote: Digested peptides were analyzed with 90-minute gradients in C18 nanoflow reversed-phase HPLC (Dionex Ultimate 3000,3500 RSLC nano, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive Orbitrap HF ...
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bioRxiv - Neuroscience 2023Quote: ... 0.1% FA and analyzed by Reversed Phase (RP) LC-MS/MS using an EASY-nLCTM 1200 system (Thermo Fisher Scientific) coupled to an Orbitrap LumosTribridTM mass spectrometer equipped with FAIMS ProTM Interface (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mobile phase B consisted of 20% LC/MS grade and H2O and 80% LC/MS grade acetonitrile (LS122500, Fisher Scientific), and both mobile phases contained 0.1% FA ...
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bioRxiv - Biochemistry 2024Quote: ... deglycosylated GCase and the GCase N370S mutant were solid-phase coated on the bottom of a 96-well ELISA plates (Maxisorp Nunc-immuno plate ...
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bioRxiv - Synthetic Biology 2023Quote: ... The high cell density flask was inoculated using 1% volume from a saturated overnight culture and induced at mid-exponential phase (OD 0.5-0.8) with 1 mM Isopropyl ß-D-1-thiogalactopyranoside (IPTG, Fisher Scientific). The cultures were grown at 37 °C until induction followed by a 5 h growth at 30 °C and left at 18 °C overnight (P1 expression) ...
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bioRxiv - Cell Biology 2023Quote: ... The adipogenesis quantification at the macro-scale X40 for mapping the level of adipogenesis (LOA) follow up using phase-contrast EVOS FL Auto-2 Microscope (Invitrogen) (60) ...
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bioRxiv - Molecular Biology 2023Quote: ... The two pooled mixtures were fractionated into four fractions using PierceTM High pH reverse-phase fractionation kit (Life Technologies, 84868), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Peptides for the qualitative analysis were separated by reversed phase LC on an RSLCnano system (Ultimate 3000, Thermo Fisher Scientific) coupled online to an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) ...