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Citations for Thermo Fisher :
2551 - 2600 of 2674 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 3) and four tyr (clutch no. 7) mutants using the Invitrogen TOPO TA kit according to the manufactures protocol (Invitrogen catalogue no. K4575J10), and Sanger sequenced the extracted plasmids from 6-10 colonies per embryo (Fig ...
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bioRxiv - Developmental Biology 2021Quote: ... qRT PCR reaction was run on a QuantStudio(TM) 7 Flex System in a 96 well format with associated software performing analysis (Applied Biosystems Cat. 4485701).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were washed then incubated in 8 μM of the ROS sensitive probe 5-(and-6)-chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate acetyl ester (CM-H2DCFDA) (Molecular Probes, Eugene Oregon), for 45 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Biophysics 2022Quote: African green monkey kidney cells (COS-7) were cultured at 37°C and 5 % CO2 in DMEM medium containing glutamax (Gibco No. 31966-047), 10 % fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... and using the Applied Biosystems ViiA 7 Real-Time PCR System and the appropriate primers for Taqman assays (Life Technologies; Supplementary Table 1). The relative gene expression was calculated using the 2-ΔΔCt quantification method after normalization to GAPDH values and expressed as fold of levels found in undifferentiated hPSCs cells for cultured cell or in WT mice group for liver analysis.
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bioRxiv - Microbiology 2022Quote: ... The eukaryotic cells were mechanically lysed by vortexing the 15 mL conical tube on setting 7 using a Pulsing Vortex Mixer (Fisher Scientific Cat # 02215375), with two 30 second pulses and 30 second ice incubations after each pulse ...
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bioRxiv - Plant Biology 2022Quote: ... Afterwards the gels were incubated in SyproRuby fixation solution (50% MeOH, 7% acetic acid) for 30 min and incubated overnight in SyproRuby staining solution (Thermo Fisher Scientific - S12000). The following day the gels were washed applying SyproRuby washing solution (10% MeOH ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The grid was blotted for 7 sec at 4°C with 100% humidity and vitrified using a Vitrobot Mark IV (Thermo Fisher Scientific, USA) by plunging into the liquid ethane cooled by liquid nitrogen.
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bioRxiv - Molecular Biology 2022Quote: ... 7 mM (ATP) and 100 mM (Na+) before vitrification by plunge freezing using an Vitrobot Mark IV (Thermo Fisher Scientific, Hillsboro, Oregon, USA) onto Quantifoil R1.2/1.3 copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Genetics 2024Quote: ... and 3.8 μL of water) to determine the additional number of PCR cycles needed for optimal cDNA amplification (Applied Biosystems QuantStudio 7 Flex). We then prepared PCR reactions per total number of barcoded beads collected for each sNucDrop-seq run ...
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bioRxiv - Synthetic Biology 2023Quote: ... we added all four nucleotides at a final concentration of 1 µM (Biotin-7-dATP, Jena Biosciences, and dC-Atto647N or dU-Atto488, Jena Biosciences with respective pair of unmodified nucleotides, Thermo Scientific): [Biotin-7-dATP ...
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bioRxiv - Immunology 2024Quote: ... then collected and plated 10:1 with allogeneic DC-10s in 24-well plates at a concentration of 7 × 105 to 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco, Thermo Fisher Scientific). For CRISPRa experiments ...
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bioRxiv - Cell Biology 2024Quote: ... 500 nM recombinant MEC-12/MEC-7 tubulins and a kinase candidate [500 nM recombinant MBK-2m or 475 nM CDK1/Cyclin B (Thermo Fisher; Cat# PV3292)] were incubated in MEM buffer [100 mM MES ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Cancer Biology 2022Quote: ... HepG2 or MCF-7 cultures were grown for imaging on poly-D-Lysine coated 8-well LabTek II chambered coverglasses (Nunc, Rochester, NY, USA) for 1-2 days ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Cancer Biology 2023Quote: ... fresh sections of each FFPE block were cut on a microtome at 7 μm thickness and mounted on glass slides with polyethylene naphthalate membranes (Thermo Fisher Scientific, LCM0522) and stored in a nitrogen chamber ...
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bioRxiv - Molecular Biology 2023Quote: The mRNA content of AMPKγ3 and TATA-box binding protein (TBP) was determined by real-time PCR using the QuantStudio 7 Flex detection system (Applied Biosystems, CA, USA) as previously described (33) ...
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bioRxiv - Biochemistry 2023Quote: ... at ~10 mg ml-1 concentration and blotted for 5-7 s followed by plunge-freezing into liquid ethane using a Vitrobot MarkIV (Thermo Fisher Scientific, USA) operating at 100 % humidity ...
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bioRxiv - Bioengineering 2023Quote: ... For each sample 2 μg of solubilized silk protein was loaded into a NuPAGE™ 7% Tris-Acetate gel (EA03585BOX, Invitrogen, Waltham, MA) under reducing conditions ...
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bioRxiv - Genetics 2023Quote: ... Blocks were later cut on a cryostat into 7-10 µm sections and placed on Fisherbrand Tissue Path Superfrost Plus Gold slides (Fisher Scientific; Cat# FT4981gplus). H&E staining was performed as previously described(Beedle 2016) ...
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bioRxiv - Biochemistry 2023Quote: ... and transferred onto a 0.45 μm nitrocellulose membrane for 7 min at 20 V with the i-Blot 2 (Thermo Fisher, Waltham, MA, USA). Blocking ...
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bioRxiv - Immunology 2023Quote: ... except for Fig 6 and Fig 7 which the recovered cells were counted by inclusion of counting beads (Accu-Check, Molecular Probes, Thermo Fisher Scientific) in the FACS sample according to manufacturer’s instructions and calculated using the equation ...
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bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA) and Direct-Zol RNA Mini Prep (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... qPCR was performed using a QuantStudio™ 7 Flex Real-Time PCR System in conjunction with a SybrGreen System (Thermo Scientific; Oakville, Canada). All cycle threshold (CT ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time reverse transcription polymerase chain reactions (RT-PCRs) were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Life Technologies). Data were normalized to housekeeping genes (Tubulin ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of RNA were reverse transcribed and amplified using TaqMan™ RNA-to-C T ™ 1-Step Kit kit on the ViiA 7 Real-Time PCR System (Applied Biosystems). TaqMan gene expression assay probes (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: The number of epithelial cells in the Cervix Chip was obtained by digesting the Cervix Chip apical channel at day 7 of differentiation or day 3 on co-culture with bacteria with Collagenase IV (Gibco, Cat. No. 17104019) in TrypLE (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... referred to as ENAS medium (supplementary table 1) PDTs were routinely passaged at intervals of 7 days using TrypLE (Gibco, Cat. No. 12605010) and pipetting to dissociate the cells.
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged for 3 min at 500 g and Accutase was replaced with 500ul assay medium containing 2μM 2’,7’ –dichlorofluorescein diacetate (DCFDA) (Thermo Fisher Scientific, cat.no. D399) and 1:1000 live-dead stain Zombie NIR (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... non-metabolizable glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Thermo Fisher Scientific, cat.no. 11569116). Samples were prepared as described in the section ...
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bioRxiv - Microbiology 2024Quote: ... Transfection of the plasmids listed in Supplementary Table 7 into Expi293F cells was performed according to the manufacturer’s instructions using the Gibco™ ExpiFectamine™ 293 Transfection Kit (Gibco™ A14524). Following transfection ...
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bioRxiv - Neuroscience 2024Quote: Real-Time RT-PCR reactions were performed in a QuantStudio 7 Flex Real-Time PCR System using SYBR green technology (PowerTrack SYBR Green Master Mix, Thermo Fisher Scientific, A46109), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Human embryonic kidney cell line HEK293FT and breast cancer cell line MCF-7 were both cultivated in Roswell Park Memorial Institute medium (RPMI, Thermo Fisher Scientific, Gibco) supplemented with 10% FCS (fetal calf serum ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were maintained in DMEM medium (for both H-4-II-E and HuH-7 cells) supplemented with 10% (v/v) fetal bovine serum (FBS; ThermoFisher, Waltham, MA, USA) and antibiotics (containing 10011U/mL penicillin and 10011μg/mL streptomycin ...
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Impaired yolk sac NAD metabolism disrupts murine embryogenesis with relevance to human birth defectsbioRxiv - Developmental Biology 2024Quote: ... Insoluble proteins and other debris were removed by centrifugation at 12000 rpm for 7 minutes in a microcentrifuge and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, USA) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Samples were boiled for 5 min prior to loading 7 µg of samples on to 4-15% SDS-PAGE gels alongside a molecular weight ladder (Thermo Fisher Scientific, cat# LC5602) and electrophoresis at 200V for 40 min ...
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bioRxiv - Cell Biology 2020Quote: ... Harvested equine explants were allowed to recover in 6-well plates for 3-7 days in the following control medium: DMEM/F12 medium (11330, Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (Seradigm 1500-500 ...
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bioRxiv - Immunology 2020Quote: Quantitative PCR (qPCR) was performed using ViiA 7 Real-Time PCR System and Fast SYBR Green Master Mix reagent (Applied Biosystems, Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1).
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bioRxiv - Molecular Biology 2020Quote: ... and 1 µl of 100 µM bottom splint (IDT) diluted in 7 µl nuclease free H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). The reaction was annealed at 95 °C for 1m following 20 s at 95 °C and decreasing temperature by 1 °C every cycle ...
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bioRxiv - Immunology 2021Quote: Human T cells were differentiated for 6–7 days in a humidified 5% CO2 incubator at 37°C with IMDM (Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2019Quote: ... was added to the cells with complete media for 30 min in 37°C and the caspase-3/7 positive cells were measured on an Attune NXT flow cytometer (Life Technologies, Carlsbad, Calif, USA). For TFH cell sorting ...
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bioRxiv - Cell Biology 2019Quote: 1.5 cm-thick intact human brain slice (Number 7, see Fig. S3) was randomly chosen and passively incubated with 400 ml TO-PRO-3 (T3605, Thermo Fisher, 1:2000 dilution) in PBS at room temperature for 1 week ...
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bioRxiv - Bioengineering 2021Quote: ... Cell nuclei were stained with 10 mM Hoechst 33342 for 7 min followed by mounting using ProLong™ Gold Antifade (ThermoFisher, Waltham, MA, USA). Images were taken using an Olympus FV 1000 confocal microscope.
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bioRxiv - Cancer Biology 2020Quote: ... Transduction of MCF-7 cells and selection of stably transfected clones were performed according to the ViraPower™ Lentiviral Expression Systems user manual (Invitrogen, 25-0501 MAN0000273).
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bioRxiv - Microbiology 2020Quote: ... The sporozoite-infected culture was maintained for 3 or 5 or 7 days after which the cells were fixed with 4% paraformaldehyde (ThermoFisher Scientific: catalogue number 28906) for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...