Labshake search
Citations for Thermo Fisher :
2501 - 2550 of 2674 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... Clonal gp130 KO Huh-7 cells were transduced with this mutant vector and selected and subsequently cultured with neomycin (400 µg/ml; Thermo Fisher Scientific). Short hairpin (sh)RNA knockdown constructs were acquired from the Mission TRC shRNA library (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR analysis was performed using Green PCR Kit (TransGen, Cat# 2-21) on a QuantStudio 7 Flex system (Thermo fisher, USA). The gene-specific primers were listed in Supplementary Table 2 ...
-
bioRxiv - Neuroscience 2024Quote: ... At the end of each qPCR amplifications plots and melt curves (ΔRn vs cycle per well) were automatically calculated by the Quant Studio 7 Pro qPCR instrument (Thermo Fisher Scientific). Relative circRNA levels were quantified directly from the Ct average’s as shown before.17,20
-
bioRxiv - Biochemistry 2024Quote: ... The grids were blotted using filter paper for 7 seconds at 4°C under 100% humidity using Vitro Mark IV (Thermo Fisher Scientific). Subsequently ...
-
bioRxiv - Genetics 2024Quote: ... RNA was extracted from 4 or 5 pools of 10 embryos at 7 dpf and 14 dpf using Trizol (Life Technologies Europe) and purified using the RNeasy Mini Kit (Qiagen ...
-
bioRxiv - Molecular Biology 2024Quote: ... Sections were cut at 7 µm thickness and mounted onto pre-charged slides (#12-550-15 Superfrost Plus slides, Fisher Scientific, USA). For immuno-fluorescence (IF) ...
-
bioRxiv - Neuroscience 2024Quote: ... tissues were minced in 2 mm sections using a razor blade in ice-cold B1 buffer (Hanks Balanced Salt Solution with 10 mM HEPES, pH 7; Thermo Fisher Scientific). Subsequently ...
-
bioRxiv - Cell Biology 2024Quote: ... Intracellular 7-ketocholesterol was measured by fixing cells in 4% paraformaldehyde and staining with 7-ketocholesterol monoclonal antibody at 1:50 (3F7, Invitrogen, MA5-27561) diluted in 1X permeabilization buffer (Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... The cDNA was further used for Real-time quantitative polymerase chain reaction (qPCR) performed on QuantStudio 7 Flex system (Invitrogen™ Life Technologies) using Fast SYBR Green Master Mix (Life Technologies).
-
bioRxiv - Cell Biology 2020Quote: ... Cells were washed once and resuspended with 200 µg/mL PureLink™ RNase A and eBioscience™ 7-AAD viability staining solution (Thermo Fisher). After 30 min of incubation at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transfected using JetPEI® DNA transfection reagent (CHO dhFr- cells, Polyplus Transfection, Illkirch-Graffenstaden, France) or lipofectamin®2000 reagent (COS-7 and MDCK cells, Invitrogen, Carlsbad, US). Experiments were performed 24h (TIRF imaging ...
-
bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
-
bioRxiv - Immunology 2020Quote: Quantitative PCR (qPCR) was performed using ViiA 7 Real-Time PCR System and Fast SYBR Green Master Mix reagent (Applied Biosystems, Thermo Fisher Scientific). Primer sequences are listed in Supplemental Table 1).
-
bioRxiv - Microbiology 2022Quote: A549 and Huh-7 cells were transfected with CRISPR/Cas9 RNP (IDT, Coralville, Iowa) by nucleoporation (NEON Electroporation System, Thermo Fisher, Massachusetts, USA) using the following parameters ...
-
bioRxiv - Molecular Biology 2020Quote: Total RNAs from 7-d-old seedlings grown on 1/2 MS with or without 1 μM ABA were extracted using TRIzol (Invitrogen, Carlsbad, CA, USA) or Universal Plant Total RNA Extraction Kits (Spin-column)-I (BioTeke ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cover slips are mounted on top of a drop (7-8 uL) of ProLongTM Diamond Antifade Mountant (Thermo Fisher Scientific, Canada. Catalog# P36970).
-
bioRxiv - Immunology 2021Quote: ... Quantitative PCR was performed on a QuantStudio 7 Flex Real-Time PCR System using the QuantStudio Real-Time PCR Software v1.3 (Life Technologies, Carlsbad, CA, USA).
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The activity of the major drug metabolizing enzyme CYP3A4 supported by WT or POR-284T was tested using the fluorogenic substrates [BOMCC (7-Benzyloxy-4-trifluoromethylcoumarin) (Invitrogen Corp, Carlsbad, CA) as described earlier (Flück et al. ...
-
bioRxiv - Microbiology 2019Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 20 s and was run on QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems™). Provided that the efficiencies of the reactions were between 90% and 110% ...
-
bioRxiv - Neuroscience 2019Quote: ... Total protein was transferred to a 0.45 μm pore nitrocellulose membrane at 20 V for 7 minutes using iBlot Gel Transfer Nitrocellulose Stacks (NR13046-01, Invitrogen, Waltham, MA, USA) and an iBlot machine (Invitrogen ...
-
bioRxiv - Bioengineering 2021Quote: ... Real-time polymerase chain reaction (real-time PCR) was performed on duplicates using QuantStudio 7 Flex (Applied Biosystems, Thermo Fisher Scientific, Waltham, USA). The relative gene expression was identified using 2-ΔΔCt value (72 ...
-
bioRxiv - Biochemistry 2021Quote: ... 5 µL of each SF was diluted 7-fold in water and used for a Bradford protein assay (Thermo Fisher Scientific, Rockford, IL).
-
bioRxiv - Cell Biology 2021Quote: ... The extent of cofilin1 transcript knockdown was assessed by quantitative real-time PCR performed on a QuantStudio 7 Flex system (Thermo Fisher Scientific #4485701), using the Power SYBR Green PCR master mix (Thermo Fisher Scientific #4367659) ...
-
bioRxiv - Bioengineering 2021Quote: ... and the solution was added to a Custom RT2 PCR Array 384-well plate and qPCR program was run using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems 4485701). Data analysis was performed on https://geneglobe.qiagen.com/us/analyze ...
-
bioRxiv - Cancer Biology 2020Quote: ... 500 µL of Herpes solution and 2.5 mL of 100 mM CaCl2), staining with Annexin V PE (BD Pharmagen™, BD Biosciences, CA, US and 1 mM 7-Aminoactiomycin D (Thermo Fisher Scientific) and analysis by Flow Cytometry using a BD LSRFortessa X20.
-
bioRxiv - Neuroscience 2020Quote: Levels of gene expression in the tissue was quantified using qPCR using either the Viia 7 Real-Time PCR system (Applied Biosystems, CA, USA). Reactions were made using ...
-
bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
-
bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific, Thermo Fisher Scientific) and dried ...
-
bioRxiv - Biophysics 2022Quote: ... A 16-hour dialysis was set-up by placing the reconstitution mixture in a 7 kDa MWCO Slide-A-Lyzer MINI Dialysis Device (Thermo Scientific cat # 69560) and placed in a flotation device in high-salt buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4ºC) and incubated with 50 μL of 5 μM DAF-FM (4-amino-5methylamino-2’,7’-dichlorofluorescein diacetate, Life Technologies, Eugene, OR, USA) diluted in 1X PBS for 30 min at 34 °C ...
-
bioRxiv - Bioengineering 2022Quote: ... MCF-7 breast cancer cells were cultured and stained with a live fluorescent dye (CellTracker™ Red CMTPX dye, Invitrogen™, Carlsbad, USA). WBCs were isolated from human whole blood and also stained with a live cell marker (10 μM CMTPX ...
-
bioRxiv - Cell Biology 2022Quote: ... Samples were run in triplicates and analyzed on the QuantStudio™ 7 Flex Real-Time PCR 384-well System (ThermoFisher Waltham, MA, US). Data was normalized to the housekeeping genes GAPDH (Hs99999905_m1) ...
-
bioRxiv - Genetics 2022Quote: ... Sperm spreads were obtained by dissecting 10-12 male worms in 7 microliters of egg buffer (Edgar 1995) on ColorFrost Plus slides (Fisher Scientific, 12-550) that were additionally coated with poly-L-lysine (Sigma Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... 2.7 μg envelope plasmid pMD2G and 8 μg target plasmid encoding shRNA or lentiCRISPRv2 encoding sgRNA using Lipofectamine® 2000 (Thermo Fisher Scientific). Lentivirus supernatants were collected at 36 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... qPCR was carried out using PerfeCTa® SYBR® Green SuperMix Reaction Mix (Cat. no. 95055-500) in real time PCR system Quant Studio 7 PRO (Applied Biosystems). For RT-PCR analysis of splicing ...
-
bioRxiv - Developmental Biology 2022Quote: ... we found that poly-lysine treating the coverslips was not necessary for immobilization of the worms in most cases as long as the liquid under the agarose pad is minimal.) 7-9% agarose pads (Invitrogen, cat no. 16500500) were gently placed over the top of the worms and excess liquid was wicked away using Whatman paper ...
-
bioRxiv - Developmental Biology 2019Quote: ... one midgut and 7 µL of Schneider’s medium were placed in each well and topped with a coverslip (Fisher Scientific, #12-545-81). Midguts were imaged using an inverted Zeiss LSM 880 immediately after mounting.
-
bioRxiv - Neuroscience 2019Quote: ... Supernatant was then removed and the pellet was gently resuspended in a total volume of 800µl prior to 7-AAD (Thermo Fisher, 00-6993-50) staining and FACS to further purify the nuclei for sequencing ...
-
bioRxiv - Immunology 2019Quote: ... incorporating a T7 promoter sequence that was used to generate synthetic segment 7 vRNA using the TranscriptAID T7 high yield transcription kit (Thermo Fisher Scientific; K0441). Synthetic vRNA was purified using an RNAeasy column and used as a reference standard for reverse transcription and qRT-PCR analysis.
-
bioRxiv - Immunology 2021Quote: Total RNA of THP-1 monocytes derived macrophages cultured in different circumstance for 7 days was digested from 6-well plates using trypsin and then TRIzol™ Reagent (Cat. #15596026, ThermoFisher Scientific, USA) was added ...
-
bioRxiv - Bioengineering 2020Quote: ... Scaffolds were washed in PBS at every timepoint (day 1, 4, 7, 14, 28) and placed in a solution of alamarBlue® (Invitrogen, California, USA) in an incubator at 37°C on a shaker ...
-
bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
-
bioRxiv - Pathology 2021Quote: ... The qRT-PCR was performed with the cDNAs and the expression of genes of interest was evaluated using TaqMan primer/probe sets with QuanStudio 7 Flex (Applied Biosystems, Carlsbad, CA). GAPDH and RPLPO were used as housekeeping genes.
-
bioRxiv - Genomics 2021Quote: ... and 3.8 μL of water) to determine the additional number of PCR cycles needed for optimal cDNA amplification (Applied Biosystems QuantStudio 7 Flex). We then prepared PCR reactions per total number of barcoded beads collected for each sNucDrop-seq run ...
-
bioRxiv - Genomics 2019Quote: Consecutive sections of the FFPE block were taken on a microtome at 7 μm thickness and mounted on glass slides with polyethylene naphthalate membranes (Thermo Fisher Scientific #LCM0522). Slides were stored overnight in a nitrogen chamber ...
-
bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
-
bioRxiv - Cell Biology 2020Quote: ... A total of 10ng cDNA for each sample was used and all RT-qPCR reactions were performed on a ViaA 7 Real-Time PCR System (Life Technologies, Carlsbad, CA) using Brilliant II SYBR Green QPCR Master Mix (Agilent ...
-
bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
-
bioRxiv - Cell Biology 2021Quote: ... and HT-1080 cells were obtained from National Center for Cell Science (NCCS, Pune, India) with MCF-7 cultured in MEM (Gibco, Cat #61100-061), T-47D and ZR-75-1 were cultured in RPMI-1640 (Gibco ...
-
bioRxiv - Biophysics 2020Quote: ... Subsequently, protein samples were washed once using Zeba™ Spin desalting columns (7 kDa MWCO, 2 mL) (Thermo Fisher Scientific, Waltham, MA, USA) equilibrated with degassed buffer A ...