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Citations for Thermo Fisher :
2201 - 2250 of 2521 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with solution containing 468 μl of 50 mM ammonium bicarbonate and 7 μg of Trypsin (Thermo Fisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
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bioRxiv - Microbiology 2024Quote: ... 7 μl per injection was loaded onto a 2 mm by 0.3 mm Acclaim PepMap C18 trap column (Thermo Fisher Scientific) in 0.1% TFA at 15 μl/min before the trap being switched to elute at 0.25 μl/min through a 50 cm by 75 μm EASY-Spray C18 column ...
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bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (qPCR) was performed using standard SYBR green reagents and protocols on a QuantStudio 7 Real-Time PCR system (Applied Biosystems). All reactions were performed in technical triplicates ...
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bioRxiv - Cell Biology 2024Quote: COS-7 cells were grown at 37°C with 5% CO2 in complete Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific, USA) containing 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of a 1:10 dilution of 1st strand cDNA was assayed by qPCR on Applied Biosystems Viia-7 instruments in 10 µL reactions with PowerUp SYBR Green master mix (Applied Biosystems) according to manufacturer recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Developmental Biology 2024Quote: ... Real-time PCR amplification and fluorescence detection were carried out using a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher). An initial step (95 °C ...
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bioRxiv - Genomics 2024Quote: ... was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system using standard settings (Applied Biosystems). Expression of genes was normalised to two housekeeping genes (Bactin ...
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bioRxiv - Cancer Biology 2024Quote: ... Biozym Scientific GmbH) was used and analysis was done on a ViiA 7 Real-Time PCR System (4453536, Thermo Fisher Scientific). mRNA levels were normalized to the geometric mean of Cyclophilin A and hypoxanthine guanine phosphoribosyl transferase and/or 18S ...
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Plant Biology 2020Quote: The procedure for cloning KDR CDS was as described before but with the use of the primers listed in Supplemental Table 7 and cloned into the Gateway vector pDONR221 (Thermo Fisher Scientific). The competent cells transformed with the entry vectors were selected for growth on the antibiotic kanamycin (50 µg/ml) ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was further used for Real-time quantitative polymerase chain reaction (qPCR) performed on QuantStudio 7 Flex system (Invitrogen™ Life Technologies) using Fast SYBR Green Master Mix (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis was performed by quantitative real-time RT-PCR (qRT-PCR) using SYBR Green PCR Master Mix and the ViiA™ 7 Real-Time PCR System (Applied Biosystems). For each target gene ...
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).
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bioRxiv - Genetics 2021Quote: ... Blocks were later cut on a cryostat and 7-10 μm thick sections were placed on SuperFrost Plus slides (Cat#FT4981GLPLUS; ThermoFisher; Waltham, MA). Hematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Genetics 2021Quote: ... The purified products were separated and detected by capillary electrophoresis (CE) using the ABI 3130 Genetic Analyzer with POP-7 polymer and GeneScan-120 Liz (Thermo Fisher Scientific). The raw data were analyzed using the GeneMapper ID V3.2 software (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... cyli kidneys was determined by quantitative real-time PCR performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific) using the default program ...
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bioRxiv - Molecular Biology 2020Quote: ... was added to bind excess dye and the resultant solution was passed through 7 K MWCO Zeba Spin Desalting Columns (Thermo Fisher Scientific) to remove excess dye ...
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Microbiology 2020Quote: ... The cells were homogenized using an Avestin EmulsiFlex homogenizer and lysed using sonication (programme 7, Fisher Scientific™ Model 705 Sonic Dismembrator) and the resultant lysate was transferred into SS34 tubes for further centrifugation at 18,000 rpm (JA-25.50 rotor ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems QuantStudio Flex 7 Real Time PCR System (Thermo Fisher Scientific). The ΔΔCT method was used for relative quantification of target mRNA expression and normalized to Rn18s (18S rRNA ...
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bioRxiv - Developmental Biology 2020Quote: ... samples from pools of 20 medaka hatchlings (stage 40) and human fibroblasts (7×105 cells) were lysed with RIPA Lysis and Extraction Buffer (Thermo Scientific, #89900). Samples were purified with methanol/chloroform protein extraction protocol according to (Wessel & Flügge ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems Quant Studio Flex 7 Real Time PCR System (Thermo Fisher Scientific). The ΔΔCT method was used for relative quantification of target mRNA expression level normalized to Rn18s (18S rRNA) ...
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bioRxiv - Developmental Biology 2020Quote: ... QPCR on technical triplicates for each sample was performed on 5 ng of relative RNA using Takyon Low ROX SYBR 2X MasterMix blue dTTP (Takyon) on a ViiA™7 thermal cycler (Applied Biosystems). Ct values of all genes analysed were normalized to the geometrical mean of Ct values of three housekeeping genes (actinb2 ...
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bioRxiv - Neuroscience 2022Quote: Bacterial load was determined as described previously by Nadkarni et al.(Nadkarni et al., 2002) using Taqman and ViiA™7 Real-Time PCR System (Life Technologies). PCR consisted of 40 cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... PR2 or GFP PCR amplicons of about 500 pb (Supplementary Table 7) were used as templates to produce random labelled probes using the Decalabel DNA Labelling kit (Thermo Fisher Scientific) and [α32P]-dCTP according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... we performed a Gibson Assembly to insert the Kate2 expressing oligo ordered as a gene string from Invitrogen (Supplementary Data Table 7).
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bioRxiv - Developmental Biology 2019Quote: ... Gene expression analysis was performed using PerfeCTa SYBR Green FastMix (Quanta, Cat No. 95073) on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific). The data presented correspond to the mean 2-ΔΔCt after being normalized to the geometric mean of β-actin ...
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bioRxiv - Bioengineering 2019Quote: ... Culture medium for MDA-MB-231 (HTB-26™, ATCC, US), and MCF-7 (HTB-22D™, ATCC, US) culturing in DMEM medium (Gibco−) supplemented with 10% PBS and 1% 100x antibiotic-antimycotic (Anti-Anti ...
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bioRxiv - Neuroscience 2019Quote: ... while nine tenth of the samples were analyzed via Coomassie staining after separation on 7% NuPAGE™ Tris-Acetate protein gels (Thermo Fisher) in NuPAGE™ Tris-Acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2019Quote: ... Each sample was quantified by targeting tp0574 through qPCR using a 96-well reaction plate with a ViiA 7 Real-Time PCR System (Applied Biosystems, USA) according to the protocals already established in the laboratory [31] ...
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bioRxiv - Biochemistry 2019Quote: The N30 Random DNA pool and cDNA from rounds 7-to-9 were cloned into TOPO™ TA Cloning™ Kit (Invitrogen) following manufacturer’s specifications ...
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bioRxiv - Immunology 2019Quote: ... MDSCs and monocytes were incubated at a concentration of 1-7×105 cells/well in FluoroBrite Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Waltham, MA) supplemented with 10% human serum ...
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bioRxiv - Neuroscience 2019Quote: Culture medium from 7 div OBSCs was assayed for human Aβ1-40 and Aβ1-42 using commercially available ELISA kits (Life Technologies: KHB3441 and KHB3481). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... The retina was then transferred to a microcentrifuge tube and incubated for 7 minutes at 37°C with an activated papain dissociation solution (87.5 mM HEPES pH 7.0 (Thermo Fisher Scientific, cat. #15630080), 2.5 mM L-Cysteine (MilliporeSigma ...
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bioRxiv - Genetics 2020Quote: ... Blocks were later cut on a cryostat and 7-10 µm thick sections were placed on SuperFrost Plus Gold slides (ThermoFisher; Cat# FT4981GLPLUS). Hematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded onto 384-well plates in triplicate and run on a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific) where gene expression was normalized to β-actin and determined using the ΔΔCt formula.
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bioRxiv - Cancer Biology 2019Quote: Ccr2+/+ or Ccr2−/− cancer cells were isolated from tumors and sorted as EpCAM+CD45-CD31- live cells (using 7-AAD Viability Staining Solution, Thermo Fisher Scientific) four weeks after orthotopic transplantation into the mammary glands of wild-type C57BL/6 mice ...
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bioRxiv - Cell Biology 2020Quote: ... The mEGFP-tagged Tom20 line was transfected with mCherry-Mito-7 construct (Michael Davidson, addgene #55102) using 6 μl per well of transfection mixture containing 25 μl Opti-MEM (ThermoFisher #31985-070), 1.5 μl GeneJuice (Millipore #70967 ...
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bioRxiv - Genomics 2020Quote: ... PMPs were harvested from suspension during medium exchange and plated in microglia differentiation media over 7-14 days to produce microglia like cell monocultures (Neurobasal (Life Technologies 21103049) supplemented with Gem21 NeuroPlex without Vitamin A (GeminiBio ...
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... and the pellet was gently resuspended in 800 μl of wash buffer before 7-aminoactinomycin D (Thermo Fisher Scientific, 00-6993-50) staining and fluorescence-activated cell sorting (FACS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells (1 × 107) from passage 5-7 were used for transplantation after staining with carboxyfluorescein succinimidyl ester (CSFE) (Invitrogen, Carlsbad, California, USA) on the day of the transplantation ...
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bioRxiv - Cell Biology 2021Quote: ... we performed a TaqMan quantitative real-time PCR approach to quantify sjTREC expression (signal joint excision circles produced during T-cell development) using ViiA™ 7 Real-Time PCR System (Life technologies). TaqMan qPCR was performed on 75-100 ng DNA in a 12 μl reaction mixture containing 18 µM of each primer for sjTREC (5’-TCGTGAGAACGGTGAATGAAG-3’ and 5’-CCATGCTGACACCTCTGGTT-3’ ...