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Citations for Thermo Fisher :
2401 - 2450 of 2674 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... Cells were passaged on day 3 to maintain sub-confluent culture conditions and analyzed by flow cytometry on day 7 using an Attune NxT (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: PBMCs were plated into a 10 cm dish at 7×107 cells/dish and adhered for 2 hours in AIM-V (Gibco-BRL, CA). The nonadherent cells were removed gently and frozen as the source of lymphocytes for co-culture use ...
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bioRxiv - Developmental Biology 2022Quote: ... but the blastocysts were incubated in 10 μM 4-chloromethyl-6,8-difluoro-7-hydroxycoumarin (CMF2HC; Cell Tracker Blue, Life Technologies, Carlsbad, USA). Fluorescence intensity for GSH was measured under a Nikon scanning confocal microscope with a filter at 371 nm excitation and 464 nm emission ...
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bioRxiv - Pathology 2022Quote: ... All RNA extracts were eluted in 60 μL of diethyl pyrocarbonate (DEPC)-treated water for storage at −70°C prior to quantification and were tested in triplicate using an Applied Biosystems ViiA 7 RT-qPCR machine (Thermo Fisher Scientific). Viral RNA levels were calculated in RNA copies by comparing the average of each triplicate from a sample to the standard curve generated with each PCR plate ...
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bioRxiv - Immunology 2022Quote: The production of NO was evaluated using 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA)(D23844; Invitrogen, Waltham, MA). Following leukocyte isolation ...
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bioRxiv - Neuroscience 2019Quote: ... The washed pellet was either stored at −20 °C overnight or resuspended in 7-12 ml of inclusion body solubilization reagent (Thermo Scientific #78115). The protein suspension was shaken for 30-40 min at 20 °C and then ultracentrifuged at 35,000 x g for 20-30 min at 4 °C.
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bioRxiv - Cell Biology 2019Quote: MCH2-10 WT and PWS1-7 neural progenitor cells were generated with dual SMAD inhibition approach using PSC Neural Induction medium (ThermoFisher Scientific, A1647801) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCRs were performed on a QuantStudio 7 Flex Real-Time PCR System and threshold cycle (CT) values were determined from duplicate reactions using QuantStudio software (Thermo Fisher Scientific).
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bioRxiv - Systems Biology 2019Quote: Determination of intracellular levels of total ROS was carried out by flow cytometry using 2’,7’-dichlorodihydrofluorescein diacetate (DCH2FDA, Thermo Fisher Scientific), as previously described (Amara et al ...
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bioRxiv - Synthetic Biology 2020Quote: ... The aqueous layer was removed and subjected to another round of phenol chloroform extraction with 7 mL phenol chloroform (Fisher Scientific #BP1752I). The aqueous layer was then decanted and subjected to an isopropanol precipitation (41% final volume isopropanol ...
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bioRxiv - Immunology 2020Quote: ... or aged (26-28 mo) female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco, 11995-073) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... with the substrate tertiary-butyloxycarbonyl-leucine-methionine-7-amino-4-methylcoumarin (abbreviated to t-BOC-Leu-Met-CMAC, or in short: CMAC) (Invitrogen, Carlsbad, CA), at a concentration of 50 μM in the culture medium ...
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bioRxiv - Microbiology 2020Quote: Amount of ROS in dissected guts (from 10 animals) was estimated using 2’,7’-Dichlorodihydrofluorescein diacetate (H2DCFDA, C6827, ThermoFisher Scientific, MA, USA), following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Developmental Biology 2020Quote: ... Genital ridges from 1-2 litters (7-8 embryos per litter) were dissected out and digested at 37°C for 3 min using TrypLE Express (Thermo Fisher Scientific). For E9.5 and E10.5 stages ...
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bioRxiv - Genomics 2021Quote: ... with random hexamer primers and expression levels were quantified in the QuantStudio™ 7 Flex Real-Time PCR machine (Thermo Fisher Scientific) using Power SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Cells were seeded at a density of 2.7×104 cells/cm2 and cultured in William’s Medium E (Biochrom, Berlin, Germany) containing 10% (v/v) FCS (Life Technologies, Darmstadt, Germany), 5 mg/ml insulin (Sigma Aldrich ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was extracted from Arabidopsis leaf tissues grown under an 8-h photoperiod or cotyledons from etiolated seedlings grown in dark for 7 d by TRIzol (Thermo Fisher Scientific) followed by DNase treatment at 37 °C for 30 min ...
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bioRxiv - Immunology 2019Quote: ... Briefly, COS-7 cells (ATCC, #CRL-1651) or B16F1 cells (ATCC, #CRL-6323) were transiently transfected using Lipofectamine 3000 (Thermo Fisher, #L3000008) with plasmid DNA encoding either mHEL-GFP ...
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bioRxiv - Molecular Biology 2019Quote: ... The retina was then transferred to a microcentrifuge tube and incubated for 7 minutes at 37°C with an activated papain dissociation solution (87.5 mM HEPES pH 7.0 (Thermo Fisher Scientific, cat. #15630080), 2.5 mM L-Cysteine (MilliporeSigma ...
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bioRxiv - Biochemistry 2020Quote: ... QPCR was performed on standards of diluted genomic DNA and cDNA products using specific primers (Supplemental table 5) on a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Galnts gene copy numbers were calculated using the genomic DNA as a standard curve and variation between biological replicate was normalized using Actb expression level.
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bioRxiv - Physiology 2021Quote: Total RNA from around 20 whole flies of both sexes at 4-7 days old was extracted using a PureLink RNA Mini Kit (Cat# 12183020, ThermoFisher Scientific, USA). Reverse transcription (RT ...
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bioRxiv - Biochemistry 2021Quote: ... The qPCR reactions were performed in 96-well plates using a ViiA™ 7 Real-Time PCR System (Applied Biosystems, ThermoFisher Scientific) in 20 μL reaction volumes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and ibuprofen (CAS 15687-27-1, purity ≥ 99%), and potassium chloride (KCl, CAS 7447-40-7, purity ≥ 99.7%) were purchased from Thermo Fisher (Waltham, MA).
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bioRxiv - Molecular Biology 2021Quote: ... followed by separation on 20% 7 M urea SDS-PAGE gel and immunoblot assay with anti-Biotin antibody (Thermo Fisher Scientific, 692033).
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cell Biology 2020Quote: ... which was conjugated at a 7:1 molar ratio with the cleavable EZ-Link Sulfo-NHS-SS-Biotin (#A39258, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxy-d-glucose (2-NBDG) was purchased from Invitrogen (Carlsbad, CA, USA). Trypsin-EDTA solution was purchased from GIBCO BRL (Grand Island ...
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bioRxiv - Cell Biology 2021Quote: Cell cultures for 2D experiments were manually seeded with multichannel pipettes at 3500 (MCF-7 and MDA-MB-231) or 2000 (NHDF) cells per well into tissue culture treated 96 well plates (ThermoFisher Scientific #167008) in 150 μL culture media.
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: Bone marrow was collected from tibias and femurs of 4-6 mo female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco,11995-073) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: Detection of VNNV and TiLV was performed using the Promega GoTaq Probe 1-step RT-qPCR System and both were performed in the QuantStudio 7 (Applied Biosystems, UK) as follows ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Immunology 2020Quote: ... 1 × 105 MCF-7 cells were transfected with appropriate plasmid combinations using Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: The images for neurite outgrowth analysis were taken on day 7 of differentiation using CellInsight High Content Imaging Platform (ThermoFisher, CX7A1110/CX7B1110) and then analysed using Columbus (Perkin Elmer) ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were blocked in 7% milk in 1xTBS and 0.01% Tween-20 and streptavidin-HRP immunostaining (1:5000, Invitrogen cat. #19534-050) was performed at room temperature for 1h in blocking solution ...
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bioRxiv - Molecular Biology 2022Quote: ... 95°C for 20sec then 40 cycles of 95°C for 3sec and 58°C for 45sec on the QuantStudio 5 and 7 qRT-PCR systems (Applied Biosystems, CA). A known negative sample and non-template control (NTC ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Biochemistry 2022Quote: ... Free dye was removed from labeled protein by buffer exchanging labeling reactions three times using a ZebaTM Spin Desalting Column (7 kDa MWCO, Thermo Scientific #89890) into 50 mM Tris-HCl (pH 7.9) ...
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bioRxiv - Biochemistry 2022Quote: ... Gen Bank KX197192) was propagated in Aedes albopictus C6/36 cell line for 7 days in Leibovitz-15 medium (Gibco #41300–039) pH 7.4 supplemented with 5% fetal bovine serum ...
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bioRxiv - Bioengineering 2022Quote: ... Cultures were washed three times with washing solution and then incubated for 30 min at room temperature on the OrganoFlow (7°, 2-min intervals) with Alexa Fluor 488 (#a32731; Invitrogen; 1:250) and Alexa Fluor 647 (#a31571 ...
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bioRxiv - Genomics 2022Quote: ... was carried out on a StepOnePlus 96-well or a QuantStudio 7 Flex 384-well Real-Time PCR System (Thermo Fisher Scientific) loading 20-25ng cDNA /well and using TaqMan Fast Advanced Master-Mix (Thermo Fisher Scirentific ...
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bioRxiv - Immunology 2022Quote: Real-time RT-PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (ThermoFisher Scientific #4368708). Primers for HA (forward 5’-AAACTCTTCGCGGTCTTTCCA-3’ ...
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bioRxiv - Microbiology 2022Quote: Sucrose gradient purified fractions of yeast-derived PV-3 SC8 were pooled and buffer exchanged into PBS + 20mM EDTA (pH 7) using Zeba Spin Desalting Columns with a 7K molecular weight cut-off (MWCO) (Thermo Fisher Scientific) and concentrated using Amicon Ultra centrifugal filter devices (100 kDa MWCO ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed three times with unsupplemented neurobasal media and loaded with 200μM 2’,7’-dichlorofluorescein diacetate (DCFH-DA) fluorescent dye (Thermo Fisher Scientific, MA) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Plant Biology 2022Quote: ... Mock-treated and infected roots of WT and HvMPK3 KO plants were incubated in 30 μM 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA; Cat no. C6827, Invitrogen™, USA) and PI (1 mg.ml-1 ...
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bioRxiv - Microbiology 2022Quote: ... at 750 nm of 0.3 and monitored for 7 days by measuring OD at 750 nm every 24 hours using a GENESYS 10S UV-Vis Spectrophotometer (Thermo Scientific, Germany). For PCC 6803 ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR of diluted ChIP DNA and corresponding input DNA was performed on ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Primer sequences used for ChIP are listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...