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Citations for Thermo Fisher :
2151 - 2200 of 2521 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Lysosomal mass was estimated by incubating cells under various genetic perturbations (7 days after sgRNA transduction) with LysoTracker Red DND-99 at 100 nM (Invitrogen; L7528). After incubation at 37ºC for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl was loaded on to NuPAGE 4 to 12% Protein Gels with 7 μl of 4X NuPAGE LDS sample buffer as described by the manufacturer (Invitrogen: NP0322BOX) and run for 2 h at 100 V ...
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bioRxiv - Neuroscience 2023Quote: ... and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713; Integrated DNA Technologies) were run on a ViiA 7 Real-Time PCR system (Applied Biosystems) as described in the instructions for use of the CDC 2019-Novel Coronavirus (2019-nCoV ...
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bioRxiv - Neuroscience 2023Quote: Third instar larval brains were dissected in phosphate buffered saline (PBS) and fixed with 4 % formaldehyde (FA) pH 7 (Thermo Scientific)/PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was performed by standard TaqMan Assay on either the QuantStudio 7 or Quantstudio 5 Real-Time PCR platform (Applied Biosystems). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Following EDTA dissociation crypts were incubated at 37°C for 7 minutes in TrypLE Express (Thermo Fisher Scientific, 12-604-021) and DNase I (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... proteins were dialyzed overnight using a 7 or 10 kDa molecular weight cut-off Slide-A-Lyzer dialysis cassette (Catalog No. 66380, Thermo Scientific) in high salt buffer (500 mM NaCl and 20 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR reactions (all samples are run in triplicate) were carried out on the QuantStudio™ 7 Flex system (Applied Biosystems), and results were calculated by the delta-delta Ct (ΔΔCt ...
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was run in triplicates using 2-5 ng cDNA in a 384 well plate and the qPCR was performed using VIIA 7 real time PCR instrument from Applied Biosystems.
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bioRxiv - Cancer Biology 2023Quote: Cell viability in untreated tumor organoids was analyzed on day 7 using the LIVE/DEADTM Cell Imaging Kit (Invitrogen, Karlsruhe, Germany) according to the manufactureŕs instructions for exemplary cases.
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Plant Biology 2023Quote: Total protein isolated from the light-grown 7-day-old seedlings of each sample using a plant protein extraction kit from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2023Quote: ... beads were resuspended in 20 µl of Elution Buffer mixed with 7 µl of 4X LDS sample buffer (Thermo Fisher, # NP0007) and 3 µl of 10X reducing agent (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... Hippocampi of male and female E17 mouse brains from a single litter (7-9 embryos) were dissected in HBSS (ThermoFisher Scientific) supplemented with 1% L-glutamine ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... The medium was changed every day and cells were passaged at 70%-80% confluency every 5-7 days using PBSCa-/Mg- (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... cells were reverse-transfected into an arrayed 384- well plate library containing small interfering RNA (siRNA) or miRIDIAN microRNA mimic constructs (Horizon Discovery, Supplementary Tables 1-7) using Opti-MEM (Gibco 31985070) and Lipofectamine RNAiMAX transfection reagent (Invitrogen 13778150) ...
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bioRxiv - Plant Biology 2024Quote: ... Ten-μg protein of each sample were separated by electrophoresis in 15% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Roth) for 7 min at 20V using the iBlot 2 Dry Blotting System (ThermoFisher Scientific), before blocking in TBS-T with 5% (w/v ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (4368708, ThermoFisher Scientific). Primers for HA (5’- AAACTCTTCGCGGTCTTTCCA-3’ sense sequence and 5’-GATAAGGTAGCTTGGGCTGC-3’ antisense sequence) ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 99 °C with shaking for 7 min and 25 μg of protein per sample was analysed with 4-12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 mM sodium citrate pH 5.0 buffer containing 7 M urea and 10.5 mM EDTA with 1 U RNase T1 (ThermoFisher Scientific, Waltham, MA) at 55°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was performed with 2.5 ng of DNA in triplicates using PowerSYBR Green PCR master mix at Quant Studio 7 qPCR machine (both Applied Biosystems) according to the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... Movies were collected on a Titan Krios G3i at spot size 7 with a Falcon 4 Direct Detector (Thermo Fisher Scientific) equipped with an energy filter set to 20 eV ...
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bioRxiv - Biophysics 2023Quote: ... The cell-culture medium contained a 1:1 mixture of Eagle’s minimal essential medium (EMEM; for MCF-7) and F12 (Invitrogen, Waltham, MA) with a 2% B-27 supplement (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proliferation was assessed 7 days after treatment with respective drugs using CellTiter-Glo® luminescent Cell Viability Assay (Fisher Scientific, PRG9242). The luminescence signal was measured in a luminometer ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Cell Biology 2023Quote: ... BAL samples were then centrifuged at 3,000 g for 20 min to collect cells and ApoBDs for staining with a combination of SR-DEVD-FMK (caspase 3/7 activity detection dye, Thermo Fisher), annexin A5-V450 and TO-PRO-3 in 1× A5 binding buffer at room temperature for 10 min ...
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bioRxiv - Genomics 2023Quote: The RT-qPCR reactions were carried out on 384-well plates using a QuantStudio 7 Flex Real- Time PCR System (ThermoFisher #4485701) using 10 µl reactions (2 µl of cDNA 3.7 ng/µl ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... subsequently buffer exchanged by using 7 K MWCO Zeba Spin Desalting Columns and the concentration was measured by Nanodrop One (Thermofisher Scientific).
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bioRxiv - Biophysics 2023Quote: 7 μM Aβ40 was incubated with varying stoichiometries of claramine and 5% PEG (50% w/w 10 kDa, Thermo Fisher Scientific) in the presence of 20 mM ThT (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... and normal tissues were mechanically cut into 3-7 mm fragments and incubated in RPMI 1640 medium (Gibco™, cat. 42401042) containing 1 mg/ml Liberase TL Research Grade (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: 500,000 MDA-MB-231 cells plated in a six-well dish were transfected with 20 nM of the appropriate siRNA (IDT) or 20 nM of appropriate LNA diluted in OPTI-MEM containing 7 µL of RNAiMAX (Thermo Fisher). Transfections were performed according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... The qPCR experiments were performed in triplicate in a ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... or Blue ŚGreen qPCR kit (Byozim) on a 7900HT Real Time PCR system or QuantStudio 7 Flex Real-Time-PCR-System (Applied Biosystems). Primer sequence information is provided in supplementary table 4 ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the CSF or plasma SEC fractions 7-10 was then determined using Qubit Protein Assay kit (ThermoFisher Scientific) using 1 μL of sample ...
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bioRxiv - Pathology 2024Quote: ... Gene expression was quantified with an SYBR Green real time-PCR using the ViiA™ 7 system (4453535, Thermo Fisher Scientific). β-Actin was used as a housekeeping gene to normalize the amounts of cDNA in each sample ...
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bioRxiv - Neuroscience 2024Quote: ... were removed from the culture membrane AT 7 DIV with a scalpel blade into pre-filled bead mill tubes (Fisherbrand: 15555799) containing RNAlater stabilisation solution (ThermoFisher: AM7020) and kept at 4°C until RNA extraction ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.