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Citations for Thermo Fisher :
2301 - 2350 of 2674 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (4368708, ThermoFisher Scientific). Primers for HA (5’- AAACTCTTCGCGGTCTTTCCA-3’ sense sequence and 5’-GATAAGGTAGCTTGGGCTGC-3’ antisense sequence) ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 99 °C with shaking for 7 min and 25 μg of protein per sample was analysed with 4-12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... in 25 mM sodium citrate pH 5.0 buffer containing 7 M urea and 10.5 mM EDTA with 1 U RNase T1 (ThermoFisher Scientific, Waltham, MA) at 55°C for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was performed with 2.5 ng of DNA in triplicates using PowerSYBR Green PCR master mix at Quant Studio 7 qPCR machine (both Applied Biosystems) according to the manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... Movies were collected on a Titan Krios G3i at spot size 7 with a Falcon 4 Direct Detector (Thermo Fisher Scientific) equipped with an energy filter set to 20 eV ...
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bioRxiv - Biophysics 2023Quote: ... The cell-culture medium contained a 1:1 mixture of Eagle’s minimal essential medium (EMEM; for MCF-7) and F12 (Invitrogen, Waltham, MA) with a 2% B-27 supplement (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... Proliferation was assessed 7 days after treatment with respective drugs using CellTiter-Glo® luminescent Cell Viability Assay (Fisher Scientific, PRG9242). The luminescence signal was measured in a luminometer ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Cell Biology 2023Quote: ... BAL samples were then centrifuged at 3,000 g for 20 min to collect cells and ApoBDs for staining with a combination of SR-DEVD-FMK (caspase 3/7 activity detection dye, Thermo Fisher), annexin A5-V450 and TO-PRO-3 in 1× A5 binding buffer at room temperature for 10 min ...
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bioRxiv - Genomics 2023Quote: The RT-qPCR reactions were carried out on 384-well plates using a QuantStudio 7 Flex Real- Time PCR System (ThermoFisher #4485701) using 10 µl reactions (2 µl of cDNA 3.7 ng/µl ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... subsequently buffer exchanged by using 7 K MWCO Zeba Spin Desalting Columns and the concentration was measured by Nanodrop One (Thermofisher Scientific).
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bioRxiv - Biophysics 2023Quote: 7 μM Aβ40 was incubated with varying stoichiometries of claramine and 5% PEG (50% w/w 10 kDa, Thermo Fisher Scientific) in the presence of 20 mM ThT (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained at 37°C and 5% CO2 and dissociated using 0.25% trypsin/1 mM EDTA (WISENT 325-043-EL) for 7 min after one wash with 1x DPBS without Ca2+ and Mg2+ (Gibco 14190250) for SCC-13 cells or 3 min for all other cells ...
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bioRxiv - Cell Biology 2024Quote: The mouse macrophage cell line RAW264.7 (ATCC, VA, USA) was cultured in dulbecco’s modified eagle medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, CA, USA) at a 37°C and 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... qRT-PCR was performed using TaqMan Fast Advanced Master Mix and predesigned TaqMan probes on a 384-well plate using the ViiA 7 Rea-time PCR system (Thermo Fisher). The predesigned TaqMan probes used are as follows ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear and cytoplasmic fractions of MCF-7 were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and normal tissues were mechanically cut into 3-7 mm fragments and incubated in RPMI 1640 medium (Gibco™, cat. 42401042) containing 1 mg/ml Liberase TL Research Grade (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 reverse transcription products and standard plasmids were subjected to real-time PCR analysis (QuantStudio 7 Flex Real-Time PCR System; Applied Biosystems) using human β-actin as an internal control ...
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bioRxiv - Bioengineering 2024Quote: ... Formation of spheroids was induced by seeding MCF-7 cells at 750–1250 cells/well in 96-well U-bottomed ultra-low attachment plates (174929, Life Technologies) with shaking ...
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bioRxiv - Biophysics 2024Quote: ... Native PAGE or SDS PAGE gels were transferred to a nitrocellulose membrane using an iBlot2 (20 V, 7 min; Invitrogen™). The membrane was incubated with blocking buffer (PBS ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were then differentiated into macrophages by 7–9 days of culture in IMDM with glutamax base + 10% AB human serum (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... COS-7 cells were transfected with reporter plasmid construct together with pRL-TK Renilla luciferase plasmid by Lipofectamine™ 2000 (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... 2 μl RNA were used in each PCR reaction using KAPA SYBR FAST One kit (KK4651) and run on a QuantStudio 7 Flex System (Applied biosystems).
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bioRxiv - Microbiology 2024Quote: ... the samples were stained with 80 μL CellEvent Caspase 3/7 Green detection Reagent 2 µM in PBS with 5% FBS (Invitrogen, C10423) and then incubated for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: Human embryonic kidney cell line HEK293FT and breast cancer cell line MCF-7 were both cultivated in Roswell Park Memorial Institute medium (RPMI, Thermo Fisher Scientific, Gibco) supplemented with 10% FCS (fetal calf serum ...
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bioRxiv - Cancer Biology 2024Quote: ... dense cultures containing organoids ranging in size from 100 to 500 µm were passaged every 7-10 days with TrypLE (Life Technologies) containing 10 mM ROCK inhibitor and 10 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: Primary cortical neurons were maintained for 7 days (DIV 7) before EV treatment at 37°C in 5% CO2 and cultured in serum-free Neurobasal medium (Life Technologies) supplemented with 1.2% GlutaMAX-I (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... The mRNA expression levels were determined by real-time quantitative RT-PCR using an Applied Biosystems (QuantStudio 7 Flex, Thermofisher, USA). The sequences of specific primers are listed in Supplemental Table 1 ...
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bioRxiv - Plant Biology 2020Quote: The procedure for cloning KDR CDS was as described before but with the use of the primers listed in Supplemental Table 7 and cloned into the Gateway vector pDONR221 (Thermo Fisher Scientific). The competent cells transformed with the entry vectors were selected for growth on the antibiotic kanamycin (50 µg/ml) ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was further used for Real-time quantitative polymerase chain reaction (qPCR) performed on QuantStudio 7 Flex system (Invitrogen™ Life Technologies) using Fast SYBR Green Master Mix (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis was performed by quantitative real-time RT-PCR (qRT-PCR) using SYBR Green PCR Master Mix and the ViiA™ 7 Real-Time PCR System (Applied Biosystems). For each target gene ...
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).
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bioRxiv - Genetics 2021Quote: ... Blocks were later cut on a cryostat and 7-10 μm thick sections were placed on SuperFrost Plus slides (Cat#FT4981GLPLUS; ThermoFisher; Waltham, MA). Hematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Genetics 2021Quote: ... The purified products were separated and detected by capillary electrophoresis (CE) using the ABI 3130 Genetic Analyzer with POP-7 polymer and GeneScan-120 Liz (Thermo Fisher Scientific). The raw data were analyzed using the GeneMapper ID V3.2 software (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... cyli kidneys was determined by quantitative real-time PCR performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific) using the default program ...
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bioRxiv - Molecular Biology 2020Quote: ... was added to bind excess dye and the resultant solution was passed through 7 K MWCO Zeba Spin Desalting Columns (Thermo Fisher Scientific) to remove excess dye ...
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Microbiology 2020Quote: ... The cells were homogenized using an Avestin EmulsiFlex homogenizer and lysed using sonication (programme 7, Fisher Scientific™ Model 705 Sonic Dismembrator) and the resultant lysate was transferred into SS34 tubes for further centrifugation at 18,000 rpm (JA-25.50 rotor ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems QuantStudio Flex 7 Real Time PCR System (Thermo Fisher Scientific). The ΔΔCT method was used for relative quantification of target mRNA expression and normalized to Rn18s (18S rRNA ...
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bioRxiv - Developmental Biology 2020Quote: ... samples from pools of 20 medaka hatchlings (stage 40) and human fibroblasts (7×105 cells) were lysed with RIPA Lysis and Extraction Buffer (Thermo Scientific, #89900). Samples were purified with methanol/chloroform protein extraction protocol according to (Wessel & Flügge ...