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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... cell lysates and virions pelleted through 20% sucrose (14,000xg for 90 minutes at 4°C) were separated on a NuPage 4-12% Bis-Tris Gel (Invitrogen) and subsequently blotted onto a nitrocellulose membrane ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were then applied at 4°C overnight in 1x PBS supplemented with Horse serum (4%, ThermoFisher, 16050114). The following synaptic antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Cell Biology 2019Quote: ... OCT4 and TRA1-60 overnight at 4°C using the pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Life Technologies), followed by incubation with an ALEXA secondary antibodies for 30 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... 4 µl CFE reaction was loaded onto a NuPAGE 4–12% Bis–Tris Gel (Life Technologies, Carlsbad, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated at a density of 4 × 104 cells per well in a 4 well chamber slide (Nunc Lab-Tek II Chamber slide system ...
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bioRxiv - Immunology 2021Quote: Avi-tag-biotinylated spike and RBD were incubated with subsequent fluorochrome-labeled streptavidin at 4:1.5 ratio overnight at 4 °C as follows: spike with APC-streptavidin (Invitrogen), Wuhan RBD with PerCP-Cy5.5-streptavidin (BioLegend) ...
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bioRxiv - Molecular Biology 2021Quote: Protein samples (4×106 cell equivalents per lane) were separated on 4%-12% Bis-Tris gels (Thermo Fisher Scientific) and then transferred to nitrocellulose membranes ...
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bioRxiv - Microbiology 2022Quote: ... or BA.4/5 spike protein (4 μg/mL) was immobilized on 96-well Maxisorp ELISA plates (Thermo Fisher) overnight at 4°C in coating buffer (1X PBS supplemented with 0.05% Tween-20 ...
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bioRxiv - Biophysics 2024Quote: ... 4 µl of cell suspension were applied and plunge-frozen using a Vitrobot Mark 4 system (Thermo Fisher Scientific) at blot force 10 ...
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bioRxiv - Plant Biology 2024Quote: ... for 6h at 4°C and an additional 2h at 4°C with 20μl Dynabeads Protein G (#10003D, ThermoFisher) blocked with 0.1% BSA ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... were treated either with 0.8 μM CF-4 or control CF-4 (Ctrl-S2-CF4) in DMEM without phenol red (Gibco) and incubated for 10 min at 37°C and 5% CO2 ...
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bioRxiv - Biophysics 2023Quote: ... The protein was incubated overnight at 4°C with stirring with 4× molar excess AL594 or AL647 maleimide (Invitrogen). The labeled protein was concentrated and buffer exchanged into 20 mM Tris pH 7.4 ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Immunology 2023Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
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bioRxiv - Bioengineering 2023Quote: ... 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-8) was purchased from ThermoFisher Scientific.
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bioRxiv - Developmental Biology 2024Quote: ... Cells were spun down at 1,000xg for 4 min and fixed with 4% PFA (Thermo Fisher, Cat. No. 28908) for 15 min on ice ...
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bioRxiv - Bioengineering 2024Quote: Live and dead cells were stained with 1.6-4 mM calcein AM (C125400; TRC) and 4 mM ethidium homodimer-1 (EthD-1 L3224; ThermoFisher), respectively ...
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bioRxiv - Biophysics 2021Quote: ... sperm were incubated with 15 μM DEACM-caged cGMP for 7 min in the presence of 0.25% Pluronic™ F-127 (ThermoFisher). Uncaging was performed in the stopped-flow cuvette by a 360 nm LED (Thorlabs ...
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bioRxiv - Biophysics 2021Quote: The proteoliposomes were incubated with buffer IN (100 mM KCl, 20 mM HEPES pH 7, containing either 250 μM Calcein (Invitrogen), 100 μM Fura-2 (ThermoFischer Scientific) ...
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bioRxiv - Biophysics 2020Quote: ... Baculovirus was harvested about 7 days after transfecting bacmid into Sf9 cells cultured in Sf-900 III SFM medium (Gibco) at 27 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Quantitative real-time PCR was performed on a high-productivity real-time quantitative PCR ViiATM 7 system (Life Technologies, U.S.A.) using GoTaq® qPCR Master Mix (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative transcript analysis was performed using a QuantStudio 7 Flex Real-Time PCR System with the Power SYBR Green RNA-to-Ct Real-Time qPCR Kit (all ThermoFisher). Total RNA template (25 ng per reaction ...
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bioRxiv - Immunology 2021Quote: ... total bone marrow cells were isolated from the femur and tibias of mice and differentiated into macrophages for 7 days in complete DMEM medium (500 mL, ThermoFisher) supplemented with M-CSF (40 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from pygidia and several posterior segments of young regenerates (7-10 days of regeneration) of Alitta virens and Platynereis dumerilii using TRIzol reagent (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 ng template was used per reaction with SYBR Green Mastermix in ViiA 7 Real-Time PCR System (Applied Biosystems). As endogenous control ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was then performed for at least 3 biological replicates using Biozym Blue S’Green qPCR Mix Separate ROX (No. 331416XL) on QuantStudio 7 Flex Real-Time PCR System from Applied Biosystems. Pax3 fold change was calculated from ΔCt using Gapdh as housekeeping gene (2^-(ΔCt)) ...
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bioRxiv - Developmental Biology 2021Quote: Micropatterned coverslips with 225-μm diameter synthetic lung buds at day 7 of lung induction were dissociated with TrypLE Express (Gibco) for 10 min at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... was cultured in Dulbecco’ ss Modified Eagle Medium (DMEM) supplemented with 7% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco) at 37°C with 5% CO2.
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bioRxiv - Immunology 2022Quote: ... was used and results were analyzed by quantitative RT-PCR with a Vii 7 Real-time PCR system (Applied Biosystems). The comparative threshold cycle method and internal control (Gapdh ...
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bioRxiv - Developmental Biology 2020Quote: ... We use the following conditions for the 40-cycle reaction on the Quant Studio TM 7 Flex System (Thermo Fisher): a ...
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bioRxiv - Microbiology 2019Quote: ... Samples with RIN > 7 and DNA contamination <10% were used for cDNA synthesis using SuperScript III first-strand synthesis kit (Invitrogen). RT-qPCR was performed using 2xSYBR FAST qPCR universal MasterMix kit (Kappa Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... an in-house generated model of human hepatic Huh-7 cells stably overexpressing mRFP-Rab5 were cultured in DMEM/GlutaMax/High glucose (Gibco) supplemented with 10 % FBS ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
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bioRxiv - Developmental Biology 2019Quote: ... After 7-10d culture cells were shaken in an orbital shaker at 350-400rpm (1.9cm orbital radius, MaxQ 4450, ThermoFisher Scientific) at 37°C to obtain a culture of cortical astrocytes ...
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bioRxiv - Biophysics 2019Quote: 20-nm fluorescent dark red beads samples (Fig. 2d, Supplementary Fig. 5) were prepared using a 5.10−7 dilution of the initial solution (F8783, Thermo Fisher). We performed the dilution in PBS + 5% glucose to match the index of the dSTORM imaging buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... MCF-7 and MDA-MB-231 cells were plated on glass coverslips in serum-free Gibco OptiMEM medium (Life Technologies) and transduced with 4.8×1010 PFU of Ad5-Laconic (Vector Biolabs) ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were transfected at 7-9 days in vitro (DIV) using Lipofectamine 2000 following the manufacturer’s directions (Thermo Fisher Scientific). A total of 1 μg of DNA was transfected for each well of a 12-well plate for 2-3 hours before switching back to conditioned media ...
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bioRxiv - Molecular Biology 2019Quote: Huh-7 (human hepatoma) cells were maintained at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... of WT and Adrb2-/- Cl4 mice (7-12 weeks old) with a negative isolation kit according to the manufacturer’s instructions (Invitrogen #11417D). Donor T cells were derived from CD90.1 congenic animals in order to track their frequency in CD90.2 hosts ...
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bioRxiv - Neuroscience 2020Quote: ... Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1, 3, and 7) with 200 µl Trizol (Invitrogen) per sample and stored at −80 °C until sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...
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bioRxiv - Neuroscience 2020Quote: The pCAG-DCC:TDTOMATO wildtype and missense mutant receptor constructs (0.2 µg) were transfected into COS-7 cells using Lipofectamine® 2000 (Invitrogen). After 48 hours ...