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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and NEAA and insulin as described above for MCF-7 cells and phenol-red free RPMI (Thermo Fisher Scientific), 10% charcoal-stripped fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was changed every 2 to 3 days and the cells were split every 5 to 7 days using 0.25% Trypsin-EDTA (Gibco), up to 20 times ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were obtained by pushing the tissue through a 70 µm cell strainer and subsequently cultured at 7 × 106 cells/ml in stimulation medium consisting of RPMI1640 supplemented with 0.5% penicillin/streptomycin (Gibco), 10% FCS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... HuH-7 cell line was obtained from JCRB Cell Bank and cultured in DMEM (Gibco, catalog no. 31885-023) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... qPCR analysis was carried out in duplicate or triplicate on a Viia 7 real-time PCR system (Thermo Fisher). Fold changes in gene expression relative to either input (0hpi ...
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bioRxiv - Synthetic Biology 2020Quote: COS-7 cells were plated on poly-L-lysine coated coverslips and transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... cDNA quantification was done by real-time PCR on an ViiA 7 real-time PCR system (Thermo Fisher Scientific) using Maxima SYBR Green/ROX qPCR Master Mix (K0222 ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids pCAG-dsRed and Pak3K297R transfection was performed at 7 d in vitro (DIV) with 1 µg of DNA and 1.5 µl of Lipofectamine (Invitrogen), as described in manufacturer’s manual (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... was used for qRT-PCR in a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, USA). Data were analyzed by QuantStudio Software V1.3 (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Embedded samples were cut serially into slices of 7 µm using a MICRO HM355 (Thermo Fisher Scientific, Walldorf, Germany) and stained with Hematoxylin ...
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bioRxiv - Physiology 2020Quote: ... qPCR was run in a 384-well plate format on a ViiA 7 Real-Time PCR System (Applied Biosystems) using 2x SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Physiology 2019Quote: ... 10 μm thickness muscle cryosections were incubated with 5 μM of 2’,7’-dichlorodihydrofluorescein diacetate (DCFH; Molecular Probes, Eugene) and allowed to dry overnight at room temperature in dark ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Liver-Chips were stained in the upper channel with 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA) (Thermo Fisher) to visualize bile canaliculi and MRP2 activity ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... both Huh-7 cells and PHH were processed with the TaqMan Fast Advanced Cells-to-Ct Kit (Invitrogen, A35378), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Bioengineering 2020Quote: ... the cell pellets were incubated for an hour in 2 uM 2’,7’-dichlorodihydrogen-fluorescein diacetate (H2DCF-DA, Invitrogen) in phenol red free media (Sigma ...
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bioRxiv - Immunology 2020Quote: ... 1×106 CAR T cells were co-cultured with γ-irradiated K562-CD19 or K562-CD19-PD-L1 at 1:3 effector:target (E:T) ratio for 7 days and counted with the countess II Automated Cell Counter (ThermoFisher, USA).
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 and Huh-7 cells were transfected with plasmid DNAs by standard calcium phosphate precipitation and Lipofectamine 3000 (Invitrogen), respectively.
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bioRxiv - Microbiology 2020Quote: ... Amplification of the extracted DNA template was performed in QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems) by initial incubation of 2 min at 55°C and 10 min at 95°C ...
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bioRxiv - Cell Biology 2019Quote: ... The plasmids encoding for His-SEPT2 or His-SEPT2-mCherry and SEPT6/7-strep were co-transformed into E.coli BL21 (DE3) (Invitrogen). Bacterial cultures were grown to OD600 of 2-3 and induced with 1 mM IPTG for 1h at 37°C (His-SEPT2 ...
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bioRxiv - Genetics 2020Quote: ... qRT-PCR assays were performed in technical duplicates in 96- or 384-well plates on QuantStudio 7 (Life Technologies) or Bio-Rad CFX 96 instrument ...
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bioRxiv - Genomics 2020Quote: ... These mixtures were measured in triplicate for each sample-primer combination with a ViiA 7 qPCR machine (Thermo Fisher)
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bioRxiv - Molecular Biology 2021Quote: ... Lysosomal ExRai AMPKAR was made by inserting PCR-amplified LAMP149 generated using primers 7-8 into HindIII (ThermoFisher FD0504)/BamHI-digested ExRai AMPKAR backbone ...
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bioRxiv - Microbiology 2020Quote: ... bone marrow derived monocytes cells were incubated for 7 days in presence of RPMI medium-Glutamax (61870-036; Gibco) supplemented with 10% heat-inactivated FBS (16140071 ...
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bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Huh-7 .5 and BHK-21 cells were cultured in Dulbecco’s minimal essential medium (DMEM) (Gibco /Thermo Fisher Scientific) supplemented with 2mM L-glutamine (Gibco /Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: Huh-7 and C6/36 cells were grown in 8 well chambers (LAB-TEK®, Nalgene Nunc International, USA) for 24h ...
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bioRxiv - Immunology 2020Quote: ... Lenti-X 293T and COS-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific, USA) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific, # 89960). Ligand binding affinity and sensitivity were assessed using ELISA techniques.
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bioRxiv - Microbiology 2021Quote: ... All strains were grown on Columbia agar supplemented with 7% (v/v) sheep blood (Oxoid™, Thermo Fisher Scientific) overnight at 37°C under aerobic conditions ...
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bioRxiv - Cell Biology 2022Quote: ... 2007) and DsRed2-Mito (Tanaka Bio) plasmids using a ratio of 7:3 using Lipofectamine LTX Plus (ThermoFisher, 15338100) for 5 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... The formaldehyde supernatant was removed and the pellet was resuspended in 1 mL cold molecular grade 100 mM Tris HCl pH 7 plus 0.1 U/uL SUPERase- In RNase inhibitor (ThermoFisher) to quench the formaldehyde ...
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bioRxiv - Cancer Biology 2022Quote: ... and then cultured on Petri culture dishes for 7 days in Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco 12440-053) supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Biophysics 2022Quote: ... HEK 293 cells and COS-7 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific, USA) supplemented with glucose (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: Cell viability after 1 and 7 days was examined using Calcein AM (Thermo Scientific, Breda, the Netherlands, CCID: 390986) for live cells and propidium iodide (PI ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSC at day 6-7 after passaging were treated with a 1:1 mixture of TrypLE Select (Life Technologies) and 0.5 mM EDTA/phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... in the presence of T cells at a 1:1 effector:target cell ratio in the presence of the caspase-3/7 Green Detection Reagent (Invitrogen). Images were taken every 2 h at 10× magnification ...
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bioRxiv - Microbiology 2022Quote: ... then 7 µg of protein along with PageRuler Prestained ladder 10 kDa to 180 kDa (26616, Thermo Fisher Scientific) was loaded on a NuPage 4-12% gradient Bis-Tris pre-cast SDS-PAGE gel (NP0321 ...
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bioRxiv - Neuroscience 2022Quote: ... of 0.5% low-melting agarose (FMC) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Quantitative RT– PCR was performed using the QuantStudio-6 and -7 Flex Real-Time PCR Systems (Thermo Fisher Scientific) with SYBR® Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Molecular Biology 2022Quote: ... p2×7 (Mm00440582_m1) and tgfβ (Mm01178820_m1) were determined by quantitative PCR using Taqman primers and probe (Applied Biosystems, USA) and Taqman Gene Expression Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ROS levels were detected using the chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) general oxidative stress indicator kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... pH 7.4) in a pre-chilled 7 mm x 20 mm thick-walled polycarbonate ultracentrifuge tubes (Thermo Scientific # 45233) and centrifuged in a S100AT3 rotor at 4°C for 60 min at 50,000 x g (43,000 rpm ...
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bioRxiv - Microbiology 2022Quote: ... using qScript 1-Step SYBR Green qPCR kit (Quantabio) on a ViiA 7 Real-Time PCR System (Applied Biosystems); 16S rRNA was used for normalization ...
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bioRxiv - Biophysics 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-ATTO647N (ATTO-lipid) and (iii) 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-Cyanine 7 (Cy7-lipid) were from Invitrogen, ATTO-TEC and Avanti Polar Lipids ...