Labshake search
Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... thoracic and lumbar DRGs were harvested from 7-to 10-week-old MrgprdGFP mice and transferred to Hank’s buffered saline solution (HBSS) (Invitrogen). Ganglia were treated with collagenase (2 mg/ml ...
-
bioRxiv - Neuroscience 2023Quote: ... SYBR-Green-based real-time quantitative PCR was performed in an ABI QuantStudio 7 Flex machine (Applied Biosystems, USA). Fold-change was calculated by the delta-delta Ct method (Livak & Schmittgen ...
-
bioRxiv - Physiology 2024Quote: ... Approximately 7 µg of tryptic peptide from each tissue sample was labeled with TMT-Pro 16 plex (Thermo Scientific) reagents according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... The Taqman real-time PCR was carried out using Applied Biosystems QuantStudio 7 Flex Real-Time PCR System (Thermofisher), and all the procedures were followed as per the instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... and then cultured on Petri culture dishes for 7 days in Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco 12440-053) supplemented with 10% FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... African green monkey kidney fibroblast-like cell line (COS-7) was maintained in minimal essential medium (MEM, Gibco/BRL) supplemented with 5% (v/v ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation was monitored every 24 or 72 hr for 3-7 consecutive days using CyQuant Reagent (Invitrogen, C35011) by measuring bottom-read fluorescence at 520 nm with excitation wavelength set at 480 nm using EnSight Multimode Plate Reader (PerkinElmer) ...
-
bioRxiv - Genomics 2022Quote: ... mouse pancreatic islets isolated from RipCre-tdTomato mice were trypsinized for 7 minutes with 1 mM of trypsine (Gibco). Dissociated pancreatic cells were directly run into an Influx sorter (Becton Dickinson® ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples were pelleted at 3,000 g, aspirated, resuspended in SYTOX buffer (500 µL 50mM sodium citrate [pH 7], 1 µM SYTOX green (Invitrogen)) ...
-
bioRxiv - Immunology 2022Quote: ... All samples were prepared in triplicate and run on a QuantStudio 6/7 Flex Real-Time PCR System (ThermoFisher). Primer sequences for designated HHV-6 marker (U31 ...
-
bioRxiv - Genetics 2023Quote: ... Quantitative RT-PCR was performed on a ViiA 7 Real-Time PCR System with OptiFlex Optics System (Applied Biosystems) using PowerUp SYBR Green PCR kit (Applied Biosystems) ...
-
bioRxiv - Neuroscience 2023Quote: ... Comparative gene expression of sgsh/CG14291 or hgsnat/CG6903 was determined on a QuantStudio 7 Flex (Thermo Fisher Scientific). Reactions were performed in triplicate in 384-well plates using Platinum SYBR Green qPCR SuperMix-UDG with ROX (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... On day 7 of neural induction cells were replated following the manufacturer’s protocol in a Geltrex-coated (Thermo Scientific) 6-well plate (500x103 cells/well ...
-
bioRxiv - Systems Biology 2023Quote: ... Relative transcript quantifications were done by real-time PCR on an ViiA 7 Real-Time PCR System (Thermo Fisher) using Maxima SYBR Green/ROX qPCR Master Mix (K0222 ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred onto a nitrocellulose membrane using iBlot 2 gel transfer device (7 min at 25 V, ThermoFisher). Membranes were incubated for 1 hour at room temperature (RT ...
-
bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-23,36-39 (Supplementary Methods Table 2 ...
-
bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-59 (Supplementary Methods Table 2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... normalized to the expression of GAPDH on a QuantStudio 6 and 7 Pro real-time PCR system (Applied Biosystems).
-
bioRxiv - Developmental Biology 2023Quote: ... COS-7/ventral spinal cord cocultures were grown for 44-48 hours in motor neuron media (Neurobasal-A (Gibco), 2% B-27 ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher), 200 μM L-ascorbic acid (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... we added 7 µl of the RT mix that contained 0.5 µl of 200 U/µl Superscript IV (Invitrogen), 0.25 µl of 40 U/µl RNAseOUT (ThermoFisher Scientific) ...
-
bioRxiv - Immunology 2023Quote: ... and buffer exchange was performed with a 7 kDa molecular weight cut-off size exclusion resin (Thermo Fisher Scientific) to cryo-EM buffer (10 mM Tris at pH 7.6 and 20 mM NaCl).
-
bioRxiv - Biophysics 2023Quote: ... The reaction mixture was then transferred to 2 ml ZebaSpin MWCO 7 kD desalting column (Thermo Fisher Scientific, USA) to separate the labeled protein from the free dye ...
-
bioRxiv - Cancer Biology 2022Quote: ... 7*103 single cells derived from second-generation spheres were labeled with 1 mM CMFDA (Life Technologies, cat# C7025) for 30 minutes and added on top of the mesothelial monolayer ...
-
bioRxiv - Developmental Biology 2023Quote: ... (7) Mount tissues on glass slides (Superfrost Plus Microscope slides, Fisherbrand) using ProLong Diamond Antifade Mountant containing DAPI (Invitrogen) and store slides at 4µC overnight ...
-
bioRxiv - Biochemistry 2023Quote: ... sample was buffer exchanged into 100 mM ammonium acetate using ZebaTM spin column (7 kDa MWCO, Thermo Fisher Scientific). Sample was loaded into Au/Pd-coated borosilicate emitters (Thermo Scientific ...
-
bioRxiv - Pathology 2023Quote: ... We performed the LN34 and β-actin RT-qPCRs on a QuantStudio 7 Flex (ThermoFisher Scientific, Waltham, MA, USA) as described previously [13 ...
-
bioRxiv - Neuroscience 2023Quote: ... Peptide eluents were separated on a self-packed C18 (1.7 µm Water’s BEH) fused silica column (laser pulled 15 cm × 150μM ID) by Ultimate 3000 RSLCnano (Thermo Scientific). Elution was performed over a 36-min gradient at a rate of 1ul/min with buffer B ranging from 3% to 50% (buffer A ...
-
bioRxiv - Bioengineering 2023Quote: Human breast cancer cell lines MDA-MB-231 (ATCC) and MCF-7 (ATCC) were grown in DMEM/F12 (Gibco) with 15 mM HEPES supplemented with 10% v/v fetal bovine serum (VWR ...
-
bioRxiv - Neuroscience 2023Quote: ... The qPCR analysis was performed with a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific) using the ΔΔCt method [50] ...
-
bioRxiv - Neuroscience 2023Quote: ... The qPCR analysis was performed with a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems; Thermo Fisher Scientific) using the ΔΔCt method [50] ...
-
bioRxiv - Physiology 2023Quote: ... Quantitative real-time PCR was carried out on a ViiA 7 instrument (Thermo Fisher Scientific, Waltham, MA 02451, USA) using HOT FIREPol Probe mix Universal (Solis Biodyne ...
-
bioRxiv - Molecular Biology 2023Quote: ... miRNAs were then quantified using individual TaqMan assays on an ABI QuantStudio 7 real time PCR machine (Applied Biosystems). Assay IDs include ...
-
The effect of single mutations in Zika virus envelope on escape from broadly neutralizing antibodiesbioRxiv - Microbiology 2023Quote: ... the transfection mixture was replaced with low-glucose DMEM supplemented with 7% FBS (Gibco BRI; Life Technologies, Gaithersburg, MD). Cells were then cultured for 4-6 days at 30°C and 5% CO2 ...
-
bioRxiv - Cancer Biology 2023Quote: A549 cells grown in 96-well plates were stained with CellEvent Caspase 3/7 green detection reagent (Thermo Scientific) according to manufacturer’s instructions and the fluorescence measured using a plate reader at 488 nm wavelength ...
-
bioRxiv - Molecular Biology 2024Quote: ... 7 μL for pulldown samples) was mixed 1:1 with 2x TBE-urea sample buffer (Invitrogen, Fisher cat #LC6876), heated to 75°C for 5 min ...
-
bioRxiv - Cell Biology 2024Quote: ... The pellet was resuspended in PBS containing 1:1000 (v/v) 7-AAD viability dye (Invitrogen, catalog number A1310). 500 cells per well were sorted on a BD FACSAria III sorter using a 100-micron nozzle in 4-way purity precision mode into an Eppendorf twin.tec 384 LoBind plate containing 1µl of 0.2% DDM ...
-
bioRxiv - Neuroscience 2024Quote: Transfection of cultured neurons was performed with 300 µl Opti-MEM medium mixed with 7 µl Lipofectamine 2000 (Invitrogen), 1,5 µl Magnetofection CombiMag (OZ Biosciences ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were grown for an additional 7 days before removing beads using a DynaMag-15 magnet (Thermo Fisher Scientific). IL-2 was replenished every 2 days to 40 IU/ml ...
-
bioRxiv - Microbiology 2024Quote: ... Measurements of SYBR fluorescence were performed on ViiA 7 (384-well plates) Real-Time PCR Systems (Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2024Quote: ... supplemented with 7% Fetal Bovine Serum (FBS) (MilliporeSigma, catalog F4135) and 1% Penicillin/Streptomycin (P/S) (Gibco, catalog 15140163) at 37°C with 5% CO2 ...
-
bioRxiv - Plant Biology 2024Quote: Total RNA was isolated from Arabidopsis seedlings 7 days after treatment using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... Real-time qPCR was performed in triplicate with QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Waltham, MA). Cycle threshold (Ct ...
-
bioRxiv - Microbiology 2024Quote: Neutrophils were resuspended in HBSS with Ca2+ and Mg2+ containing 10 μM 2’,7’- dichlorodihydrofluorescein diacetate (DCF) (Molecular Probes) to a final concentration of 1×107 cells/ml and gently agitated for 10 minutes at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... and Fc receptors were blocked with Fc block mAb clone 2.4G2 for 15 minutes at 4°C prior to surface staining for 30 minutes at 4°C with anti-CD4 (clone GK1.5 EF450, Invitrogen) and anti-Vβ3 TCR clone (clone KJ25 ...
-
bioRxiv - Cancer Biology 2022Quote: ... HepG2 cells were fixed with 4% paraformaldehyde and then stored at 4°C in RPMI 1640 (Gibco, Cat# 11875119) media supplemented with 10% heat inactivated FBS ...
-
bioRxiv - Bioengineering 2019Quote: ... The fixed cells were stained with 1 µM 2’-[4-ethoxyphenyl]-5-[4-methyl-1-piperazinyl]-2,5’-bi-1H-benzimidazole trihydrochloride trihydrate (Hoechst 33342, Invitrogen) for 5 minutes ...
-
bioRxiv - Neuroscience 2019Quote: ... post-fixed in 4% paraformaldehyde and 30% sucrose for 4 days before being frozen in Neg50 OCT (Thermo Scientific) 30 micron sections were taken and stored in PBS with sodium azide at 4 °C ...
-
bioRxiv - Bioengineering 2021Quote: ... Stock BODIPY505/515 was prepared by dissolving 4,-Difluoro-1,3,5,7-Tetramethyl-4-Bora-3a,4a-Diaza-s-Indacene (Life Technologies, D3921) powder into dimethyl sulfoxide (DMSO ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were fixed using a 4% PFA solution [0.2M sucrose | 4% paraformaldehyde solution in PBS (Thermo Fisher, J19943-K2)] ...