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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... The medium was changed to 500 μl EDM media 7 days later in 24-well flat-bottom plates for further differentiation (Nunc). After 4-7 days of culturing ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs) were generated for 7 days in respectively IMDM or RPMI-1640 medium (Gibco), supplemented with glutamine ...
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bioRxiv - Plant Biology 2022Quote: ... Entry clones were then introduced into the destination vector pH-Ubi-Cas9-7 which also encodes the Cas9-enzyme (Miao et al., 2013) using LR recombination (Invitrogen). T1 CRISPR/Cas9 lines were genotyped for the absence of the T-DNA construct by using the primers priMR410/411 (Hyg-1F/Hyg-1R) ...
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bioRxiv - Microbiology 2022Quote: ... TcdB protein was diluted to 0.05 µg/µL using phosphate buffer (100 mM KPO4, 150 mM NaCl, pH 7) containing 5X SYPRO Orange (Invitrogen S6650), and a serial dilution of test compound ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Microbiology 2022Quote: ... CellEvent™ Caspase-3/7 Green Detection Reagent (C10723) and PrestoBlue™ Cell Viability Reagent (A13261) were purchased from ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... using either a QuantStudio™ 7 Flex Real-Time PCR System or a StepOnePlus Real-Time PCR System (Applied Biosystems). Individual standard curves were generated specifically for each system ...
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bioRxiv - Microbiology 2022Quote: ... Sydney Strain SS1 (cagA+, vacA s2m2) were grown on brain heart infusion (BHI) agar (Difco, USA) containing 7% heat-inactivated horse serum (Invitrogen), antibiotics and IsoVitaleX as mentioned previously (33) ...
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bioRxiv - Microbiology 2022Quote: ... The loaded plate was amplified for 40 cycles via the ViiA 7 real-time PCR system (Thermo Fisher Scientific, Waltham). For each biological replicates (n = 3) ...
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bioRxiv - Molecular Biology 2023Quote: ... Northern blot analysis of U1 snRNA was performed using 5’-radiolabeled DNA probes (Sequence is listed in Supplemental Table 7) in ultrasensitive hybridization Buffer (Thermofisher). The radioactivity signals were analyzed by PhosphorImager (Typhoon FLA 7000).
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bioRxiv - Plant Biology 2023Quote: ... These cassettes were sub-cloned into a modified binary cas9 vector pH-Ubi-cas9-7 (Fayos et al., 2020; Miao et al., 2013) in a Gateway LR reaction (Invitrogen) to generate p-OsCPR5.1-A and p-OsCPR5.1-C constructs (Fig ...
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resolved by 7% SDS-PAGE and transferred to nitrocellulose membrane by semi-dry i-blot transfer system (IB1001, Invitrogen). Inputs and elution fractions were analyzed on separate gels ...
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bioRxiv - Cancer Biology 2022Quote: The COS-7 cells were seeded on gold-nanoparticle-coated cover glasses and cultured overnight in DMEM (11965-092, Gibco) containing 10% FBS (10082147 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... ABI QuantStudio (model 7 Flex) was used for quantitative PCR reactions with SYBR green master mix (Applied Biosystems; Cat #4367659). The catalog of chosen primers can be found in supplementary data (Supplementary Table 1) ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... RT-qPCR assays were carried out in accordance with the manufacturer’s instructions on the QuantStudioTM 7 Flex RT PCR System (Applied Biosystems), utilizing the indicated amplification cycling conditions ...
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bioRxiv - Physiology 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Developmental Biology 2024Quote: Animals were kept individually in 6-well plates with 7 mL of 0.22 µm FASW and final concentration of 1,000 U/mL penicillin and streptomycin (Fisher Scientific, 15140122). Feeding began 3 days post amputation to allow for the gut to reform ...
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bioRxiv - Molecular Biology 2024Quote: The fluorescence-microscopy based analysis of proliferation described above for the screen was used to validate the effect of the 7 selected miRNA mimics (Dharmacon, ThermoFisher) on HPASMCs (n=4) ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 58°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). For the telomere primers ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 56°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). Each sample was run in duplicate ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s directions on a QuantStudio 7 Flex Real-Time PCR System (Cat. No. 4485701, Applied Biosystems, Waltham, MA). Amplification data and melt curves were analyzed using the QuantStudio Real-Time PCR software v1.7.1 ...
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bioRxiv - Immunology 2024Quote: ... or 96-well flat-bottom plates at a concentration of 7 × 105 – 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco). For both workflows ...
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bioRxiv - Neuroscience 2024Quote: ... was performed using the Applied Biosystems QuantStudio 7 Flex Real-Time PCR system and analysed with the QuantStudio Real-Time PCR Software (v1.7 Thermo Fisher). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... approximately 5 × 105 Huh-7 cells were transfected with 2 μg of purified BAC DNA using Lipofectamine 3,000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was replaced with DMEM + 10% FBS + CellEvent Caspase-3/7 Detection Reagent (Green) according to the manufacturers recommendations (Invitrogen). The plate was then imaged every 15 minutes using phase and GFP channels in the ZEISS Celldiscoverer 7 Automated Live Cell Imager set to 42°C at 5% CO2 and 20% O2 ...
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bioRxiv - Cell Biology 2024Quote: ... 60L°C/60Lseconds), and melt curve (95L°C/15Lseconds, 60L°C/60Lseconds 95L°C/15Lseconds) on a QuantStudio 7 Flex (ThermoFisher). Messenger RNA (mRNA ...
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bioRxiv - Neuroscience 2023Quote: ... The relative levels of mRNA expression were examined utilizing real-time PCR (Applied Biosystems ViiA 7 Real-Time PCR System) with SYBR® GreenER™ reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured for 7 days in Tu 2% media (1205Lu) supplemented with lipid depleted FBS (Omega Scientific, Fisher Scientific). Dox inducible LBR KD was initiated 3 days prior to the experiment ...
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bioRxiv - Immunology 2024Quote: ... The protein was purified with water using a 10 mL 7 kDa MWCO Zeba spin desalting column (Thermo Fisher Scientific), and then by dialysis using a 3.5 kDa MWCO regenerated cellulose Spectra/Por®3 dialysis membrane (Spectrum Labs) ...
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bioRxiv - Immunology 2024Quote: ... BHK-21 cells (7×105/well) were transfected in tissue culture-treated 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) with the LCMV Arm plasmids pCAGGS-NP (0.8 µg) ...
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bioRxiv - Bioengineering 2024Quote: Reactive oxygen species (ROS) were quantitatively and qualitatively determined using the cell permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (ThermoFisher Scientific). H2DCFDA is a type of fluorescein that has been chemically reduced and is used to detect the generation of reactive oxygen intermediates in neutrophils and macrophages ...
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bioRxiv - Biochemistry 2024Quote: ... Liposomes at 5-7 mM were applied to the carbon side of a grid held in a Vitrobot Mark IV (ThermoFisher) chamber kept at 4°C and 100% relative humidity ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
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bioRxiv - Bioengineering 2024Quote: COS-7 cells (ATCC, CRL-1651) and HeLa cells (ATCC, CCL-2) were cultured in high-glucose DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Three technical replicates per reaction was performed on the QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Life Technologies). Data was normalized to housekeeping genes (Tubulin ...
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bioRxiv - Neuroscience 2023Quote: ... Three technical replicates per reaction was performed on the QuantStudio 7 Flex Real-Time PCR system (Applied Biosystems, Life Technologies). Data was normalized to housekeeping genes (Tubulin ...
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR reactions were performed on a QuantStudio 7 Flex PCR machine using PowerUp Sybr Green Master Mix (Applied Biosystems). Corresponding changes in the expression levels of selected genes were calculated using the ΔΔCt method relative to housekeeping genes ...
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bioRxiv - Neuroscience 2022Quote: ... samples were diluted to 1M urea with a blend of 468 μL of 50 mM ammonium bicarbonate (32) and 7 μg of Trypsin (ThermoFisher). The samples were subsequently incubated overnight at 25°C for digestion with trypsin ...
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bioRxiv - Immunology 2023Quote: Lungs were transferred into 7 ml bijoux tubes containing 500 µl digestion mix consisting of 5 mg/ml collagenase I (Gibco) and 10 µg/ml DNase I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Coding sequences were amplified using total RNA from 7 days post-fertilization (dpf) zebrafish using the SuperScript IV One-step RT-PCR kit (ThermoFisher) using the primers listed in Table S1 ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Developmental Biology 2023Quote: ... and Ccnd1 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems), as described above ...
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bioRxiv - Plant Biology 2023Quote: ... 7-day-old seedlings stably expressing LIP1-GFP were vacuum-infiltrated for 30 min with 200 nM MitoTracker Orange (Invitrogen). To test for colocalization seedlings were imaged on a confocal microscope (Zeiss LSM 780 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... iTaq Universal SYBR Green Supermix was used according to the manufacturer’s protocol for RT-qPCR reaction in a QuantStudio 7 Flex (Applied Biosystem by Life Technologies). qRT-PCR primers can be found in Supplementary Information Table1 ...
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bioRxiv - Cancer Biology 2023Quote: MCF-7 and MDA-MB 231 (was a gift from Dr Annette Meeson, Newcastle University) were cultured in DMEM (GIBCO) supplemented with 10% foetal bovine serum (FBS ...