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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... quantitative polymerase chain reaction (qPCR) was performed using a QuantStudio 7 Flex qPCR system (4485701; ThermoFisher Scientific, Waltham, MA, USA) and PowerTrack SYBR Green Master Mix (A46109 ...
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bioRxiv - Biophysics 2022Quote: The proteoliposomes were incubated with buffer IN (100 mM KCl, 20 mM HEPES pH 7, containing either 250 μM Calcein (Invitrogen), 100 μM Fura-2 (ThermoFischer Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... 3.6 x 106 HEK293T17 cells were transfected on a 6-well plate with 7 µl Lipofectamine 3000 (Thermo Fisher Scientific), the packaging plasmids psPax2 (3 µg ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 5-7 days once they reached about 70-80% confluency using TrypLE cell dissociation reagent (Gibco) and gentle scraping before being replated onto new MEFs ...
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bioRxiv - Microbiology 2023Quote: ... Quantitative Real-Time PCR was performed using KAPA SYBR FAST One-Step kit (KAPA) on a QuantStudio 7 Flex (ThermoFisher) with the following cycling conditions ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with Annexin V and propidium iodide using Annexin V apoptosis detection kit (Invitrogen Cat# 50-929-7) as per manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with either 0.1% DMSO or 7 nM bortezomib and 30 min prior to fixation cells were treated with MitoTracker Deep Red (500 nM, Invitrogen). 16 % paraformaldehyde (EMS ...
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bioRxiv - Microbiology 2023Quote: ... 7% of heat-inactivated fetal bovine serum (FBS) and 2% of G-418 disulfate 50mg/mL (all from ThermoFisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... 7 x 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM (# 41965062, Thermo Fisher Scientific) containing 10% fetal bovine serum (#16000-044 ...
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bioRxiv - Molecular Biology 2023Quote: The staining of oxidants was performed with the commercially available 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (#C6827, Thermo Fisher Scientific, USA). Cryo-sections (10 µm ...
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bioRxiv - Immunology 2023Quote: ... Bone Marrow Derived Macrophages were isolated and cultured as previously described (Schappe et al., 2018) and differentiated into mature macrophages over 7 days through culture in BMDM media (Gibco RPMI 1640 ...
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bioRxiv - Immunology 2023Quote: ... Bone Marrow Derived Macrophages were isolated and cultured as previously described (Schappe et al., 2018) and differentiated into mature macrophages over 7 days through culture in BMDM media (Gibco RPMI 1640 ...
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bioRxiv - Physiology 2023Quote: ... qPCR was performed using the QuantStudio™ 7 Flex Real Time PCR System (Applied Biosystems™, New York, NY, USA) and SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Physiology 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Microbiology 2023Quote: The RAW 264.7 macrophage cell line (referred to herein as RAW cells) (ATCC TIB-71) and CD300lf-expressing Huh-7 cells were maintained in DMEM-10 medium (Gibco DMEM medium #11995-065 with 4.5 g/L D-Glucose and 110 mg/L Sodium Pyruvate ...
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bioRxiv - Neuroscience 2023Quote: ... or dominant negative (DN) CaMKII were transfected into COS-7 cells using the Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific). COS-7 cells were lysed in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5 ng/mL IL-7 (Miltenyi, USA) and 12.5 ng/mL IL-15 (Miltenyi, USA) or RPMI 1640 (Gibco, USA) medium supplemented with 100 U/mL IL-2 (Sci-Store ...
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bioRxiv - Neuroscience 2023Quote: ... Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco; UK) on ice ...
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bioRxiv - Microbiology 2023Quote: ... Five to 10 μg of total RNA were separated on a 7% (v/v) polyacrylamide gel (7.3 M urea, 1× TBE) and compared to RiboRuler LowRange RNA ladder (ThermoFisher Scientific). RNA from the gel was then blotted on a nylon membrane (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... and the Applied Biosystems® ViiATM 7 Real-Time PCR System with 384-well Block (Thermo Fisher Scientific; Cat #4453536). We used the comparative Ct (ýýCt ...
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bioRxiv - Biochemistry 2023Quote: ... buffer was exchanged to 200 mM ammonium acetate (pH 4.5) with a Zeba™ 7 K MWCO desalting column (ThermoFisher). After buffer exchange ...
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bioRxiv - Cell Biology 2023Quote: ... Cyclin B14E7E cells were selected for 72 h with Nutlin-3A 5 µM and for 7 days with Geneticin (Gibco) 0.4 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... following manufacturer’s protocols and gene expression quantified via Applied Biosystems Quant Studio 6/7 Real-Time PCR System (Applied Biosystems), for TaqMan (Thermo Fisher Scientific ...
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A gene desert required for regulatory control of pleiotropic Shox2 expression and embryonic survivalbioRxiv - Developmental Biology 2023Quote: ... and on a and on a ViiA 7 Real-Time PCR System using PowerTrack SYBR Green Master Mix (Applied Biosystems) for SV-EnhΔ samples ...
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bioRxiv - Immunology 2023Quote: ... and washed with HBSS and resuspended in appropriate volume of HBSS pH 7.4 supplemented with 5% FBS before staining with 7-aminoactinomycin D (Life Technologies) to exclude dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... was peeled off from the underlying tissue using a wet sterile cotton swab (7) and placed in Opti-MEM medium (Invitrogen) containing Pen-Strep ...
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bioRxiv - Bioengineering 2024Quote: COS-7 cells (ATCC, CRL-1651) and HeLa cells (ATCC, CCL-2) were cultured in high-glucose DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
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bioRxiv - Neuroscience 2023Quote: ... The relative levels of mRNA expression were examined utilizing real-time PCR (Applied Biosystems ViiA 7 Real-Time PCR System) with SYBR® GreenER™ reagent (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: A549 cells encapsulated in hydrogels were stained at the beginning (Day 7) and the end of culture (Day 28) with 2 µM Calcein-AM (Invitrogen) and 30 µg/mL propidium iodide (PI ...
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bioRxiv - Evolutionary Biology 2023Quote: ... gonads were then dissected in 7% MgCl2·H2O on ice and stored in RNAlater (Thermo Fisher Scientific Inc., Waltham, USA), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... scATAC-seq libraries were amplified with 12 and 7 PCR cycles and quantified with the High Sensitivity dsDNA Quanti-iT Assay Kit (ThermoFisher) using a Synergy HTX microplate reader (BioTek ...
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bioRxiv - Immunology 2024Quote: ... The killing capacity of the CTL is represented by the % of target cells undergoing apoptosis after 4hr: the co-culture was stained with CellEvent-Caspase3/7-Green (ThermoFisher) for 30mins ...
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bioRxiv - Plant Biology 2024Quote: ... and one 7 mm surgical cotton ball was placed in each well of the 96 well plates (Thermo Fisher scientific). Black plates were used to minimize light reflection and penetration ...
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bioRxiv - Bioengineering 2024Quote: ... 200 μl of antibody or scFv at 1 mg/ml in PBS was mixed with 10% volume of 1M sodium bicarbonate to adjust pH to 7-8 followed by addition of EZ-Link sulfo-NHS-LC-Biotin (ThermoFisher) to reach a 5:1 biotin:protein molar ratio ...
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bioRxiv - Biophysics 2024Quote: COS-7 and HeLa cells as well as their stable cell lines were cultured in DMEM (Gibco, cat. no. 11965092), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... apoptosis was evaluated in NCI-H1975 or HeLa cells using CellEvent Caspase-3/7 Green ReadyProbe Reagent (Invitrogen, Cat#R37111) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: 1 μg of cDNA mixed with 1μl PLUSTM reagent were transiently transfected into DIV 3-7 cultured neurons by Lipofectamine™ LTX (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was performed for both microRNA and genes using the QuantStudio 7 Pro Real-Time PCR System (Applied Biosystems) and TaqMan Gene Expression Assays (Applied Biosystems ...
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bioRxiv - Bioengineering 2024Quote: ... They were then transferred to 48-well plates (VTC-48, AS ONE Corp., Osaka, Japan) and cultured for 7 days using OncoPro medium (A5701201, Gibco) supplemented with 1% P/S ...
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bioRxiv - Bioengineering 2024Quote: ... 7–35) were routinely grown as described with high glucose (4.5 g/L) and 1% (v:v) GlutaMax (Thermo Fisher Scientific), supplemented with 10% (v:v ...
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bioRxiv - Biochemistry 2024Quote: ... and 17 µL of 1x RNA Sequencing Buffer (20 mM sodium citrate pH 5, 1 mM EDTA, 7 M urea) (Ambion). The mixture was split across three tubes (9/8/8 µL) ...
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bioRxiv - Immunology 2024Quote: ... Each well was aspirated of residual cell culture media and incubated for 7 minutes at 37°C with 1 mL 1X TrypLE (Gibco) to detach the cells from the plate ...
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bioRxiv - Immunology 2024Quote: ... injection at day 0 and day 7 with 20 µg peanut extract in 100 µL PBS mixed with 100 µL Alum (ThermoFisher). In parallel ...
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bioRxiv - Microbiology 2024Quote: Flow cytometric cell cycle analysis of unstimulated and 4HT-treated P3HR1-ZHT cells was performed using pulsed BrdU incorporation (20 min) and nuclear staining with 7-AAD in fixed cells (Invitrogen eBioscience BrdU staining kit ...
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bioRxiv - Plant Biology 2024Quote: Quantification of ROS in guard cells of epidermal peels was measured using 20 µM 2’,7’-dichlorodifluorescein diacetate (H2DCFDA; Invitrogen) according to Pornsiriwong et al ...