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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for 7 ACETOXY 4 BROMOMETHYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was isolated from 7×106 cells using trizol according to the manufacturer’s instructions (Invitrogen - catalogue no. 15596026). Following precipitation ...
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bioRxiv - Cancer Biology 2021Quote: We performed apoptosis assays using the annexin-V FITC/PI kit manual (catalog# P50-929-7; Thermo Fisher Scientific). Cells treated with chemotherapeutics were resuspended in fresh medium and plated in a T-75 flask at 37 °C for 24 h ...
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bioRxiv - Cell Biology 2020Quote: ... then loaded with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethylester (BCECF-AM, 1.6 μM, Life Technologies) for 30 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 2020) and reactions were carried out using a QuantStudio 6 and 7 Flex Real-Time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Ovaries collected from 7-day-old pups were cultured on polycarbonate membrane (Whatman Nucleopore Polycarbonate membrane) in MEM (Gibco) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2019Quote: ... and the hematopoetic stem cells were allowed to differentiate for 7 days in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries were quantified using a ViiA™ 7 Real-Time PCR System (Applied Biosystems Corp., Foster City, CA, USA). Libraries were then pooled and diluted to 4 nM and submitted for sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... were isolated from the embryos and cultured using a Durapore Membrane Filter 0.65 μm (#DVPP02500) placed on a stainless-steel mesh for up to 7 days in Ham’s F-12/DMEM (Gibco) supplemented with Insulin-Transferrin-Sodium Selenite Supplement (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: One hundred and fifty tergal gland segments (Segment 7) and control segments (Segment 6) of Dalotia males were dissected in EBSS (Earle’s Balanced Salt Solution; ThermoFisher) and transferred to into 150 µl ice-cold Schneider’s Drosophila medium and fetal bovine serum (SDM+FBS ...
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bioRxiv - Genomics 2020Quote: ... The purified RNA samples were quantified using Qubit RNA reagent kit on a Qubit fluorometer 3.0 (concentration range 3-7 ng/μl) (Invitrogen). RNA integrity (RINe ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged at 14,000 RPM for 30 seconds and separated on 8% SDS-PAGE gels followed by western blotting with mouse anti-HA (H9658, Clone HA-7, MilliporeSigma) or anti-PGK1 monoclonal antibodies (AB_2532235, 22C5D8, Invitrogen), and secondary polyclonal goat anti-mouse antibodies conjugated to horseradish peroxidase (115–035-146 ...
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bioRxiv - Biochemistry 2022Quote: ... The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Genomics 2020Quote: ... media were replaced with 100 μL full melanoma media without phenol-red containing 4μM Caspase-3/7 activity dye (CellEvent, ThermoFisher). Plates where imaged using a Celigo Imaging Cytometer (Nexcelom ...
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bioRxiv - Cell Biology 2019Quote: ... cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s protocol and visualized using FITC 488 nm filter ...
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bioRxiv - Developmental Biology 2019Quote: ... 7% CO2 in a humidified incubator at 37°C and passaged by dissociation with Accutase (Thermo Fisher Scientific, A1110501) every 3-5 days ...
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bioRxiv - Biochemistry 2019Quote: ... 0.25 M cell equivalents were run on each lane of a 7% NuPage Tris-Acetate gel (Invitrogen, Carlsbad, CA) and then transferred to an Immobilon-FL PVDF membrane (EMD Millipore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... plates were treated with 2 μM of the CellEvent™Caspase-3/7 green detection reagent (Life Technologies, UK) for 60 minutes at 37 °C in the dark ...
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bioRxiv - Physiology 2020Quote: ... Quantitative real-time RT-PCR was performed on a ViiA™ 7 Real-Time PCR System (Applied Biosystems Inc.) The PCR program was ...
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bioRxiv - Neuroscience 2020Quote: ... Transfection of cultured neurons was performed with 300 µl Opti-MEM medium mixed with 7 µl Lipofectamine 2000 (Invitrogen), 1 µl Magnetofection CombiMag (OZ Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... Monocytes adhered to glass coverslips in 12-well plates (1.5×106 cells/well) and were subsequently cultured for 7–9 days in RPMI (Gibco) with 10% FBS (Wisent ...
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bioRxiv - Microbiology 2020Quote: ... swabs were directly incubated with 7 mL of Brain Heart Infusion (BHI) broth (Thermo Scientific Microbiology, Cat. No. CM1135B) at 37°C till turbidity was observed (14-16 h for >95% of samples ...
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bioRxiv - Neuroscience 2020Quote: ... the brains were cut into 7-μm thick coronal sections on a microtome (Microm HM 340 E, Thermo Scientific), and mounted on SuperFrost™ Plus glass slides (Thermo Scientific).
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bioRxiv - Neuroscience 2021Quote: ... Brains were embedded in paraffin and 7 µm-thick sections were cut on a paraffin microtome (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... The number of spheroids was assessed 7 days after plating through an EVOS Cell Imaging System (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... the proteins were subjected to a 7 KDa molecular weight cut-off Zeba Spin Desalting Column (Thermo Fisher Scientific), which was pre-equilibrated with 20 mM HEPES pH7.5 and 150 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa and COS-7 cells were grown in DMEM and RPE1 cells in DMEM/F12 medium (Thermo Fisher Scientific) supplemented with 10% FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Plant Biology 2020Quote: ... Data were collected and analyzed using the QuantStudio™7 Flex Software (Applied Biosystems, Thermo Fisher Scientific, MA, USA). Differential gene expression was quantified based on the △ △Ct method using normalized geo-metric means of the two reference genes (Zm00001d002944 ...
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bioRxiv - Plant Biology 2020Quote: ... Data were collected and analyzed using the QuantStudio™7 Flex Software (Applied Biosystems, Thermo Fisher Scientific, MA, USA). Differential gene expression was quantified based on the △ △Ct method using normalized geo-metric means of the two reference genes (Zm00001d002944 ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... seeded with 2,000 cells/well and stained with Hoescht 33258 (1µg/mL) and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at a dilution of 1000x ...
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bioRxiv - Plant Biology 2021Quote: ... 100 g were transferred into a previously sterilized Magenta GA-7 plant culture box (Thermo Fisher Scientific, Schwerte, Germany), sealed and autoclaved again ...
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bioRxiv - Physiology 2020Quote: ... MCF-10 human mammary epithelial cells and COS-7 fibroblast-like monkey cells were maintained in DMEM (Fisher Scientific) supplemented with 10% fetal bovine serum and 2% penicillin/streptomycin.
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bioRxiv - Immunology 2021Quote: ... biotinylated with 7:1 biotin-to-antibody using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (21435, Thermo Fisher Scientific), was prepared in dilution buffer ...
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bioRxiv - Physiology 2021Quote: ... qPCR was performed on 10ng of cDNA using the SYBR-green method on a QuantStudio 7 Flex (ThermoFisher Scientific) using gene specific primers ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was then applied to a Zeba Spin Desalting Column (7 kDa cut-off, Thermo Fisher Scientific, France) equilibrated in acquisition buffer containing 5% of the detergent concentration used for solubilization and centrifuged 2 min at 1,500 x g and 4°C ...
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bioRxiv - Biophysics 2020Quote: ... and 0.1 mM AMP-PNP) using 0.5-mL Zeba™ spin desalting column (7-kDa MWCO) (Thermo Scientific #89882). AMP-PNP and taxol were added to the flow-through to a final concentration of 1 mM and 10 μM ...
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bioRxiv - Cancer Biology 2022Quote: ... in combination with TaqMan Gene Expression Assays per manufacturer protocol on a ViiA 7 RT-PCR system (Applied Biosystems). The assay IDs for TaqMan Gene Expression Assays used were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were run in a Quant Studio 7 Flex Thermal Cycler (Applied Biosystems, Life Technologies, Eugene, OR, USA). The primer efficiencies were ≥ 95% for all primer pairs ...
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bioRxiv - Neuroscience 2022Quote: ... All reactions were run in a Quant Studio 7 Flex Thermal Cycler (Applied Biosystems, Life Technologies, Eugene, OR, USA). The primer efficiencies were ≥ 95% for all primer pairs ...
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bioRxiv - Cell Biology 2022Quote: ... neurons (5-7 DIV) were transfected with 0.35–1.5 μg of total plasmid DNA using Lipofectamine 2000 Transfection Reagent (ThermoFisher, 11668030) and incubated for 18-24 h.
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bioRxiv - Bioengineering 2022Quote: COS-7 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, high glucose, GlutaMAX Supplement, pyruvate, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Huh-7 (Nakabayashi et al., 1982) and Huh-7.5 (Blight et al., 2002) were cultured in DMEM (Gibco 11965118) supplemented with 10% FBS and non-essential amino acid solution ...
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bioRxiv - Immunology 2022Quote: ... MCF-7 (ATCC HTB-22) breast cancer cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, 11960-051) containing 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher scientific, # 89960). Ligand binding affinity and sensitivity were assessed using ELISA techniques.
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bioRxiv - Cell Biology 2022Quote: ... Coverslips with stained cells were mounted to a glass slide with 7 µl Fluoromount-G (Invitrogen, 00-4958-02). They were sealed with nail polish within 10 minutes after mounting ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking buffer was replaced after one hour with 7 µg/mL mouse anti-occludin antibody (cat# 33-1500, Invitrogen) in PBS with 5% goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher, Foster City, CA, USA) using GoTaq qPCR master mix (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher, Foster City, CA, USA) using GoTaq qPCR master mix (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed with PBS and re-suspended in 0,2 % BSA in Dulbecco’s PBS with Sytox Blue or 7-AAD (LifeTechnologies, Thermo Fisher) to exclude dead cells ...
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bioRxiv - Bioengineering 2020Quote: ... gondii GT1 T NCBI Genome assembly version 7/19/2013 genome references with ERCC sequences (obtained from ThermoFisher website). The raw fastq files from sequencing are aligned to the concatenated genomes with STAR aligner (version 2.6.0c ...