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Citations for Thermo Fisher :
201 - 250 of 919 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... TaqMan Universal PCR Master Mix (Applied Biosystems, Cat. # 4305719) and TaqMan Real-time PCR Assays (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... using TaqMan Fast Universal PCR Master Mix (Life Technologies) and predesigned probes and primers for G6Pase and PEPCK from Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... RNA was then extracted from 100 uL of filtered p2 supernatant and reverse transcribed using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific). Sequences encoding the extracellular domain of the spike protein were initially amplified using KOD Xtreme Hot Start Polymerase ...
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bioRxiv - Neuroscience 2023Quote: ... Primary cultured astrocytes differentiated from NSPCs prepared from E12.5 cerebral cortices or P2 cerebella after being cultured for five divisions in DMEM (Fujifilm Wako) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 1% L-glutamine (Fujifilm Wako) ...
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bioRxiv - Neuroscience 2024Quote: ... Sciatic nerves of 6 mouse pups between postnatal days 2 and 4 (P2 and P4) were collected in L15 medium (Thermofisher, Massachusetts, USA) and incubated for 30 min in 2 mg/mL collagenase II and then 10 min in 0.25 % trypsin containing EDTA at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... The c-Myc epitope was detected with mouse mAb 9E10 (59) or rabbit pAb anti-Myc (Invitrogen PA1981). His6-tagged proteins were recognized with mouse anti-His6 (R&D systems MAB050) ...
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bioRxiv - Cell Biology 2020Quote: ... anti- HA-tag for KRAB protein (1:500, 32-6700 NOVEX HA epitope Tag antibody 5B1D10, Invitrogen, USA) and anti-b-Catenin (1:400 ...
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bioRxiv - Immunology 2021Quote: ... or for intracellular expression using the pYES2/NTC vector (N-terminal Xpress and C-terminal V5 epitope, Invitrogen). The pYD1 system used the yeast internal Apa1/Ap2 system ...
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bioRxiv - Molecular Biology 2022Quote: ... thus leading to a fusion of the emdyp open reading frame with the anti-V5 epitope tag (Invitrogen) and a hexahistidine tag ...
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bioRxiv - Cancer Biology 2019Quote: ... Three 15 amino acid peptides (P1, P3 and P4) and one 14 amino acid peptide (P2) were synthesized (Thermo Fisher Scientific, Rockford, IL). The purity of the peptides was >95% and each peptide was modified by N-terminal acetylation and C-terminal amidation to enhance their stability.
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bioRxiv - Cell Biology 2019Quote: ... with addition of 100 U/ml Universal Nuclease (Thermo Scientific) at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... with addition of 100 U/ml Universal Nuclease (Thermo Scientific) at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... TaqMan Fast Universal PCR Master Mix (Thermo Fisher Scientific, USA) was used ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 μL Pierce™ Universal Nuclease (Thermo Fisher Scientific). Then cells were sonicated for 5 cycles ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μL of TaqMan Universal PCR Master Mix (Applied Biosystems), 1 μL mouse β-actin primer-limited TaqMan Endogenous Control probe (ACTB VIC/MGB) ...
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bioRxiv - Cancer Biology 2020Quote: ... and non-universal primers (Thermofisher Scientific, Sigma-Aldrich or Eurofins) designed using Primer3web online tool ...
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bioRxiv - Cancer Biology 2020Quote: ... Taqman Universal PCR Master Mix (Life Technologies, cat no: 4304437), and StepOnePlus Real-Time PCR system (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: ... and 0.1 U/μL Universal Nuclease (Thermo Fisher Scientific, 88701) and were incubated on ice for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Each reaction contained Taqman universal master mix (PE-Applied Biosystems). After incubation at 50°C for 2 min followed by 95°C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... the TaqMan Advanced Fast Universal PCR Master Mix (Life Technologies), 2 µM of each primer and 1µM of the corresponding UPL probe ...
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bioRxiv - Developmental Biology 2022Quote: ... Universal PCR primer and 0.225ul 100x SybrGreen Dye (Invitrogen, S7563). The test PCR reaction was run in a (type of machine ...
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bioRxiv - Cancer Biology 2022Quote: ... and TaqMan Universal Master Mix (Applied Biosystems, San Francisco, CA) on a QuantStudio 6-flex Real time-PCR instrument (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... using TaqMan Fast Universal PCR Master Mix (4352042, Applied Biosystems) and TaqMan Gene Expression Assays (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2019Quote: ... TaqMan Universal PCR Master Mix II (No UNG, ThermoFisher Scientific) and miRNA-specific TaqMan microRNA Assay was used ...
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bioRxiv - Biochemistry 2020Quote: ... supplemented with 20 U/mL Pierce universal nuclease (ThermoFisher Scientific), and the supernatant cleared by 15 min of centrifugation at 15000 g ...
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bioRxiv - Cancer Biology 2020Quote: ... and SDHB using TaqMan Universal Master Mix II (Thermo Fisher), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... TaqMan Fast Universal PCR Master Mix (Thermo Fisher Scientific 4444965) and Taqman gene expression assays were used for amplification ...
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bioRxiv - Microbiology 2022Quote: ... and the Taqman Universal PCR Master Mix (Applied Biosystems #4324018) was prepared ...
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bioRxiv - Microbiology 2022Quote: ... and the Taqman Universal PCR Master Mix (Applied Biosystems #4324018) was prepared ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 10 μl of Universal PCR Master Mix (PerkinElmer Applied Biosystems) and 7.7 μl of nuclease-free water ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 U/ml Pierce universal nuclease (Thermo Fisher, 88702). Sf9 cells were lysed by sonication and His-USP18 protein was purified by Ni-affinity chromatography ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then gently homogenized using universal nuclease (Pierce/ThermoFisher), resolved by SDS–PAGE electrophoresis and transferred on nitrocellulose ...
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bioRxiv - Neuroscience 2023Quote: ... in 2X Universal Master Mix (Catalog #4324018, Thermo Fisher Scientific). The TaqMan assay was performed in technical replicate for each biological replicate ...
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bioRxiv - Biochemistry 2023Quote: ... and 250 U PierceTM universal nuclease (88700, Thermo Fisher Scientific) were used for tRNA digestion that was carried out for 2 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... using TaqMan Universal PCR Master Mix (Thermo Fisher Scientific #4305719). The following probes were used ...
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bioRxiv - Immunology 2023Quote: ... The TaqMan™ Universal PCR Master Mix (Applied Biosystems™) and TaqMan™ RNA-to-CT™ 1-Step Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 µL TaqMan Universal PCR Master Mix (2×) (Applied Biosystems), and 0.5 µL of each TaqMan Gene Expression Assay reagent ...
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bioRxiv - Molecular Biology 2023Quote: ... TaqMan Universal PCR Master Mix (4304437, Thermo Fisher, Dartford, UK) was used to amplify the region of interest ...
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bioRxiv - Cell Biology 2022Quote: ... and Pierce Universal Nuclease for Cell Lysis (Thermo Fisher Scientific). Lysates were further clarified by centrifugation for 10min at 16000 x g at 4C ...
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bioRxiv - Microbiology 2023Quote: ... or Taqman Fast Universal PCR master mix (Applied Biosystems, 4352042), according to the manufactures’ instructions ...
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bioRxiv - Immunology 2023Quote: ... and 100 units/mL Pierce Universal Nuclease (Thermo Fisher Scientific) and the concentration of protein was measured using a Pierce BCA Assay (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... ABI Universal PCR Master Mix (Applied Biosystems, Foster City, CA), and primer and probe sets purchased from Applied BioSystems ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 40 U/mL Pierce universal nuclease (Thermo Scientific) and cOmplete™ EDTA-free Protease Inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2024Quote: ... using TaqMan Universal PCR Master Mix (Applied Biosystems, UK; 4366072) and predesigned TaqMan probes (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... and 100 units/mL Pierce Universal Nuclease (Thermo Fisher Scientific) and the concentration of protein was measured using a Pierce BCA Assay (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... using TaqMan Universal Master Mix II with UNG (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and using Taqman 2X Universal PCR Master Mix (Applied Biosystems). qPCR was performed on CFX Opus 384 thermal cycler (Bio-Rad) ...
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bioRxiv - Developmental Biology 2024Quote: ... and blocked with CAS universal blocking solution (Thermo Fisher, 008120) for 30 min at room temperature to reduce nonspecific binding ...
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bioRxiv - Cell Biology 2020Quote: ... Human PAR4 containing an N-terminal V5-epitope were stably expressed using the HEK293 Flp-In T-Rex cells according to the manufacturer’s protocol (Invitrogen). The concentration of tetracycline was titrated (700 ng/mL ...
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bioRxiv - Genetics 2020Quote: ... To generate donor plasmids the desired homology arm’s regions and epitope tag (either V5 or FLAG) were synthetized by Invitrogen GeneArt Gene Synthesis and cloned into the vector backbone pMK-RQ ...