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Citations for Thermo Fisher :
401 - 450 of 919 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... qPCR reactions were with Taqman Fast Universal PCR Master Mix (4366072, Applied Biosystems) in duplicate using 5 ng of cDNA for template each ...
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bioRxiv - Genetics 2021Quote: ... using Universal PCR Master Mix and TaqMan Gene Expression Assays (Thermo Fisher Scientific). SDS 2.2.1 software was used to extract raw data ...
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bioRxiv - Immunology 2021Quote: ... Bulk mRNA was analyzed by qPCR using TaqMan Universal PCR Master Mix (ThermoFisher).
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bioRxiv - Immunology 2021Quote: ... Mtb genomes were then quantified using Taqman Universal Master Mix II (Life Technologies) and previously published sigF primer-probe combination (Lin et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Each 10μl qPCR reaction contained 1x TaqMan Fast Universal Master Mix (ThermoFisher, 4352042), 100nM of forward primer ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed with TaqMan universal PCR master mix (Thermo Fisher Scientific, 4304437) on the Applied Biosystems Quant Studio 3 system ...
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bioRxiv - Microbiology 2022Quote: ... and TaqMan assays (TaqMan Universal PCR master mix, no AmpErase UNG; Applied Biosystems) were performed using the ABI StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Immunology 2022Quote: ... Duplex Quantitative PCR was performed using the TaqMan Universal PCR Mastermix (Applied Biosystems) to amplify IL15 gene and 18SrRNA housekeeping gene ...
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bioRxiv - Physiology 2022Quote: ... and qPCR was conducted with the Syber GreenER qPCR Super Mix Universal (Invitrogen), in the StepOne Plus equipment (Applied Biosystetems - Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... a mastermix was prepared by combining 2xTaqMan Universal Mastermix Fast (Life Technologies #4444965) with 10 µM ND1 Primers F+R ...
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bioRxiv - Microbiology 2023Quote: ... and 1 U/mL Pierce™ Universal Nuclease (Thermo Fisher; Waltham, Massachusetts, USA). Lysates were stored at −20°C ...
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bioRxiv - Neuroscience 2023Quote: ... qPCR was performed with SYBR Green Universal Master Mix (Applied Biosystems, cat. # 2107118) in Stratagene Mx3005 real-time PCR machine ...
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bioRxiv - Molecular Biology 2023Quote: ... Free nucleic acids were removed using Pierce Universal Nuclease (Thermo Fisher Scientific, USA). Samples were concentrated using a Pierce ™ PES protein concentrator (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed using either Taqman Fast Universal PCR Master Mix (Applied Biosystems) assays or iTaq Universal SYBR-Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... the same reagents were used with Taqman Fast Universal PCR Mastermix (Applied Biosystems) and the same cycling except for the 50°C cDNA synthesis step ...
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bioRxiv - Neuroscience 2023Quote: ... with 12μL of master mix (TaqMan Universal Master mix fast, Life Technologies #4444964) and 8μL of lysate ...
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bioRxiv - Bioengineering 2024Quote: ... Taqman universal master mix and Taqman probes (Thermo Fisher Scientific; Waltham, MA, USA) were used for amplification and detection of specific expression of Col1a1 ...
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bioRxiv - Molecular Biology 2024Quote: ... RELA (#Hs01042019_g1) and TaqMan® Fast Universal PCR Master Mix (Applied Biosystems; #4352042). All PCRs were performed as duplicate reactions on an ABI7500 Fast real time PCR instrument ...
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bioRxiv - Cell Biology 2024Quote: ... DNA content was digested using 1250U of Pierce™ Universal Nuclease (Thermo Scientific) for 2 hrs at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... were used in conjunction with Taqman Universal Master Mix II (Applied Biosystems; #4440038) for quantitative reverse transcription PCR analysis on the StepOne/QuantStudio 6 Flex Real Time PCR Systems (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... The c-Myc epitope was detected with mouse mAb 9E10 (diluted 1:1000) (55) or rabbit pAb anti-Myc (diluted 1:1000) (Invitrogen, catalog no. PA1981). The V5 epitope was detected with mouse mAb anti-V5 (diluted 1:1000 ...
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bioRxiv - Immunology 2021Quote: Cells were stimulated with 2 μg/mL of MTB300 epitope peptide pool (A&A Labs, USA) in the presence of brefeldin A and monensin (Thermo Fisher Scientific, USA) for 6 hours at 37°C in Complete media (RPMI-1640 with 10% fetal calf serum (FCS) ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently into the F279-V5 mammalian expression destination vector (a Gateway™ compatible plasmid which expresses inserted genes from a constitutive CMV promoter and has suppressor read through capabilities to incorporate a V5 epitope at the C-terminus)36 using LR Clonase™ II enzyme mix (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: ... Paraffin sections of all tumors and normal skin were deparaffinized and antigen retrieval was performed using heat induced epitope retrieval method (HIER) with 1X Antigen Retrieval solution (Invitrogen #00-4955-58) in a pressure cooker at 95 for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... BMP6 was a generous gift from OSTEOGROW. ERFE was detected using DYKDDDDK Epitope Tag HRP-conjugated Antibody (R&D, Cat. # HAM85291) and FAM132B Polyclonal Antibody (Invitrogen, Cat. #PA5-67448). Recombinant ALK3-Fc fusion protein was purchased from R&D (Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... 2008) N-terminally tagged with the V5 epitope was synthesized as a DNA string (GeneArt Strings DNA Fragments, Thermo Fisher Scientific, Schwerte, Germany) and cloned into pcDNA3.1 to obtain pcDNA3.1-V5-RHEB-Q64L ...
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bioRxiv - Cell Biology 2020Quote: ... Real-time polymerase chain reactions were performed with TaqMan Universal PCR MasterMix (Applied Biosystems) or SYBR Green MasterMix (Fermentas ...
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bioRxiv - Cell Biology 2020Quote: ... Pre-designed probe sets and TaqMan universal PCR Master Mix (2x, Thermo Fisher Scientific) were used as follows ...
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bioRxiv - Microbiology 2019Quote: ... cDNA was quantified using TaqMan Universal Master Mix 2 (Applied Biosystems, Waltham, MA, USA). qRT-PCR was performed on BioRad CFX connect real-time system (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... 10 μL TaqMan® Universal PCR Master Mix (Applied Biosystems, Branchburg, New Jersey, USA), 0.25 μL of each of the two matched primers (one forward and one reverse primer for longer amplicon or one forward and one reverse primer for shorter amplicon ...
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bioRxiv - Developmental Biology 2019Quote: ... Real-time PCR reactions were performed using PowerSYBR Green Universal Master Mix (Thermo Scientific) and analysed using ViiA7 Real Time PCR system (Applied Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... We used the TaqMan Universal PCR Master Mix with No AMPErase UNG (Applied Biosystems) and 1μl of cDNA in a total volume of 20 μl ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with Fast Start Universal SYBR Green Master Mix (Applied Biosystems). Relative gene expression levels were calculated using the formula 2 −CtΔΔ ...
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bioRxiv - Systems Biology 2021Quote: ... qRT-PCR reactions were performed using Universal PCR master mix (Applied Biosystems, Branchburg, NJ) and TaqMan gene expression assays (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Second DNA amplification round was performed with TaqMan™ Universal PCR Master Mix (ThermoFisher) using the following primers ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was prepared with 2x TaqMan Fast Universal PCR MasterMix (cat. num. 4366072, ThermoFisher) and TaqMan probes for Axin2 (Mm00443610_m1 ...
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bioRxiv - Biochemistry 2022Quote: ... RT-qPCR was performed using TaqMan Fast Universal PCR Master Mix (4,352,042, Applied Biosystems) and TaqMan Gene Expression Assays (the primers ID ...
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bioRxiv - Immunology 2021Quote: ... Amplification was performed using TaqMan Universal PCR Master Mix (ThermoFisher Scientific, Cat. No. 4324018) and analyzed using the ΔΔCt method37.
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bioRxiv - Cell Biology 2022Quote: ... 40 ng of cDNA were mixed with 2× Taqman Universal Master Mix (Applied Biosystems) and 20× Taqman Gene Expression assay probes (Ripk1 ...
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bioRxiv - Developmental Biology 2022Quote: ... and cDNA was amplified and detected using TaqMan Universal PCR master mix (Applied Biosystems) and TaqMan probes ...
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bioRxiv - Immunology 2020Quote: ... as well as TaqMan Fast Universal PCR Master Mix (Applied Biosystems/ Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Immunology 2020Quote: ... as well as TaqMan Fast Universal PCR Master Mix (Applied Biosystems/ Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Developmental Biology 2021Quote: ... 1⍰µl of cDNA was amplified with the Taqman Universal Mastermix II (Life Technologies), in a 10⍰µl total reaction volume ...
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bioRxiv - Neuroscience 2021Quote: ... with 1 mg/mL lysozyme and 20 U/mL Pierce Universal Nuclease (Thermo Fisher) and subsequently centrifuged ...
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bioRxiv - Biochemistry 2020Quote: ... RT-qPCR was performed using TaqMan Universal PCR mastermix 2x (Applied Biosystems, ThermoFischer Scientific) supplemented with AmpErase UNG (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then prepared with TaqMan Fast Universal PCR Master Mix (Life Technologies PN435042) and loaded on one end of the array ...
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bioRxiv - Microbiology 2021Quote: ... followed by RT-qPCR with TaqMan universal PCR master mix (ThermoFisher cat no. 4305719) and TaqMan gene expression assay probes (ThermoFisher GAPDH cat no ...
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bioRxiv - Microbiology 2022Quote: ... 25 µL of TaqMan Universal Master Mix II with UNG (Thermo Fisher, cat#4440038), and 12.5 µL water ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time PCR was performed with TaqMan™ Universal PCR Master Mix (Applied Biosystems) on a Bio-Rad CFX96/384 thermocycler ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was extracted using the MagVetTM Universal Isolation Kit (Thermo Fisher Scientific, UK) and the KingFisherTM Flex (Thermo Fisher Scientific ...