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Citations for Thermo Fisher :
101 - 150 of 919 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and Pierce Universal Nuclease (Thermo Fisher Scientific). After 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Taqman Universal Mastermix II with UNG (ThermoFisher), NEBNext Small RNA Library Prep kit (NEB) ...
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bioRxiv - Neuroscience 2021Quote: Freshly dissected P2 retinas were homogenized and cross-linked for 10 minutes using 1% formaldehyde (ThermoFisher Scientific Cat# 28906) on a tube rotator at room temperature ...
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bioRxiv - Biophysics 2020Quote: Recombinant GST-tagged PH-domain of PLCd detecting the membrane lipid PI(4,5)P2 was produced and conjugated to of amine-reactive Alexa Fluor 647 carboxylic acid succinimidylester (Invitrogen) as previously described49 ...
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bioRxiv - Microbiology 2021Quote: ... 6.25 µl of 4 μM primers P1 and P2 and 1 µl of template DNA (Thermo Scientific, cat#F553S), and milliQ water up to 50 μl ...
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bioRxiv - Biophysics 2021Quote: ... P2 viruses (8%) were used to infect tsA201 cells grown in Freestyle 293 Expression Medium in suspension culture (ThermoFisher). Infected cells were incubated for an initial 12 h at 37 °C before 10 mM sodium butyrate was added ...
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bioRxiv - Molecular Biology 2022Quote: ... p2×7 (Mm00440582_m1) and tgfβ (Mm01178820_m1) were determined by quantitative PCR using Taqman primers and probe (Applied Biosystems, USA) and Taqman Gene Expression Master Mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... Pooled virus from 2 wells of supernatant (P1 stock) was used to produce high titre P2 stock by transfection of 1.5x106 Sf9 cells/mL (Thermofisher) in 450mL using Insect-XPRESS with L-Glutamine (Lonza LZBE12-730Q) ...
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bioRxiv - Plant Biology 2024Quote: ... Coding sequences of the proteins to be analyzed were cloned into pDONR221 P1-P4 and pDONR221 P3-P2 (Invitrogen) and used for LR reaction with the destination vector pBiFCt2in1-NN (for NRL5 or RPT2 interaction with RABs and VAMP721 or NRL5/RPT2 self-interaction ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-6x-His epitope tag monoclonal antibody (Thermo Fisher Scientific, Cat. No. MA1-21315); LI-COR IRDye 800CW goat anti-rabbit IgG (LI-COR ...
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bioRxiv - Immunology 2020Quote: ... or for a conformational epitope of GFP to detect correctly folded GFP (clone 3E6, Invitrogen).
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bioRxiv - Cell Biology 2021Quote: ... The cDNAs were ligated to the appropriate epitope sequences and cloned into the pcDNA3.1 (Invitrogen) and pGEX-6P-1 (GE Healthcare ...
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bioRxiv - Genetics 2022Quote: ... and the V5 epitope was detected using mouse anti-V5 antibody (Invitrogen R96025, 1:5,000) followed by goat anti-mouse IgG-HRP (Jackson ImmunoResearch 115-035-003 ...
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bioRxiv - Cell Biology 2022Quote: ... and goat anti-mouse with epitope IgG2a Alexa Fluor 633 for CD81 (Invitrogen ref: A21136). For the experiments showing the actin cytoskeleton ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified fragments were then cloned into the corresponding pDONR221 vector (pDONR221 P5–P4, P4r–P3r, and P3–P2) by Gateway BP reactions (Invitrogen) to generate entry clones suitable for MultiSite Gateway LR cloning ...
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bioRxiv - Microbiology 2020Quote: ... The N- and C-terminus of thyA (lpg2868) were amplified by BKMP94-95 and BKMP98-99 primers and introduced into pDonorP1-P4 and pDonorP3-P2 (Invitrogen) to generate pMME1084 and pMME1094 ...
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bioRxiv - Microbiology 2021Quote: ... On day 3 the cells were transfected with 60 pMol of either miR-122 or control microRNA (miR-122 p2-8) or anti-miR-122 LNA or control LNA (anti-miR-124) with 5uL of Lipofectamine 2000 (Invitrogen). Cells were harvested 2 days post-transfection and luciferase activity was measured.
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bioRxiv - Microbiology 2020Quote: The reconstructed ASFLI-A104R gene (Supplementary Figure 2 N2/P2) was custom-synthesised and inserted into vector pMA-T (Geneart, Invitrogen). It was then amplified by PCR and the products were inserted into vector pMAL-p2X (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... carrying either attP1-P4 or attP3-P2 recombination sites using the BP Clonase™ II enzyme mix (Thermo Fisher Scientific). To generate pDONR221 entry clones carrying the TRB4 gene ...
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bioRxiv - Plant Biology 2023Quote: ... and recombined into either the Gateway DONR vectors pBSDONR(P1-P4) or pBSDONR(P4r-P2) using BP Clonase II (Invitrogen) (Carter et al. ...
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bioRxiv - Neuroscience 2024Quote: ... hippocampi from WT and App KI pups (P0-P2) were isolated in ice-cold HBSS (1 mM sodium pyruvate, 1% HEPES; Gibco) and trypsinised (0.25% trypsin ...
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bioRxiv - Immunology 2019Quote: ... and TaqMan Fast Universal PCR Master Mix (ThermoFisher) in a CFX384 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Universal primers and BigDye Terminator Chemistry (Applied Biosystems) were used for Sanger sequencing randomly selected plasmid subclones to a depth of 10x ...
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bioRxiv - Cancer Biology 2020Quote: ... with Taqman Universal Master Mix II (Life Technologies). Primer sequences and Taqman probe IDs are described in Supplemental Table 4 ...
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bioRxiv - Cancer Biology 2020Quote: ... TaqMan Universal PCR Master Mix (Applied Biosystems-ThermoFisher) and PrimeTime standard qPCR primer/probe sets from Integrated DNA Technologies (Iowa City ...
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bioRxiv - Cancer Biology 2020Quote: ... TaqMan Universal PCR Master Mix (Applied Biosystems-ThermoFisher) and PrimeTime standard qPCR primer/probe sets from Integrated DNA Technologies (Iowa City ...
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bioRxiv - Biophysics 2021Quote: ... and 25 U/ml Pierce universal nuclease (Invitrogen). The crude lysate was sonicated on ice and clarified by centrifugation for 30 min at 4°C and 35,000 rpm in a Beckman L90K Optima and 50.2 Ti rotor (Beckman ...
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bioRxiv - Cell Biology 2021Quote: ... or Taqman Universal PCR Master Mix (Life Technologies) on either a Viia7 thermocycler (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... using TaqMan Universal PCR master mix (Applied Biosystems) and pre-designed TaqMan probes (TaqMan Gene Expression Assays ...
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bioRxiv - Neuroscience 2021Quote: ... Universal PCR Master Mix II (Life technologies, #4440038) and ViiA7 Real Time PCR system (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... TaqMan Fast Universal Master Mix 2X (Applied Biosystems) was performed in a total volume of 10uL per well with 0.5uL of each probe and approximately 10ng of template cDNA ...
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bioRxiv - Physiology 2022Quote: ... using Taqman Universal Master Mix II (4440040; ThermoFisher) and Taqman probes (all from Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The EXPRESS qPCR SuperMix Universal Kit (Invitrogen, UK) was used for real time-PCR and the PCR reactions for FMDV 3D were performed as previously described ...
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bioRxiv - Developmental Biology 2019Quote: ... using the Taqman Universal Master mix (Applied Biosystems). The following probes were used ...
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bioRxiv - Immunology 2019Quote: ... and 5µl TaqMan Universal PCR Mastermix (Applied Biosystems) was added to each well ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... using Universal TaqMan MasterMix (2x concentrated, Life Technologies) and TaqMan assay (20x concentrated ...
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bioRxiv - Immunology 2020Quote: ... and amplified using TaqMan Universal PCR Mastermix (ThermoFisher) with 9 μM of forward primer (5-GCGAGAAAGTTAAAAGAATTGA-3) ...
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bioRxiv - Immunology 2020Quote: ... 5 μl TaqMan Fast Universal Master Mix (ThermoFisher), and 0.5 μl 6-carboxyfluorescein (FAM)-labeled TaqMan probes and primers (TaqMan Gene Expression Assays ...
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bioRxiv - Physiology 2023Quote: ... Platinum Direct PCR Universal Master Mix (ThermoFisher, A44647500) was used for DNA isolation and PCR ...
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bioRxiv - Physiology 2022Quote: ... using TaqMan Universal PCR master mix (Applied Biosystems) and pre-designed TaqMan probes (TaqMan Gene Expression Assays ...
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bioRxiv - Immunology 2022Quote: ... and Universal PCR Master Mix (Applied Biosystems™) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... using TaqMan Fast Universal PCR Mastermix (Applied Biosystems) and compared with hypoxanthine phosphoribosyltransferase 1 (Hprt1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and TaqMan Universal PCR Master Mix (Applied Biosystems). The specifically designed primers and the product numbers of the TaqMan Gene Expression Assay were listed in Supplementary Table S5 ...
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bioRxiv - Cell Biology 2023Quote: ... 20 U/ml Pierce Universal Nuclease (Thermo Scientific), 50 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... with Taqman Universal PCR Master Mix (Thermo Fisher) and pre-designed Taqman probes targeting 18S (Hs99999901_s1) ...
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bioRxiv - Developmental Biology 2023Quote: ... using the TaqMan Universal Master mix (Applied Biosystems). The following probes were used ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Alu-qPCR was performed using 10ng of extracted DNA in triplicate using amplification protocol by Applied biosystems (Universal Master Mix II, no UNG: Applied Biosystems Cat# 4440040 and Alu Probe ...
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bioRxiv - Cell Biology 2022Quote: ... or TaqMan Universal PCR Master Mix (Applied Biosystems). All assays were performed in duplicate ...
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bioRxiv - Genetics 2022Quote: ... using TaqMan Universal PCR Master Mix (Applied Biosystems) with two technical replicates per reaction ...
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bioRxiv - Plant Biology 2023Quote: ... The QuantumRNA™ Universal 18S Internal Standard (Invitrogen) was used as an internal control.