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Citations for Thermo Fisher :
301 - 350 of 919 citations for Universal TT epitope P2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and 1 μL/mL Pierce Universal Nuclease (Thermo Fisher Scientific, 88700), and incubated at 4C for 1H ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were treated with 2000 units Pierce universal nuclease (Thermo Scientific) for 45 minutes at RT before increasing the salt concentration to 300 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.1% (v/v) Pierce universal nuclease (88700, Thermo Fisher Scientific). Lysates were centrifuged at 13,000 ×g for 5 min and the supernatant was kept on ice for immediate use or stored at -20°C ...
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bioRxiv - Immunology 2024Quote: ... and nuclease (Pierce™ Universal Nuclease for Cell Lysis; Thermo Scientific). Aliquots normalized to 65 µg protein (EZQ™ Protein Quantitation Kit ...
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bioRxiv - Cell Biology 2024Quote: ... 10 U/ml universal nuclease (Thermo Fisher Scientific, Waltham, MA, USA) and 1x complete protease inhibitor cocktail (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... Epitope confirmation was done by a competitive binding assay where saturating concentration of ICR-10 PE antibody (Thermo Fisher Scientific) was coincubated with a concentration gradient of unlabeled E1-1DD or E4-1DD ...
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bioRxiv - Bioengineering 2019Quote: Paraffin-embedded aorta sections mounted slides were subjected to heat-induced antigen epitope retrieval with citrate buffer (Thermo Scientific, MA). The slides were incubated overnight at 4°C with primary antibodies for anti -MMP-9 (Rabbit anti-Mouse ...
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bioRxiv - Molecular Biology 2021Quote: ... The proteins were then transferred to polyvinylidene difluoride (PVDF) membranes (Immobilion) and the membranes probed with primary antibodies (anti-V5 epitope (Invitrogen), anti-HA epitope (Abcam) ...
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bioRxiv - Microbiology 2021Quote: ... and the titer of the antibody against the epitope was detected using an enzyme-linked immunosorbent assay (cat. no., 44-2404-21, Nunc MaxiSorp flat bottom ...
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bioRxiv - Microbiology 2020Quote: ... fused to a Gly-Ser-Gly linker and a Myc epitope tag into the EcoRV site of pEF1 V5 His C (Invitrogen) using NEB HiFi Assembly Mix (New England Biolabs ...
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bioRxiv - Immunology 2019Quote: ... when epitope-specific anti-PR3 moAbs were used to immobilize iHm5-Val103 on Maxisorp® plates from Invitrogen (Carlsbad, CA), complexation of moANCA518 with the antigen was detected after incubation of HRP-conjugated anti-human IgG antibody (1:250 dilution ...
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bioRxiv - Microbiology 2023Quote: ... and the OLLAS tag was detected using the rat mAb anti-OLLAS.72 The c-Myc epitope was detected with mouse anti-Myc (mAb 9E10) or rabbit polyclonal antibody (pAb) (#PA1981; Invitrogen). Toxoplasma-specific antibodies include rabbit pAb anti-IMC673 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue was manually processed with 20 minutes heat-induced epitope retrieval at pH 9 followed by 15 minutes of proteinase K (1μg/ml; Life Technologies) digestion at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... The cell suspension was transferred to a 96-well V-bottom plate in duplicate and fluorescently labeled with the anti-FLAG epitope tag antibody and a goat anti- mouse IgG secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: MAD1 was tagged at the C-terminus with a 3xHA-Flag epitope by PCR and cloned into a modified version of pcDNA5 FRT/TO (ThermoFisher Scientific). Full length MAD1 carrying either L51G/E52A/E53A/R54A/E56 or E53Q/E56Q mutations were tagged at the C-terminus with mRuby by sub-cloning into the pMCSV vector ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysis and processing for co-immunoprecipitation were done as described with the PierceTM HA Epitope Antibody Agarose conjugate (Thermo scientific).
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bioRxiv - Microbiology 2021Quote: ... All constructs have a C-terminal 3XFLAG epitope tag and were cloned into the pcDNA4/TO vector backbone (Thermo Fisher, #V102020) using restriction sites at EcoRI/XhoI.
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bioRxiv - Neuroscience 2023Quote: ... Epitopes were unmasked using pretreatment with 1.3 µg/ml proteinase K for 10-15 min at 37 °C (Invitrogen, Darmstadt, Germany). After blocking of unspecific binding sites with bovine serum albumin (BSA) ...
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bioRxiv - Genetics 2023Quote: ... The presence of recombinant CENH3 proteins on the membrane was always verified by detection with the HisG epitope tag antibody (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were processed through de-waxing and antigen retrieval in citrate buffer at pH 6.0 using a heat-induced epitope retrieval PT module (Thermo Fisher Scientific) before proceeding with immunostaining ...
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bioRxiv - Molecular Biology 2024Quote: In pcDNA3.1-TRIP6-HA the mouse Trip6 CDS with a C-terminal HA epitope was cloned by PCR between the NheI and EcoRI sites of pcDNA3.1 (Invitrogen, Karlsruhe, Germany). The KpnI / XhoI fragment of pcDNA3.1-TRIP6-HA ...
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bioRxiv - Bioengineering 2024Quote: Antigenic preservation of prefusion epitopes was evaluated by bio-layer interferometry (BLI) using the prefusion-specific antibodies D25 (46, 47) (Thermo Fisher), AM14 (47 ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μg monoclonal anti-Syp antibody directed against a cytoplasmic epitope (cat no 101011, synaptic systems, Germany) or control mouse IgG (10400C, Thermofisher Scientific) was used to incubate with 20-30 μl beads for 30 mins at RT in 2% BSA dissolved in IP buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed with the TaqMan Fast Universal PCR Master Mix (ThermoFisher) and the TaqMan probes GAPDH (4352339E ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1 % sodium dodecyl sulfate (SDS) and Pierce Universal Nuclease (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Genomics 2020Quote: ... qPCR reactions were performed with TaqMan Universal PCR Master Mix (Thermo Fisher) using probes for Tyr (Mm00495817_m1) ...
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bioRxiv - Cancer Biology 2019Quote: ... Resulting cDNA was analysed using TaqMan Universal Master Mix II (Life technologies). Gene expression was determined using an Applied Biosystems 7300 Real-Time PCR system by the comparative ΔΔCT method ...
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bioRxiv - Developmental Biology 2019Quote: ... Pre-amplified samples were combined with TaqMan Universal Master Mix (Life Technologies) and 20x GE sample loading reagent (Fluidigm) ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR (qPCR) was achieved with Taqman Universal Master Mix (Applied Biosystems) with the following commercially bought Taqman Assays ...
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bioRxiv - Physiology 2021Quote: ... Specific primers and TagMan Universal Master Mixer were purchased from Applied Biosystems. TagMan Reaction Mixes contained 3 μl cDNA (10 ng/μl) ...
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bioRxiv - Bioengineering 2020Quote: ... Real-time PCR was performed using TaqMan Universal Master Mix II (ThermoFisher) along with the following TaqMan primers ...
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bioRxiv - Neuroscience 2021Quote: Quantitative PCR was run with TaqMan Universal Master Mix (ThermoFisher catalog #4440042) and TaqMan FAM-MGB primer/probe sets spanning exon junctions on a BioRad CFX96 ...
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bioRxiv - Systems Biology 2021Quote: ... qRT-PCR reactions were performed using Universal PCR master mix (Applied Biosystems) and TaqMan gene expression assays (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... qPCR was performed using TaqMan™ Universal qPCR Master Mix (Applied Biosystems) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR was performed using TaqMan Universal PCR Master Mix (Applied Biosystems) in the presence of primers for SCN5A ...
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bioRxiv - Neuroscience 2022Quote: ... qPCR was performed using TaqMan Universal PCR Master Mix (Applied Biosystems, 4364338) on a Roche 480 II instrument ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and 10 µl of TaqMan Universal PCR Master Mix (4364340; Life Technologies). The PCR reaction was performed using the default cycling conditions (50°C for 2 min ...
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bioRxiv - Cell Biology 2022Quote: ... Pierce™ universal nuclease for cell lysis was purchased from Thermo Fisher Scientific Corporation (Waltham ...
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bioRxiv - Cell Biology 2022Quote: ... Universal TaqMan PCR master mix (20X) with AmpErase UNG (Thermo Fisher Scientific) was used ...
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bioRxiv - Cell Biology 2022Quote: ... and 75 U/ml of Pierce universal nuclease (Thermo Fisher Scientific, 88700), and lysed by freezer milling (SPEX SamplePrep 6870 Freezer/Mill ...
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bioRxiv - Neuroscience 2021Quote: ... and run with the TaqMan Fast Universal PCR MasterMix 2x (Applied Biosystems). The following TaqMan probes were purchased from ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was perfomed using TaqMan Universal 2x Master Mix (Thermo Scientific) and human TIMP-1 (Hs01092512) ...
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bioRxiv - Neuroscience 2020Quote: ... All reactions were performed using TaqMan Fast Universal PCR Mix (Applied Biosystems) and TaqMan Assays (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... and TaqMan® Fast Universal PCR Mix (Applied BioSystems, Foster City, CA) was used in PCR reactions ...
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bioRxiv - Plant Biology 2019Quote: ... and 5μl Taqman Universal PCR Master Mix (Applied Biosystems, Foster city, CA). The sequences for the primer-probe sets are provided in Table S2 ...
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bioRxiv - Microbiology 2019Quote: ... PCR was performed using the Taqman Universal PCR Master Mix (Applied Biosystems), according to the manufacturer’s instructions with the recommended cycling conditions ...
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bioRxiv - Immunology 2019Quote: ... Reactions were performed using a TaqMan Universal Master Mix II (Applied Biosystems) and TaqMan probe (hsa-miR-24-3p ...
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bioRxiv - Genomics 2021Quote: ... 30μl assays dispensed in TaqMan Universal PCR 2X master mix (Applied Biosystems) were performed on LightCycler 480 (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... and qPCR analysis performed using Taqman Universal Master Mix (Life Technologies, #4440038) using specific primers (Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed using TaqMan™ Universal PCR Master Mix (Life Technologies) and TaqMan probes against ...