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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... supplemented with 5% (v/v) fetal bovine serum (FBS) and 1% (v/v) antibiotic-antimycotic (Gibco). HEK293S GnTI- cells (ATCC CRL-3022 ...
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bioRxiv - Cancer Biology 2023Quote: ... and suspended in ice cold 50 µl A-Ri-Buffer (5% FBS, 1% GlutaMAX-I, Invitrogen, 35050-079 ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary AlexaFluor-conjugated antibodies (Life Technologies; 1:500 in 5% FBS, 0.1% Triton-X in PBS) with Hoechst 33342 dye were incubated for 1 h at room temperature in the dark ...
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bioRxiv - Molecular Biology 2024Quote: Confluent HFF cells were infected with ME49ΔKu80 parasites (1 parasite: 5 hosts) in DMEM media (Invitrogen) with 10% (v/v ...
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bioRxiv - Genomics 2023Quote: ... cells were washed 3 times with PBS for 5 min and stained with donkey anti-goat Alexa Fluor® conjugated secondary antibody (Thermo Fisher Scientific Inc., Cat. No. A110-55) at 1:500 for 1 hour at room temperature in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Cell Biology 2020Quote: ... Ovaries were washed with PBST for 15 minutes before staining nuclei with TO-PRO-3 (Life technologies, 1:1000) in PBST for 15 minutes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PBS was then washed 3 times and 1 times CellMask™ orange Actin Tracking Stain (Thermofisher; Catalogue number: A57244) and allowed to incubate for another 15 mins at RTP to stain for F-actin ...
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bioRxiv - Synthetic Biology 2022Quote: ... and used in a ligation reaction with the inserts (3:1 ratio) using T4 DNA ligase (Thermo Fisher, EL0011), at 22°C for 4–6 h ...
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bioRxiv - Neuroscience 2021Quote: ... tissue was incubated in 3% NDS-PB-TX primary antisera to FR (1:1000, Invitrogen #A6397, made in rabbit), FS (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... counted and total RNA was recovered from 3×106 cells using 1 ml of Trizol (Invitrogen, Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... Adult human dermal fibroblasts were purchased by ATCC and maintained in Fibroblast medium (3:1 DMEM: Ham’s F12 Nutrient Mixture, Gibco) supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2019Quote: ... washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500, Alexa Fluor 647, Invitrogen) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... ATS-bound peptides were washed with 50 mM ammonium bicarbonate (3×) and eluted with 1 mM TCEP (Thermo Scientific) for 30 min at 37°C with constant rotation ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The soluble fraction was then loaded onto 1 mL or 3 mL HisPur Ni-NTA Spin Columns (Thermo Scientific) and His-tagged protein was purified according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... Ligation reactions were performed by using a 3:1 insert/vector ratio with T4 DNA Ligase (Thermo-Fisher Scientific) and transformed into chemically competent Escherichia coli DH5α cells ...
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bioRxiv - Neuroscience 2020Quote: Glial primary cultures were established from cerebral cortices of C57BL/6J or Sfrp1-/- 1-3 days-old pups dissected in Ca2+/Mg2+-free Hank’s Balanced Salt Solution (HBSS, Invitrogen) and processed following standard procedures [79] ...
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bioRxiv - Immunology 2021Quote: ... kidneys from infected mice were harvested at 3 days post infection andplaced for 1 hour in RNAlater solution (Invitrogen). Kidneys were homogenized in Lysing Matrix C tubes (MPbio) ...
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bioRxiv - Cell Biology 2021Quote: ... embryos at the 3-6 somite stage were embedded oriented laterally in 1% low-melt agarose (Invitrogen, Carlsbad, CA) and imaged under bright field (to determine yolk elongation by taking major and minor axis measurements ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed 3 times with TBST and incubated with anti-mouse IgG secondary antibody (A16017, Thermofisher, 1:10,000) or anti-rabbit IgG secondary antibody (A16035 ...
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bioRxiv - Neuroscience 2020Quote: ... The next day slices were washed 3× in 0.3% PBS-T then incubated in Goat ∝ Chicken:488 secondary antibody (1:1,000, Invitrogen, A32931) for 2 h at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... Pellets were then resuspended in 500µl of solution containing a ratio of 3:1 of water to LDS Sample Buffer (4x) (Invitrogen) with a final concentration of 2mM DTT ...
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bioRxiv - Microbiology 2022Quote: ... incubated for 10 min in the dark at room temperature with FM 4-64FX (3 μg ml-1) (ThermoFisher). Cells were applied to a 5% agarose pad containing M9 minimal media with glucose (0.4%) ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 × 103 Panc-1 cells per 30 µl were pre-labeled with Hoechst 33342 (Thermo Fisher Scientific; Waltham, MA) for 1 hour prior to trypsinization and resuspended in tumor sphere-forming media (Irvine Scientific ...
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bioRxiv - Biophysics 2022Quote: ... and 1% v/v Triton X-100 along with 3 pellets of EDTA-free Pierce Protease Inhibitor (ThermoFisher Scientific), and 0.25 mg/ml hen egg white lysozyme ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Molecular Biology 2020Quote: The rat insulinoma cells (INS-1, 832/3)54 were cultured in RPMI-1640 medium (Thermo Fisher Scientific, 11875119) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... 500 μl of washing buffer was added (Tris-HCL 10 mM, NaCl 10 mM, MgCl2 3 mM, Tween-20 0.1%, BSA 1%, Invitrogen™ RNAseout™ recombinant ribonuclease inhibitor 0.04 U/μL ...
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bioRxiv - Microbiology 2020Quote: ... one for virus quantitation (TCID50) and the other for vRNA extraction in TRIzol (ThermoFisher, 15596026; 1 in 3 dilution). Samples were stored at -80°C until required ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines except BxPC-3 and SK-BR-3 were maintained in high glucose (4.5 g L-1) Dulbecco’s Eagle Medium (DMEM, ThermoFisher Scientific). BxPC-3 cells were maintained in RPMI medium (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... and passaged very 3-4 days with a 1:4-6 passage ratio using Versene solution (Life Technologies 15040066).
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bioRxiv - Genetics 2022Quote: Immunoprecipitations were performed using 1 μg of Anti-Trimethyl-Histone-3-Lys-4 (-Thermo Fisher Scientific; catalog# PA5-17420) overnight at 4 ◦ C ...
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bioRxiv - Physiology 2022Quote: ... The nucleic acids present in the pituitary gland cells were visualised with TOTO-3 (1:2000, Thermo Fisher Scientific). Sections were incubated with TOTO-3 for 15 min on an agitated surface ...
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bioRxiv - Neuroscience 2019Quote: ... harbouring the full-length cDNA coding for the human muscle 6-phosphofructo-1-kinase muscle isoform (PFK1-M)3 (accession number, NM_000289.1) using Lipofectamine LTX-PLUS Reagent (Life Technologies) according with manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... at a density of 1.2-1.6 million cells/mL using 25 kDa linear polyethylenimine (PEI) at a 3:1 PEI:DNA ratio in OptiMEM reduced serum medium (Gibco), with a heavy chain ...
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bioRxiv - Immunology 2020Quote: ... 1×106 CAR T cells were co-cultured with γ-irradiated K562-CD19 or K562-CD19-PD-L1 at 1:3 effector:target (E:T) ratio for 7 days and counted with the countess II Automated Cell Counter (ThermoFisher, USA).
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bioRxiv - Cell Biology 2019Quote: ... mESCs cultured in Serum+Lif medium were replated evey 3 days at a split ratio from 1:30 following dissociation with Trypsin 0.05% EDTA (Gibco). mESCs containing floxed alleles of b-catenin were a kind gift of professor Rolf Kemler (SR18 and NLC1 cell lines ...
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bioRxiv - Pathology 2020Quote: ... the sample was immersed in a 1:1000 v/v solution of TO-PRO 3 iodide (T3605, Thermo Scientific) in PBST overnight with light shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were washed 3 times with PBS where the second wash contained DAPI staining (dilactate, Invitrogen at 1:10,000). Coverslips were mounted onto glass slides using Fluoromount-G mounting medium (0100-01 ...
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bioRxiv - Immunology 2021Quote: ... Heat-inactivated sera (1:100 dilution) were serial diluted in 3-fold steps in cell culture medium (DMEM (Gibco), supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 3 hours with 1 μg/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Physiology 2020Quote: ... and cDNA synthesis was performed with 1 μg of total RNA following the manufacturer’s instructions of the 3’ RACE kit (Invitrogen). Polymerase chain reaction (PCR ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids (3 µg) were introduced into HEK 293 cells (1×106) by electroporation with Neon electroporator (Thermo Fisher Scientific) according to the manufacturers protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 3:1-DMEM/F12-medium was supplemented with 10% (v/v) heat-inactivated FBS (Thermo Fisher Scientific, #10270-106) and 100 μM taurine (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... Final confirmation of unroofing was by application of 1 nM 1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate (DiI, Invitorgen, ThermoFisher, Waltham MA) in stabilization buffer ...
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bioRxiv - Microbiology 2020Quote: hESC differentiation into endoderm was performed in serum-free differentiation (SFD) medium of DMEM/F12 (3:1) (Life Technologies) supplemented with N2 (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... Activated/Cleaved Caspase-3 (1:1000; Cell Signalling Biology, CAT#: 9661S and Thermo Fisher Scientific, CAT#: 66470-2-IG), Nestin (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... alongside aforementioned activating proteins and CellEvent™ Caspase-3/7 Green Detection Reagent (1:5000, ThermoFisher Scientific, Cat#C10423). Cells were imaged using the IncuCyte ZOOM System (Essen Bioscience ...
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bioRxiv - Cell Biology 2022Quote: ... Ovaries were washed with PBST for 15 minutes before staining nuclei with TO-PRO-3 (Life technologies, 1:1000) in PBST for 15 minutes ...