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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... at a 1:3 (µg DNA to µg PEI) ratio while the culture was detached with Trypsin-EDTA (Gibco). The cell density was assessed with the automated cell counter Countess II FL (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: 100 to 150 3-dpf zebrafish larvae were bathed in Yo-Pro-1 (4 µM, 30 min) (Invitrogen, Y3603), then washed 3 times in blue water to label lateral line hair cells ...
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bioRxiv - Neuroscience 2023Quote: Synaptic vesicles (purified from TKO mice) were labeled with Dil dye (1,1’-dioctadecyl- 3,3,3’,3’-tetramethylindocarbocyanine, Invitrogen; 1 µM) for 1 h at 22°C and dialyzed with the buffer containing 150 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... washed 3 times with PBT1% (1% Triton X-100 in PBS) and permeabilised using a 0.25% trypsin solution (Gibco) at 25°C when embryos were older than 24 hpf ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then washed with 1 × PBS and blocked in 3% BSA with 0.1% Triton-X 100 (ThermoFisher Scientific) and 0.1% Tween (Sigma ...
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bioRxiv - Genomics 2024Quote: ... These selected colonies were grown in King’s B medium consisting of 20 g l-1 Bacto proteose peptone No.3 (Gibco), 1.5 g l-1 Potassium phosphate dibasic trihydrate (P5504 ...
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bioRxiv - Immunology 2024Quote: ... VH and VL plasmids were mixed at a 1:3 ratio and transfected into Expi293F cells (Thermo Fisher Scientific), which were cultured at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA transfection was accomplished by mixing 1 µg of DNA pre-complexed with 3 µg lipofectamine 2000 (ThermoFisher 51985091) in 200 µl Opti-MEM (ThermoFisher 11668019 ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The supplemented-Basal Epithelial Medium (s-BEM) consisted of 3:1 mix of Ham’s F12 (Gibco, Life Technologies, UK) and DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The supplemented-Basal Epithelial Medium (s-BEM) consisted of 3:1 mix of Ham’s F12 (Gibco, Life Technologies, UK) and DMEM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... ASCEs were maintained on mitomycin C-treated 3T3-J2 feeder cells (a generous gift from the late Dr Howard Green) in F medium (3:1 [v/v] Ham’s F12 nutrient mixture:DMEM, high glucose (both from Life Technologies) supplemented with 5% FBS ...
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bioRxiv - Microbiology 2022Quote: ... blocked with 3% BSA and incubated with 1:1000 dilution of mouse anti-P30 (SAG1) primary antibody (Invitrogen MA183499). Cells were then permeabilized and incubated with 1:1000 dilution of rabbit anti-Toxoplasma primary antibody (Invitrogen PA17252 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All steps were carried out in serum-free differentiation (SFD) medium consisting of IMDM/F12 (3:1) (Life Technologies), N2 and B27 supplements (Life Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... The following day membranes were washed 3 × 10 min in TBST and incubated with anti-rabbit (Invitrogen, 1:20,000) or anti-mouse (Cell Signaling ...
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bioRxiv - Genomics 2023Quote: ... Chromosomes were stained with anti-Elys antibodies [65] (1:400) diluted in PBT containing 0.3% Triton X-100 and 3% Normal Goat Serum (Invitrogen) and counterstained with Dapi ...
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bioRxiv - Neuroscience 2023Quote: ... The following day membranes were washed 3 × 10 min in TBST and incubated with anti-rabbit (Invitrogen, 1:20,000) or anti-mouse (Cell Signaling ...
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bioRxiv - Cell Biology 2023Quote: ... D3 cells were cultured in DMEM/F12 (3:1) medium supplemented with 10% fetal bovine serum (Thermo Fisher Scientific), 8 ng/mL Cholera Toxin (CELL technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μL of washing buffer was added (Tris-HCL 10mM, NaCl 10 mM, MgCl2 3 mM, Tween-20 0.1%, BSA 1%, Invitrogen™ RNAseout™ recombinant ribonuclease inhibitor 0,04 U/μL ...
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bioRxiv - Immunology 2023Quote: ... and packaging vector psPAX2 were mixed in a 4:3:1 ratio in OPTI-MEM (Thermo Fisher Scientific, 31985070) and PEI (Polysciences ...
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bioRxiv - Genomics 2023Quote: ... and extracted total RNA using BCP (1-bromo-3-chloropropane; Life Technologies, Thermo Fisher Scientific, Inc., Waltham, MA, USA) with isopropanol precipitation ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were passaged 3 times per week at 1:10 dilution using Dulbecco’s Phosphate Buffered Solution (DPBS; Gibco 14190144) and trypsin (Gibco 25200072) ...
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bioRxiv - Neuroscience 2023Quote: ... then rinsed 3 more times in PBS containing a 1:1000 dilution of Hoechst 33258 nuclear dye (Invitrogen H3569) prior to confocal fluorescence imaging using a Nikon NSPARC confocal microscope ...
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bioRxiv - Genetics 2023Quote: ... Around 3 x 105 cells were electroporated with 1 μg of each episomal vector using Neon Transfection System (Thermofisher). The emerging ESC-like colonies were manually picked and transferred into 96-well plates coated with rhLaminin-521 (Thermofisher Catalog#A29248 ...
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bioRxiv - Cancer Biology 2023Quote: hESC differentiation into endoderm was performed in serum-free differentiation (SFD) media of DMEM/F12 (3:1) (Life Technologies) supplemented with N2 (Life Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... and ligation was performed at a 3:1 insert:vector ratio using AnzaTM T4 DNA Ligase Master Mix (ThermoFisher Scientific) supplemented with 10mM ATP ...
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bioRxiv - Microbiology 2023Quote: ... and a TRPMPZQ expression plasmid (Sm.TRPMPZQ or Fh.TRPMPZQ) containing a Flp recombination target (FRT) site (pcDNA5/FRT) at a 3:1 ratio using Lipofectamine 2000 (Invitrogen). Two days following transfection ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; S911, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... cryo-embedded in a 1:3 mixture of 30% sucrose and Optimal Cutting Temperature Compound (Thermo Fisher Scientific, USA), and stored at −80°C for future cryosectioning ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then incubated for 48-72 hours in a PBS solution containing 0.3-0.5% TritonX-100 and 0.05% NaN3 and streptavidin conjugated to either AlexaFluor568 or AlexaFluor633 (1:500; Invitrogen, UK). Slices were then washed in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were then incubated in 5% normal donkey serum (NDS) for 1 hour before primary antibody incubation (mouse anti-GFP, 1:1500, Life Technologies; rabbit anti-TH, 1:2000, Millipore) overnight at room temperature with NDS and PBS-Tx ...
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bioRxiv - Biochemistry 2022Quote: ... and 5 µg/mL and 5 units/mL penicillin-streptomycin (Gibco). One T175 flask of HEK293 cells of 70% confluency per condition was transiently transfected using 90 µg of EGFP-PABPi or EGFP-PABPi mut ...
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bioRxiv - Neuroscience 2021Quote: ... 5 U/ml penicillin and 5 U/ml streptomycin (Invitrogen, USA). Cells were plated on poly-D-lysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... using forward primer 5’-CACAGGAAGCCCTGGAAGCT and reverse primer 5’-GAGCAGGTCAGGTTTTTGGA (Invitrogen). SIGLEC22P was amplified similarly with forward primer 5’-GCACCTCAGAGTGGAAGGAC and reverse primer 5’-GAAGGGGTGACTGAGGTACA ...
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bioRxiv - Cell Biology 2021Quote: ... with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen) to generate the pcDNA3.1-KIR-CD33-V5/HIS vector ...
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bioRxiv - Biochemistry 2024Quote: hSSB2 was labeled with 5-IAF (5-iodoacetamido-fluorescein, Thermo Fisher) on the intrinsic cysteines ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 µL of 5% ammonium persulfate (APS) (Thermo Scientific, 17874). 50 µl was added to a strip of parafilm in a humidity chamber on ice ...
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bioRxiv - Biophysics 2023Quote: ... samples were incubated with a 5 μM DRAQ-5 (Thermo Scientific) DNA staining buffer at 37° C for 30 minutes with agitation ...
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bioRxiv - Biochemistry 2024Quote: ... 0.3 × 5 mm trapping column (5 µm, 100 Å, Thermo Scientific) and analyzed by nanoLC-ESI-MS/MS analysis using a Thermo Ultimate 3000 RSLC nanoUPLC coupled online to an Orbitrap Exploris™ 240 mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... containing 5 unit/mL penicillin and 5 ug/mL streptomycin (Gibco).
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bioRxiv - Developmental Biology 2019Quote: Prab-3::GFP was generated by cutting out Prab-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Developmental Biology 2019Quote: Pelt-3::GFP was generated by cutting out Pelt-3 from pCR®2.1-TOPO® (Invitrogen) (see above ...
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2019Quote: ... or caspases 3/7 (Caspase-3/7 Green ReadyProbes™ reagent with a DEVD sequence, Molecular Probes).
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bioRxiv - Cell Biology 2021Quote: ... 1–2 mg of VLDL were combined with 100 μL of 3 mg/mL 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate (DiD; Invitrogen) in DMSO and then re-isolated by ultracentrifugation ...
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bioRxiv - Biophysics 2022Quote: ... 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate (DiI) and (ii) 1,1’-Dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DiR) were both from Invitrogen. The standard lipids (i ...
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bioRxiv - Immunology 2023Quote: ... The caspase-3/7 activity assay contained CellEvent Caspase- 3/7 Green Detection Reagent (Thermo Fisher, C10723) at a final ...
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bioRxiv - Biochemistry 2023Quote: ... tRNAGln was 3’ biotin labeled with the Pierce ™ RNA 3’ end biotin labeling kit (Thermo Fisher) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... and washed 3 times with fresh ECGM and 3 times with ECGM/Hoechst 33342 (NucBlue™ ThermoFisher). After 10min incubation ...
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bioRxiv - Biochemistry 2023Quote: ... 3 mM DTT) and homogenized using a Dounce homogenizer followed by DNaseІ treatment (3 U/µl, ThermoFisher) for one hour on ice ...