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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/mL TPO and 40 ng/mL IL-3 from day 1 to day 6 with a media refresh every 2 days (all cytokines from Thermofisher). On day 8 ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA plasmids were cotransfected with the packaging and envelope plasmids using Lipofectamine 3000 at a ratio of 4:3:1 (#L3000001, Thermofisher) into LentiX cells ...
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bioRxiv - Genomics 2024Quote: ... and nuclei were washed once with nuclei suspension buffer (NSB; 10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 1% Superase RNA Inhibitor (Thermo Fisher), 1% 0.2mg/mL Ultrapure BSA (New England Biosciences)) ...
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bioRxiv - Immunology 2024Quote: ... the cells were washed 3 times with 1X PBS and treated with 1 μM C11-Bodipy 581/591 dye (Invitrogen) suspended in 1X PBS for 30 min in the dark at 37oC ...
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bioRxiv - Cell Biology 2024Quote: TRIM28 and TRIM24 knockdowns were performed similarly with the following modifications: 3×106 TRExRTA BCBL-1 cells in 100 μL of resuspension buffer T (Invitrogen) were nucleofected once with 2 uL of 100 μM of either Dharmacon human TRIM28 Accell SmartPool siRNA or OnTarget Plus human TRIM24 SmartPool ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies were detected using Alexa Fluor 488 labeled goat anti-rabbit IgG antibodies diluted 1:1000 in PBS containing 3% BSA (Invitrogen). Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was eluted in 100 µL of nuclease free water and precipitated at -80 °C for ≥ 3 hours with 1 µL of 15 mg/mL GlycoBlue coprecipitant (ThermoFisher), 10 µL of 3 M sodium acetate pH 5.5 ...
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bioRxiv - Immunology 2022Quote: ... The dissociated cells were washed 3 times with chilled PBS containing 1% heat-inactivated FBS and 2 mM EDTA (Gibco).
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bioRxiv - Microbiology 2022Quote: ... isolated primary cells were co-cultivated with gamma-irradiated mitotically inactivated NIH3T3 mouse embryonic fibroblasts (MEFs) in a 3:1 mixture of Ham’s F-12 nutrient mix (Life technologies) and DMEM supplemented with 5 % FCS ...
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bioRxiv - Cell Biology 2023Quote: ... The protein samples were denatured by adding 3× SDS sample buffer (containing 188 mM Tris-HCl, pH 6.8; 3% SDS; 30% glycerol; 0.01% bromophenol blue; and 1× Bond-Breaker TCEP solution from Thermo Fisher Scientific) and heating at 95°C for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Fms-like tyrosine kinase 3 ligand (Flt3- ligand; 100 ng/mL) and 1% penicillin-streptomycin (GIBCO, Grand Island, NY, USA), in the presence or absence of G-CSF at a dose of 100 ng/mL ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twelve hours after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated for 1 hr at room temp in the following secondary antibodies at 1:500 dilution in PBS + 3% BSA: Alexa Fluor 488 Goat Anti-mouse IgG2a (Invitrogen) (binds the CD24 primary antibody) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The next day cells were transfected with a mixture of 4 ug plasmid DNA and a 1:3 ratio of PEI (1mg/mL) in Opti-Mem (Gibco). Forty-eight hours later ...
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bioRxiv - Microbiology 2023Quote: ... mouse embryonic fibroblasts 3T3-J2 cells (kind donation from Howard Green, Harvard Medical School, Boston52) with keratinocyte culture medium cFAD (3:1 DMEM (Gibco) to Ham F-12 Nut Mix (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... and 100 µl of the homogenate were added to 1:3 v/v of TRIzol™ LS (Thermo Fisher Scientific) and incubated for 10 min at room temperature to inactivate the virus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twenty-four hours post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HA-Gα-LgBiT constructs and SmB-cmyc-PDZ-RhoGEF were co-transfected using a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). After 24 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Biochemistry 2023Quote: ... at 70 °C for 7 min then precipitated in 75% ethanol with 10% volume of sodium acetate (3 M, pH 5.5) and GlycoBlue (1 μL, Invitrogen AM9515). The RNA fragments were dissolved in nuclease-free H2O at a concentration of 100 ng/μL ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... and chromosome III probes (3 BACS covering approximately 1% of the chromosome) were labeled with AlexaFluor 488 (Molecular Probes, F32947).
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bioRxiv - Immunology 2023Quote: ... 1×106 primary human keratinocytes were seeded into the inserts and kept submerged in a 3:1 DMEM (Hyclone, #SH30243.01) and F12 (Gibco, #31765035) mixture with 10% FBS (Hyclone ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with primary antibodies (Supplemental Table 3) followed by secondary antibodies (goat anti-rabbit IgG Alexa 488, 1:200, Invitrogen; goat anti-mouse IgG Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with the indicated plasmids routinely using 1 μg of DNA mixed with 3 μl of Lipofectamine 2000 (Invitrogen) in Optimem (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Dephosphorylated mRNA fragments were precipitated in 100% ethanol with 40 μl 3 M sodium acetate and 1 μl linear acrylamide (AM9520, Ambion) at -20°C for >16 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with N-(3-triethylammonium propyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM 1-43FX, Invitrogen, Cat.-No. F35355) at 5.6 µg ml-1 ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were then washed three times for 3 min in PBST and incubated in an anti-rabbit AF488 (1:2000, Invitrogen) and DAPI (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... 1µg of DNA and 3µl of FuGENE® (1:3 ratio) were combined with 100µl of Opti-MEM™ (Gibco), vortexed and incubated at room temperature for 30 minutes before adding to the cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Transfection took place immediately after seeding with a 1:3 mass to volume ratio of DNA to Lipofectamine 2000 (Invitrogen). Twelve hours after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... with 60 μL of mobile phase A (0.1% FA in HPLC grade water) at 3 μL/min separated using a 50 cm EASY-Spray column (ES803, ThermoFisher) ramping mobile phase B (0.1% FA in HPLC grade acetonitrile ...
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bioRxiv - Biochemistry 2023Quote: ... and SARS-CoV-1 HexaPro S) at 3 µg/mL were placed into 384-well Nunc Maxisorp plates (ThermoFisher, 464718) in 1x TBS and incubated for 1 hour at 37°C followed by slap drying and blocking with 80 µL of Casein for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... separation was performed using a NuPAGE Novex 3%–15% Tris-Acetate Mini Gel with 1× NuPAGE Tris-Acetate SDS running buffer (Invitrogen). Following an overnight transfer of proteins onto nitrocellulose membranes ...
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bioRxiv - Developmental Biology 2023Quote: ... Dissolved tissue was subsequently washed 3 times with 1% FBS solution in 1x DPBS (Dulbecco’s phosphate-buffered saline, ThermoFisher Scientific) and passed through 75 μm and 35 μm cell strainers to remove debris.
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bioRxiv - Evolutionary Biology 2023Quote: ... and incubated with transfection mix containing 1 µg total of the indicated plasmid DNA and 3 µL Lipofectamine 3000 Transfection Reagent (Invitrogen) in Opti-MEM for 4-8 hours at 37 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Cell Biology 2023Quote: ... with 60 μL of mobile phase A (0.1% FA in HPLC grade water) at 3 μL/min separated using a 50 cm EASY-Spray column (ES803, ThermoFisher) ramping mobile phase B (0.1% FA in HPLC grade acetonitrile ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3×2 minutes with DPBS before a permeabilization solution (0.1% Triton-X (Fisher Scientific, 9002-93-1) in DPBS ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were also probed with a mouse monoclonal antibody targeting glyceraldehyde-3-phosphate dehydrogenase (mAb-α-GAPDH; 1:8000; ThermoFisher).
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bioRxiv - Molecular Biology 2024Quote: ... followed by a second incubation for 3 min in PBS with LysoTracker Red (L7528, Invitrogen; 1:2,000 in 1x PBS) and HOECHST (H1399 ...
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bioRxiv - Microbiology 2024Quote: OG1RF cultures were grown overnight in 3 mL of CBM at 37 °C and diluted 1:50 into an optically clear 96-well plate (Nunc MicroWell tissue culture- treated optical bottom plates) ...
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bioRxiv - Cell Biology 2024Quote: ... membrane for 3 h at 60 V and detected with anti-GFP primary antibody (1:2000 mouse monoclonal GF28R, Invitrogen), horseradish peroxidase-conjugated rabbit anti-mouse secondary antibody (1:10,000 ...
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bioRxiv - Genetics 2024Quote: ... Cells were seeded with 300 000 cells/well in 6-well plates and transfected 24 hours later with 1 µg of each minigene construct mixed with 3 µL of Lipofectamine 2000 Reagent (Invitrogen) per well ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3× 5min with 1×PBST and incubated with mouse or rabbit secondary antibodies conjugated with horseradish peroxidase (Invitrogen) for 1 h at room temperature followed 3 × 5 min wash by 1× PBST ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split every 2-3 days through 9 minutes incubation with EDTA 50mM pH=7.4 and 3 minutes of 0.25% trypsin (Gibco).
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bioRxiv - Neuroscience 2021Quote: ... was performed using the Applied Biosystems QuantStudio 3 Real-Time PCR system and analyzed with the QuantStudio 3 Design and Analysis software (v1.5.1,ThermoFisher). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: 3′-desthiobiotin labeling was carried following the manufacturer’s’ guidelines of Pierce™ RNA 3′ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Biophysics 2019Quote: ... Procaspase-3 (0.123 mg/mL protein extract) and caspase-3 (1.0 U) were assayed in 96-well microplates (Nunc™ MicroWell™ 96-Well ...
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bioRxiv - Microbiology 2022Quote: ... 3 µL RT reaction mix consisting of 3 µL of 50 µM random hexamers (Thermo Fisher, cat#48190011), 0.8 µL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...