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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 5 Isobutylcyclohexane 1 3 dione 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... After the secondary antibody another four 15min washes in PBST (here 0.5% tween) with 1% BSA were performed followed by a short 5min incubation in TO-PRO™-3 Iodide (642/661)(Invitrogen, cat# T3605, 1:1000 dilution). The animals were mounted in moviol with DAPCO on glass slides and stored at 4°C till imaging.
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed 5 x 5 mins and 1 x 1hr before corresponding secondary antibodies along with the nuclear stain Hoechst33342 (1:5000; Molecular Probes; H-3570) were incubated overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were placed in a thermocycler and when the temperature reached 55°C, 10 µL of enzyme mix (1 µL 10X ThermoPol Reaction buffer, 1 µL (5 units) Taq DNA polymerase (Thermo Fisher Scientific, 18038240) and 0.01 µL (0.025 units ...
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bioRxiv - Genetics 2019Quote: ... Membranes were incubated for 1 hour at room temperature in 1xTBST + 5% non-fat milk with primary antibodies (1:2000 α-Myc 9E10, 1:5000; α-Vma2 (Life Technologies); 1:5000 α-Sir2 (Santa Cruz Biotechnology) ...
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bioRxiv - Cell Biology 2020Quote: ... MCF10A were cultured in [5 mM] glucose DMEM:F12 (1:1 mixture of F:12 [10mM] glucose and [0 mM] glucose DMEM) (Life Technologies, 11765054 and 11966025) supplemented with 5% Horse Serum (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed three times for 5 min at RT in TNT buffer and incubated with 0.5 µg ml−1 DAPI and Alexa555-Streptavidin (Thermo Fisher, #32355, 1:1,000) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... Germany) were grown at 37°C and 5% CO2 in a 1:1 mixture of Opti-MEM + Glutamax (Thermo Fisher Scientific; 51985-026) and DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed by PBS with 0.1% Tween 20 (PBST) (three times for 5 min each) and incubated with Alexa Fluor secondary antibodies 1:500 (Thermo Fisher, #A-11036) for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... centrifuged for 5’ on 1.4 rpm and were incubated with Fc Blocker (1:100, in 1XPBS + 1%FBS, Thermo Fisher Scientific, Ghent, Belgium) for 30’ on ice ...
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bioRxiv - Biophysics 2022Quote: The GyrA14-E487C and the CcdA50-72 peptide (Table S1) were labeled with Fluorescein-5-Maleimide and 5-TAMRA (Tetramethylrhodamine-5-Maleimide) from ThermoFisher Scientific as per the manufacturer’s protocol as described previously (Aghera et al. ...
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bioRxiv - Neuroscience 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU; 5 mg per kg body weight, Invitrogen) dissolved in sterile phosphate buffer solution (PBS ...
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bioRxiv - Biophysics 2021Quote: ... 5 μL of PAA premixes with 5% streptavidin–acrylamide (ThermoFisher, Cat#S21379) of the defined rigidity 48 were promptly sandwiched between the activated dish and the micropatterned coverglass immediately after adding a curing catalyst (aminopropyltriethoxysilane (APS)) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM MgCl2 and 5 or 10 mM caged ATP (#A1048 Invitrogen) prior to membrane wrapping ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with 5 μg FK506 (Invitrogen Cat. #INH-FK5-5). Thymi were taken for flow cytometry 24 or 48h after FK506 was administered ...
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bioRxiv - Biophysics 2024Quote: ... The PepMap100 C18 (5 μm 0.3 x 5 mm, Thermo Fisher Scientific) and the ACQUITY BEH C18 (1.7 µm 1.0 × 100 mm ...
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bioRxiv - Molecular Biology 2024Quote: ... or 5 μl/IP anti-GFP (A-11122, ThermoFisher, 5 ul/IP), for 3 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... was used at 1:4000 in 5% milk and exposed with SuperSignal West Dura substrate (Thermo Fisher).
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bioRxiv - Cell Biology 2020Quote: ... cells were fixed for 5 min at room temperature with 1 % methanol-free formaldehyde (Thermo Scientific, #28906). Cells were lysed using ice-cold Farnham lab buffer supplemented with complete protease inhibitor cocktail (#4693159001 ...
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bioRxiv - Cell Biology 2020Quote: ... for 30 minutes at 37°C and 5% CO2 and counterstained with 1:10,000 Hoechst (Thermo Fisher). Parameters were set as follows ...
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bioRxiv - Microbiology 2020Quote: ... 200μl of sample was extracted with a 5:1 volume ratio of TRIzol LS (Ambion, Carlsbad, CA), utilizing standard manufacturers protocols and resuspending in 11μl water ...
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bioRxiv - Cell Biology 2019Quote: ... 1 µL of chromatide ChromaTide™ Alexa Fluor™ 488-5-UTP or 546-14-UTP (Thermofisher) were added to transcription reactions to fluorescently trace label mRNAs ...
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bioRxiv - Bioengineering 2021Quote: ... 5×105 THP-1 cells/ml were treated with 25 ng/ml phorbol myristate acetate (PMA, ThermoFisher) in RPMI 1640 with 10% FBS ...
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bioRxiv - Biochemistry 2021Quote: ... PAs were loaded with X-Rhod-1 AM (5 μM, Thermo Fisher Scientific Inc., Waltham, MA, USA) for 30 minutes at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions mixes were first incubated on ice for 5 min before adding 1 µl BMOE (Thermo Fisher) to a final concentration of 0.64 mM ...
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bioRxiv - Plant Biology 2020Quote: ... 1 mM EDTA and 5 mM DTT) with the addition of 1μl of Protease Inhibitor (Thermo Fisher) and 1 μl of RNAse inhibitor (RiboLock) ...
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bioRxiv - Microbiology 2021Quote: ... 5 ml of the prepared cultures were mixed 1:50 with ionic strength adjuster (ISA, Thermo Scientific) and measurements were started immediately monitoring the electric potential in mV every 5 s for a total of 60 min at room temperature under constant stirring ...
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bioRxiv - Molecular Biology 2021Quote: 0.63 mmole (1 equivalent) of the nucleoside-5’-monophosphate free acid (Santa Cruz Biotechnology or ACROS Organics) and 5 equivalents of 2-aminoimidazole hydrochloride (Combi-Blocks ...
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bioRxiv - Cancer Biology 2021Quote: ... Biological duplicates (5 x 106 cells per replicate) were homogenized in 1 ml of TRIzol Reagent (Invitrogen) and purified using the mirVANA miRNA isolation kit (Ambion ...
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bioRxiv - Cancer Biology 2020Quote: ... ANA-1 cells were incubated in serum-free media with CellTracker Red CMTPX Dye (5 μM, ThermoFisher) for at 37 °C for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... MRC-5 cells (ATCC) were cultured in EMEM supplemented with 10% FBS and 1% Penicillin/Streptomycin (Gibco).
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bioRxiv - Bioengineering 2022Quote: ... and blocked for 1 hr with a solution containing 0.5% TritonX-100 and 5% donkey serum (ThermoFisher). Primary antibodies used were rat CD68 (FA-11 ...
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bioRxiv - Biophysics 2021Quote: ... cell nuclei were stained by exposure for 5 min to 1 mg/100 ml Hoechst 33342 (ThermoFisher) in water.
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bioRxiv - Molecular Biology 2019Quote: ... in 5% fetal bovine serum (Atlanta biologics, cat # S11550) and 1% penicillin/streptomycin (Life technologies, cat# 15070) at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using 1 μL of 5 μM Powerup SYBR green PCR Master Mix (Applied Biosystems), 2 μL of dH2O and 2 μL of cDNA sample in a 10 μL reaction ...
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bioRxiv - Plant Biology 2019Quote: ... and 5 μL of Power SYBR® green RNA-to-CT™ 1-step kit (Applied Biosystems) that has limits of detection as low as 2 copies of the target gene ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor cells were stained with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate) Dye (1 μM, #C7025, Invitrogen) and seeded (5×104/well ...
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bioRxiv - Neuroscience 2019Quote: ... 5 μg of PSD-95 antibody was coupled to 1 mg of Dynabeads (Life Technologies, CA, USA) according to the antibody coupling kit protocol (#14311D) ...
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bioRxiv - Physiology 2021Quote: ... HRP-linked anti-rabbit IgG (1:10000 in 5% BSA for WB. NP40 (Thermal Fisher Scientific, 28324), Triton™ X-100 (T-9284) ...
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bioRxiv - Neuroscience 2019Quote: ... the fibroblasts were plated at 5×104 – 1×105 onto irradiated mouse embryonic fibroblasts (Gibco, #S1520‒100). On day 8 ...
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bioRxiv - Biophysics 2019Quote: Cells plated on dishes were labelled with 1:200 dilution of 5 mM Laurdan (D250, Molecular Probes), incubated at 37 °C for 30 min ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight 4°C incubation with rabbit polyclonal antibodies against claudin-5 (1:100; Life Technologies) and ZO-1 (1:200 ...
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bioRxiv - Synthetic Biology 2020Quote: 0.63 mmole (1 equivalent) of the nucleoside-5’-monophosphate free acid (Santa Cruz Biotechnology or ACROS Organics) and 5 equivalents of 2-aminoimidazole hydrochloride (Combi-Blocks ...
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bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
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bioRxiv - Immunology 2021Quote: ... Reactions were conducted with 5 μL RNA and TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems) with 500 nM primers and 200 nM probes ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed three times with 1×PBS for 5 min and stained with DAPI (ThermoFisher Scientific) for 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... anti-rabbit fluorescent secondary antibodies (Alexa Fluor 488; 1:1000) and Hoechst 33342 (5 μg/ml; Invitrogen) at room temperature for 1 hr ...
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bioRxiv - Genomics 2022Quote: ... Nuclear staining was performed with DAPI at 1:1000 in PBS for 5 minutes (ThermoFisher Scientific, #D1306), followed by 2 final washes in 0.1% Tween-20 in PBS and resuspension in PBS.
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bioRxiv - Microbiology 2022Quote: ... supplemented with 5% fetal calf serum (FCS) and antibiotics (0.1 unit penicillin, 0.1 mg.mL-1 streptomycin, GIBCO) at 37 °C in a humidified 5% CO2 incubator.