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Citations for Thermo Fisher :
2301 - 2350 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Differentiated iNeurons were then cryogenized as aliquots of 1 · 106 to 8 · 106 cells in heat inactivated fetal bovine serum (FBS, 10270-106, Thermo Fisher) containing 5% v/v dimethyl sulfoxide (DMSO) ...
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Simultaneous adjunctive treatment of malaria and its co-evolved genetic disorder sickle cell anaemiabioRxiv - Microbiology 2022Quote: ... Serial dilutions of Pf3D7 total RNA and DNA (ranging from 8 to 5000 pg/µl) with nuclease-free water (AM9935, Life Technologies) were used to generate a relative standard curve for pfr1 ...
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bioRxiv - Cell Biology 2022Quote: Dictyostelium discoideum cells were maintained in Hans’ enriched HL-5 media (1.4X HL-5 media with 8% FM, penicillin and streptomycin) at 22°C in petri dishes (Fisher Scientific; FB0875712). A full list of strains used in this study can be found in Appendix Table S3.
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bioRxiv - Microbiology 2023Quote: Experiments were conducted in solution with an initial composition of either 8 g/l (0.14 molal (m)) NaCl solution (ThermoFisher Scientific, 207790010), 5.8 g/l (0.14 m ...
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bioRxiv - Plant Biology 2024Quote: ... the genomic DNAs of DUF760-1 and 2 (without the stop codon) were cloned with specific primers (Supplemental Table 2) into the Gateway compatible vector pCR™8/GW/TOPO (Invitrogen) and then transferred to the vector pGWB540 with the YFP at the C-terminus using LR clonase (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were grown with DMEM/F12 containing 10% FBS on 8-well Lab-Tek chamber slides (Nunc Lab-Tek Chamber Slide System, Thermo Scientific) precoated with 0.1% gelatin in PBS overnight ...
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bioRxiv - Microbiology 2024Quote: ... 2.5 µL of the reaction were then denatured in Laemmli buffer and run on a 8-16% polyacrylamide gel by SDS-PAGE and stained with either Brilliant Blue R250 or Coomassie Fluor Orange (Molecular Probes) and visualized on a Bio-Rad ChemiDoc MP imager.
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bioRxiv - Microbiology 2024Quote: ... Purity of the recombinant PfPKG proteins was assessed by SDS-PAGE using NuPAGETM Novex® 3-8 % Tris-Acetate gels (Invitrogen) and expression was confirmed by Western blot analysis.
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bioRxiv - Molecular Biology 2024Quote: ... All Expi293F cells were cultured at 8% CO2 saturation and 37°C with shaking in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 4 mM L-glutamine.
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bioRxiv - Neuroscience 2024Quote: Astrocytes were FACS sorted as described above and plated in 8-well Lab-Tek II®CC2 Slide (Thermo Scientific, 1054941). Within 2-3 days after plating ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to 8% SDS-PAGE followed by Western blotting using iBlot 2 Dry Blotting System (Thermo Fisher Scientific). 1.0 μg/ml rabbit polyclonal anti-nucleolin antibody (ab22758 ...
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bioRxiv - Cell Biology 2024Quote: ... to form condensates (with 20% v/v PEG 8 kDa as needed) and overlaid with fluorescent dextrans of various sizes at 10 kDa (Invitrogen D1816), 40 kDa (Invitrogen D1842) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... of the homogenized protein-loading buffer sample were loaded in duplicates on a NuPAGE™ 3-8% Tris-Acetate gel (ThermoFisher) to be probed separately with FLAG and ATPase antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... Then the tubes were transferred into ice and added with 8 μL 5X First-Strand Buffer of SuperScript™ III RT kit (Invitrogen), 2 μL of 0.1 M DTT ...
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bioRxiv - Microbiology 2023Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher, catalog #A33952), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... TMT-labeled peptides were fractionated into 8 fractions using the high pH reverse-phase peptide fractionation kit according to manufacturer’s instructions (Thermo Fisher Scientific) (Pierce High pH Reversed-Phase Peptide Fractionation Kit ...
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bioRxiv - Genomics 2024Quote: ... Protein extracts were subjected to electrophoresis in 10% polyacrylamide SDS gels using Tris-glycine-SDS running buffer (Alaos) or in NuPAGE 3-8% Tris-acetate precast polyacrylamide gels (Thermo Fisher), with Tris-acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... All bacterial stocks were stored as aliquots at -80°C in brain heart infusion media (BHI; Fisher Scientific, DF0037-17-8) to minimize freeze-thaw cycles ...
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bioRxiv - Immunology 2024Quote: ... cleanup beads were used (0.8:1 beads/sample ratio) and then eluted in 10 µL of DNase/RNase-Free Distilled Water (10977- 049, ThermoFisher®). cDNA was quantified by the Quant-iT PicoGreen dsDNA Assay Kit (P7581 ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen rat ovarian follicles were homogenized in 400 µL lysis buffer (100 mM HEPES, pH 8, 4% SDS, 1 mM EDTA, and 1× Halt protease/phosphatase inhibitor cocktail (ThermoFisher Scientific) using Zirconia/glass beads in a bead beating grinder (FastPrep24 ...
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bioRxiv - Bioengineering 2024Quote: Knock-in percentage and cell counts were measured on day 10 of culturing (day 8 post-electroporation) by flow cytometry using the Attune NxT flow cytometer with a 96-well autosampler (ThermoFisher Scientific). Briefly ...
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bioRxiv - Cancer Biology 2024Quote: ... Human embryonic stem cell line H9 was cultured on the 6-well plate coated with the Vitronectin (5 μg/ml) and in the Essential 8 Flex medium (both Thermo Fisher). Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher ...
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bioRxiv - Biophysics 2023Quote: The cysteine-bearing Aβ40 variant was redissolved in 50 mM NaPi pH 7.5 and labelled with an 8-fold molar excess of Alexa Fluor™ 488 C5 Maleimide (AF488; Invitrogen Life Technologies) and incubated at room temperature for 2 h (Supplementary Figure 3) ...
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bioRxiv - Biophysics 2023Quote: ... and U-251 cells were seeded onto PLL-PEG-RGD-coated 8-well chambers in the respective medium with penicillin (1 unit/mL) and streptomycin (1 µg/mL; Gibco, Life Technologies) at densities between 0.5 x 104 to 2.5 x 104 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed once with DMEM/F-12 and then cultured in Glasgow’s MEM (WAKO) containing 8% Knockout Serum Replacement (KSR, Thermo Fisher Scientific), 1 mM sodium pyruvate (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... 14 min (100SPD) or 8 min (180SPD) total runtime with PepMap™ columns 150 μm x 15 cm (Thermo Fisher Scientific) in a trap&elute configuration ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... monolayers and Vero (African Green Monkey Kidney Cell) cell lines were cultured with 10% and 8% fetal bovine serum (FBS, Gibco, USA) DMEM (Dulbecco’s Modified Eagle’s Medium ...
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bioRxiv - Molecular Biology 2023Quote: ... 10μg of total protein were heat-denatured for 5 minutes at 100°C before loading onto NuPAGE 3– 8% TRIS Acetate Midi Gel (Novex, Life Technologies) and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Neuroscience 2023Quote: ... To revive hiPSCs from cryopreservation cells were thawed by incubation for 2 minutes at 37°C and retrieved by centrifugation (200 x g for 3 minutes) prior to resuspension in Essential 8 (E8, (A1517001, Life Technologies) medium with 1’RevitaCell ...
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bioRxiv - Immunology 2023Quote: ... Vesicles were subjected to high-speed centrifugation for 4 h at 55,000 g (25°C) and subsequently at 8 h at 75,000 g (4°C) with an ultracentrifuge (Sorvall RC M150GX, Thermo Fisher Scientific) using a fixed angle rotor (S150AT-0128 ...
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bioRxiv - Molecular Biology 2023Quote: Flp-In T-REx HeLa cells of the genotypes indicated in the figure legends were plated in 8-well Lab Tek II Chamber 1.5 German coverglass dishes (Thermo Fisher, 155409) in regular growth medium ...
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bioRxiv - Cell Biology 2023Quote: ... or grown into a monolayer in Nunc™Lab-Tek™ chambered #1.0 Borosilicate Coverglass System 8 chambers microscopy dish (ThermoFisher). cbBOECS were imaged in EGM-18 medium at 37°C and 5% CO2.
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bioRxiv - Pathology 2023Quote: ... The explants were cut (1.0 cm2) and cultured at an air-liquid interface on culture inserts (filter pore size 8 μm; #141082, Thermo Fisher Scientific) with a 1:1 mixture of Ham’s F-12 and DMEM supplemented with 0.3% FCS ...
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bioRxiv - Genomics 2023Quote: ... From this sample a cell clone was derived from primary cells isolated from bat kidney tissue and immortalized via the transfer and stable production of the Simian virus 40 Large T antigen (SV40LT). Kidneys were cut in small pieces (ca. 8 mm3) and incubated in 0.05% Trypsin-EDTA solution (Gibco # 25300-054) for 15 h at 4 ºC ...
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bioRxiv - Synthetic Biology 2023Quote: ... All primers and oligos were ordered from Integrated DNA Technologies (IDT) dried and resuspended in 10 mM Tris buffer (pH=8, Fisher Scientific).
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bioRxiv - Synthetic Biology 2023Quote: ... Soluble proteins were separated from cell debris by centrifugation in a fixed angle rotor (Thermo Fisher Scientific, Fiberlite F15-8×50cy) at 10,000 rpm for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... lysed in 8 M urea and quantified using the Pierce™ Rapid Gold BCA Protein Assay Kit (Thermo Fisher Scientific, USA). Samples were denatured in Laemmli buffer at 95 °C and run at 170 V on a 10% acrylamide gel with a 4% stacking gel ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were rinsed twice with PBS and lysed in 8 M urea 50 mM Tris pH 7.4 containing protease inhibitor (Thermo Fisher Scientific) and DTT ...
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bioRxiv - Cell Biology 2023Quote: ... the two solutions were rapidly mixed together with 15 pumps on the syringe handles and instantly deposited into a well in an 8-well Lab-Tek dish (Thermo Scientific). We sought to maintain similar collagen fiber architecture when IPN stiffness and viscoelasticity was tuned ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Cancer Biology 2023Quote: ... 200 cells were seed in each well of 8-well chambers coated with a thick layer of matrigel (Thermor Fisher Scientific) and covered with 200 ul full-medium containing 3% matrigel ...
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bioRxiv - Genetics 2023Quote: ... IP and input DNA were purified using ChIP elution buffer (250 mM NaCl, 1% SDS, 10 mM Tris-Cl pH 8, 1mM EDTA) and incubation with proteinase K (ThermoFisher Scientific) overnight at 65°C ...
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bioRxiv - Genomics 2023Quote: ... Immunoblotting was performed according to standard procedures with the following modifications: a 3-8% tris acetate gel (Thermo Fisher Scientific, EA03752) was used and samples were run for 2 hours 15min at 80V at 4 °C in 1X tris-acetate running buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no. 150239, Thermo Scientific) and then cultured at 22°C until sexual maturity ...
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bioRxiv - Genomics 2023Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Immunology 2023Quote: Human ileocecal adenocarcinoma cells (HCT-8) (ATCC) were grown on coverslips in DMEM supplemented with 10% cosmic calf serum (CCS) (Thermo Fisher). 200,000 purified oocysts were excysted and seeded on coverslips when HCT-8 cells were 80% confluent ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Bioengineering 2023Quote: ... The stable iPSCs expressing the CRISPR repressor were cultured in a xeno-free and feeder–cell-free state in Essential 8™ Flex Medium (Gibco) along with the addition of Essential 8™ Flex Supplement (50X ...
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bioRxiv - Cell Biology 2023Quote: ... a reducing and oxygen-scavenging buffer optimized for dSTORM blinking (Halpern, Howard, & Vaughan, 2015) comprising 100 mM Tris-HCl (pH 8, J22638-K2, Thermo Scientific), 10% (w/v ...