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Citations for Thermo Fisher :
2251 - 2300 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Cells were seeded on poly-L-lysine-coated (MW > 300,000, 0.1 mg/ml, Biochrom) 8-well chamber slides (Nunc Lab-Tek II) and transfected the next day (50-70% confluency) ...
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bioRxiv - Biochemistry 2021Quote: ... then lysed in Triton lysis buffer (1% Triton, 150 mM NaCl, 50 mM Tris pH 8, 1× Halt protease-inhibitor cocktail (Thermo Scientific)) ...
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bioRxiv - Cancer Biology 2021Quote: ... The fluorescent reaction was measured every 40 sec for 8 min and subsequent every 5 min for 6 times using a microplate reader (Thermo Scientific) with enzyme kinetics model ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Immunology 2020Quote: ... 35 and 42 dpv by venepuncture of the external jugular vein: 8 mL/pig in BD SST vacutainer tubes (Fisher Scientific) for serum collection and 40 mL/pig in BD heparin vacutainer tubes (Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... ΔA ΔfabR ΔserB was generated at the OSU Unnatural Protein Facility as previously described.8 DH10b was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were then analysed by SDS-PAGE on 8% polyacrylamide gels using a PageRuler™ Prestained Protein Ladder (Thermo Scientific, 26616). Proteins where then transferred onto a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells were transiently transfected with the indicated siRNA at a final concentration of 8 nM using Lipofectamine 2000 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... An estimated 60 µg of protein was loaded per well on a NuPAGE Novex 3-8 % Tris-Acetate Protein gel (Life Technologies), separated by electrophoresis and blotted on polyvinylidene fluoride membranes ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Neuroscience 2020Quote: ... heated at 95 °C for 5 min and were run in on 8–16% NuPAGE SDS-PAGE gradient gel (Life Technologies) in MOPS SDS Running Buffer (Life Technologies ...
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bioRxiv - Immunology 2020Quote: Sh3gl1-/- and WT littermates were immunized at 8-10 weeks of age by intraperitoneal injection of 50 μg NP-CGG in alum (Thermo Fisher). Blood samples were taken before immunization and at 7 and 14 days after immunization ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted RNA from sets of 8 adult males (2~4 day old) in triplicate from each RNAi cross using TRIzol (Catalog# 15596-026, Invitrogen, USA), treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs ...
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bioRxiv - Neuroscience 2021Quote: All iPSC lines were maintained on vitronectin-coated plates and fed with Essential 8 (E8) + E8 supplement media (ThermoFisher, CAT#: A1517001).
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bioRxiv - Microbiology 2019Quote: ... Crosslinking was performed by incubating purified pUL7:pUL51(8–142) with DSSO or DSBU and mass spectra were acquired on an Orbitrap Fusion Lumos (Thermo Fisher). pUL7:pUL51(8–142 ...
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bioRxiv - Immunology 2021Quote: ... 2.5 x105 CFSE-labeled CD8+ T cells were cultured with J774M cells at varying ratios (1:1 – 1:8 J774M:T cells) and anti-CD3/CD28 beads (ThermoFisher, cat. 11453D) at a bead-to-T cell ratio of 1:1 per the manufacturer’s instructions for T cell activation ...
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bioRxiv - Immunology 2020Quote: 7575 naïve CD4 cells were isolated from lymph nodes of CCT8T+/+ and CCT8T−/− mice at 4-8 weeks of age by FACS sorting directly into lysis buffer (RIPA buffer (ThermoFisher Scientific) + 4 % IGEPAL CA-360 (Merck) ...
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bioRxiv - Physiology 2020Quote: ... Samples were then subjected to offline reversed phase fractionation (8 fractions) using a Pierce High-pH Reversed Phase Fractionation Kit (Thermo Fisher) according to the manufacturer’s instructions for fractionation of TMT-labeled samples.
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bioRxiv - Immunology 2020Quote: ... 42 and 56 days post-immunisation (DPI) by venepuncture of the external jugular vein: 8 mL/pig in BD SST vacutainer tubes (Fisher Scientific) for serum collection and 40 mL/pig in BD heparin vacutainer tubes (Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Developmental Biology 2021Quote: ... FAPs were plated at a starting density of 3 X 104 cells/cm2 and grown for 8 days in DMEM (Life Technologies) containing 5% FBS plus 1% Pen/Strep ...
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bioRxiv - Developmental Biology 2020Quote: ... For the detection of Gli3 10μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Developmental Biology 2020Quote: PSCs grown in Essential 8 Flex medium (see Supplementary material for details on PSC generation, characterization and maintenance) were dissociated by TrypLE (Life Technologies) to single cells and seeded on human recombinant laminin 521 (BioLamina)-coated plates in Essential 8 Flex medium at a density of 7 x 104 cells/cm2 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were cut in 16 μm coronal sections into 8 series that were mounted directly on slides (Superfrost Plus; Fisher Scientific) and stored at −45°C until receptor autoradiography was performed ...
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bioRxiv - Genomics 2021Quote: ... Then 100 μ L of this solution was gently mixed with 8 μ L of Lipofectamine™ 2000 (lipo) (Invitrogen, USA). A solution of lipo and siRNA (total volume 200 μ L ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... interleukin-6 and -8 (IL-6 and IL-8) human Instant Elisa™ kits and matrix metalloproteinase-1 (MMP-1) human Elisa kit from ThermoFisher Scientific.
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bioRxiv - Cancer Biology 2020Quote: ... The second half of the data (runs 5-8) was obtained after peptide separation on 75 μm columns packed with 2.6 μm Accucore beads (Thermo Fisher Scientific). Peptides were injected onto 30-40 cm ...
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bioRxiv - Immunology 2022Quote: ... Cross-linking occured for 30 min in a rolling tube after which beads were washed with 0.2 M ethanol amine pH 8 (#149582500, Thermo Fisher Scientific) to quench the crosslinking reaction.
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bioRxiv - Neuroscience 2022Quote: Human induced pluripotent stem cells (hiPSCs) were maintained in feeder-free monolayers on Geltrex (ThermoFisherScientific) and fed daily with Essential 8 medium (Life Technologies). When confluent ...
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bioRxiv - Neuroscience 2022Quote: ... They were cultured in 37C with 5% CO2 on geltrex-coated tissue culture plates in Essential 8 complete medium (E8; Gibco, #A1517001) with daily media changes ...
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bioRxiv - Cell Biology 2022Quote: ... and 4.8 µL cDNA (diluted 1:20 prior to use) was added to each well of qPCR plates (REF 4346906, Life Technologies). Samples were run on a StepOnePlus™ RT-qPCR machine (Applied Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... cells were maintained at a density of 2-8×105 cells/ml in Roswell Park Memorial Institute (RPMI) 1640 Medium (GIBCO, USA) and supplemented with 10% horse serum (GIBCO ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing the virus was then used fresh or stored by snap-freezing aliquots supplemented with 8 μg/ml polybrene (Fisher Scientific) in ethanol/dry ice and stored at -80° C ...
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bioRxiv - Biophysics 2022Quote: ... SOFI raw data was obtained using a buffer containing: 200 µl buffer C complemented with 8 mM KI (CAT: 373651000 - Thermo Scientific) and 300 mM MgCl2 (Product nr ...
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bioRxiv - Developmental Biology 2022Quote: ... Fly ovaries from 2-to 8-day old females were dissected in Schneider’s Drosophila Medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (Seradigm FBS ...
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bioRxiv - Immunology 2022Quote: ... Cell debris was removed from lysate containing soluble protein by centrifugation at 35,000 x g and 4 °C for 45 min (Sorvall LYNX 6000 centrifuge, F21-8×50y rotor, Thermo Fisher Scientific). Proteins were further purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Evolutionary Biology 2022Quote: ... After adding 8 μL of the master mix to wells of a 384-well MicroAmp Optical Reaction Plate (Applied Biosystems 4309849), 2 μL of genomic DNA sample which had been diluted 2:1 with nuclease free water was added ...
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bioRxiv - Plant Biology 2022Quote: ... T-DNA insertion position was confirmed with gene-specific and T-DNA-specific primers (Supplemental Table 8) with Phire Plant Direct PCR Master Mix (Thermo Scientific) according to the manufacturer’s guidelines.
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bioRxiv - Microbiology 2022Quote: ... were loaded and separated on 8% and 12% SDS-PAGE gel and transferred onto a polyvinylidene difluoride transfer membrane (Thermo Fisher). Immunoblotting was performed by using the corresponding antibodies ...
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bioRxiv - Microbiology 2022Quote: Proteins (8 μM final) were incubated with 0.16 mM of (2'-(or-3')-O-(trinitrophenyl) adenosine triphosphate (TNP-ATP) (Thermo Fisher Scientific), in 50 μl of Binding Buffer (10 mM HEPES-KOH (pH 8) ...
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bioRxiv - Neuroscience 2022Quote: ... and diluted to a final concentration of 8 × 104 cells/μL in sterile 1x PBS (pH 7.4, without calcium or magnesium; Thermo Fisher Scientific). Cells were stored on ice until transplantation ...
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bioRxiv - Microbiology 2023Quote: Transfection studies were conducted in HCT-8 and HeLa cells by the standard transfection protocol employing reagents lipofectamine 2000 and lipofectamine LTX (Invitrogen, USA) respectively ...
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bioRxiv - Physiology 2023Quote: ... 8 mg of the soluble protein fractions were pre-cleared with 200 μL of protein G-magnetic Dynabeads (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... the midbrain or cortical tissue from the 6-8 embryos were pooled together before digestion with Trypsin-EDTA 0.12% (Life Technologies, USA) for 7 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 2-3 mg/mL proteins were applied to glow-discharged M026-Au300-R20/20 Copper grid (CryoMatrix) and blotted for 3 s at 8 ℃ with 100% humidity and plunge-frozen in VitroBot Mark IV (Thermo Fisher).
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bioRxiv - Molecular Biology 2022Quote: ... MF002 - plus-10) and the PCR products were fractionated on 8% TBE gel stained with SYBR Gold nucleic acid stain (Invitrogen, S11494). Quantitative PCR (qPCR ...
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bioRxiv - Plant Biology 2022Quote: ... DNA concentration was quantified using Qubit and the quality of DNA was visualized using 8% TBE gel (Invitrogen, Cat. No. EC62152BOX) performed at 230 V for 30 min ...