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Citations for Thermo Fisher :
2451 - 2500 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 10μg protein was loaded per well on 8% acrylamide gels with prestained standard molecular weight markers (PageRuler prestained protein ladder; Thermo Fisher Scientific) and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2021Quote: ... Overexpressed myc-flag-tagged cadherin-8 was immunoprecipitated using the Pierce c-My-tag IP/Co-IP kit according to the manufacturer’s protocol (Thermo Fisher Cat#23620). Co-immunoprecipitated proteins were determined by Western blot analysis.
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bioRxiv - Cancer Biology 2021Quote: ... A total amount of 30 µg protein was loaded per lane of NuPAGE Tris-Acetate Protein Gel 3-8% (Life Technologies, EA0375BOX) and separated according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... 20 μl of untreated and of ProK treated PMV (before dilution for FRET) was loaded to 8 % SDS-PAGE gels and the proteins were stained with GelCold Blue Stain (Thermo Fisher Scientific). A control in which PMSF was added for 15 min at room temperature before adding ProK also prepared and loaded on the gel.
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bioRxiv - Physiology 2021Quote: ... aliquots of 30µg of protein were prepared in native Tris glycine sample buffer and analysed from NuPAGE 3-8% Tris-Acetate polyacrylamide gel electrophoresis in a tris-glycine native running buffer (Thermo Fisher Scientific). After 2-hours of migration at 130 V ...
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bioRxiv - Neuroscience 2021Quote: ... Human iPS cells were maintained and propagated as colonies in feeder-free conditions in Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES qualified (Corning ...
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bioRxiv - Microbiology 2021Quote: ... and sacculi were spun down at 22,000 x g at 40 °C for 30 min in a Heraeus Multifuge X1R using a Fiberlite™ F15-8 x 50cy Fixed Angle Rotor from Thermo Scientific. SDS was removed by washing the sacculi with 60 °C pre-warmed Millipore water several times until no SDS was detected by the methylene blue assay [Hayashi ...
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bioRxiv - Neuroscience 2020Quote: ... The middle segment was used to cut 8 μm serial sections using rotary microtomes (HM 200 and HM 355; Thermo Scientific, Germany) which were mounted on glass slides ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 8% of fetal bovine serum (FBS) and 1% of penicillin-streptomycin (PES 10, 000 u/mL, Thermo Fisher, NY, USA). Macrophages were seeded over sterile coverslips in a ratio of 250 × 103 / cm2 and they were maintained at 5% CO2 atmosphere at 37 °.
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bioRxiv - Microbiology 2020Quote: ... U-2 OS cells were cultured to 80% confluency in Nunc™ 8-well Lab-Tek II Chambered Coverglass slides (Thermo Scientific). Upon infection ...
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bioRxiv - Microbiology 2020Quote: ... EBV producer HEK-293 cells stably transformed by BART-repaired B95-8 based EBV BAC system encoding GFP (45) were cultured in RPMI 1640 (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... maintenance culture was carried out for all cell lines including human ES cells using albumin-free Essential 8 Medium (Thermo Fisher Scientific) in six-well feeder-free culture dishes coated with 5 μg/mL vitronectin (VTN-N ...
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bioRxiv - Microbiology 2020Quote: ... Cover slips were then washed three times for 5 min in wash buffer and subsequently mounted on slides with 8 μl of prolong glass (Invitrogen, cat. # P36984) or mowiol ...
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bioRxiv - Cancer Biology 2020Quote: ... A431RON (Figure 3A-B and 4) or A431RON-K1114M (Figure S3B) cells were seeded in 8-well chamber slides (Nunc Lab-Tek) at a density of 30,000/well and allowed to adhere overnight ...
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bioRxiv - Cell Biology 2021Quote: ... An aliquot of lysate was taken and diluted 1:5 in 8 M Urea from which protein concentration was determined by BCA assay (Thermo Fisher Scientific). Aliquots corresponding to 100 μg of protein were subsequently diluted in SDC buffer into a 96-well deep-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... BV-2 immortalized microglial cells (Blasi et al. 1990) (passage 3 to 8) cultured in macrophage serum-free medium (ThermoFisher 12065-074) were used ...
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bioRxiv - Cancer Biology 2020Quote: ... After 24 h, CCK-8 (Dojindo, Japan) was used to measure drug sensitivity at 450 nm using a microplate reader (Thermo Fisher, USA) after incubating at 37 °C for 1–2 h.
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bioRxiv - Cancer Biology 2021Quote: ... colonoids were dissociated using TrypLE and replated in an 8-well glass-bottom chamber slide (Nunc™ Lab-Tek™, Cat#155411). Three to four days later ...
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bioRxiv - Cell Biology 2021Quote: ... On-feeder hiPSC cultures were gradually transitioned to feeder- free conditions by weaning off from the standard HuES media to Essential 8™ medium (Gibco, #A1517001) and maintained on hESC-qualified Matrigel coated surface ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10 mM ATP in kinase buffer (20 mM Tris pH 8, 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 1X Phosphatase inhibitor (Thermo Scientific, #1862495)) at room temperature for 2 h ...
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Biophysics 2022Quote: ... followed by an 8× His tag and HRV 3C protease cleavage site was cloned into the modified pFastBac1 (Invitrogen, Carlsbad, CA, USA) vector ...
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bioRxiv - Cell Biology 2022Quote: ... Human iPSC lines were cultured feeder-free on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix and maintained in Essential 8 Flex Basal Medium (Thermo Fisher Scientific) supplemented with Essential 8 Flex Supplement (50x ...
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bioRxiv - Molecular Biology 2022Quote: ... The retinas were washed in PBS for 30 min at least 8 times at room temperature and incubated with anti-IB4 (I21412, Invitrogen, 1:200) and Alexa Fluor 488 goat anti-rabbit IgG (A11008 ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were killed with 10 μL of 6X SDS loading buffer and pooled or directly loaded onto a 15-well Novex Wedgewell 8% Tris-Glycine Gel (Thermo Fisher Scientific) and ran at 175 V for ~ 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Cell Biology 2022Quote: ... 10 μL of sample (which is the equivalent of 10 embryos) was loaded into each lane of a 3-8% Tris-Acetate pre-cast SDS-PAGE gel (Invitrogen, Thermo Fisher Scientific) and afterwards transferred from the gel onto a nitrocellulose membrane (BIO-RAD ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: DRG cultures for immunocytochemistry and RNAscope experiments were prepared as described above with the neurons plated on 8-well Chamber Slides (154534; Nunc Lab-Tek). The neurons were resuspended in culture media ...
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bioRxiv - Neuroscience 2019Quote: Wild-type C57/BL6J mice of either gender (aged 8-10 weeks) were used as recipients of yellow-green microsphere (polystyrene FluoSpheres™, 505ex/515em nm, Invitrogen, USA) transplantation ...
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bioRxiv - Cell Biology 2021Quote: ... enzyme activity was stopped with 8 ml of resuspension media (DMEM/F12 with glutamax supplemented with 15%FBS and 1% pen/strep; Thermo Scientific, CA). The digestion mixture was passed through 100 μm cell strainer and centrifuged at 150 x g for 8 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Denatured samples from both the total lysates and surface fractions were separated on NuPAGE 3-8 % Tris-Acetate protein gels (Thermo Fisher Scientific) at 200 V for 40 min ...
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bioRxiv - Molecular Biology 2020Quote: The human embryonic stem cell (hESC) line Hues9 (H9) was obtained from the Wicell Research Institute (Madison, WI) and maintained in Essential 8 media (Thermo Fisher Scientific) on hESC-Qualified Matrigel (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... protein samples were boiled at 95°C for 5 min before separating on Novex WedgeWell 8–16% Tris-Glycine Mini Gels (Thermo Fisher Scientific). Protein samples were transferred to polyvinylidene fluoride membrane (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... all sections were autoclaved for antigen retrieval and immunohistochemical stained with anti-phosphorylated antibodies AT-8 (pSer202/pThr205, MN1020, Invitrogen, 1:250). Immunolabeling was then examined using a DM4000 microscope (Leica ...
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bioRxiv - Cell Biology 2021Quote: The tomograms showed in the Figure 8 and 9 were performed on Glacios equipped with a field emission gun and operated at 200 kV (Thermo Fisher Scientific) and a Falcon 3 direct electron detector ...
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bioRxiv - Microbiology 2021Quote: ... mutations were inserted in fragment 8 (Extended Data Table 3) using the GENEART site-directed mutagenesis system (Thermo Fisher Scientific, cat# A13312) according to the manufacturer’s protocol with the following primers ...
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bioRxiv - Developmental Biology 2021Quote: ... H9 cells were cultured at 37°C and 5% CO2 in feeder-free conditions in Essential 8™ (E8 medium (Gibco, Life Technologies, Carlsbad ...
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bioRxiv - Developmental Biology 2022Quote: ... for 5 minutes at RT and then split with 1:8 ratio into 6 well plates pre-coated with 1:200 diluted geltrex in DMEM/F12 medium (Thermo Scientific A1413201) for 30 minutes at 37C ...
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bioRxiv - Bioengineering 2022Quote: ... culture dishes were coated at 4 °C overnight with collagen I at a 1:8 concentration diluted in ddH2O (Gibco, #A10483-01). The next day ...
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bioRxiv - Microbiology 2022Quote: ... The samples were sonicated and heated at 72°C for 10 minutes before loading 20 μl/well on NuPAGE™ 3 to 8% Tris-Acetate gels (ThermoFisher, EA03785BOX) alongside high molecular weight markers (ThermoFisher LC5699) ...
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bioRxiv - Microbiology 2022Quote: Mammalian cells were seeded at a density of 8×105 in a 96-well tissue culture plate (Black Nunc; Thermo Fisher Scientific), respectively and allowed to attach overnight at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Cell Biology 2022Quote: ... templated and sequenced using either Ion 550™ Kit-Chef and Ion 550 Chip Kit as one 16-plex library pool or Ion 540™ Kit-Chef and Ion 540 Chip Kit as one 8-plex library pool (Thermo Fisher). Two independent sequencing analyses were performed on a panel of eight samples ...
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bioRxiv - Cell Biology 2019Quote: ... placed into a ANSI/SLAS microplate format compatible 96-tube racks (8 × 12) and covered with the supplied top (Thermo Fisher Scientific). The covered tubes in racks were transported to a Gammacell 40 137Cs irradiator (Atomic Energy of Canada Ltd. ...
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bioRxiv - Biophysics 2019Quote: iPSCs were cultivated for several passages under feeder-free conditions (in the Essential 8™ Medium on Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific). 3-4 days before differentiation ...
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bioRxiv - Biophysics 2019Quote: ... iPSCs were cultured in the Essential 8™ Medium on a Geltrex LDEV-Free hESC-Qualified Reduced Growth Factor Basement Membrane Matrix (ThermoFisher Scientific) after transferring from the feeder layer ...
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bioRxiv - Neuroscience 2019Quote: ... the pH was adjusted to 7.4 using 1 M Tris pH 8 and then dialyzed overnight at 4°C in Hartmann’s using a 10 kDa dialysis membrane (Fisher Scientific, Loughborough, UK). The concentration of IgG present after dialysis was determined using a modified Lowry assay (DC protein assay ...
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bioRxiv - Cell Biology 2020Quote: ... Equal masses of lysates were separated by SDS-PAGE using either 4-12% Bis Tris gels or 3-8% Tris acetate gels (Thermo Fisher Scientific). All IRS2 immunoblots were separated on 3-8% Tris acetate gels with the exception of those shown in Figures 5A and S4B ...
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bioRxiv - Biophysics 2019Quote: ... Cells were seeded 1 or 2 days prior the experiment at low density (10000-12000 cells per well) in a glass bottom 8-well Lab-Tek II chambered coverglass (Thermo Scientific Nunc, 155383).
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bioRxiv - Synthetic Biology 2019Quote: ... the AAV helper plasmid carrying the rep and cap genes of AAV serotype 2 and (iii) an adenoviral plasmid providing helper functions for AAV production using 8 μl TurboFect Transfection Reagent per well (Thermo Fisher Scientific). 72 hours post transfection ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells or EphA2 HEK293T stable cells were seeded (1 x 104 cells/well) into 8-well tissue culture chambered coverglass slides (Thermo Scientific; 12565338) and cultured for 36 hours ...