Labshake search
Citations for Thermo Fisher :
2201 - 2250 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2019Quote: ... Cells were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates for activated caspase-8 (10 µM Z-IETD-R110; Molecular Probes), caspase-9 (10 µM ZLEHD-R110 ...
-
bioRxiv - Microbiology 2020Quote: ... Human nasal fluid samples were sonicated on ice (5 seconds at amplitude of 8 um; Fisher Scientific Model 705 Sonic Dismembrator) to create homogenous samples ...
-
bioRxiv - Cell Biology 2020Quote: ... 100 μg of anti-rabbit IgG was incubated with 8 μg of Alexa Fluor 750 N-Succinimidyl Ester (Thermo Fisher Scientific) in 100 mM NaHCO3 (pH 8.3 ...
-
bioRxiv - Neuroscience 2021Quote: ... All data were normalized to the total protein content determined in protein pellets after neutralization with 10 N NaOH and sonication in 2% SDS plus 8 mM EDTA using a bicinchoninic acid assay (ThermoFisher Scientific) and bovine serum albumin as a standard.
-
bioRxiv - Microbiology 2021Quote: ... One microliter of G3 phages were denatured at 94°C for 8 min and used as a template for PCR using Phusion High-Fidelity PCR Master Mix (Thermo Fisher). The amplified DNA fragment was purified using QIAquick Gel Extraction Kit (Qiagen ...
-
bioRxiv - Microbiology 2021Quote: ... cells were washed once with PBS (pH 8) and incubated with 1 mg/ml of EZ-link Sulfo-NHS-biotin (Thermo Fisher) in PBS (pH 8 ...
-
bioRxiv - Cancer Biology 2021Quote: ... emerging clones were manually picked and cultured under feeder-free conditions using Geltrex-coated dishes and Essential 8 medium (Life Technologies). iPSC clones were maintained in culture for at least 15 passages to ensure stable pluripotency before characterization was conducted by the iPSC – cell reprogramming core facility of CHU Sainte-Justine ...
-
bioRxiv - Biochemistry 2020Quote: ... 1*10^8 yeast were pelleted and resuspended in 500 µl PBM containing 100 nM Streptavidin-Alexa Fluor 647 conjugate (Thermo Fisher) and 400 nM PLpro S1*-mutant and incubated for 40 min at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: CVL supernatants were recovered by centrifugation for assessment of IL-8 levels by Enzyme-Linked Immunosorbent Assay (ELISA) (Invitrogen, Paisley, UK), following manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... Proteins were measured by adding 2uL of stock solution (100 nM) to an 8 uL droplet of filtered phosphate buffered saline (Gibco 14190144). Contrast measurements were converted to molecular weights using a standard curve generated with bovine serum albumin (Thermo 23210 ...
-
bioRxiv - Developmental Biology 2021Quote: ... H9 cells were cultured at 37°C and 5% CO2 in feeder-free conditions in Essential 8™ (E8 medium (Gibco, Life Technologies ...
-
bioRxiv - Genetics 2022Quote: ... or one of five Hsa21 genes (100 ng/well; 1 row, 8 wells or technical replicates per unique cDNA) using Lipofectamine 2000 (Invitrogen, 11668030) according to manufacturer’s instructions ...
-
bioRxiv - Pathology 2022Quote: ... The iPSCs were continuously cultured and expanded on Geltrex coated 6-well plates with Essential 8 medium (ThermoFisher Scientific, Cat#A1517001). Human iPSC cell lines used in this manuscript ...
-
bioRxiv - Systems Biology 2022Quote: Cells were cultured and transfected (as described above) for CLSM experiments on 4 or 8-well Lab-Tek™ chambered cover glass slides (ThermoFisher). Before imaging ...
-
bioRxiv - Systems Biology 2022Quote: ... reconstituted in aqueous 0.1% TFA and separated (according to the instructions supplied by the manufacturer) into 8 fractions using the Pierce high pH reversed-phase fractionation kit (Thermo Scientific). For plants 2 (DX ...
-
bioRxiv - Neuroscience 2022Quote: ... 1xPBS was replaced with direct stochastic optical reconstruction microscopy (dSTORM) photo-switching buffer consisting of 50 mM Tris pH 8 solution supplemented with 10 mM sodium chloride (ThermoFisher Scientific), 10% glucose (ThermoFisher Scientific) ...
-
bioRxiv - Immunology 2022Quote: ... The quality/purity of the different purified fractions was evaluated by SDS-PAGE using 3–8% Tris– Acetate gels (Life Technologies) under non-reducing conditions followed by silver staining (Silver Stain kit ...
-
bioRxiv - Immunology 2022Quote: ... The 8-10 μm tissue sections were cut with cryostat (NX70 MicromMicrotech) and we used the Tyramide SuperBoost™ kit (Invitrogen) according to the manufacturer’s instructions for immunofluorescence staining ...
-
bioRxiv - Plant Biology 2022Quote: ... To each sample 0.7 µL of reverse transcription mix (8:2:1:1 of Superscript IV 5X Buffer, 100 mM DTT, RNase Inhibitor, Superscript IV; ThermoFisher Scientific) was added ...
-
bioRxiv - Neuroscience 2022Quote: The tissue slice was rehydrated in PBS at room temperature (RT) (2 × 15 min) and incubated in PBS with 8% SDS (Fisher Scientific) at RT (4 h ...
-
bioRxiv - Cell Biology 2022Quote: ... 2 μg of DNA was used to infect 8 X 105 SF9 cells by using Cellfectin II (Thermo Fisher Scientific; 10362100) two times to increase viral titer to 5 X 106 plaque-forming units/mL ...
-
bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
-
bioRxiv - Cell Biology 2019Quote: ... Cells were then loaded at room temperature for 30 min with fluorescent indicator-linked substrates for activated caspase-8 (10 μM Z-IETD-R110; Molecular Probes), caspase-9 (10 μM Z-LEHD-R110 ...
-
bioRxiv - Biophysics 2019Quote: ... For live cell microscopic analysis, stable DM cells were plated in tomo-dishes (Tomcube, Korea) or Lab-Tek 8-well chambered coverglass (Nunc, USA). For observation of HeLa cells expressing H2B-mRFP ...
-
bioRxiv - Cell Biology 2019Quote: ... Emerging hiPSC colonies were manually picked and cultured under feeder-free conditions in Essential 8 medium on Geltrex-coated dishes (Life Technologies). hiPSC clones were passaged at least 15 times to increase stable pluripotency ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells (INS-1 832/13 and HEK-293T) were seeded onto poly-D-lysine coated 8-well chambered cover glasses (Lab-Tek, Thermo Scientific) at a cell density of 70,000 cells/cm2 ...
-
bioRxiv - Neuroscience 2019Quote: ... seeded at 1.8 × 105 cells/cm2 in plastic plates coated with poly-D-lysine (10 mg/ml) and incubated in Neurobasal (Life Technologies) supplemented with 2 mM glutamine ...
-
bioRxiv - Molecular Biology 2020Quote: ... which were then blotted for approximately 8 s and immediately plunged in 1:2 ethane:propane (Carbagas) at liquid nitrogen temperature using a Vitrobot (Thermo Fisher Scientific). The Vitrobot chamber was kept at 4 °C and 100% humidity during the whole procedure.
-
bioRxiv - Biophysics 2020Quote: The tau cysteine mutants were buffer exchanged into 20 mM NaPi buffer (pH 8) via 7k MWCO Zeba spin desalting columns (Thermo Fisher). 200 μL of 50 μM protein aliquots were reacted with 50 molar equivalents of methyl 2,5-dibromopentanoate (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2020Quote: ... Suspended cells were sonicated for 8 cycles (30s on 30s off) with 30% amplitude on ice using probe sonicator (Sonics vibra cells, Fisher scientific) followed by centrifugation at 9000 rpm for 10 min ...
-
bioRxiv - Bioengineering 2020Quote: ... Neuro-2a cells were seeded into the 8-well chambered coverglass (Nunc™ Lab-Tek™ Chambered Coverglass, Thermo Fisher Scientific) at the density of 15,000 cells/well ...
-
bioRxiv - Genetics 2019Quote: ... 40-50μg of protein was analysed by SDS–polyacrylamide gel electrophoresis (SDS–PAGE) using 3-8% NuPAGE Tris-Acetate protein gels (Life Technologies) and transferred onto nitrocellulose membrane using Xcell Sure Lock Mini Cell electrophoresis tanks (Novex ...
-
bioRxiv - Microbiology 2020Quote: ... The pH was adjusted to pH 8-8.5 with 1 M NaHCO3 and 50 µg of Alexa Fluor 488 NHS Ester (Thermo Fisher Scientific) was added ...
-
bioRxiv - Microbiology 2020Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher cat. A33952), following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... mixed at an 1:1 ratio in a test tube and added to wells of an 8-well slide (Thermo Fisher) coated with poly-lysine (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... Solubilized membranes were mixed 1:1 with binding buffer (20 mM Tris pH 8/1 M NaCl/20 mM imidazole) and loaded onto a HisPur Ni-NTA (Thermo Scientific) column equilibrated with 20 mM Tris pH 8/750 mM sucrose/500 mM NaCl/10 mM imidazole/0.2% DDM (equilibration buffer) ...
-
bioRxiv - Cancer Biology 2020Quote: After 48 hours incubation in the presence of the compounds, cells were fixed by the addition of an equal volume of formaldehyde (8 %, 50 µL) (#28908, Thermo Scientific) to the existing media ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were analyzed by using an 8 capillary ABI 3500 DNA Genetic Analyzer with POP-4 polymer (Life Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... ermGal4) or their unsorted control matches were plated on cover glass (Labtek II Chambered Coverglass, 8-well, 155409, Thermo Fisher Scientific) into Schneider’s medium (Homem ...
-
bioRxiv - Systems Biology 2019Quote: ... Equivalent amounts of protein in the specimen were separated based on their molecular weight by electrophoresis on 8% Bis-Tris gels and transferred to polyvinylidene difluoride (PVDF) membranes (Life Technologies). Membranes were immediately blocked with 5% nonfat dried milk diluted in TBST and incubated with primary antibodies (1:1,000 ...
-
bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then denatured at 95°C for 8 min in the presence of 4X LDS sample loading buffer (Life Technologies) and 5% beta-mercaptoethanol (Bio-Rad ...
-
bioRxiv - Cell Biology 2020Quote: Dried fractions were resuspended in 8 μL 5% ACN + 5% FA and analyzed on an Orbitrap Fusion with in-line Easy Nano-LC 1000 (Thermo Scientific). Fractions were run as 3 h gradients progressing from 3% ACN + 0.125% FA to 100% ACN + 0.125% FA ...
-
bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
-
bioRxiv - Bioengineering 2021Quote: ... All FCS measurements were carried out using 200 µl samples in 8-well chamber slides (Nunc Lab-Tek, Thermo Fisher Scientific). The laser beam was focused 25 µm into the sample for all FCS measurements in this work.
-
bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...
-
bioRxiv - Cell Biology 2021Quote: ... generated from reprogrammed dermal fibroblasts by retrovirus carrying the Yamanaka gene factors) was maintain on Matrigel-coated culture dishes in Essential 8 Flex Medium (ThermoFisher Scientific). The protocols for culturing cell lines and differentiating the hiPSCs into HBs ...
-
bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples were passed through a 25G syringe five times to reduce viscosity and separated on a 3%-8% NuPAGE Tris-Acetate gel (Thermo Fisher). Proteins were transferred onto a polyvinylidene fluoride membrane (Thermo Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... High-resolution mass spectra and MS/MS fragmentation pattern of 1-8 were recorded using a Q Exactive Plus mass spectrometer (Thermo Scientific). Chromatography for determination of amino acyl hydroxamate was performed on a Waters ACQUITY H-class UPLC system coupled to a Xevo TQ-S micro (Waters ...