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Citations for Thermo Fisher :
2101 - 2150 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Total RNA from 8/16-cell embryos (10-15/group/replicate) was isolated using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems). Purified RNA was then subject to reverse transcription with SuperScript II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Tissue was then transferred to 1% fibronectin-coated chambered slides (μ-Slide 8 Well Glass Bottom, Ibidi #80827, for live imaging; Nunc™ Lab-Tek™ II Chamber Slide with removable wells ...
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bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Genetics 2020Quote: ... embryos were washed 8 × 15min in PBST (PBS with 0.1% Tween-20) and incubated in Image-iT FX Signal Enhancer (ThermoFisher Scientific, I36933) for 30 mins at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples of SMG6-3xFLAG in vitro translation reactions were run on 3-8% Tris-Acetate NuPAGE gel (Invitrogen, Cat. No EA03752BOX). Transfer onto nitrocellulose membranes was performed using the iBlot 2 Dry Blotting System (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific, C10337). For induction of apoptosis in myoblasts ...
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bioRxiv - Microbiology 2021Quote: ... was seeded with influent solids harvested from the influent of the RAEBL full-scale WRRF by weekly centrifuging 10 L of a 24-h composite influent sample at 4,000×g using the IEC-Centra-8 centrifuge (Thermo Scientific, USA). The total suspended solids (TSS ...
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bioRxiv - Neuroscience 2020Quote: Human iPSCs were maintained as feeder-free cells in Essential 8 medium on vitronectin-coated plates (all from Gibco, Carlsbad, CA) (69) ...
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bioRxiv - Synthetic Biology 2022Quote: HEK:BFP cells were the kind gift of Chris Richardson.8 Cells were maintained at 37°C and 5% CO2 in DMEM media plus GlutaMax (ThermoFisher Scientific) supplemented with 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 7 days and 14 days post SNI) L3 and L4 DRGs were dissected from 2 mice (8 DRGs/sample) and stored in ice-cold HBSS (Gibco, 14170088). DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase ...
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bioRxiv - Neuroscience 2021Quote: ... or 13C6 L-Lysine-2HCl (Lys-8) and 13C6 L-Arginine-Hcl (Arg-10) for the metabolic incorporation of ‘light’ and ‘heavy’ stable isotopes respectively (ThermoFisher Scientific). HEK293FT cells were grown in light or heavy SILAC media for at least 10 doublings ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were centrifuged again (80 g, 8 min) and suspended at the desired concentrations in culture medium (Neurobasal medium (Thermofisher 21103049) supplemented with 1 % GlutaMAX (Thermofisher 35050) ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate volume was increased to 1 ml and 8 μg of antibody were used in combination with Protein G Dynabeads M-280 (Thermo Scientific) as described above ...
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bioRxiv - Molecular Biology 2020Quote: Flash frozen MCAs with surrounding brain tissue were sliced to 8 μm sections using a cryotome (Fisher Scientific, Pittsburgh, PA, USA), placed on charged slides (2-4 slices/slide) ...
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bioRxiv - Molecular Biology 2020Quote: INS1 cells were re-plated onto a cover slip as above and incubated in regular INS1 medium containing 8 μM Bodipy C12 (Molecular Probes) at 37 °C at 5% CO2 for the last 16 h of culture ...
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bioRxiv - Molecular Biology 2020Quote: ... The cells were transfected with indicated plasmids alone or in combination for 8 h by using Lipofectamine 3000 (Thermo Fisher, USA), and then continue to cultivate for 24 h in the fresh medium before being subjected to indicated experiments.
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bioRxiv - Molecular Biology 2020Quote: ... and 10-20 µg of protein loaded on a 3%–8% NuPAGE Tris-Acetate Gel in tris-acetate buffer (Novex Life Technologies) or a 4-12% NuPAGE TrisBis NuPAGE gels in MES buffer (Novex Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Each CDM pellet was resuspended in 100 mM NH4HCO3 solution containing 8 M urea and 10 mM dithiothreitol (DTT; Thermo Fisher) and incubated at 37 °C for 2 hours under agitation ...
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bioRxiv - Cell Biology 2020Quote: ... 25 µL drops of cell-Matrigel suspensions were seeded into each well of an 8-well chambered coverglass (Nunc, No. 1.5) and incubated at 37 °C for 30 minutes before adding DMEM to cells ...
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bioRxiv - Immunology 2021Quote: ... and checked for purity and quality on a silver-stained SDS-PAGE gel (3-8% Tris-Acetate Novex, Thermo Fisher Scientific). The anti-NTD neutralizing antibody NTD-18 and the pan-coronavirus anti-S2 non-neutralizing antibody Ab-10 were previously described 9,10.
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bioRxiv - Cancer Biology 2019Quote: ... HEK293T cells in a 10 cm dish with about 60% confluence were transfected with 8 μg lentiviral construct and helper plasmids using Lipofectamine 2000 (Life Technologies). 24 hours after transfection ...
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bioRxiv - Cell Biology 2019Quote: ... for NIH-3T3 cells and P19 cells or 5nM siRNA/8 µl INTERFERin (PolyPlus) for MDA-MB-231s and 200 µl Opti-MEM (ThermoFisher Scientific) in fresh media ...
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bioRxiv - Cancer Biology 2019Quote: ... LDHA proteins in tumor extract were separated on 4% to 20% Tris-Glycine gel and CD31 was separated on NuPAGE 3 to 8 % Tris-Acetate gel (Life Technologies) by SDS-PAGE and were transferred to nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2019Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA produced from 15 ng RNA and 8 pmol of each forward and reverse primer per reaction and Power SYBR Green PCR Master Mix (Applied Biosystems) were used ...
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bioRxiv - Pathology 2019Quote: PMLECs between the 3rd and 5th passages were seeded (8 × 104/well) in 2-well Permanox chamber slides from Lab-tek (Thermo Scientific, Nunc). The cells were incubated at 37°C and 5% CO2 for 24 hours ...
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bioRxiv - Genomics 2020Quote: ... 1% Triton X-100 and 10% glycerol and resolved on 3–8% pre-cast gradient Tris-Acetate gels (Invitrogen, Waltham, MA). For mouse samples ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were harvested before the cultures reached OD600 = 1 and were labeled with 8 mg/ml EZ-Link Sulfo-NHS-LC-biotin (ThermoFisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Systems Biology 2019Quote: ... Human ESCs (WA-01; National Institutes of Health registry #0043) were maintained in Essential 8 medium (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin-amphotericin B solution (Lonza ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The homogenate was centrifuged at 10,000g for 15 minutes (Sorvall™ ST 8 Small Benchtop Centrifuge: Thermo Scientific, Waltham, MA, USA). The supernatant was used as the enzyme source ...
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bioRxiv - Molecular Biology 2019Quote: Human atherosclerotic plaque or mouse aortic root specimens were first cut into 8 μm sections using a freezing microtome (Thermo Scientific). For immunofluorescence detection to detect Tf ...
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bioRxiv - Developmental Biology 2019Quote: ... One-third to one embryo equivalents were loaded at per lane on 4%-12% or 8% Bolt® Bis-Tris (Invitrogen). GFP Rabbit IgG Polyclonal Antibody (Molecular Probes ...
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bioRxiv - Biophysics 2019Quote: ... were plasma-cleaned for 10 minutes in an Harrick Plasma Cleaner (Harrick) and attached to 8-well LabTek chambers (Nunc, USA), for which the bottom glass slide had been removed ...
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bioRxiv - Genetics 2019Quote: Xenograft mouse model of human lung cancer tumors were implanted under the left upper limb in the abdomens of 6- to 8-week old male NCG mice by subcutaneous injection of A549 (5×106 cells in 200 µL DPBS (Gibco, C14190500BT)) or H2228 cells (2× 106 cells in 200 µL DPBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were resolved for 4 hr at 100 V on 4% −8% polyacrylamide gradient Tris-acetate denaturing gels (Novex, Life Technologies) and transferred to PVDF membranes ...
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bioRxiv - Plant Biology 2020Quote: ... Blocks were sectioned using a rotary microtome to 8 μm thickness and then floated on Arcturus PEN membrane slides (Fisher Scientific) with DEPC-treated water ...
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bioRxiv - Immunology 2020Quote: ... Peptides (8 μl) were loaded using a Dionex Ultimate 3000 nano-flow ultra-high-pressure liquid chromatography system (Thermo Scientific, USA) on to a 75µm × 2 cm C18 trap column (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Confluent cells were first differentiated overnight with serum-free DMEM in 8-well chamber slides (Nunc Lab-Tek Chamber Slide System, Thermo Scientific). Cells were then washed and treated with anesthetics or other drugs for 10 min ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... The lysate was cleared by centrifugation at 12,000 × g at 4 °C for 40 min in a Fiberlite TM F15-8 × 50 cy Fixed Angle Rotor (ThermoFisher Scientific). The resulting lysate was filtered through a 0.45 μm syringe filter and loaded onto a 5 ml HiTrap™ TALON Crude cassette (GE ...
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bioRxiv - Microbiology 2021Quote: ... ExpiCHO cells were cultured in a shaker incubator at 33 °C at 115 rpm with 8% CO2 according to the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13 to 14 days after transfection ...
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bioRxiv - Microbiology 2021Quote: ... and 2×105 cells were seeded into 24-well culture plates or 5×104 cells into Lab-Tek II 8-well chamber slides (Nunc/Thermo Fisher). To account for donor variations ...
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bioRxiv - Neuroscience 2020Quote: ... 8 µg of the cDNA from each sample were fragmented and labelled with GeneChip Mapping 250K Nsp assay kit (Affymetrix, 900753). Hybridization was performed as described previously31 during 16 h at 45°C ...
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bioRxiv - Cell Biology 2021Quote: ... Embryos older than 24 hpf were permeabilized in 10 μg/mL Proteinase K solution (8 min for 24 hpf, 28 min for 48 hpf, 30 min for 5dpf and 7dpf embryos) (Invitrogen, 25530015). ISH was then performed as described previously (Thisse and Thisse ...
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bioRxiv - Developmental Biology 2021Quote: ... Coriell) were maintained under feeder-free conditions using Essential 8 (E8) Medium and vitronectin (VN)-coated plates (#A14700; Thermo Fisher Scientific). Cells were routinely passaged using 0.5 mM EDTA diluted in phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... with a mu- phosphatase signal sequence and a C-terminal Avi-8xHis-EPEA-tag in a pD2610-V5 vector (ATUM Bio) were expressed in Expi293F cells at 37°C and 8% CO2 according to manufacturer’s instructions (Thermo Fisher Scientific). Cell culture supernatant was collected after four days and purified over a 5 mL C-tag affinity matrix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: Human induced pluripotent stem cells (hiPSCs) were maintained in feeder-free monolayers on Geltrex (ThermoFisherScientific) and fed daily with Essential 8 medium (Life Technologies). When confluent ...
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bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...
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bioRxiv - Bioengineering 2020Quote: FACS-purified CD3-CAR+ or CD3-CAR- CD4+ T cells (8 x 106 each) were lysed in Pierce™ IP Lysis Buffer (ThermoFisher) supplemented with Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Bioengineering 2020Quote: iPSCs (DF19-19-9-11T) purchased from WiCell Technologies were maintained on vitronectin-coated plates in Essential 8 medium (E8, ThermoFisher Scientific), and passaged when the colonies reached ~75-80% confluence ...