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Citations for Thermo Fisher :
2151 - 2200 of 2828 citations for 8 Benzoyloctanoicacid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 5 % of the input and all of the output volume were loaded on an SDS-PAGE using 8-12 % Novex Wedge Well Tris-Glycine gel (Thermo Scientific). The gel was then imaged on Amersham Typhoon (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μg protein/lane was then separated under denaturing conditions on reducing 3~8% Tris-Acetate gels (NuPAGE, Thermo Fisher Scientific), and HiMark protein standard (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Neuroscience 2021Quote: ... All iPSC lines were maintained on Vitronectin-coated plates and fed with Essential 8 (E8) + E8 supplement media (ThermoFisher, CAT#: A1517001).
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bioRxiv - Neuroscience 2021Quote: ... 20 μg of each sample was separated in 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes (45-004-110, Fisher Scientific) in ice-cold transfer buffer (25 mM Tris-base ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were mounted on glass microscope slides using 8-10 µl of Prolong Gold Antifade mounting media with DAPI (Invitrogen, P36941) per coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... and ESC (WiCell H9 WA09, H7 WA07) lines were maintained on feeder free conditions on Essential 8 media (E8, Life technologies) and Geltrex (Life technologies ...
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bioRxiv - Immunology 2021Quote: ... ExpiCHO cells were cultured in a shaker incubator at 33 °C at 115 rpm with 8% CO2 according to the Max Titer protocol with an additional feed on day 5 (Thermo Fisher). Culture supernatants were harvested 13-14 days after transfection ...
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bioRxiv - Cancer Biology 2021Quote: 3D breast acinar cultures were set up in an 8-well chamber cover glass plates (Nunc Lab-Tek, Thermo Fisher Scientific) or 12-well plates (Eppendorf ...
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bioRxiv - Cancer Biology 2021Quote: 3D breast acinar cultures were set up in an 8-well chamber cover glass plates (Nunc Lab-Tek, Thermo Fisher Scientific) or 12-well plates (Eppendorf ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained with primary antibodies: cytokeratin-8 (1:100; TROMA-I, DSHB) and cytokeratin- 14 (1:250; Invitrogen, PA5-16722) and with species-specific fluorochrome-conjugated secondary antibodies (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... Bead-binding was performed using 30 pl of Protein G Dynabeads (Thermo Fisher Scientific Baltics UAB, V.A. Graiciuno 8, LT-02241 Vilnius, Lithuania. Thermo Fisher Scientific Baltics UAB complies with Quality System Standards ISO 9001 and ISO ...
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bioRxiv - Cell Biology 2021Quote: ... Cells from both hippocampi of each mouse were pooled together and plated in 2 wells of an 8-well Chambered Coverglass (ThermoFisher, 155409PK) at a density of ~250,000 cells per chamber ...
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bioRxiv - Neuroscience 2020Quote: ... with titan rotor T29-8×50 in oak ridge centrifuge tubes with sealing caps (all from Thermo Fisher Scientific, Rochester, NY). After removal of the supernatants ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... For analysis of mitochondrial fragmentation cells were plated at 20,000 per well in NuncTM Lab-TekTM II Chambered Coverglass (8 well, 155409, Thermo Fisher Scientific).
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bioRxiv - Physiology 2020Quote: ... Upon achieving confluence, they were treated with 8 μM GSK3 inhibitor (CHIR99021, Stemgent) in RPMI supplemented with B27 lacking insulin (Thermo Fisher). 24 hrs later ...
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bioRxiv - Physiology 2020Quote: All 7 gills from each individual shrimp (5 ≤ N ≤ 8) were dissected under a magnifying lens with micro-scissors and homogenized in TRIzol reagent (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... 8 mg of the soluble protein fractions were pre-cleared with 200 μL of protein G-magnetic Dynabeads (Thermo Fisher Scientific) for 1 hr ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were denatured by adding 4xLDS buffer and heating at 70°C for 10 min before being separated in 3-8% Tris-acetate gels (Thermo Fisher) run at 150 V for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Plates were blocked on the following day for 1hr with TBS-TMS (20mM Tris pH 8, 150mM NaCl, 0.05% Tween 20, 1% non-fat dry milk, 1% goat serum (Gibco 16210-072)) ...
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bioRxiv - Cell Biology 2021Quote: ... The pooled samples were then fractionated into 8 fractions using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific 84868), then dried by speed-vac ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were then resolved on 4-12% Bis-Tris gels (for lower molecular weight proteins) or 3-8% Tris-Acetate gels (for higher molecular weight proteins) (NuPage, Thermo-Fisher Scientific) and run in MOPS or Tris-Acetate running buffer respectively ...
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bioRxiv - Microbiology 2020Quote: ... Samples were DNAseI treated and cDNA was generated from 8 μL of purified RNA/individual (∼500 ng of RNA) using SuperScript™ III First-Strand Synthesis System (ThermoFisher). cDNA was diluted 2-fold with RNase-free water then expression levels of selected immune genes was determined by amplifying 1 μL of cDNA with primers for cecropin D (AAEL000598 ...
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bioRxiv - Microbiology 2021Quote: ... The vesicles were formed encapsulating the fluorescent membrane-impermeable dye 8-hydroxypyrene-1,3,6-trisulfonic acid from Thermo Fisher (HPTS, 50 μM). The microfluidic platform was operated by two positive pressure-driven pump modules (MFCS-4C ...
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bioRxiv - Systems Biology 2021Quote: ... at 40°C using a linear gradient of 120 minute shallow gradient rising from 8 % (v/v) acetonitrile/0.1 % (v/v) formic acid (Fisher Scientific, UK) to 30 % (v/v ...
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bioRxiv - Systems Biology 2020Quote: ... the differentiated cardiomyocytes were re-plated in Nunc™Lab-Tek™II glass-bottomed 8-chamber glass slides (Thermo Fisher), and then cells were fixed and permeated in the plate using a Human Cardiomyocyte Immunocytochemistry Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were extracted using lysis buffer (8 M urea, 2 M thiourea, 4% CHAPS, 40mM DTT) supplemented with Halt protease inhibitor complete cocktail (Thermo Fisher) by bead beating (50 Hz for 3 five-minute cycles ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were assembled in either 8-tube Genie strips (OP-00008, OptiGene Ltd) or 96-MicroAmp-Fast-Optical reaction plate (Applied Biosystems). Strip tubes reactions were capped ...
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bioRxiv - Microbiology 2020Quote: ... EBV producer HEK-293 cells stably transformed by BART-repaired B95-8 based EBV BAC system encoding GFP (45) were cultured in RPMI 1640 (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... GFP antibody and HER2/neu Ab-8 (Clone e2-4001) mouse monoclonal antibody used for IP were obtained from Thermo Fisher. C6orf97 antibody is from GeneTex ...
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bioRxiv - Cell Biology 2021Quote: ... and the pellets were dissolved in 0.1% SDS/ 0.67 M Tris-HCl pH 8 +/- 15 mM 4-acetamido-4’-maleimidylstilbene-2,2’-disulfonic acid (Thermo Fisher Scientific, USA). The samples were incubated at 37 °C for 2 h ...
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bioRxiv - Cell Biology 2020Quote: ... 14 mM Glucose and 4 mM L-AlanylGlutamine supplemented with 8% heat-inactivated fetal bovine serum (FBS) (#10082139; Thermo Fisher Scientific), EGF 5 ng/ml (#01-107 Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Genomics 2021Quote: ... and 0,8 mg/ml BME V2 were added in a volume of 50µl per well using a Multidrop dispenser (Thermo Fisher Scientific). Plates were sealed with a plate-loc (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2022Quote: Protein extracts were resolved using either home-made SDS-PAGE gels or 3-8% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific), when analysing proteins of a molecular weight >180 kDa ...
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bioRxiv - Biochemistry 2022Quote: ... At culture day 8 cytosolic Ca2+ levels in primary neurons were determined using the Ca2+-sensitive fluorescent indicator Fura-2-AM (Molecular Probes/Invitrogen). Cells were loaded with Fura-2 in sterile-filtered HEPES-buffered saline (HBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Viral supernatant was collected and concentrated after 48 hours and transduced directly into hiPSCs at low to mid-confluency along with 8 μg/mL polybrene (Fisher Scientific). Because all plasmids contained puromycin-resistance cassettes ...
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bioRxiv - Neuroscience 2022Quote: ... GFP+/Luc+ NPCs were diluted with a final concentration of 8 × 104 cells/μL in sterile 1x PBS (pH 7.4, without calcium or magnesium; Thermo Fisher Scientific) and stored on ice until transplantation ...
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bioRxiv - Biochemistry 2022Quote: Exosomes from mice plasma were harvested from age matched WT and SR-/- mice (6-8 weeks) plasma using the total exosome precipitation reagent for plasma (Invitrogen #4484451) as per instructions ...
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bioRxiv - Biochemistry 2022Quote: ... using a 10-minute 8-38% acetonitrile gradient (200 ml/min) and subsequent MS2 by HCD on a Fusion Lumos mass spectrometer (ThermoFisher Scientific). Phosphorylated residues (serine ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
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bioRxiv - Neuroscience 2022Quote: ... then the flow-throughs were fractionated into 8 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... and 8×104 cells were plated into 96-well U-bottom and activated with anti-CD3/anti-CD28-coated Dynabeads (Thermo Fisher), as previously described89 ...
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bioRxiv - Pathology 2022Quote: ... Protein concentration was determined with Bio-Rad Protein Assay Kit II and 15 μg sample protein was loaded in a NuPAGE 3-8 % Tris-Acetate gel (Thermo Fisher). The gel was run at a constant voltage of 150 V for 4 h ...
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bioRxiv - Microbiology 2022Quote: ... mutations were inserted in fragment 8 (Supplementary Table 4) using the GENEART site-directed mutagenesis system (Thermo Fisher Scientific, Cat# A13312) according to the manufacturer’s protocol with the primers listed in Supplementary Table 5 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were maintained at 37°C with 5% CO2 with daily replacement of Essential 8 media system (Thermofisher, Canada. Catalog#A1517001). Cells were passaged as aggregates every 3-4 days until they reach 60-70% confluency with 0.5mM EDTA diluted in PBS (Thermofisher ...
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bioRxiv - Microbiology 2022Quote: ... HCT-8 cells were cultured in 25 cm flasks with 5 ml of complete media (RPMI-1640 media [Gibco/Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... Purified surface proteins or total cell lysates were separated on a NuPage 3-8 % Tris-acetate protein gel (Thermo Fisher Scientific) at 200 V for 40 min and transferred to a PVDF membrane ...