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Citations for Thermo Fisher :
2301 - 2350 of 8618 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... proteins were dialyzed overnight using a 7 or 10 kDa molecular weight cut-off Slide-A-Lyzer dialysis cassette (Catalog No. 66380, Thermo Scientific) in high salt buffer (500 mM NaCl and 20 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR reactions (all samples are run in triplicate) were carried out on the QuantStudio™ 7 Flex system (Applied Biosystems), and results were calculated by the delta-delta Ct (ΔΔCt ...
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was run in triplicates using 2-5 ng cDNA in a 384 well plate and the qPCR was performed using VIIA 7 real time PCR instrument from Applied Biosystems.
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bioRxiv - Cancer Biology 2023Quote: Cell viability in untreated tumor organoids was analyzed on day 7 using the LIVE/DEADTM Cell Imaging Kit (Invitrogen, Karlsruhe, Germany) according to the manufactureŕs instructions for exemplary cases.
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Genomics 2023Quote: The RT-qPCR reactions were carried out on 384-well plates using a QuantStudio 7 Flex Real- Time PCR System (ThermoFisher #4485701) using 10 µl reactions (2 µl of cDNA 3.7 ng/µl ...
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bioRxiv - Plant Biology 2023Quote: Total protein isolated from the light-grown 7-day-old seedlings of each sample using a plant protein extraction kit from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Cell Biology 2023Quote: ... BAL samples were then centrifuged at 3,000 g for 20 min to collect cells and ApoBDs for staining with a combination of SR-DEVD-FMK (caspase 3/7 activity detection dye, Thermo Fisher), annexin A5-V450 and TO-PRO-3 in 1× A5 binding buffer at room temperature for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: 500,000 MDA-MB-231 cells plated in a six-well dish were transfected with 20 nM of the appropriate siRNA (IDT) or 20 nM of appropriate LNA diluted in OPTI-MEM containing 7 µL of RNAiMAX (Thermo Fisher). Transfections were performed according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The medium was changed every day and cells were passaged at 70%-80% confluency every 5-7 days using PBSCa-/Mg- (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: qPCR was performed with 2.5 ng of DNA in triplicates using PowerSYBR Green PCR master mix at Quant Studio 7 qPCR machine (both Applied Biosystems) according to the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... Hippocampi of male and female E17 mouse brains from a single litter (7-9 embryos) were dissected in HBSS (ThermoFisher Scientific) supplemented with 1% L-glutamine ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... The qPCR experiments were performed in triplicate in a ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Proliferation was assessed 7 days after treatment with respective drugs using CellTiter-Glo® luminescent Cell Viability Assay (Fisher Scientific, PRG9242). The luminescence signal was measured in a luminometer ...
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bioRxiv - Developmental Biology 2023Quote: ... or Blue ŚGreen qPCR kit (Byozim) on a 7900HT Real Time PCR system or QuantStudio 7 Flex Real-Time-PCR-System (Applied Biosystems). Primer sequence information is provided in supplementary table 4 ...
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bioRxiv - Biophysics 2023Quote: ... The cell-culture medium contained a 1:1 mixture of Eagle’s minimal essential medium (EMEM; for MCF-7) and F12 (Invitrogen, Waltham, MA) with a 2% B-27 supplement (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... beads were resuspended in 20 µl of Elution Buffer mixed with 7 µl of 4X LDS sample buffer (Thermo Fisher, # NP0007) and 3 µl of 10X reducing agent (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... cells were reverse-transfected into an arrayed 384- well plate library containing small interfering RNA (siRNA) or miRIDIAN microRNA mimic constructs (Horizon Discovery, Supplementary Tables 1-7) using Opti-MEM (Gibco 31985070) and Lipofectamine RNAiMAX transfection reagent (Invitrogen 13778150) ...
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bioRxiv - Molecular Biology 2023Quote: ... Movies were collected on a Titan Krios G3i at spot size 7 with a Falcon 4 Direct Detector (Thermo Fisher Scientific) equipped with an energy filter set to 20 eV ...
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bioRxiv - Immunology 2024Quote: ... and normal tissues were mechanically cut into 3-7 mm fragments and incubated in RPMI 1640 medium (Gibco™, cat. 42401042) containing 1 mg/ml Liberase TL Research Grade (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... dense cultures containing organoids ranging in size from 100 to 500 µm were passaged every 7-10 days with TrypLE (Life Technologies) containing 10 mM ROCK inhibitor and 10 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: ... qRT-PCR was performed using TaqMan Fast Advanced Master Mix and predesigned TaqMan probes on a 384-well plate using the ViiA 7 Rea-time PCR system (Thermo Fisher). The predesigned TaqMan probes used are as follows ...
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bioRxiv - Cancer Biology 2024Quote: Nuclear and cytoplasmic fractions of MCF-7 were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Kit (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... Formation of spheroids was induced by seeding MCF-7 cells at 750–1250 cells/well in 96-well U-bottomed ultra-low attachment plates (174929, Life Technologies) with shaking ...
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bioRxiv - Biophysics 2024Quote: ... Native PAGE or SDS PAGE gels were transferred to a nitrocellulose membrane using an iBlot2 (20 V, 7 min; Invitrogen™). The membrane was incubated with blocking buffer (PBS ...
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bioRxiv - Microbiology 2024Quote: ... the samples were stained with 80 μL CellEvent Caspase 3/7 Green detection Reagent 2 µM in PBS with 5% FBS (Invitrogen, C10423) and then incubated for 30 minutes at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were then differentiated into macrophages by 7–9 days of culture in IMDM with glutamax base + 10% AB human serum (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: ... COS-7 cells were transfected with reporter plasmid construct together with pRL-TK Renilla luciferase plasmid by Lipofectamine™ 2000 (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... 100 reverse transcription products and standard plasmids were subjected to real-time PCR analysis (QuantStudio 7 Flex Real-Time PCR System; Applied Biosystems) using human β-actin as an internal control ...
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bioRxiv - Molecular Biology 2024Quote: Human embryonic kidney cell line HEK293FT and breast cancer cell line MCF-7 were both cultivated in Roswell Park Memorial Institute medium (RPMI, Thermo Fisher Scientific, Gibco) supplemented with 10% FCS (fetal calf serum ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were maintained at 37°C and 5% CO2 and dissociated using 0.25% trypsin/1 mM EDTA (WISENT 325-043-EL) for 7 min after one wash with 1x DPBS without Ca2+ and Mg2+ (Gibco 14190250) for SCC-13 cells or 3 min for all other cells ...
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bioRxiv - Cell Biology 2024Quote: The mouse macrophage cell line RAW264.7 (ATCC, VA, USA) was cultured in dulbecco’s modified eagle medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, CA, USA) at a 37°C and 5% CO2 incubator ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 μl RNA were used in each PCR reaction using KAPA SYBR FAST One kit (KK4651) and run on a QuantStudio 7 Flex System (Applied biosystems).
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bioRxiv - Neuroscience 2024Quote: Primary cortical neurons were maintained for 7 days (DIV 7) before EV treatment at 37°C in 5% CO2 and cultured in serum-free Neurobasal medium (Life Technologies) supplemented with 1.2% GlutaMAX-I (Life Technologies) ...
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bioRxiv - Physiology 2024Quote: ... The mRNA expression levels were determined by real-time quantitative RT-PCR using an Applied Biosystems (QuantStudio 7 Flex, Thermofisher, USA). The sequences of specific primers are listed in Supplemental Table 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenyl-indole (DAPI, Life Technologies) and sections were examined with a confocal laser scanning microscope (Carl Zeiss Inc ...
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bioRxiv - Plant Biology 2020Quote: The procedure for cloning KDR CDS was as described before but with the use of the primers listed in Supplemental Table 7 and cloned into the Gateway vector pDONR221 (Thermo Fisher Scientific). The competent cells transformed with the entry vectors were selected for growth on the antibiotic kanamycin (50 µg/ml) ...
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bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The cDNA was further used for Real-time quantitative polymerase chain reaction (qPCR) performed on QuantStudio 7 Flex system (Invitrogen™ Life Technologies) using Fast SYBR Green Master Mix (Life Technologies).
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bioRxiv - Cell Biology 2020Quote: Gene expression analysis was performed by quantitative real-time RT-PCR (qRT-PCR) using SYBR Green PCR Master Mix and the ViiA™ 7 Real-Time PCR System (Applied Biosystems). For each target gene ...
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bioRxiv - Immunology 2021Quote: ... 1:100 human IgG (1 mg/ml) as FcR block and 2 % FCS using the following staining reagents: 7-AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Immunology 2021Quote: ... Antibody was harvested from the cell supernatant after 5 to 7 days post inoculation and purified using Protein L Sepharose beads (Thermo Fisher Scientific).