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Citations for Thermo Fisher :
2251 - 2300 of 8618 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... Gene expression was quantified with an SYBR Green real time-PCR using the ViiA™ 7 system (4453535, Thermo Fisher Scientific). β-Actin was used as a housekeeping gene to normalize the amounts of cDNA in each sample ...
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bioRxiv - Neuroscience 2024Quote: ... were removed from the culture membrane AT 7 DIV with a scalpel blade into pre-filled bead mill tubes (Fisherbrand: 15555799) containing RNAlater stabilisation solution (ThermoFisher: AM7020) and kept at 4°C until RNA extraction ...
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bioRxiv - Neuroscience 2024Quote: Intracellular ROS in BV-2 cells was measured by incubating cells with 1 µM cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen, D399) at 37°C for 30 min.
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with solution containing 468 μl of 50 mM ammonium bicarbonate and 7 μg of Trypsin (Thermo Fisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
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bioRxiv - Developmental Biology 2024Quote: ... Real-time PCR amplification and fluorescence detection were carried out using a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher). An initial step (95 °C ...
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bioRxiv - Genomics 2024Quote: ... was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system using standard settings (Applied Biosystems). Expression of genes was normalised to two housekeeping genes (Bactin ...
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bioRxiv - Molecular Biology 2023Quote: 500,000 MDA-MB-231 cells plated in a six-well dish were transfected with 20 nM of the appropriate siRNA (IDT) or 20 nM of appropriate LNA diluted in OPTI-MEM containing 7 µL of RNAiMAX (Thermo Fisher). Transfections were performed according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... The qPCR experiments were performed in triplicate in a ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... or Blue ŚGreen qPCR kit (Byozim) on a 7900HT Real Time PCR system or QuantStudio 7 Flex Real-Time-PCR-System (Applied Biosystems). Primer sequence information is provided in supplementary table 4 ...
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bioRxiv - Cell Biology 2022Quote: ... Single-axis tilts were collected from −60° to +60° at 2° increments at 7 □µm defocus on a Falcon 3 camera (Thermo Fisher) operated in linear mode at 22,000X magnification ...
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bioRxiv - Microbiology 2023Quote: ... Proteins RNAylated with Cy5-labelled RNA were equilibrated in the same buffers using Zeba Spin desalting columns (7 MWCO, 0.5 ml) (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: Total RNA from the whole bodies (four insects in each biological replicate/sample) and from MT (7-10 MTs in each sample/biological replicate) was isolated with TRIzol (Invitrogen, 15596026). Isolated RNA samples were treated with DNase I (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: Gene expression of targets listed in supplemental table 2 were analysed by SYBR green-based qPCR analysis using Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Thermo Scientific), following the standard PCR cycling sequence using GAPDH as an internal control ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR was performed using SYBR green Advanced Master Mix and run on a ViiA 7 Real-Time PCR System (all from Applied Biosystems). Primers were in-house designed (see table 1 ...
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bioRxiv - Microbiology 2023Quote: ... plated at a density of 7 x 106 cells in a 175 cm2 tissue culture flask in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Pathology 2023Quote: ... The mRNAs were quantified using individual TaqMan assays described in Supplemental Table 2 on an ABI QuantStudio 7 Real-Time PCR machine (Applied Biosystems) using TaqMan Fast Advanced Master Mix (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR program comprised an initial denaturation for 30 s at 95 °C and amplification by 40 cycles of 15 s at 95 °C and 1 min at 60 °C and was ran in an ABI QuantStudio 7 Real Time PCR machine (Applied Biosystems). Expression values of DIN6 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was reverse transcribed with qScript cDNA supermix (Quanta Bio, #95048-500) and qRT-PCR was performed with QuantaStudio 7 Real-time qPCR System (Thermofisher, #A43183). Transcript levels of Htr2c ...
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Early life lipid overload in Native American myopathy is phenocopied by stac3 knock out in zebrafishbioRxiv - Genetics 2023Quote: ... Gene expression was quantified using the PowerUp SYBR Green Master Mix and a ViiA™7 Dx qPCR Instrument (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... and 0.1 mM AMPPNP) using a 0.5-mL Zeba spin desalting column (7-kD molecular weight cutoff, Thermo Fisher Scientific, #89882). AMP-PNP and Taxol were added to the flow-through to final concentrations of 1 mM and 10 µM ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time PCR reactions (TaqMan and SYBR Green) were performed on a ViiA 7 Real-Time PCR System (Applied Biosystems). For quantitative real-time PCR of selected TFs ...
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Molecular Biology 2023Quote: SGBS cells in the undifferentiated state or on day 7 of differentiation were electroporated using the Neon™ Transfection System (Invitrogen) with C-terminally tagged target genes or GFP control ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was purified from the same cultures after 7 hours of growth using GeneJET RNA Purification Kit (Thermo Fisher Scientific) and analyzed in the same way ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Zoology 2023Quote: ... kit and sequenced via ABI 3730XL DNA sequencer platform using the BigDye Terminator (version 3.1) Cycle Sequencing Kit and POP-7 Polymer separation matrix (Applied Biosystems, Inc.) using both forward and reverse primer ...
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bioRxiv - Developmental Biology 2023Quote: ... and qPCR for Sfrp2 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems). Primers used for qPCR are listed in Extended Data Table 2.
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bioRxiv - Cell Biology 2023Quote: ... They were single cell dissociated and 7 days later RNA was extracted using the Arcturus PicoPure RNA extraction kit (Thermofisher, KIT0204) according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Samples were incubated on grids for 7 seconds before being blotted off and plunge-frozen in liquid ethane using a Vitrobot mark IV (Thermo Fisher).
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bioRxiv - Molecular Biology 2023Quote: ... the lysate equivalent to OD (A260) = 7 was subjected to RNase I digestion ( 5U per OD, 35U in total; Invitrogen, AM2294) at 22 °C/ 20 minutes with continuous rotation at 1000 rpm in thermoblock (Eppendorf) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained at 37°C for 30 min with 2 µM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCF-DA; Thermo Fisher Scientific) or 1 µM MitoSOX (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Library concentration was normalized to 2 nM and concentrations validated by qPCR on a ViiA-7 real-time thermocycler (Applied Biosystems), using qPCR primers recommended in Illumina’s qPCR protocol and Illumina’s PhiX control library used as a standard ...
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bioRxiv - Biophysics 2023Quote: ... the media was removed and the cells were incubated with 8 µM CellEventTM Caspase 3-7 green detection reagent (Thermo Fisher) in PBS containing 5% FBS for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: FOXM1 pmirGLO plasmid along with miR-4521 mimic and scrambled negative control oligo were co-transfected into MCF-7 breast cancer cell lines using Lipofectamine 3000 reagent (Invitrogen, USA), according to the manufacturer’s instructions (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... 0.8-2μg of RNA were reverse transcribed using the High-Capacity cDNA reverse transcription kit and quantitative real-time PCR was carried out with the ViiA 7 Real-time PCR system (both Applied Biosystems). qPCR Mastermix (PrimerDesign ...
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bioRxiv - Cell Biology 2022Quote: Lysosomal mass was estimated by incubating cells under various genetic perturbations (7 days after sgRNA transduction) with LysoTracker Red DND-99 at 100 nM (Invitrogen; L7528). After incubation at 37ºC for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl was loaded on to NuPAGE 4 to 12% Protein Gels with 7 μl of 4X NuPAGE LDS sample buffer as described by the manufacturer (Invitrogen: NP0322BOX) and run for 2 h at 100 V ...
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bioRxiv - Neuroscience 2023Quote: ... and SARS-CoV-2 N1 primers/probe mix (Stock# 10006713; Integrated DNA Technologies) were run on a ViiA 7 Real-Time PCR system (Applied Biosystems) as described in the instructions for use of the CDC 2019-Novel Coronavirus (2019-nCoV ...
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bioRxiv - Neuroscience 2023Quote: Third instar larval brains were dissected in phosphate buffered saline (PBS) and fixed with 4 % formaldehyde (FA) pH 7 (Thermo Scientific)/PBS for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: Real-time PCR was performed by standard TaqMan Assay on either the QuantStudio 7 or Quantstudio 5 Real-Time PCR platform (Applied Biosystems). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... Following EDTA dissociation crypts were incubated at 37°C for 7 minutes in TrypLE Express (Thermo Fisher Scientific, 12-604-021) and DNase I (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... proteins were dialyzed overnight using a 7 or 10 kDa molecular weight cut-off Slide-A-Lyzer dialysis cassette (Catalog No. 66380, Thermo Scientific) in high salt buffer (500 mM NaCl and 20 mM Tris ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Microbiology 2023Quote: ... RT-PCR reactions (all samples are run in triplicate) were carried out on the QuantStudio™ 7 Flex system (Applied Biosystems), and results were calculated by the delta-delta Ct (ΔΔCt ...
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: Colon crypts from 4 to 7 months old mice were isolated as described above and then resuspended in 4 ml of TryplExpress (ThermoFisher Scientific) for 15 min at 37°C under agitation at 75 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was run in triplicates using 2-5 ng cDNA in a 384 well plate and the qPCR was performed using VIIA 7 real time PCR instrument from Applied Biosystems.
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bioRxiv - Cancer Biology 2023Quote: Cell viability in untreated tumor organoids was analyzed on day 7 using the LIVE/DEADTM Cell Imaging Kit (Invitrogen, Karlsruhe, Germany) according to the manufactureŕs instructions for exemplary cases.