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Citations for Thermo Fisher :
2351 - 2400 of 8618 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Blocks were later cut on a cryostat and 7-10 μm thick sections were placed on SuperFrost Plus slides (Cat#FT4981GLPLUS; ThermoFisher; Waltham, MA). Hematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Genetics 2021Quote: ... The purified products were separated and detected by capillary electrophoresis (CE) using the ABI 3130 Genetic Analyzer with POP-7 polymer and GeneScan-120 Liz (Thermo Fisher Scientific). The raw data were analyzed using the GeneMapper ID V3.2 software (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2021Quote: ... cyli kidneys was determined by quantitative real-time PCR performed on a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific) using the default program ...
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bioRxiv - Molecular Biology 2020Quote: ... was added to bind excess dye and the resultant solution was passed through 7 K MWCO Zeba Spin Desalting Columns (Thermo Fisher Scientific) to remove excess dye ...
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Cancer Biology 2020Quote: ... All qRT-PCR experiments were run on the ViiA™ 7 Real-Time PCR System (Life Technologies, Thermo Fisher Scientific, Waltham, MA)
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bioRxiv - Microbiology 2020Quote: ... The cells were homogenized using an Avestin EmulsiFlex homogenizer and lysed using sonication (programme 7, Fisher Scientific™ Model 705 Sonic Dismembrator) and the resultant lysate was transferred into SS34 tubes for further centrifugation at 18,000 rpm (JA-25.50 rotor ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems QuantStudio Flex 7 Real Time PCR System (Thermo Fisher Scientific). The ΔΔCT method was used for relative quantification of target mRNA expression and normalized to Rn18s (18S rRNA ...
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bioRxiv - Developmental Biology 2020Quote: ... samples from pools of 20 medaka hatchlings (stage 40) and human fibroblasts (7×105 cells) were lysed with RIPA Lysis and Extraction Buffer (Thermo Scientific, #89900). Samples were purified with methanol/chloroform protein extraction protocol according to (Wessel & Flügge ...
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bioRxiv - Developmental Biology 2020Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems Quant Studio Flex 7 Real Time PCR System (Thermo Fisher Scientific). The ΔΔCT method was used for relative quantification of target mRNA expression level normalized to Rn18s (18S rRNA) ...
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bioRxiv - Developmental Biology 2020Quote: ... QPCR on technical triplicates for each sample was performed on 5 ng of relative RNA using Takyon Low ROX SYBR 2X MasterMix blue dTTP (Takyon) on a ViiA™7 thermal cycler (Applied Biosystems). Ct values of all genes analysed were normalized to the geometrical mean of Ct values of three housekeeping genes (actinb2 ...
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bioRxiv - Neuroscience 2022Quote: Bacterial load was determined as described previously by Nadkarni et al.(Nadkarni et al., 2002) using Taqman and ViiA™7 Real-Time PCR System (Life Technologies). PCR consisted of 40 cycles ...
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bioRxiv - Molecular Biology 2020Quote: ... PR2 or GFP PCR amplicons of about 500 pb (Supplementary Table 7) were used as templates to produce random labelled probes using the Decalabel DNA Labelling kit (Thermo Fisher Scientific) and [α32P]-dCTP according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... we performed a Gibson Assembly to insert the Kate2 expressing oligo ordered as a gene string from Invitrogen (Supplementary Data Table 7).
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bioRxiv - Developmental Biology 2019Quote: ... Gene expression analysis was performed using PerfeCTa SYBR Green FastMix (Quanta, Cat No. 95073) on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific). The data presented correspond to the mean 2-ΔΔCt after being normalized to the geometric mean of β-actin ...
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bioRxiv - Bioengineering 2019Quote: ... Culture medium for MDA-MB-231 (HTB-26™, ATCC, US), and MCF-7 (HTB-22D™, ATCC, US) culturing in DMEM medium (Gibco−) supplemented with 10% PBS and 1% 100x antibiotic-antimycotic (Anti-Anti ...
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bioRxiv - Neuroscience 2019Quote: ... while nine tenth of the samples were analyzed via Coomassie staining after separation on 7% NuPAGE™ Tris-Acetate protein gels (Thermo Fisher) in NuPAGE™ Tris-Acetate SDS running buffer (Thermo Fisher) ...
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bioRxiv - Microbiology 2019Quote: ... Each sample was quantified by targeting tp0574 through qPCR using a 96-well reaction plate with a ViiA 7 Real-Time PCR System (Applied Biosystems, USA) according to the protocals already established in the laboratory [31] ...
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bioRxiv - Biochemistry 2019Quote: The N30 Random DNA pool and cDNA from rounds 7-to-9 were cloned into TOPO™ TA Cloning™ Kit (Invitrogen) following manufacturer’s specifications ...
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bioRxiv - Immunology 2019Quote: ... MDSCs and monocytes were incubated at a concentration of 1-7×105 cells/well in FluoroBrite Dulbecco’s Modified Eagle Medium (DMEM, ThermoFisher Scientific, Waltham, MA) supplemented with 10% human serum ...
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bioRxiv - Neuroscience 2019Quote: Culture medium from 7 div OBSCs was assayed for human Aβ1-40 and Aβ1-42 using commercially available ELISA kits (Life Technologies: KHB3441 and KHB3481). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... The retina was then transferred to a microcentrifuge tube and incubated for 7 minutes at 37°C with an activated papain dissociation solution (87.5 mM HEPES pH 7.0 (Thermo Fisher Scientific, cat. #15630080), 2.5 mM L-Cysteine (MilliporeSigma ...
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bioRxiv - Genetics 2020Quote: ... Blocks were later cut on a cryostat and 7-10 µm thick sections were placed on SuperFrost Plus Gold slides (ThermoFisher; Cat# FT4981GLPLUS). Hematoxylin and eosin (H&E ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded onto 384-well plates in triplicate and run on a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific) where gene expression was normalized to β-actin and determined using the ΔΔCt formula.
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bioRxiv - Cancer Biology 2019Quote: Ccr2+/+ or Ccr2−/− cancer cells were isolated from tumors and sorted as EpCAM+CD45-CD31- live cells (using 7-AAD Viability Staining Solution, Thermo Fisher Scientific) four weeks after orthotopic transplantation into the mammary glands of wild-type C57BL/6 mice ...
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bioRxiv - Cell Biology 2020Quote: ... The mEGFP-tagged Tom20 line was transfected with mCherry-Mito-7 construct (Michael Davidson, addgene #55102) using 6 μl per well of transfection mixture containing 25 μl Opti-MEM (ThermoFisher #31985-070), 1.5 μl GeneJuice (Millipore #70967 ...
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bioRxiv - Genomics 2020Quote: ... PMPs were harvested from suspension during medium exchange and plated in microglia differentiation media over 7-14 days to produce microglia like cell monocultures (Neurobasal (Life Technologies 21103049) supplemented with Gem21 NeuroPlex without Vitamin A (GeminiBio ...
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Neuroscience 2021Quote: ... and the pellet was gently resuspended in 800 μl of wash buffer before 7-aminoactinomycin D (Thermo Fisher Scientific, 00-6993-50) staining and fluorescence-activated cell sorting (FACS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells (1 × 107) from passage 5-7 were used for transplantation after staining with carboxyfluorescein succinimidyl ester (CSFE) (Invitrogen, Carlsbad, California, USA) on the day of the transplantation ...
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bioRxiv - Cell Biology 2021Quote: ... we performed a TaqMan quantitative real-time PCR approach to quantify sjTREC expression (signal joint excision circles produced during T-cell development) using ViiA™ 7 Real-Time PCR System (Life technologies). TaqMan qPCR was performed on 75-100 ng DNA in a 12 μl reaction mixture containing 18 µM of each primer for sjTREC (5’-TCGTGAGAACGGTGAATGAAG-3’ and 5’-CCATGCTGACACCTCTGGTT-3’ ...
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bioRxiv - Biochemistry 2021Quote: 10 uL of either Caspase-3 or −7 library glycerol stocks was used to inoculate 5 mL of auto-induction media (Invitrogen Magic Media) and allowed to incubate and express for 18 hours at 30 °C ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were sectioned to 7 – 15 μm thickness and deparaffinized before staining with hematoxylin (Catalog No. SDGHS280, Thermo Fisher Scientific, Waltham, MA) and eosin (Catalog No ...
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bioRxiv - Cell Biology 2021Quote: mtDNA and nDNA were quantified by Taqman qPCR on the ViiA 7 real-time PCR system (Applied Biosystems, Foster City, CA, USA) using equine- and bovine-specific primers and probes ...
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bioRxiv - Microbiology 2020Quote: ... Roots were removed from the tube and the quantity of bacterial cells detached from the roots and recovered in the NaCl solution was evaluated using Real Time PCR (qPCR) by a standard curve method on the nodB gene in a QuantStudio™ 7 flex (Applied Biosystems), as previously described [47 ...
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bioRxiv - Neuroscience 2020Quote: We dissected brains and ventral nerve cords of 5-7 days old males in Schneider’s insect medium (Thermo Fisher Scientific, Waltham, MA) and fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were transferred on PVDF membranes (Roth) for 7 min at 20 V using the iBlot dry blotting system (Thermo Fisher Scientific), before blocking in TBS-T with milk.
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bioRxiv - Neuroscience 2020Quote: ... mice were first anesthetized with a ketamine (100 mg/kg) and xylazine (7 mg/kg) mixture then perfused with 4% paraformaldehyde (Thermo Fisher Scientific), and the brains were extracted and cryoprotected in 30% sucrose in 1X PBS until they sank ...
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bioRxiv - Immunology 2021Quote: The enumeration of apoptotic cells was performed using the CellEventTM Caspase-3/7 Green Flow Cytometry Assay Kit (catalogue #: C10427; Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... ATPase activity was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside–purine nucleoside phosphorylase reaction to detect released inorganic phosphate (EnzChek kit; Thermo Fisher Scientific) (62) ...
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bioRxiv - Plant Biology 2020Quote: ... cinerea germlings exposed to different concentrations NCR044.1 was measured every 30 min for 2 h using the ROS indicator dye 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA, Invitrogen, Carlsbad, CA). ROS levels were quantified by measuring the fluorescence in a SpectraMax® M3 spectrophotometer microplate reader (exc ...
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bioRxiv - Microbiology 2019Quote: ... or 250 ng (Fig. 7) of RNA were used to synthesize cDNA with the SuperScript™ II Reverse Transcriptase kit (ThermoFisher Scientific). Real-time PCR was performed using 2µl of cDNA and QuantiTect SYBR green PCR master (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Cells were then stained intracellularly with guinea pig anti-NS6/7 (kind gift from Kim Green) and goat anti-guinea pig Alexa Fluor 647 (1:500, Life Technologies, #A21450). Cells were then washed twice with PWB and then resuspended in FACS buffer.
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bioRxiv - Cancer Biology 2021Quote: ... and PCR primers (200 nM) in a total volume of 25 µl and analyzed on a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Each individual biological sample was amplified in technical duplicate and normalized to GAPDH as an internal control ...
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bioRxiv - Cancer Biology 2021Quote: ... For the analysis of STAT3/5 phosphorylation cells were also labeled with phycoerythrin (PE)-cyanine 7 (Cy7)-conjugated anti-phospho-STAT3 (Tyr705) (Thermo Fisher Scientific) (#25-9033-42 ...
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bioRxiv - Cell Biology 2021Quote: ... Human breast carcinoma cell lines (SK-BR-3, MCF-7, MDA-MB-231, T-47D) were cultured in GIBCO® RPMI 1640 (Life technologies) medium supplemented with 10% FBS (Life technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... Relative gene expression was determined by quantitative real time PCR (q-RT-PCR) using the ViiA 7 Real Time PCR system (Applied Biosystems, USA). The reaction mixture consisted of 0.75μL 500nM forward primer ...
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bioRxiv - Biochemistry 2022Quote: Capillary electrophoresis of FAM-labeled RNA was carried out using an Applied Biosystems 3730xl Genetic Analyzer (96 capillary array) using POP-7 polymer and GeneScan® 120 LIZ dye Size Standard (Applied Biosystems) (Greenough et al ...
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bioRxiv - Molecular Biology 2022Quote: ... HCA-7 and MDCK cells were allowed to differentiate for 7 days after plating at 100% confluency in the presence of 2 µg/mL doxycycline (Fisher Scientific AAJ6042203) when inducing transgene and/or reporter transcript expression ...