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Citations for Thermo Fisher :
2101 - 2150 of 8052 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Barcoding primers were purchased from Eurofins Genomics at 100 μM concentration in HPLC-purified, 96-well plate format. Barcoded amplicons (e.g., Supplementary Fig. 7) were quantified by Qubit fluorometry (Thermo Fisher Scientific), and pooled at equimolar concentrations ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were double-blinded microinjected into the cytoplasm of single COS-7 cells (n=100-200 cells per sample) along with fluorescein-labeled dextran (molecular weight 10000, Life Technologies) using a micromanipulator (Narishige) ...
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bioRxiv - Microbiology 2020Quote: ... primers for different viral and cellular transcripts (see Supplementary Table 7 for complete list of primers) and SYBR Green (Thermo Scientific) using the QuantStudio 7 Flex Real-Time PCR System (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... in a 10 µl reaction volume run in triplicate wells of a 96 well MicroAmp plate run on a QuantStudio 7 qPCR cycler (Thermo Fisher) and QuantStudio Real-Time PCR Software v1.3.
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Immunology 2021Quote: ... immature DCs were generated from bone marrow cells and cultured for 7 days in Iscove’s Modified Dulbecco’s Media (IMDM; Gibco Cat# 12440-053) containing 10% fetal bovine serum Atlanta biologicals Cat# S12450H ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Isolated cells were incubated in sterile petri dishes for 7 to 10 days in BMDM differentiation medium – DMEM (Gibco, 11965-092) supplemented with 30% L-cell conditioned medium ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Immunology 2021Quote: ... BirA enzyme was added at a ratio of 1:200 w/w and incubated for 1 h at 37 °C before being buffer exchanged into PBS [pH 7.4] with a Zeba 7 kDa MWCO spin column (Thermo Fisher Scientific).35
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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NVX-CoV2373 vaccine protects cynomolgus macaque upper and lower airways against SARS-CoV-2 challengebioRxiv - Immunology 2020Quote: ... Reactions were carried out on a Quant Studio 6 and 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts were differentiated on cell culture plastic for 7 days before transfection with two separate siRNAs targeting either MMP8 (105332, 112911; Life Technologies) or MMP9 (104072 ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Microbiology 2020Quote: COS-7 cells were seeded onto 6-well plates 24 hours prior to the transient transfection using Lipofectamine 2000 reagent (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 30 minutes with 40 µl/ml CellEvent™ Caspase-3/7 Green ReadyProbes Reagent (#R37111, Life Technologies). Cells were washed with PBS (without Mg2+ and Ca2+) ...
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bioRxiv - Immunology 2022Quote: ... RNA levels were measured by the SYBR Green relative quantitation method using either a 7300 or Viia 7 RT-PCR machine (Applied Biosystems). Samples were normalized by subtracting the respective CT values of housekeeping genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was retrotranscribed and amplified in an QuantStudio 7 Flex thermocycler (Applied Biosystem) using Taqman RNA-to-Ct 1-Step Kit (Thermofisher, 4392653). The Taqman probes used were the following Jag1 (Mm00496902_m1) ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 10 ng cDNA for each sample was used and all qRT-PCR reactions were performed on a ViaA 7 Real-Time PCR System (Life Technologies) using Brilliant II SYBR Green QPCR Master Mix (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1x PBS twice and incubated for 20 min in dark at 37°C with 20 µM of H2DCFDA (2’,7’- dichlorodihydrofluorescein diacetate; I36007, Thermo Fisher) diluted in serum-free medium ...
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bioRxiv - Genomics 2022Quote: ... Duplicates of 10 μL of each sample were transferred into a qPCR plate and measured in a Viia 7 qPCR machine (Applied Biosystems) using following program ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...
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bioRxiv - Genomics 2022Quote: ... Slides were then placed in a hybridization chamber, and 75μl of preheated FISH solution (0.9M NaCl, 20mM Tris pH 7 (both Thermo Fisher Scientific, USA), 0.1% SDS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 7 Flex). Primers used for qPCR reactions are listed in Supplementary Table S5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Neuroscience 2022Quote: To analyze effector caspase activity in stimulated hippocampal neurons we employed the CellEvent(tm) caspase-3/7 green detection assay (ThermoFisher Scientific) that allows analyzing activity in individual cells ...
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bioRxiv - Microbiology 2022Quote: ... with 250ng each of the 8 plasmids corresponding with each segment of viral genome (or 7 plasmids for mock) and 4 µl Lipofectamine 2000 transfection reagent (Thermo Fisher) per well ...
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bioRxiv - Immunology 2022Quote: 22-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-23,24-Bisnor-5-Cholen-3β-Ol-cholesterol (22-NBD-cholesterol) (N1148) and 7-NBD-PE (N360) were obtained from Thermo Fisher and dissolved in ethanol at 1 mM ...
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bioRxiv - Neuroscience 2022Quote: ... and medium from day 3-7 is Neurobasal Plus medium (NB-Plus) supplemented with B-27 μg/mL (Thermo Fisher Scientific), GlutaMax 10 μg/mL (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Single-axis tilts were collected from −60° to +60° at 2° increments at 7 □µm defocus on a Falcon 3 camera (Thermo Fisher) operated in linear mode at 22,000X magnification ...
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bioRxiv - Microbiology 2023Quote: ... Proteins RNAylated with Cy5-labelled RNA were equilibrated in the same buffers using Zeba Spin desalting columns (7 MWCO, 0.5 ml) (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2022Quote: Total RNA from the whole bodies (four insects in each biological replicate/sample) and from MT (7-10 MTs in each sample/biological replicate) was isolated with TRIzol (Invitrogen, 15596026). Isolated RNA samples were treated with DNase I (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: Gene expression of targets listed in supplemental table 2 were analysed by SYBR green-based qPCR analysis using Applied Biosystems QuantStudio 7 Real-Time PCR Systems (Thermo Scientific), following the standard PCR cycling sequence using GAPDH as an internal control ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative RT-PCR was performed using SYBR green Advanced Master Mix and run on a ViiA 7 Real-Time PCR System (all from Applied Biosystems). Primers were in-house designed (see table 1 ...
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bioRxiv - Microbiology 2023Quote: ... plated at a density of 7 x 106 cells in a 175 cm2 tissue culture flask in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Pathology 2023Quote: ... The mRNAs were quantified using individual TaqMan assays described in Supplemental Table 2 on an ABI QuantStudio 7 Real-Time PCR machine (Applied Biosystems) using TaqMan Fast Advanced Master Mix (Thermo Fisher ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR program comprised an initial denaturation for 30 s at 95 °C and amplification by 40 cycles of 15 s at 95 °C and 1 min at 60 °C and was ran in an ABI QuantStudio 7 Real Time PCR machine (Applied Biosystems). Expression values of DIN6 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was reverse transcribed with qScript cDNA supermix (Quanta Bio, #95048-500) and qRT-PCR was performed with QuantaStudio 7 Real-time qPCR System (Thermofisher, #A43183). Transcript levels of Htr2c ...
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Early life lipid overload in Native American myopathy is phenocopied by stac3 knock out in zebrafishbioRxiv - Genetics 2023Quote: ... Gene expression was quantified using the PowerUp SYBR Green Master Mix and a ViiA™7 Dx qPCR Instrument (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... and 0.1 mM AMPPNP) using a 0.5-mL Zeba spin desalting column (7-kD molecular weight cutoff, Thermo Fisher Scientific, #89882). AMP-PNP and Taxol were added to the flow-through to final concentrations of 1 mM and 10 µM ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative real-time PCR reactions (TaqMan and SYBR Green) were performed on a ViiA 7 Real-Time PCR System (Applied Biosystems). For quantitative real-time PCR of selected TFs ...
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bioRxiv - Neuroscience 2023Quote: ... at 1,000g for 2 min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Molecular Biology 2023Quote: SGBS cells in the undifferentiated state or on day 7 of differentiation were electroporated using the Neon™ Transfection System (Invitrogen) with C-terminally tagged target genes or GFP control ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA was purified from the same cultures after 7 hours of growth using GeneJET RNA Purification Kit (Thermo Fisher Scientific) and analyzed in the same way ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Zoology 2023Quote: ... kit and sequenced via ABI 3730XL DNA sequencer platform using the BigDye Terminator (version 3.1) Cycle Sequencing Kit and POP-7 Polymer separation matrix (Applied Biosystems, Inc.) using both forward and reverse primer ...
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bioRxiv - Cancer Biology 2023Quote: ... dense cultures containing organoids ranging in size from 100 to 500 µm were passaged every 7-10 days with TrypLE (Life Technologies) containing 10 mM ROCK inhibitor and 10 mg/mL DNase I (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2023Quote: ... and qPCR for Sfrp2 was performed using SYBR Green qPCR Reagent (Quantabio) on a ViiA 7 Real-Time PCR machine (Applied Biosystems). Primers used for qPCR are listed in Extended Data Table 2.