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Citations for Thermo Fisher :
2451 - 2500 of 8618 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Cultures were washed three times with washing solution and then incubated for 30 min at room temperature on the OrganoFlow (7°, 2-min intervals) with Alexa Fluor 488 (#a32731; Invitrogen; 1:250) and Alexa Fluor 647 (#a31571 ...
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bioRxiv - Genomics 2022Quote: ... was carried out on a StepOnePlus 96-well or a QuantStudio 7 Flex 384-well Real-Time PCR System (Thermo Fisher Scientific) loading 20-25ng cDNA /well and using TaqMan Fast Advanced Master-Mix (Thermo Fisher Scirentific ...
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bioRxiv - Immunology 2022Quote: Real-time RT-PCR was performed using the ViiATM 7 Real-Time PCR System and Power SYBRTM Green PCR Master Mix (ThermoFisher Scientific #4368708). Primers for HA (forward 5’-AAACTCTTCGCGGTCTTTCCA-3’ ...
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bioRxiv - Microbiology 2022Quote: Sucrose gradient purified fractions of yeast-derived PV-3 SC8 were pooled and buffer exchanged into PBS + 20mM EDTA (pH 7) using Zeba Spin Desalting Columns with a 7K molecular weight cut-off (MWCO) (Thermo Fisher Scientific) and concentrated using Amicon Ultra centrifugal filter devices (100 kDa MWCO ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed three times with unsupplemented neurobasal media and loaded with 200μM 2’,7’-dichlorofluorescein diacetate (DCFH-DA) fluorescent dye (Thermo Fisher Scientific, MA) for 30 minutes at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were separated by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in either homemade 7% or 4-12% gradient Bis-Tris polyacrylamide gel (Thermo Fisher Scientific) using 3-(N-morpholino)propanesulfonic acid (MOPS ...
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bioRxiv - Plant Biology 2022Quote: ... Mock-treated and infected roots of WT and HvMPK3 KO plants were incubated in 30 μM 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA; Cat no. C6827, Invitrogen™, USA) and PI (1 mg.ml-1 ...
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bioRxiv - Microbiology 2022Quote: ... at 750 nm of 0.3 and monitored for 7 days by measuring OD at 750 nm every 24 hours using a GENESYS 10S UV-Vis Spectrophotometer (Thermo Scientific, Germany). For PCC 6803 ...
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bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR of diluted ChIP DNA and corresponding input DNA was performed on ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Primer sequences used for ChIP are listed in Supplementary Table 1 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated for 7 min at 37°C with proteinase K in PBST (1/2000 from a glycerol stock at 20mg/ml, Ambion, Cat#10259184), washed in PBST for 2×10 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Immunology 2024Quote: ... then collected and plated 10:1 with allogeneic DC-10s in 24-well plates at a concentration of 7 × 105 to 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco, Thermo Fisher Scientific). For CRISPRa experiments ...
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bioRxiv - Neuroscience 2024Quote: ... were further evaluated for the quantitative real-time PCR (qRT-PCR) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative quantification of the long TMEM106B 3’ UTR and total TMEM106B levels was determined using the ΔΔCt method and normalized to two endogenous controls ...
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bioRxiv - Bioengineering 2024Quote: Cell count was measured on days 1 and 7 using a using Quant-iT™PicoGreen™dsDNA Assay Kit (ThermoFisher Scientific). The scaffolds were washed for 5 minutes in PBS and then frozen at -80⍰C ...
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bioRxiv - Developmental Biology 2024Quote: Ovaries from 5-7 females were dissected in 1% PBS-Tween and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) and heptane (Sigma Aldrich ...
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bioRxiv - Genomics 2023Quote: ... Cells were then collected by centrifugation at 1200 RPM for 7 min and washed twice using ice-cold PBS (Gibco Cat# 10010002). Collected pellets were lysed using Lysis/Binding Buffer (Invitrogen Cat# 61012) ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membrane at 20 V for 7 minutes using the iBlot™ 2 Transfer Stack (Thermo Fisher Scientific). Membranes were blocked in 5% non-fat milk powder in TBS+0.1% Tween (TBST ...
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bioRxiv - Cell Biology 2022Quote: Fast-growing human embryonic kidney 293T cells (HEK293FT) were maintained in DMEM 10% FBS (v/v) and passaged at 5-7 day intervals with TrypleExpress (Invitrogen GIBCO, Paisley, UK,) to detach the cells ...
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bioRxiv - Microbiology 2022Quote: ... washed twice with 20 mM MOPS buffer (pH 7) and concentrated to reach a final A578 of 10 in a 96 well-plate (ref. 4483481, Applied Biosystems™) containing cytochrome c (0.25 mg/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... was added to the media at 100 μ for 7 minutes before lysis and the cell lysate was cleared and digested with Turbo DNase (Invitrogen, AM2238). The cleared lysate was subjected to ultracentrifugation at 28000 rpm for 3 hours at 4 °C and separated on a 5% -50% sucrose gradient.
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bioRxiv - Cancer Biology 2023Quote: ... Eluted phosphopeptide samples from both enrichments were pooled and fractionated into 14 fractions by increasing ACN concentration from 7 % to 50 % using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC ...
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bioRxiv - Biophysics 2023Quote: ... samples were stained for 90 min with 1.65 x 10−7 M Alexa Fluor 488 phalloidin (Invitrogen, Life Technologies Corporation, Oregon, USA) in 1x DPBS ...
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bioRxiv - Neuroscience 2023Quote: Larval zebrafish at 4–7 days post-fertilization were anesthetized (0.025% MS-222, Syndel) and mounted in 2% low melting point agarose (Thermo Fisher Scientific 16520), dorsal-up ...
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bioRxiv - Biophysics 2023Quote: The NP-RNA assembly experiment (Fig. 7) was performed on a Q Exactive Ultra-High Mass Range (UHMR) Orbitrap mass spectrometer (Thermo Fisher Scientific) in positive ion mode with a mass range from 350 to 20.000 m/z and a nano ESI source ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were cut with a microtome in the transverse plane at a thickness of 7-10 µm and mounted onto Superfrost Plus slides (Thermo Fisher Scientific). Mounted sections were dried at room temperature for a minimum of 1 week before immunohistochemistry ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were transferred for 7 min at 22-25 V onto a PVDF membrane using the iBlot2 transfer system (Thermo Fisher Scientific). Membranes were blocked with 5% BSA TBST for 1h at 24 prior to incubating with STAT3 (Cat #12640 ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells were plated with antibiotic-free medium at a density of 7 × 106 cells in a T75 flask and transfected via Lipofectamine3000 (Invitrogen, Carlsbad, CA) using a total of 15 µg of BEI lentiviral plasmids (Table 1) ...
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bioRxiv - Bioengineering 2023Quote: ... Cryopreserved hiPSC-derived endothelial cells were thawed in a water bath at 37 °C and transferred into a 15 mL tube containing 7 mL of Human Endothelial SFM medium (11111044; Thermo Fisher Scientific). The cells were centrifuged at 300 x g for 3 min and the pellet was resuspended in Human Endothelial SFM complete medium ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Cancer Biology 2023Quote: ... then plated in duplicate in 7 μL medium at 125 cells per well into compound-containing wells using a Multidrop Combi Reagent Dispenser (Thermo Scientific 5840320) plate dispenser ...
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bioRxiv - Cancer Biology 2022Quote: ... Approximately 3,000 MCF-7 cells and 10,000 HCC1500 cells were seeded in black 96-well optical bottom plates (Thermo Scientific™, CAT: 165305) in 100 µL per well ...
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bioRxiv - Immunology 2023Quote: ... diluted to 2 μM in warm RPMI-1640 and incubated at 37°C for 30 min before washed and stained with 7-AAD (Invitrogen, cat # A1310).
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bioRxiv - Immunology 2023Quote: ... The cells were then stained with antibody cocktail (Supplementary Table 7) along with LIVE/DEAD Fixable Aqua Dead Cell Stain (Invitrogen; cat # L34957) or eBioscience Fixable eFluor780 viability dye (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Cos-7 cells (ATCC) were cultured in DMEM supplemented with 10% FBS and 100 U/ml penicillin/streptomycin (all Thermo Fisher Scientific) in 10 cm culture dishes ...
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bioRxiv - Bioengineering 2022Quote: The level of reactive oxygen species was assessed in the homogenate supernatant of RPE and retina tissue with using 2′–7′-dichlorofluorescein diacetate (DCFH-DA; ThermoFisher Scientific Inc.) molecular probes ...
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bioRxiv - Neuroscience 2023Quote: ... according to the manufacturers protocol and was read on an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantstudioTM Software by Applied Biosystems. The layout of the PCR array 96-well plate consisted of probes/primers for 84 inflammasome-associated genes ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed using an Applied Biosystems® ViiA 7 Real-Time PCR System and analysis was conducted on QuantStudioTM Software by Applied Biosystems. Minus reverse transcriptase and no-template cDNA controls were included ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... The starvation assay consisted of placing 5-7 day-old female DTKR-RNAi flies and control flies into vials containing 1% aqueous agar (Fisher Scientific, USA) [48,73].
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bioRxiv - Molecular Biology 2023Quote: ... The iPSCs were then cultured for 7 days in Neurobasal/DMEM-F12 medium (1:1 v/v) containing 2% B27 (Gibco, 17054–044), 1% N2 (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixtures were heated from 10°C to 70°C for approximately 1 hr by a QuantStudio 7 Pro Real-Time PCR System (Thermo Fisher Scientific). Approximately 770 evenly spaced measurements of fluorescence against the temperature gradient were recorded and processed by QuantStudio V1.3 (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was extracted from 40 pairs of adult testes per sample per condition (3 to 7 days post-eclosion) using the PureLink RNA Mini Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 5mM MgSO4 pH 7 with KOH) and root tip dissection on polylysine-coated Epredia® SuperFrost Ultra Plus Gold slides (ThermoFisher Scientific), the slides with the root samples were mounted under a counter slide using 1X MTSB solution and positioned in the InsituPro VSi holder ...
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bioRxiv - Immunology 2023Quote: ... and real-time PCR was performed on a LifeTechnologies QuantStudio 7 real-time PCR system using SYBR™ Green PCR Master Mix (Applied Biosystems). Gene expression levels were normalized to Actb or Gapdh ...
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bioRxiv - Cancer Biology 2023Quote: siRNA screening was performed in MCF-7 TEAD reporter cells using two different siRNA libraries (Dharmacon On-Target Plus and Ambion Silencer Select) targeting ubiquitin-related genes ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time reverse transcription polymerase chain reactions (RT-PCRs) were performed on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems, Life Technologies). Data were normalized to housekeeping genes (Tubulin ...
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bioRxiv - Cell Biology 2023Quote: ... HepG2 and Huh-7 (procured from Cell Repository, NCCS, Pune, India) were cultured in Dulbecco’s Modified Eagle Medium (Gibco, USA; Hyclone, USA) supplemented with 0.5% pen/strep solution and 10% fetal bovine serum (Gibco ...