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Citations for Thermo Fisher :
2201 - 2250 of 8618 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Differential scanning fluorimetry protein thermal shift assays were carried out using a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Reactions were pipetted into wells in a MicroAmp FAST optical 96-well plate (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... at a concentration of 7 μM in the electroporation slurry (as previously described in Alex et al., 2019) (Neon Transfection System, Thermo Fisher). Control cells were electroporated with mCherry or electroporation buffer ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). The following thermal cycling conditions were used ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction solution was then purified via Zeba spin desalting columns with 7 kDa cutoff to remove unreacted linker (Thermo Fisher). Successful linker conjugation was confirmed using gel electrophoresis and comparison to a size standard of the unmodified Spike ...
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bioRxiv - Bioengineering 2021Quote: ... The reaction solution was then purified via Zeba spin desalting columns with 7 kDa cutoff to remove unreacted linker (Thermo Fisher). Successful linker conjugation was confirmed using gel electrophoresis and comparison to a size standard of the unmodified RBD ...
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bioRxiv - Neuroscience 2021Quote: ... Percent of cell death was determined by staining with 7 μM Hoechst 33342 and 2 μM propidium iodide (PI) (Invitrogen, USA). PI were diluted in warm neuronal culture medium and incubated for 5 min and then images were taken by a Zeiss microscope equipped with automated computer assisted software (Axiovision 4.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR reactions were performed in technical and biological triplicates in a ViiA 7 Real-Time PCR system (Thermo Fisher Scientific). Microsoft Excel (v16.54 ...
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bioRxiv - Cell Biology 2021Quote: ... day 7 and day 19 samples all cells were adherent and collected using 2 ml/well accutase (Thermo Fisher Scientific, A1110501) and a cell scraper ...
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bioRxiv - Biophysics 2021Quote: ... 25 mM NaCl and 10 mM β-Mercaptoethanol at pH 7 (pH adjusted with NaOH) was used and 880 units per ml SEC sample RNAseOUT(Invitrogen) as RNAse inhibitor were added to the sample prior to the size exclusion chromatography purification step ...
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bioRxiv - Genetics 2020Quote: ... Barcoding primers were purchased from Eurofins Genomics at 100 μM concentration in HPLC-purified, 96-well plate format. Barcoded amplicons (e.g., Supplementary Fig. 7) were quantified by Qubit fluorometry (Thermo Fisher Scientific), and pooled at equimolar concentrations ...
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bioRxiv - Neuroscience 2020Quote: ... The samples were double-blinded microinjected into the cytoplasm of single COS-7 cells (n=100-200 cells per sample) along with fluorescein-labeled dextran (molecular weight 10000, Life Technologies) using a micromanipulator (Narishige) ...
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bioRxiv - Microbiology 2020Quote: ... primers for different viral and cellular transcripts (see Supplementary Table 7 for complete list of primers) and SYBR Green (Thermo Scientific) using the QuantStudio 7 Flex Real-Time PCR System (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... in a 10 µl reaction volume run in triplicate wells of a 96 well MicroAmp plate run on a QuantStudio 7 qPCR cycler (Thermo Fisher) and QuantStudio Real-Time PCR Software v1.3.
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Immunology 2021Quote: ... immature DCs were generated from bone marrow cells and cultured for 7 days in Iscove’s Modified Dulbecco’s Media (IMDM; Gibco Cat# 12440-053) containing 10% fetal bovine serum Atlanta biologicals Cat# S12450H ...
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bioRxiv - Immunology 2021Quote: Thermal melt analysis of the recombinant proteins was performed in triplicate in 96-well plates in a QuantStudio 7 Flex real time PCR machine (Applied Biosystems). Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Isolated cells were incubated in sterile petri dishes for 7 to 10 days in BMDM differentiation medium – DMEM (Gibco, 11965-092) supplemented with 30% L-cell conditioned medium ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Immunology 2021Quote: ... BirA enzyme was added at a ratio of 1:200 w/w and incubated for 1 h at 37 °C before being buffer exchanged into PBS [pH 7.4] with a Zeba 7 kDa MWCO spin column (Thermo Fisher Scientific).35
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... FW 5’-CTCTCTGGGCGAAATCTGCT-3’ and REV 5’-GAGTGCTTTCGCTATGTTGTTCA-3’ for Clmn and FW 5’-TGATGGGTGTGAACCACGAG-3’ and REV 5’-GCCGTATTCATTGTCATACCAGG-3’ for Gapdh using a QuantStudio 7 (Applied Biosystems). Transcript levels are expressed as the ratio of 2−ΔCT (Transcript of interest)/2−ΔCT (Gapdh).
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NVX-CoV2373 vaccine protects cynomolgus macaque upper and lower airways against SARS-CoV-2 challengebioRxiv - Immunology 2020Quote: ... Reactions were carried out on a Quant Studio 6 and 7 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts were differentiated on cell culture plastic for 7 days before transfection with two separate siRNAs targeting either MMP8 (105332, 112911; Life Technologies) or MMP9 (104072 ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Microbiology 2020Quote: COS-7 cells were seeded onto 6-well plates 24 hours prior to the transient transfection using Lipofectamine 2000 reagent (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 30 minutes with 40 µl/ml CellEvent™ Caspase-3/7 Green ReadyProbes Reagent (#R37111, Life Technologies). Cells were washed with PBS (without Mg2+ and Ca2+) ...
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bioRxiv - Immunology 2022Quote: ... RNA levels were measured by the SYBR Green relative quantitation method using either a 7300 or Viia 7 RT-PCR machine (Applied Biosystems). Samples were normalized by subtracting the respective CT values of housekeeping genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was retrotranscribed and amplified in an QuantStudio 7 Flex thermocycler (Applied Biosystem) using Taqman RNA-to-Ct 1-Step Kit (Thermofisher, 4392653). The Taqman probes used were the following Jag1 (Mm00496902_m1) ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 10 ng cDNA for each sample was used and all qRT-PCR reactions were performed on a ViaA 7 Real-Time PCR System (Life Technologies) using Brilliant II SYBR Green QPCR Master Mix (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 1x PBS twice and incubated for 20 min in dark at 37°C with 20 µM of H2DCFDA (2’,7’- dichlorodihydrofluorescein diacetate; I36007, Thermo Fisher) diluted in serum-free medium ...
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bioRxiv - Genomics 2022Quote: ... Duplicates of 10 μL of each sample were transferred into a qPCR plate and measured in a Viia 7 qPCR machine (Applied Biosystems) using following program ...
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bioRxiv - Immunology 2022Quote: ... reactions were heated to 70 °C for 2 min and 15 μl loaded on 15% polyacrylamide gels containing 7 M urea (Invitrogen, EC8852BOX). Low range ssRNA Ladder (NEB ...
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bioRxiv - Genomics 2022Quote: ... Slides were then placed in a hybridization chamber, and 75μl of preheated FISH solution (0.9M NaCl, 20mM Tris pH 7 (both Thermo Fisher Scientific, USA), 0.1% SDS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 7 Flex). Primers used for qPCR reactions are listed in Supplementary Table S5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Cancer Biology 2024Quote: ... Biozym Scientific GmbH) was used and analysis was done on a ViiA 7 Real-Time PCR System (4453536, Thermo Fisher Scientific). mRNA levels were normalized to the geometric mean of Cyclophilin A and hypoxanthine guanine phosphoribosyl transferase and/or 18S ...
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Neuroscience 2022Quote: To analyze effector caspase activity in stimulated hippocampal neurons we employed the CellEvent(tm) caspase-3/7 green detection assay (ThermoFisher Scientific) that allows analyzing activity in individual cells ...
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bioRxiv - Microbiology 2022Quote: ... with 250ng each of the 8 plasmids corresponding with each segment of viral genome (or 7 plasmids for mock) and 4 µl Lipofectamine 2000 transfection reagent (Thermo Fisher) per well ...
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bioRxiv - Immunology 2022Quote: 22-(N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)Amino)-23,24-Bisnor-5-Cholen-3β-Ol-cholesterol (22-NBD-cholesterol) (N1148) and 7-NBD-PE (N360) were obtained from Thermo Fisher and dissolved in ethanol at 1 mM ...
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bioRxiv - Neuroscience 2022Quote: ... and medium from day 3-7 is Neurobasal Plus medium (NB-Plus) supplemented with B-27 μg/mL (Thermo Fisher Scientific), GlutaMax 10 μg/mL (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... 7 μl per injection was loaded onto a 2 mm by 0.3 mm Acclaim PepMap C18 trap column (Thermo Fisher Scientific) in 0.1% TFA at 15 μl/min before the trap being switched to elute at 0.25 μl/min through a 50 cm by 75 μm EASY-Spray C18 column ...
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bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative PCR (qPCR) was performed using standard SYBR green reagents and protocols on a QuantStudio 7 Real-Time PCR system (Applied Biosystems). All reactions were performed in technical triplicates ...
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bioRxiv - Cell Biology 2024Quote: COS-7 cells were grown at 37°C with 5% CO2 in complete Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher Scientific, USA) containing 10% FBS (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 2 µL of a 1:10 dilution of 1st strand cDNA was assayed by qPCR on Applied Biosystems Viia-7 instruments in 10 µL reactions with PowerUp SYBR Green master mix (Applied Biosystems) according to manufacturer recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein concentration of the CSF or plasma SEC fractions 7-10 was then determined using Qubit Protein Assay kit (ThermoFisher Scientific) using 1 μL of sample ...