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Citations for Thermo Fisher :
2201 - 2250 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Complementary DNA (cDNA) was generated by reverse transcription polymerase chain reaction (RT-PCR) with SuperScript III (Thermo Fisher). Reverse transcription was performed at 58 °C for 50 min ...
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bioRxiv - Immunology 2024Quote: ... cDNA was synthesized with the SuperScript™ III First-Strand Synthesis System for RT-PCR (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR (RT-qPCR) reactions were performed with Maxima SYBR Green/ROX (K0221; Thermo Fisher Scientific) in Bio-Rad Laboratories CFX96 ...
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bioRxiv - Physiology 2023Quote: Quantitative RT-PCR analyses of candidate genes were performed using SYBR green assays (Life technologies, Saint Aubin, France). We used the housekeeping gene HPRT to normalize relative quantification of mRNA levels ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed using SYBR Power Green PCR master mix with a Viia7 qPCR machine (Thermo Fisher). PCR conditions were set to the following ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time quantitative PCR (RT-qPCR) was performed using a 7500 Fast Real-Time system machine (Applied Biosystems). Gene expression was normalized to the expression of the reference gene Actin beta (ACTB).
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bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed in 384-well plates on a 7900HT Fast Real-Time PCR system (Applied Biosystems) using PowerUp SYBR Green master mix (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... and analyzed by RT-qPCR using Quant Studio 12K Flex Real Time PCR system version 1.1.2 (Applied Biosystems by Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was performed on a Step One Plus Real-Time PCR System (Thermo Fisher Scientific, Catalog #43765592R) using QuantiTect SYBR Green PCR Kit (QIAGEN ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was generated using the Superscript III First-Strand Synthesis System for RT-PCR Kit (Invitrogen, Waltham, MA) per manufacturer’s instructions and all qRT-PCR was performed on a CFX Connect Real-Time System (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... 22RV1 cells were transfected with 20nM miRVana mimic (miR-194 or negative control; Ambion). For all other experiments ...
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bioRxiv - Cancer Biology 2021Quote: MirVana™ miR-212-3p Mimic and scramble negative control were purchased from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 10pg of mirVana miRNA mimic miR-199a-5p (ThermoFisher, Assay ID MC10893 and 002304) was added to 0.1-0.5ug of total RNA as a spike-in control for RT-qPCR normalization ...
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bioRxiv - Cancer Biology 2022Quote: ... and Anti-miR™ miRNA Inhibitor Negative Control #1 (both by Thermo Fisher Scientific) were employed as scrambled controls ...
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bioRxiv - Neuroscience 2021Quote: ... and DRG neurons were then electroporated with miR-544-3p mimic (50 nM; Thermofisher), or miR-544-3p inhibitor (50 nM ...
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Unannotated small RNA clusters in circulating extracellular vesicles detect early stage liver cancerbioRxiv - Cancer Biology 2020Quote: ... and purchased a catalog TaqMan® miRNA Assay against cel-miR-39-3p (ThermoFisher) to target the spike-in miRNA mimic which was used during the exRNA extraction ...
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bioRxiv - Microbiology 2019Quote: Specific miRs were reverse-transcribed using the Taqman MicroRNA reverse transcription kit (Applied Biosystems), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... or miR-K6-5p mimic (miRVana) with Lipofectamine RNAiMax (ThermoFisher Scientific, Cat No: 13778150) as instructed ...
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bioRxiv - Cancer Biology 2019Quote: ... The miR-26a mimic or negative control miRNA (miRNA mimic Negative Control #1, Ambion) was used at a final concentration of 10 nM to investigate the effect of miR-26a on EV secretion ...
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bioRxiv - Cell Biology 2021Quote: ... Pre-miR-181a-1 in vitro synthesis was performed using T7 MEGAshortscriptTM kit (Ambion) from 1 µg purified DNA and following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of miRVANA miR-184 inhibitor (cat. 4464084, ID. MH10207-Invitrogen, Pittsburgh, US) + 50 nM of CRTC1 siRNA ...
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bioRxiv - Cancer Biology 2020Quote: mRNA and miR-7704 levels were measured using the TaqMan Fast Advanced Mix (ThermoFisher) and the following TaqMan Assays with FAM/MGB-NFQ primers/probe ...
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bioRxiv - Developmental Biology 2022Quote: ... We used the mir Vana miRNA Isolation Kit with phenol (Ambion, Austin, TX, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: miRNA mimics of miR-28 and the scramble control sequence were purchased from Ambion. Established DFBL-18689-V2 PDX tumors (> 100 mm3 ...
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bioRxiv - Cell Biology 2023Quote: ... and small RNAs were enriched by an mir-Vana miRNA isolation kit (Thermo Scientific). Samples were pretreated with homemade PIR-1 or 5’ Pyrophosphohydrolase (RppH ...
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bioRxiv - Microbiology 2020Quote: ... the RT reaction was performed using M-MLV RT (Invitrogen) and a random primer set (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... RT was performed with RevertAid RT Reverse Transcription Kit (ThermoFisher). Quantitative PCR was performed with Maxima SYBR Green/ROX qPCR MasterMix (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA quality and quantity were measured using NanoDrop™1000 Quantitative RT-PCR was performed using the ViiA7 Real-Time PCR system (Applied Biosystems, Foster City, CA, USA). Each reaction contained 1X final concentration of SYBR Green PCR Master Mix 2X (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... The oligonucleotide primers were designed with primer express software (Applied Biosystems) for the housekeeping genes β-actin ...
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bioRxiv - Neuroscience 2021Quote: ... The oligonucleotide primers were designed with primer express software (Applied Biosystems) for SNAP25 ...
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bioRxiv - Cell Biology 2021Quote: ... using forward primer 5’-CACAGGAAGCCCTGGAAGCT and reverse primer 5’-GAGCAGGTCAGGTTTTTGGA (Invitrogen). SIGLEC22P was amplified similarly with forward primer 5’-GCACCTCAGAGTGGAAGGAC and reverse primer 5’-GAAGGGGTGACTGAGGTACA ...
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bioRxiv - Cell Biology 2021Quote: ... with forward primer 5’-GCACTTGCAGCCGGATTTTTGGATCCATAGCCAGGGCC and reverse primer 5’-GGCCCTGGCTATGGATCCAAAAATCCGGCTGCAAGTGC (Invitrogen) to generate the pcDNA3.1-KIR-CD33-V5/HIS vector ...
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bioRxiv - Cancer Biology 2023Quote: ... The qPCR reaction was set up with 1 μl of diluted complementary DNA and 20X TaqMan probes and TaqMan Universal PCR Master Mix (Life Technologies, 4324018) in a 15 μl total volume ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0, ThermoFisher Scientific). For the data analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was synthesized with 100 ng of total RNA using specific stem-loop RT primers and TaqMan microRNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). Real-time RT-qPCR for miRNA was performed using TaqMan Universal PCR Master Mix II (Applied Biosystems ...
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bioRxiv - Biochemistry 2020Quote: ... MMLV RT (ThermoFisher) and MutiScribe™ RT (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... and reference 18S ribosomal RNA gene (Forward primer: 5′-TAGAGGGACAAGTGGCGTTC-3′, Reverse primer: 5′-CGCTGAGCCAGTCAGTGT-3′, Invitrogen custom primers) was independently amplified using thermocycling conditions as described in 58 ...
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bioRxiv - Molecular Biology 2019Quote: ... functional primers with the corresponding probes were tested by qPCR using a StepOne Real-Time PCR instrument (Applied Biosystems). The following reagents were added for a 20 μL reaction mixture ...
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bioRxiv - Molecular Biology 2020Quote: ... Nick primers were used with SYBR® Green on the Step One plus Real-time PCR system (Applied Biosystems) and normalized to the Ct values obtained within the surrounding ∼300 bp DNA region amplified with the flanking primers (Table S2) ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of each cDNA were analyzed by real-time PCR with specific primers and PowerUp SYBR Green (Invitrogen) in an ABI QuantiStudio 3 real-time PCR system ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting cDNA samples were amplified by TaqMan quantitative PCR using commercially available primer/probe kits from Applied Biosystems for BDNF (Gene Expression Assay Mm00432069_m1 ...
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bioRxiv - Immunology 2021Quote: ... Targeted qPCR was performed using the QuantStudio 6 Real-Time PCR system using the following Taqman Primers (Applied Biosystems): BCL6 (Hs00153368_m1) ...
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bioRxiv - Neuroscience 2020Quote: ... reacted with one of the PCR primers and the BigDye terminator v3.1 cycle sequence kit (Thermo Fisher Scientific, 4337455), and the gene sequence was identified using a 3130 Genetic Analyzer (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... PR1 expression was detected using primers qPR1_F and qPR1_R by quantitative real-time PCR using Maxima SYBR green mix (Thermo Fisher) and the AriaMx Real-Time PCR system (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... cDNA (10 ng) was analyzed with gene-specific primers (Table S2) using the StepOnePlus realtime PCR system (Applied Biosystems) and FastStart Universal Probe Master (Roche Applied Science) ...
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bioRxiv - Molecular Biology 2022Quote: The primers used for real-time PCR were designed using PrimerExpress™ software (Applied Biosystems; see Supplemental Table S3). 800 ng total RNA were reverse transcribed to cDNA using High-Capacity cDNA Reverse Transcriptase kit (Ambion ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was amplified with two primers for each gene using Power SYBR Green PCR Master Mix (Applied Biosystems, USA) and Real-time PCR analysis was performed using QuantStudio 12K Flex (Applied Biosystems by Life technology ...
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bioRxiv - Immunology 2020Quote: ... using 10ng of cDNA and 200nM of each specific primer on a 7500 Fast Real-PCR system (Applied Biosystems). cDNA concentration in each sample was normalized to GAPDH expression ...
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bioRxiv - Microbiology 2021Quote: ... with gene-specific primers according to the manufacturer’s protocol and the Applied Bioscience StepOnePlus qRT-PCR machine (Life Technologies). The expression levels were normalized to the endogenous 18S rRNA expression using the delta-delta comparative threshold method (ΔΔCT) ...
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bioRxiv - Immunology 2022Quote: ... and gene specific primers according to manufacturer’s instructions on a Quantstudio 3 Real-Time PCR system (Thermo Fisher Scientific). CT values were averaged across technical replicates and normalized against RPS17 ...