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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... RT-PCR assays were performed in 384-well plates (Attendbio, Cerdanyola del Valles, Spain) using the 7900HT RT-PCR system (Thermo Fisher Scientific, Waltham, MA, USA) and the commercial reagents Premix Ex Taq™ (BN2001 TaKaRa Bio Inc. ...
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bioRxiv - Microbiology 2022Quote: ... and quantitative RT-PCR was completed using Power SYBR Green PCR Master Mix in a QuantiStudio™ 5 thermocycler (Applied Biosystems, Foster City, CA, USA) to evaluate the mRNA levels of the following genes using the primers listed in Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription and first round polymerase chain reaction (PCR) were performed using a SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase kit (Invitrogen, Carlsbad, CA, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Thermo Fisher) and the Applied BiosystemsTM ViiA Real-Time PCR Systems by relative quantitative RT-PCR (Thermo Fisher Scientific, Waltham, MA; Applied Biosystems, Foster City, CA) and expression fold change was calculated using the ΔΔCq calculation method ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR was then performed using the Applied Biosystems StepOne™ system with SYBR Green RT-PCR Master Mixes (Thermo Fisher Scientific, cat: #A25742). The PrimePCR SYBR green assay primers (see Table 4 ...
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bioRxiv - Molecular Biology 2024Quote: ... 2μg RNA and HvHsfA6a forward and reverse primers were taken for cDNA synthesis and PCR amplification of 1119 bp ORF of HvHsfA6a gene was performed by using one step SuperscriptII RT-PCR system (Invitrogen, Life Technologies, Rockville, MD, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Gene expression was measured by RT-qPCR which was performed using either KiCqStart® SYBR® Green Primers (sigma-aldrich, UK) or TaqMan™ Gene Expression Assay (Thermofisher, UK) as indicated ...
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bioRxiv - Developmental Biology 2023Quote: Expression of Rspo1 and Rspo3 in the embryonic skin mesenchyme was analysed with primer-based RT-qPCR using Fast-SYBR Green Master Mix (Thermo Fisher Scientific, Vilnius, Lithuania) in 20 μl reactions with 15 ng template and 0.5 µM of each primer ...
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bioRxiv - Plant Biology 2023Quote: ... was used to extract total RNA from plant tissues and around 1.5 µg of DNAseA treated RNA was taken for cDNA synthesis using oligo-dT and random hexamers mix as primers (Invitrogen - SuperScript III RT kit). The cDNA template was used for qPCRs using SYBR green (Solis Biodyne – 5x HOT Firepol Evagreen qPCR master mix ...
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bioRxiv - Cell Biology 2020Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). The primer sequence for specific transcripts were as followed ...
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bioRxiv - Cell Biology 2020Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). The primer sequence for specific transcripts were as followed ...
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bioRxiv - Cell Biology 2020Quote: ... 100 nM of each primer and 10 μl 2x SYBR® Green PCR Master Mix (Applied Biosystems™). qPCR cycles consisted of the initial cycle of 50°C for 2 minutes and the second cycle of 95°C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... with 0.8 μM of forward and reverse primers on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) with the following cycling conditions ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR was performed using PrimeTime primers and probes (Integrated DNA Technologies) and TaqMan MasterMix (ThermoFisher/ Applied Biosystems) in the ViiA7 Real-Time PCR System (ThermoFisher/ Applied Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... Quantitative PCR was performed using PrimeTime primers and probes (Integrated DNA Technologies) and TaqMan MasterMix (ThermoFisher/ Applied Biosystems) in the ViiA7 Real-Time PCR System (ThermoFisher/ Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... Gene-specific primers were used to perform qPCR on the ABI 7500 Real-Time PCR System (Applied Biosystems) with MESA GREEN qPCR MasterMix Plus and SYBR® Assay Low ROX (Eurogentec ...
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bioRxiv - Evolutionary Biology 2019Quote: ... optimizing the primer pairs and concomitant PCR protocol for our own populations using Dreamtaq polymerase (Thermo Fisher Scientific). Each primer pair was amplified individually in 15μL reactions wherein each reaction contained 3μL 5 X Dreamtaq buffer ...
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bioRxiv - Cell Biology 2019Quote: ... the CEN2 CDS was amplified using primers S3 and AS3 and cloned into pCR-Blunt-II-TOPO (Invitrogen), prior to ligation with the plasmid backbone of pmin-mCherryFP-TgICMAP1 via the BamHI and AflII sites ...
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bioRxiv - Systems Biology 2020Quote: ... Primers were resuspended in 15 μL PCR master mix (0.2 μL Phusion High Fidelity DNA Polymerase (Thermo Scientific), 0.4 μL 10 mM dNTP Solution (New England Biolabs) ...
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bioRxiv - Systems Biology 2020Quote: ... 200 nM of each forward and reverse primer and 1X Power SYBR Green PCR Master Mix (Applied Biosystems). The real-time PCR program consisted of 1 cycle of 95°C for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR amplified using primers containing the SpeI and AsiSI cut sites and TOPO-TA cloned into PCR2.1 (Invitrogen) and inserted into the pCS2+8CeGFP (Gökirmak et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... Low ROX (Quantabio) and specific primers on a 7500 Fast Real Time PCR System (Applied Biosystems, Life Technologies). Amplification was performed at 95 °C for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Low ROX (Quantabio) and specific primers on a 7500 Fast Real Time PCR System (Applied Biosystems, Life Technologies). Amplification was performed at 95 °C for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and gene specific primer pairs (see Supplemental Table 1) with the 7300SDS Real-Time PCR System (Applied Biosystems). The ΔΔCt method was used to calculate relative expression values ...
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bioRxiv - Cancer Biology 2022Quote: ... Low ROX (Quantabio) and specific primers on the 7500 Fast Real Time PCR System (Applied Biosystems, Life Technologies). Amplification was performed at 95 °C for 10 min ...
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bioRxiv - Physiology 2019Quote: HindIII and BamHI restriction sites on the primers were used to clone the PCR product into pcDNA3.1(+) (Invitrogen). Sequencing of the entire ORF confirmed the sequence was identical to NCBI RefSeq NM_018252.2 ...
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bioRxiv - Immunology 2021Quote: ... using plasmid specific primers on StepOne™ Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific, Lithuana). Plasmid DNA at different concentrations (1 x 102 to 1 x 107 copies/mL ...
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bioRxiv - Immunology 2021Quote: ... using plasmid specific primers on StepOne™ Real-Time PCR System (Applied Biosystems by Thermo Fisher Scientific, Lithuana). Plasmid DNA at different concentrations (1 x 102 to 1 x 107 copies/mL ...
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bioRxiv - Genetics 2020Quote: ... Genotyping PCR primer sequences were designed utilizing the aid of IDTdna PrimerQuest tool and were manufactured by Invitrogen. Two such deletion mutants were isolated as heterozygotes and later homozygosed to obtain gst-4 (lp10 ...
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bioRxiv - Neuroscience 2021Quote: ... Primers are reported in Table 3 and qPCR was performed with SYBR green PCR master mix (Applied Biosystems). Samples were excluded if technical replicates failed ...
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bioRxiv - Immunology 2021Quote: ... PCR was performed by using the forward Xf and reverse Xr primers and Accuprime Pfx DNA polymerase (Invitrogen) and the PCR products ...
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bioRxiv - Cancer Biology 2022Quote: ... Low ROX (Quantabio) and specific primers on the 7500 Fast Real Time PCR System (Applied Biosystems, Life Technologies). Amplification was performed at 95 °C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... PCR primers were purchased desalted and resuspended in Tris-EDTA (TE) buffer at 20 µM concentration from Invitrogen. Pairs of primers were combined and diluted with Milli-Q water to a concentration of 1.25 µM (each primer) ...
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bioRxiv - Microbiology 2023Quote: ... and then primers listed in Supplemental Table 2 on a ViiA 7 real-time PCR system (Applied Biosystems) with the following cycling parameters ...
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bioRxiv - Molecular Biology 2023Quote: ... with primers for RNAs of interest (Supplementary Table S1) on a QuantStudio Real-Time PCR system (Thermo Fisher). Relative levels were quantified using the ΔΔCt method73.
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bioRxiv - Genetics 2023Quote: ... 0.5 µM of reverse primers (Supplementary Table) and 1X Power SYBR Green PCR Master Mix (Thermo Fisher, #4367659) in a 384-well plate (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... 0.15 μM of each primer and 125 μg cDNA using a StepOneTM Real-Time PCR System (Applied Biosystems). PCR conditions were ...
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bioRxiv - Cell Biology 2023Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). The relative levels of gene transcripts were normalized to GAPDH which were determined by quantitative real-time PCR ...
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bioRxiv - Cell Biology 2023Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). The relative levels of gene transcripts were normalized to GAPDH which were determined by quantitative real-time PCR ...
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bioRxiv - Cancer Biology 2023Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). Amplification was carried out at 95 °C for 12 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and specific primers on a 7,500 fast real-time PCR system (Applied Biosystems, Life Technologies, Waltham, MA, USA). Amplification was carried out at 95 °C for 12 min ...
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bioRxiv - Systems Biology 2023Quote: ... Primers were resuspended in 15 μL PCR master mix (0.2 μL Phusion High Fidelity DNA Polymerase (Thermo Scientific), 0.4 μL 10 mM dNTP solution (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 μL of cDNA was subjected to PCR with exon-specific primers using Platinum Taq DNA Polymerase (ThermoFisher). PCR reactions underwent 35 cycles of replication ...
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bioRxiv - Microbiology 2023Quote: ... The qPCR reaction mix contained 0.3μM of each primer into 10μL of Power SYBR Green PCR Master Mix (Applied Biosystems). The 284-well plate was filled using an Evo® TECAN robot and transferred into a qPCR machine (Thermofisher - QuantStudio6 ...
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bioRxiv - Cell Biology 2024Quote: Primers for qPCR (Table 1) were confirmed using standard PCR using DreamTaq master mix (Thermo Fisher, cat: K1082), 500 ng cDNA ...
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bioRxiv - Immunology 2021Quote: Primers (Invitrogen/ThermoFisher) and MGB-modified probes (Applied Biosystems/ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... Primer sequences (Invitrogen) used were ...
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bioRxiv - Biophysics 2021Quote: ... The primers (Invitrogen) designed and used for the site-directed mutagenesis are the following ...
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bioRxiv - Neuroscience 2019Quote: ... The primers (Invitrogen) were as follows ...
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bioRxiv - Immunology 2020Quote: ... Random primers (Invitrogen) and MMLV reverse transcriptase (Invitrogen ...