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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: cDNA synthesis for standard RT-PCR experiments was carried out using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems): 1 μg RNA dissolved in 10 μl H2O was added to 10 μl of the 2X Reverse Transcription Master Mix ...
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bioRxiv - Bioengineering 2020Quote: ... and RNA was reverse-transcribed using the SuperScript III First-Strand Synthesis System for RT-PCR (Thermo Fisher Scientific). The master mix included 2X SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... and RT-qPCR was performed in a QuantStudio Real- Time PCR system using SYBR green master mix (A25742, Thermofisher). The specificity of the amplification products was verified by melting curve analysis ...
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bioRxiv - Cell Biology 2021Quote: Total RNA extracted as above was subjected to quantitative RT-PCR analysis using the SYBR Green method (Applied Biosystems) and a pair of primers ...
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bioRxiv - Microbiology 2020Quote: ... I595K RNA was extracted via TRIzol LS extraction and subjected to RT-PCR using SuperScript IV (Invitrogen Life Technologies) as per the manufacturer’s instruction for sequencing.
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bioRxiv - Microbiology 2020Quote: ... I595K RNA was extracted via TRIzol LS extraction and subjected to RT-PCR using SuperScript IV (Invitrogen Life Technologies) as per the manufacturer’s instruction for sequencing.
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bioRxiv - Plant Biology 2020Quote: First-strand cDNAs were synthesized from total RNA using SuperScript First-strand synthesis system for RT-PCR kit (Invitrogen). Real-time qRT-PCR was performed using SYBR Premix EX TaqTM ⊓ (TAKARA ...
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bioRxiv - Microbiology 2021Quote: ... Conventional RT-PCR was performed using the Invitrogen™ SuperScript™ IV First-Strand Synthesis System (Thermo Scientific, USA) for cDNA synthesis and DreamTaq Green PCR Master Mix (2X ...
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bioRxiv - Physiology 2021Quote: ... Gel-purified RT-PCR products were then cloned into pCR4-TOPO vector using the TOPO TA cloning kit (Invitrogen), and automated DNA sequencing of cloned PCR products was performed using Big Dye chemistry (ABI 3730 capillary sequencer ...
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bioRxiv - Microbiology 2020Quote: ... Analyses were carried out on an Applied Biosystems 7500 RT–PCR system using the TaqMan Mix (ThermoFisher Scientific Inc) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: RT-qPCR was performed in a 96-well plate on the 7500 Fast Real-Time PCR System (Applied Biosystems). The cDNA amplification reactions contained ...
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bioRxiv - Microbiology 2020Quote: ... per manufacturer’s instructions and RT-qPCR was performed using the Power SYBR Green PCR master mix kit (Applied Biosystems). The MLV primers used were for the ecotropic MLV infections (5’-TACAGGGAGCTTACCAGGCA-3’/ 5’-GTTCCTATGCAGAGTCCCCG-3’ ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng of RNA was subjected to RT-PCR by using High Capacity RNA-to-cDNA kit (Life Technologies). The primers used for PCR:
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bioRxiv - Cancer Biology 2022Quote: ... in combination with TaqMan Gene Expression Assays per manufacturer protocol on a ViiA 7 RT-PCR system (Applied Biosystems). The assay IDs for TaqMan Gene Expression Assays used were as follows ...
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bioRxiv - Cancer Biology 2022Quote: ... First strand cDNA synthesis was generated using the Superscript III First-Strand Synthesis System for RT-PCR (Life Technologies) following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Two-hundred ng of cDNA were used for RT-PCR using pre-designed Taqman® Gene Expression Assays (Thermofisher). RT-PCRs were performed using the following specific assays (all Thermofisher) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher, Foster City, CA, USA) using GoTaq qPCR master mix (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed on the ViiA 7 Real-Time PCR System (Applied Biosystems/Thermofisher, Foster City, CA, USA) using GoTaq qPCR master mix (Promega ...
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bioRxiv - Cancer Biology 2020Quote: Reverse transcriptase polymerase chain reaction (RT-PCR) was performed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: ... We synthetized cDNA from 1μg of total RNA using Superscript III First-Strand Synthesis System for RT–PCR (Invitrogen) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR PCR was then performed using TaqMan® Gene Expression Assay FAM probes for TM6SF2 gene (Life Technologies). For TM6SF2 protein estimation ...
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bioRxiv - Microbiology 2020Quote: RT-qPCR was run on an a StepOne Real-time PCR System using SyberGreen Turbo Master mix (Applied Biosystems), in a total reaction volume of 25 μl with primers at 300 nM ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed in triplicate using the 7300 Real-time RT-PCR system (Applied Biosystems) according to the manufacturer’s description using the following thermocycler parameters ...
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bioRxiv - Immunology 2020Quote: ... Total RNA was reverse transcribed using the III first-strand synthesis system for RT-PCR (Invitrogen, Breda, the Netherlands). In short ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from 0.5 μg RNA templates using the SuperScript First-Strand Synthesis System for rt-PCR (Invitrogen) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2022Quote: ... The mRNA level of target genes was accessed by RT-qPCR on a Real-time PCR system (Applied Biosystems) using the SYBR green supermix reaction (Bio-Rad ...
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bioRxiv - Zoology 2022Quote: The first step RT-PCR reaction is performed in 12.5 μl mixture consisting of 1X Reaction Mix (Invitrogen, USA), 0.4 μM each of WzSV8-482F and WzSV8-482R primers (Table 1) ...
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bioRxiv - Plant Biology 2019Quote: ... The RT-qPCR was conducted in triplicates on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) with SYBR Green Master Mix (Vazyme Biotech) ...
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bioRxiv - Immunology 2019Quote: Full length Hem1 was PCR-amplified from cDNA prepped from T cells from a healthy control using Qiagen’s RNeasy kit and the SuperScript-III First Strand Synthesis System for RT-PCR (Invitrogen) using random hexamers and the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2019Quote: ... Nestin and TUBB3) were performed using real-time reverse transcriptase (RT-PCR) with SYBR Green master mix (Applied Biosystems) and analyzed by StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: Extracted total RNA was reverse transcribed into cDNA using SuperScript III first-strand synthesis system for RT-PCR (Invitrogen). Transcript levels of selected targets and the internal control ...
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bioRxiv - Molecular Biology 2020Quote: ... The first PCR reaction mix contained 0.2 µl of Super Script III RT/Platinum Taq Mix (Invitrogen; Cat# 2010520), 0.1 µl of Superase Rnase Inhibitor (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... RT-PCR was performed as a two-step real time rtPCR with SuperScript™ IV VILO™ from ThermoFisher, followed by a PCR as described elsewhere ([15] ...
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bioRxiv - Neuroscience 2019Quote: ... Quantitative RT-PCR was performed using the ABI PRISM 7900 sequencing detection system (Applied Biosystems, Foster City, CA, USA) with SYBR green master mix under the following conditions ...
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bioRxiv - Developmental Biology 2019Quote: ... and 0.5 μg (legs, pleuropodia) or 1 μg (pieces of embryos) of the RNA using ThermoScript RT-PCR System (Invitrogen) at 55°C ...
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bioRxiv - Neuroscience 2019Quote: ... RT-PCR was performed using TaqMan Gene Expression Assays following the TaqMan Fast Advanced Master Mix protocol (Applied Biosystems) on the QuantStudio 6 Flex instrument (Applied Biosystems).
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bioRxiv - Genetics 2020Quote: ... The Superscript III First Strand RT‐PCR kit was used to synthesize cDNA according to the manufacturer's protocol (Invitrogen). Real‐time qPCR was performed on LightCycler instrument (Roche ...
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bioRxiv - Genetics 2020Quote: ... RT-PCR was carried out on the ABI/Prism 7900 HT Sequence Detector System (Applied Biosystems, Carlsbad, CA, USA). Samples were tested by Real-time PCR using the following primers that were designed from Universal Probe Library Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... according to the manufacturer’s instructions and analysed by quantitative RT-PCR using the Fast SYBR Green Master Mix (ThermoFisher). Primers are listed in the Supplemental Methods Table S2.
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bioRxiv - Cell Biology 2021Quote: ... Five hundred micrograms of total RNA wasused for complementary DNA synthesis using Thermo Scientific RT–PCR kit (Thermo Scientific). Quantitative PCR was carried out using the Bio-Rad CFX machine (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Real-time RT-PCR was performed using the Power SYBR Green RNA-to-CT 1-Step Kit (Applied Biosystems) and an AriaMx Real-Time PCR system (Agilent).
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT-PCR analysis was performed using the Power SYBR Green RNA-to-Ct 1-Step kit (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was quantified by real-time RT-qPCR (EXPRESS One-Step Superscript™ qRT-PCR Kit, universal Invitrogen using 3.5µL of RNA and 6.5µL of RT qPCR mix and standard fast cycling parameters ...
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bioRxiv - Immunology 2021Quote: ... The S protein gene cDNA was amplified with a SuperScript IV One-Step RT-PCR kit (Thermo Fisher Scientific) using primers flanking the S gene ...
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bioRxiv - Immunology 2021Quote: ... Real-time RT-PCR was performed with Platinum SYBR Green qPCR SuperMix-UDG with ROX (Invitrogen, 11-744-500) and measured on StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2019Quote: ... reverse-transcribed according to the manufacturer’s protocol (SuperScriptIII One-Step RT-PCR system with Platinum Taq DNA Polymerase;Invitrogen, Cat# 12574-018 ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription was performed using total RNA and processed with the SuperScript first-strand synthesis system for RT-PCR according to the manufacturer’s protocol (Invitrogen). QRT-PCR was performed using the Applied Biosystems (Foster City ...
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bioRxiv - Neuroscience 2022Quote: ... To determine disruption of PFTK1 transcription the SuperScript III Platinum SYBR Green One-Step RT-PCR kit (Invitrogen, 204174) was used ...
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bioRxiv - Developmental Biology 2022Quote: ... A cDNA library was synthesized from 500 ng total RNA using SuperScript III First-Strand Synthesis System for RT-PCR using manufacturer’s instructions with random hexamers (Invitrogen). The primers used for this study are described in Supplementary File 1 ...
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bioRxiv - Cell Biology 2022Quote: ... Changes in gene expression were analyzed by quantitative RT-PCR using Fast SYBR-Green master mix (Thermo Fisher Scientific) on the ABI StepOne Plus Sequence Detection System (Applied Biosystems ...