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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... and carried out on an Applied Biosystems Vii 7 RT-PCR system (Thermo Scientific P/N 4453552). Validated gene-specific primers in this study can be found in Table S14 ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was synthesized using Superscript III First-strand Synthesis System for RT-PCR kit (Invitrogen, 18080-051) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated from 25-98°C in a QuantStudio Flex 12 RT-PCR machine (ThermoFisher Scientific). Measurements of fluorescence emission at 570nm were followed for each 0.3°C increment ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed as previously described using the Superscript III First-Strand Synthesis System (Thermo Fisher), iTaq universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR samples were run in duplicate on the StepOne Plus Real-Time PCR System (Applied Biosystems) at 95°C for 10 minutes and 60°C for 1 minute ...
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bioRxiv - Genetics 2022Quote: ... Reverse transcription of mRNA was performed using the SuperScriptIII First-Strand Synthesis System for RT-PCR (Invitrogen) with oligo(dT)20 primers ...
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bioRxiv - Developmental Biology 2023Quote: ... and a TaqMan Universal Master Mix II no UNG with a StepOnePlus RT PCR system (Applied Biosystems). Relative microRNA expression levels were normalized to RNU48 (assay ID ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using Power SYBR® Green PCR Master MIX (Applied Biosystems, Warrington, United Kingdom) and two specific primers (final concentration 500 nM each ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCRs were performed using an ABI550 Real-Time PCR machine (Applied Biosystems, Thermo Fisher Scientific) and the 2x SYBR Green qPCR Mix (Aidlab ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCRs were performed using an ABI550 Real-Time PCR machine (Applied Biosystems, Thermo Fisher Scientific) and the 2x SYBR Green qPCR Mix (Aidlab ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... reverse transcription polymerase chain reaction (RT-PCR) was performed utilizing SuperScript III (Thermo Fisher, Waltham, MA, USA). The reverse transcription process occurred at 58 °C for 50 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR reactions and were performed on a 7500 Fast Real-Time PCR System (Thermo Fisher Scientific). ELF1a was used as a reference gene to normalize transcript abundances ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was performed in triplicate using the PowerUP SYBR Green PCR master mix (Thermo Fisher Scientific) and the Bio-Rad CFX Opus 384 Real-Time RT-PCR systems ...
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bioRxiv - Physiology 2024Quote: ... We performed RT-qPCR in a 7500 Fast Real Time PCR System (Applied Biosystems, Foster City, CA) using TaqMan Fast Universal PCR Master Mix (Life Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... RT-qPCR was performed on a Step One Plus Real-Time PCR System (Thermo Fisher Cat#43765592R) with QuantiTect SYBR Green PCR Kit (QIAGEN Cat#204145) ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-qPCR was run using SYBR Green on an ABI ViiA7 real-time PCR system (Applied Biosystems), and results were normalized to the expression of an RPLP0 control primer set ...
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bioRxiv - Molecular Biology 2023Quote: ... was retro-transcribed to cDNA using RT-for-PCR kit (Invitrogen, Life technologies, Grand Island, NY, USA). The complete ORFs encoding CpomIR41a1 was amplified combining forward (5’-CACCATGATAATGCCAAGTAAAC-3’ ...
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bioRxiv - Immunology 2024Quote: ... using the AgPath-ID™ One-Step RT-PCR kit (Life Technologies, Zug, Switzerland, cat. no. AM1005) with vRNA segment 7-specific oligonucleotide primers and probe (50,51) ...
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bioRxiv - Immunology 2024Quote: ... Oligo-dT-primed cDNA was prepared using SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen). Poly(A ...
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bioRxiv - Cell Biology 2023Quote: ... 10µM forward and reverse primers were used for real time PCR in a StepOnePlus® Real-Time PCR system (all from Applied Biosystems, Waltham, MA, USA). The RT-qPCR amplification protocol comprised a polymerase activation step for 15 minutes at 95 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... with DNase set (ThermoFisher Scientific, 12185010). RNA quality was tested by Agilent Bioanalyzer and confirmed to have RIN numbers > 8.5 ...
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bioRxiv - Genetics 2019Quote: ... a Foxp3 buffer set (Invitrogen, US) was used according to manufacture instructions ...
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bioRxiv - Cell Biology 2020Quote: ... and BSA pre-set standards (ThermoFisher) to construct a standard curve ...
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bioRxiv - Microbiology 2023Quote: ... and dNTP Set (Thermo Scientific R0181), and qPCR was carried out using PowerUp SYBR Green Master Mix (Applied Biosystems A25741) ...
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Neuroprotective Effects of VEGF-B in a Murine Model of Aggressive Neuronal Loss With Childhood OnsetbioRxiv - Neuroscience 2023Quote: ... and the PureLinkTM DNase Set (Invitrogen). 1000 ng of RNA were converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... RT reaction with SuperScript IV RT (Thermo Fisher Scientific) was performed per manufacture’s recommendations with 150 ng of random primers (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to RT tubes (Maxima RT: Life Technologies, Inc. ...
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bioRxiv - Genetics 2022Quote: ... RT reactions were performed with Maxima RT (ThermoFisher Scientific), while qPCR reactions - with SensiFAST™ SYBR® No-ROX Kit (Bioline) ...
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bioRxiv - Genetics 2023Quote: ... RT reactions were performed with Maxima RT (ThermoFisher Scientific), while qPCR reactions were performed with SensiFAST™ SYBR® No-ROX Kit (Bioline) ...
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bioRxiv - Immunology 2021Quote: ... Specific primers were designed with Primer Express 2.0 (Applied Biosystems). The ORF1b primers used were as described before(29) ...
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bioRxiv - Plant Biology 2020Quote: Primers were designed using the Primer Express 3.0 (Life Technologies). Total RNA was extracted from Arabidopsis leaves using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Primers were designed (Primer Express, Applied Biosystems, Foster City, CA) validated ...
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bioRxiv - Immunology 2021Quote: ... Specific primers were designed with Primer Express 2.0 (Applied Biosystems). Primer sequences used for mRNA expression were for gene product ...
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bioRxiv - Neuroscience 2022Quote: ... The primers were designed using Primer Express Software (Applied Biosystems) at exon junctions to avoid genomic DNA amplification ...
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bioRxiv - Physiology 2022Quote: ... Primers were selected with Primer Express 3.0 software (Applied Biosystems).
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bioRxiv - Immunology 2023Quote: ... Specific primers were designed with Primer Express 2.0 (Applied Biosystems). The following primer sequences were used:
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Primers were designed by Primer Express software (Thermo Fisher Scientific) considering the exon-intron boundaries for all target genes (Table 1) ...
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bioRxiv - Molecular Biology 2024Quote: ... Primers were designed by Primer Express software 3.0 (Applied Biosystems) based on nucleotide sequences from the National Center for Biotechnology Information data bank ...
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bioRxiv - Physiology 2024Quote: ... Primers were designed using the Primer Express Software (Applied Biosystems). The TATA box binding protein (TBP ...
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bioRxiv - Neuroscience 2020Quote: ... A 20 µl PCR reaction was set up in each well using the SYBR PowerUp Green Master Mix (Applied Biosystems, USA) with 10ng of the converted cDNA and 0.3 M primers ...
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bioRxiv - Immunology 2022Quote: ... The reactions were set up using the manufacturer’s guidelines and run on a StepOnePlue Real-Time PCR Machine (Thermo Fisher Scientific). Values are represented as the difference in cycle threshold (Ct ...
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bioRxiv - Cell Biology 2019Quote: ... The incubation and cycling conditions were set as described in the kit and the plates were analysed in a StepOnePlus Real-Time PCR System (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... Reactions were set up in a 384-well plate and run in a QuantStudio 12K Flex Real-Time PCR System (4471081, Applied Biosystems) (2 min at 50C ...
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bioRxiv - Cell Biology 2021Quote: ... and gene specific primer sets for the core clock genes (Integrated DNA Technologies; see Table S1) on the StepOne Real-Time PCR system (Applied Biosystems). Housekeeping genes were determined by running samples from patients (Human geNorm kit ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative real-time PCR reactions were set up in technical triplicates with Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen, 11733-046) on a ViiA7 Real-Time PCR System (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR reactions set-up in a dedicated eDNA PCR hood used TaqMan Environmental Master Mix 2.0 (Applied Biosystems, Waltham, MA, USA). Reagent volumes and cycling conditions are listed in Table 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... A real-time PCR assay was set up using 25 μL of TaqMan Gene Expression Master Mix (ThermoFisher Scientific, California, USA), 17.8 μL of nuclease-free water (Ambion ...
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bioRxiv - Bioengineering 2023Quote: ... Relative gene expression qRT-PCR reactions were set up in 10-μL reaction mixtures containing TaqMan Universal Master Mix (Thermo Fisher), the appropriate set of primers ...
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bioRxiv - Biochemistry 2023Quote: ... A temperature ramp from 5°C to 95°C was set up at a speed of 0.02°C/s on a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific). All measurements were performed in triplicates ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR reactions were set up in technical triplicates with Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen, 11733-046) on a ViiA7 Real-Time PCR System (Thermo-Scientific) ...