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Citations for Thermo Fisher :
2151 - 2200 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... His-tagged AtKu70/80 was isolated from the supernatant using HisPur Ni-NTA resins (Thermo Fisher) and dialysed in 20 mM HEPES pH7.5 ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
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bioRxiv - Synthetic Biology 2021Quote: ... A melting curve was generated by monitoring fluorescence at 465 nm/610 nm excitation/emission wavelengths while increasing temperature from 10 °C to 90 °C at a rate of 0.06 °C/s on an Applied Biosystem ViiA 7 instrument (Life Technologies). To calculate Tm values ...
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bioRxiv - Bioengineering 2023Quote: ... The protein samples were heated from 25 °C to 95 °C at a rate of 0.3°C/sec in a StepOnePlus real-time PCR system (Applied Biosystems), and fluorescence was captured in the ROX channel ...
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bioRxiv - Microbiology 2021Quote: ... and incubated at 30 °C for 48 h in Bioscreen C (ThermoFisher), where the OD600 was measured at 1 h intervals ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-c-myc beads (Pierce Anti-c-Myc Agarose, Thermo Scientific), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... anti-c-myc beads (Pierce Anti-c-Myc magnetic beads, Thermo Scientific), and anti-HA beads (EZ view Red Anti-HA Affinity Gel ...
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bioRxiv - Immunology 2022Quote: ... vectors containing an Ig heavy chain gene were simultaneously transfected into Expi293 cells using Expi293 Expression System Kit (Thermo Fisher Scientific). Four days after the transfection ...
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bioRxiv - Immunology 2022Quote: ... In-house expressed antibodies were made through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Ig heavy chain genes were then PCR amplified separately with two rounds of nested PCR using DreamTaq Mastermix (Thermo Fisher Scientific). PCR products were Sanger sequenced by GenScript ...
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bioRxiv - Bioengineering 2019Quote: ... Human cardiomyocyte grafts were identified by staining for beta myosin heavy chain (βMHC) (hybridoma supernatant, ATCC #CRL-2046) and visualized using either Alexa Fluor-488 goat anti-mouse (1:100, Molecular Probes) or an avidin-biotin goat anti-mouse antibody (1:100 ...
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bioRxiv - Biochemistry 2019Quote: ... Appropriate correction factors for the individual TMT channels for both peptide N-terminal labelling and lysine side-chain labelling as per the TMT kits used (Thermo Scientific) were configured into the integrated Andromeda search prior to the MaxQuant search.
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bioRxiv - Genetics 2019Quote: Amplification was performed with real-time quantitative polymerase chain reaction (RT-qPCR) in a 25μL reaction mixture using the SYBR® Green PCR Master Mix (Applied Biosystems) and 0.2 μM of each primer ...
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bioRxiv - Genetics 2021Quote: ... The microsatellite marker Xpsp3000 was run using polymerase chain reaction (PCR) products and were separated on an ABI3730XL DNA fragment analyzer (Applied Biosystems), and alleles were scored with GeneMarkerv4.0 software (SoftGenetics) ...
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bioRxiv - Cell Biology 2020Quote: ... a sequence encoding the secretion signal from the V-J2-C region of the mouse Ig kappa-chain with hGAS6 was subcloned from pSecTag2-hGAS6-MycHis into pcDNA3.1(+) Mammalian Expression Vector (Thermo Fisher Scientific) using NheI/PmeI restriction sites.
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bioRxiv - Bioengineering 2021Quote: ... The reverse transcription and following polymerase chain reaction (PCR) was carried out using the RevertAid First Strand cDNA Synthesis kit (Thermo Scientific) and primers NL-1 and NL-4 (Table 1).
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bioRxiv - Biochemistry 2021Quote: ... (GenBank: ABA54613.1 and ABA54614.1) was expressed from heavy and light chain AbVec expression vectors in Expi293™ cells (Thermo Fisher Scientific). CR3022 supernatant was purified using HiTrap Protein G HP (Cytiva ...
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bioRxiv - Microbiology 2021Quote: Expression plasmids encoding the heavy and light chains of CV3-1 IgG or CV3-25 IgG were transiently transfected into Expi293F cells (Thermo Fisher) with ExpiFectamine 293 transfection reagent using the manufacturer’s protocol (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was prepared from 200 ng of total RNA using SuperScript® III First-Strand Synthesis System for real-time polymerase chain reaction (RT-PCR) (Cat. No. 18080-051, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Streptomycin-resistant colonies were screened for the chromosomal mutation of interest via colony polymerase chain reaction (PCR) using Taq DNA Polymerases (Thermo Fisher). Genomic DNA was purified from possible mutants and sequenced (Genewiz ...
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bioRxiv - Immunology 2020Quote: ... A pair of plasmids separately expressing the heavy- and light-chain of antibodies were transiently co-transfected into Expi293™ cells (Catalog No. A14528, ThermoFisher) with ExpiFectamine™ 293 Reagent ...
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bioRxiv - Cell Biology 2020Quote: ... Fifty nanograms of purified DNA was used for quantitative polymerase chain reaction (PCR) using TaqMan Genotyping Master Mix (Thermo Fisher Scientific) on an ABI7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2020Quote: ... This initial PEG treatment was followed by a secondary PEG treatment with 25mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific). These treatments resulted in a biotin-coated surface on the slide ...
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bioRxiv - Neuroscience 2022Quote: ... we cloned sequences encoding each BoNT light chain (from plasmids shared by Matt Kennedy, University of Colorado, USA) into the gateway vector donor plasmid (ThermoFisher, # K240020). We then transferred these sequences into the final pUASt destination vector from Carnegie Drosophila Gateway Vector Collection using the Gateway reaction kit (Thermofisher ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time polymerase chain reaction (RT-PCR) was performed on a 7500 Real-time PCR System (Applied Biosystems, Carlsbad, CA, USA) with Universal SYBR Green Master Mix (4913914001 ...
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bioRxiv - Cancer Biology 2022Quote: Mitochondrial DNA (mtDNA) copy number was determined by a quantitative polymerase chain reaction (qPCR) assay with ABI SYBR Green system (Applied Biosystems). 10 ng of total DNA from each cell was used as input for a 10 μL reaction.
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bioRxiv - Neuroscience 2021Quote: ... real-time polymerase chain reaction (qRT-PCR) was performed in replicate on a 7900HT Fast Real-Time PCR System (Life Technologies Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... specific real-time reverse transcriptase-polymerase chain reaction (RT-PCR) (TaqMan™ 2019-nCoV Assay Kit v2; Thermo Fisher Scientific, Italy) targeting RNA-dependent RNA polymerase was used to detect the presence of SARS-CoV-2 [16 ...
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bioRxiv - Immunology 2022Quote: ... pAb20-hCHIgG1 and pAb20-hCK plasmids discretely expressing heavy and light chain of antibodies were transiently co-transfected into Freestyle-293F cells (R79007, Thermo Fisher) employing purefection reagent (LV750A-1 ...
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bioRxiv - Immunology 2022Quote: ... pAb20-hCHIgG1 and pAb20-hCK plasmids separately expressing heavy and light chain of antibodies were transiently cotransfected into Freestyle-293F cells (R79007; Thermo Fisher) using purefection reagent (LV750A-1 ...
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bioRxiv - Immunology 2022Quote: ... The three Fabs were expressed by co-transfection of plasmids of heavy and light chain at the ratio of 2:1 in ExpiCHO expression system (Life Technologies) for two weeks according to the manufacturer’s manual ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... DNA consisting of 80-200 ng purified PCR product (see Polymerase chain reaction - sgRNA template preparation) was used in MEGAscript (Ambion, AM1330) or MAXIscript (Ambion ...
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bioRxiv - Microbiology 2019Quote: ... for fecal metabolites or a reverse phase octadecyl carbon chain bonded silica (C18) and an Accucore Vanquish C18+ UHPLC column (ThermoFisher Scientific) for serum metabolites ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... according to the manufacturer’s protocol using a StepOne Plus real-time polymerase chain reaction (RT-PCR) (Applied Biosystems, Foster City, CA). Relative gene expression of hypertension and vascular remodeling genes ...
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bioRxiv - Plant Biology 2019Quote: Expression studies were carried out by real-time reverse transcription polymerase chain reaction (RT-qPCR) using the StepOne plus thermocycler (Applied Biosystems), using the Power SyBR Green master mix (Applied Biosystems) ...
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bioRxiv - Bioengineering 2020Quote: ... The GFP fragment (942 bp) was produced by amplifying the pEGFP-C1 plasmid using polymerase chain reaction in the Proflex PCR system (ThermoFisher Scientific). The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.).
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (RTqPCR) was then performed using Taqman gene expression assays on a QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific). Gene expression was normalized to the placental reference gene Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta (YWHAZ ...
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bioRxiv - Immunology 2021Quote: ... Recombinant IgA was expressed without a J chain (to express only monomeric IgA) and purified using columns containing the CaptureSelect IgA Affinity Matrix (ThermoFisher Scientific). To produce antibody Fabs ...
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bioRxiv - Immunology 2021Quote: ... and fusion constructs of these antigens to the full length mouse invariant chain (CD74) (NCBI RefSeq: NC_000084.6) were obtained from GeneArt (Life technologies, Paisley, UK). The fusion Thyβ4-CD74 and Thyβ10-CD74 constructs were designed as an in-frame fusion of N-terminus of the thymosin cDNA sequences to the C-terminus of the full length murine invariant chain ...
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bioRxiv - Biochemistry 2020Quote: ... was used as a DNA template for a polymerase chain reaction (PCR) performed using Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific). Two amplification strategies were involved to obtain the template lacking an encoded polyA sequence (RNA G and H) ...
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bioRxiv - Genetics 2020Quote: The SNPrs2274907 was genotyped by polymerase chain reaction on a GeneAmp PCR system 9700 thermal cycler (Applied Biosystems, Foster City, CA) using the primers ...
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bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
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bioRxiv - Immunology 2020Quote: ... A pair of plasmids separately expressing heavy and light chain of antibodies were transiently co-transfected into Expi293™ cells (Catalog No. A14528, ThermoFisher) with ExpiFectamine™ 293 Reagent ...