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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was performed on an Applied Biosystems StepOnePlus™ using Fast SYBR (Thermofisher). The relative change in gene expression was analyzed in triplicate using the 2-ΔΔCT method47 with β-actin as the reference gene.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Complementary DNA (cDNA) was generated by reverse transcription polymerase chain reaction (RT-PCR) with SuperScript III (Thermo Fisher Waltham, MA USA). Reverse transcription was performed at 58°C for 50 min ...
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bioRxiv - Microbiology 2023Quote: ... Quantification of transcripts was carried out by quantitative reverse transcription polymerase chain reaction (RT-PCR) using SYBR Green PCR Master Mix (Applied Biosystems) in a QuantStudio 6 Flex Real-Time instrument (Applied Biosystems) ...
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bioRxiv - Physiology 2023Quote: ... Complementary DNA (cDNA) was generated by reverse transcription polymerase chain reaction (RT-PCR) with SuperScript III (Thermo Fisher Waltham, MA USA). Reverse transcription was performed at 58 °C for 50 min ...
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bioRxiv - Biochemistry 2023Quote: ... mNat2 mRNA expression levels were determined by quantitative polymerase chain reaction (qPCR) using the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). The cDNA was amplified using primers for mNat2 variant 2 and mGAPDH (see Supplementary Table S5 for details ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Complementary DNA (cDNA) was generated by reverse transcription polymerase chain reaction (RT-PCR) with SuperScript III (Thermo Fisher Waltham, MA USA). Reverse transcription was performed at 58 °C for 50 min ...
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bioRxiv - Microbiology 2023Quote: ... to be separated on 8% SDS-polyacrylamide gel electrophoresis (PAGE) or subjected to reverse transcriptase-polymerase chain reaction (RT-PCR) using Superscript IV (Invitrogen Life Technologies), to create cDNA and then DNA to amplify for sequencing.
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bioRxiv - Microbiology 2023Quote: ... with 127 μg each of heavy and light chain expression plasmids for the respective antibodies (kindly provided by Peter Kwong, Vaccine Research Center, NIH) using 293fectin (ThermoFisher Scientific) transfection reagent ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were stopped by addition of 1x LDS sample buffer and cleavage of Ub chains analyzed by SDS-PAGE and silver staining using the Pierce Silver stain kit (Thermo Fisher) according to the manufacturer’s instructions but skipping the initial wash step in water to avoid wash-out of monoUb.
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bioRxiv - Immunology 2023Quote: ... Real-time quantitative polymerase chain reaction (qRT-PCR) quantification of insulin mRNA and 18S rRNA levels was performed using TaqMan Gene Expression Assays (Applied Biosystems). Data for mRNA expression are provided as mean ± standard error of the mean of the estimated copy number ...
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bioRxiv - Immunology 2022Quote: ... Five μL from PCR reactions with heavy and light chain amplicons was mixed with 2 μL of ExoSAP-IT (ThermoFisher Scientific) and incubated at 37°C for 15 minutes followed by 80°C for 15 minutes to hydrolyze excess primers and nucleotides ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time quantitative polymerase chain reaction (qRT-PCR) was performed on an Applied Biosystems 7900HT system with SYBR green master mix (Applied Biosystems). Target mRNA levels were normalized using -β actin gene (Actb ...
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bioRxiv - Biophysics 2023Quote: ... then followed by a secondary PEG treatment with 25 mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific). A microfluidics chamber was constructed on the slide ...
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bioRxiv - Biophysics 2023Quote: We performed the biotinylation of single chain antibodies and of Gal-9 with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher). For this ...
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bioRxiv - Biophysics 2023Quote: ... The Pin1 coding region from exons 2 and 3 was recovered by amplifying each of these coding exons individually via polymerase chain reaction using the Phusion-Plus high-fidelity proof-reading enzyme (ThermoFisher Scientific). Primers used for amplification of each exon were as follows:
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bioRxiv - Synthetic Biology 2023Quote: ... Antibody-EndoTag fusions were then expressed via transient co-transfection of the EndoTag-heavy and light chains into Expi293F cells (Life Technologies) via PEI-MAX (Polyscience) ...
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bioRxiv - Bioengineering 2024Quote: Quantitative polymerase chain reaction: Isolated RNA was reverse transcribed into cDNA using the SuperScript™ II RT kit (Invitrogen, #18064-014) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmid p5E-vsx2 was generated by inserting a polymerase chain reaction (PCR)-amplified vsx2 promoter genomic fragment into p5E plasmid using BP clonase (ThermoFisher, 11789013). PCR reaction was performed using primers ...
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bioRxiv - Immunology 2024Quote: ... The IgM RhF gel band of the heavy and light chain was excised and digested with Pierce trypsin protease (ThermoFisher Scientific) and chymotrypsin (Promega ...
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bioRxiv - Immunology 2023Quote: ... Quantitative polymerase chain reaction(PCR) was conducted with PerfeCTa SYBR-Green FastMix-Rox(Quanta-Biosciences) and the StepOnePlus rt-PCR system(Applied Biosystems). We used the 2-ΔCT method for statistical analysis ...
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bioRxiv - Neuroscience 2024Quote: ... The real-time quantitative polymerase chain reaction (Real-Time qPCR) was performed using SYBR Green and the 7300 RealTimePCR system (Applied Biosystems). All data were analyzed using the delta-delta Ct approach ...
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bioRxiv - Neuroscience 2024Quote: ... The resulting cDNAs were diluted to 4 ng/µL and used as templates in real-time quantitative polymerase chain reactions (PCRs; QuantStudio3; Applied Biosystems). TaqMan oligonucleotide primers and probe sets (TaqMan Gene Expression Assays ...
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bioRxiv - Molecular Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze to relative expression levels using Quant Studio 5 from Applied Biosystems by Thermo Fischer Scientific (Foster City ...
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bioRxiv - Biochemistry 2024Quote: ... using a QuantStudio 3 Applied Biosystems real-time polymerase chain reaction (PCR) system with differential scanning fluorimetry capability (Thermo Fisher Scientific). Assays were repeated in triplicate in a 96-well 0.2-mL PCR-compatible plates ...
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bioRxiv - Microbiology 2024Quote: ... copy numbers were assessed with a quantitative reverse transcription polymerase chain reaction (RT-qPCR) using the Power Sybr Green RNA-to-Ct one step kit (Applied Biosystems), a dilution of synthetic CHIKV RNA as standard ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were co-transfected at a 1:2 heavy to light chain ratio into Expi293F cells using the Expifectamine 293 Expression Kit (Thermo Fisher), and antibodies were purified with protein A agarose (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated at 30 °C for 48 h in Bioscreen C (ThermoFisher), where the OD600 was measured at 1 h intervals ...
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bioRxiv - Cell Biology 2019Quote: ... and anti-c-myc beads (Pierce Anti-c-Myc Agarose, Thermo Scientific), respectively ...
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bioRxiv - Cell Biology 2019Quote: ... anti-c-myc beads (Pierce Anti-c-Myc magnetic beads, Thermo Scientific), and anti-HA beads (EZ view Red Anti-HA Affinity Gel ...
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bioRxiv - Microbiology 2019Quote: ... Anti-c-Myc (Invitrogen), Anti-double stranded J2 antibodies (Scicons).
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bioRxiv - Microbiology 2021Quote: ... at 4°C (Nunc MaxiSorp flat bottom ...
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bioRxiv - Immunology 2021Quote: ... C media (ThermoFisher Scientific) and plated onto Luria-Bertani
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bioRxiv - Genetics 2022Quote: ... Gateway Cassette C (Invitrogen) was cloned into the p413 vector just downstream of (3’ to ...
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bioRxiv - Developmental Biology 2023Quote: ... 37°C (ThermoFisher, AM1350). The mRNA was purified (RNeasy Mini kit ...
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bioRxiv - Cancer Biology 2020Quote: ... using Taqman probes (human RBM10: Assay ID, Hs00275935_m1, human Bcl-xL: Assay ID, Hs00236329_m1, Applied Biosystems, human Bcl-xS ...
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bioRxiv - Biochemistry 2022Quote: ... Human serum (catalog # 4522 from human male AB plasma) and fetal bovine serum were from Gibco, Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... and plasma human glucagon was measured by the human glucagon ELISA kit (Thermo Scientific, Frederick, MD). Exendin-4 plasma levels were measured using the exendin-4 EIA kit (Phoenix pharmaceuticals ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 50 μL fresh FACS buffer and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Immunology 2022Quote: ... Total IgG in human sera was measured using a commercial human IgG ELISA kit (Fisher Scientific) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... All human CD8+ T cells were activated with anti-human CD3/CD28 dynabeads (11131D, Thermo Scientific) at a 1:1 cell-to-bead ratio and cultured in complete RPMI supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... 100 ng/mL Human Recombinant IL-4 and 250 ng/mL Human Recombinant GM-CSF (ThermoFisher) for six days at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... Secreted Aβ42 peptides were quantified using the Human Amyloid β1-42 human ELISA kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Fresh human BAL cells and frozen human PBMCs were stained with Aqua live/dead dye (Invitrogen) for 20min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Human Jurkat T cells and human THP-1 monocytes were cultured in RPMI 1640 medium (ThermoFisher), supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Fragments of human CCDC22 and CCDC93 and full-length human DENND10 were ordered as GeneStrings (ThermoFisher) with the gene codon optimized for E ...
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bioRxiv - Cell Biology 2023Quote: ... Purified human neutrophils and T cells were cultured in Human Plasma-Like Medium (HPLM; ThermoFisher Scientific) with 5% heat inactivated FBS supplemented ...
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bioRxiv - Immunology 2023Quote: ... Primary human T cells were thawed and activated with Human T-Expander CD3/CD28 Dynabeads (Gibco) at a 3:1 bead:cell ratio in complete medium (RPMI 1640 supplemented with 10% fetal bovine serum ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 50 µL fresh FACS buffer and 10 µL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured as temperature increased 1°C/minute from 25°C to 95°C in a StepOne Plus qPCR instrument (Applied Biosystems). Protein from 2 independent purifications was used ...