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Citations for Thermo Fisher :
2051 - 2100 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... This was used as a polymerase chain reaction (PCR) template to amplify and produce [Platinum SuperFi PCR master mix (Invitrogen)] the NaTrxhΔNα(+3 ...
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bioRxiv - Bioengineering 2023Quote: ... quantitative polymerase chain reaction (qPCR) was performed using a QuantStudio 7 Flex qPCR system (4485701; ThermoFisher Scientific, Waltham, MA, USA) and PowerTrack SYBR Green Master Mix (A46109 ...
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bioRxiv - Synthetic Biology 2023Quote: ... (2011) [34] for the cassette assembly by polymerase chain reaction (PCR) using Platinum SuperFi Master Mix (Thermo Fisher Scientific, Lithuania) following the manufacturer’s instruction for high GC content-templates ...
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bioRxiv - Cell Biology 2023Quote: Knockdown of BubR1 and Dynein heavy chain (DHC) was achieved by transfecting 150 pM siRNA using Lipofectamine RNAiMax (Thermo fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: We generated immunoglobulin heavy chain (IGH) DNA libraries from complementary DNA generated from 10-500 ng of total RNA using Superscript III (Invitrogen) reverse transcriptase and random hexamer primers ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantified by real-time reverse transcriptase polymerase chain reaction (qPCR) using TaqMan technology (Hs00248808_m1) according to the manufacturers protocol (Applied biosystems). Each assay was repeated in triplicate.
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bioRxiv - Physiology 2024Quote: ... TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR) were then performed using a QuantStudio 7 Pro Real-Time PCR System (ThermoFisher). The housekeeping gene Vinculin was used as an internal control for cDNA quantification and normalization of gene amplified products.
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bioRxiv - Microbiology 2023Quote: ... antibody heavy and light chain variable domains were amplified with two rounds of nested PCR using Platinum II Hot-Start polymerase (Invitrogen). The resulting PCR products were assessed using 96w E-gels (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed on a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems) with the Power SYBR Green PCR Master Mix (Applied Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: ... DAPI (4′-6-diamidino-2-phenylindol) and Myosin Heavy Chain (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™). All images were taken using a confocal microscope (Zeiss LSM 780 Airyscan ...
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bioRxiv - Cell Biology 2024Quote: ... Polymerase chain reactions (PCR) were carried out using Phusion High-Fidelity DNA polymerase or DreamTaq DNA polymerase (Thermo Fisher Scientific). DNA fragments were ligated using T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the heavy chain and light chain of the antibody were transfected at an equimolar ratio into Expi293F cells using ExpiFectamine 293 transfection kit (Gibco) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Reverse transcription and subsequent PCR amplification of heavy and light chain variable genes were performed using SuperScript III (Life Technologies) as described previously27 ...
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bioRxiv - Cell Biology 2020Quote: ... thermoblocks at 90°C and 50°C (e.g., Thermo Fisher, 88870004), an ultracentrifuge capable of spinning 50 ml falcon tubes at 10,000 rpm (Beckman Coulter Avanti J-30 I ultracentrifuge and a Beckman JA-12 fixed angle rotor) ...
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bioRxiv - Neuroscience 2024Quote: ... Fast SYBR™C Green Master Mix (Applied Biosystems™C) was employed along with primers at a concentration of 5nM ...
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bioRxiv - Cancer Biology 2023Quote: ... and patient-derived cell cultures (P022.C, P024.C, P030.C) were cultured in Dulbecco’s modified Eagle medium (DMEM, #41966, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... The protein denaturation was monitored by obtaining the fluorescence signal by increasing the temperature from 22 °C-95 °C at 0.5 °C/minute rate using QuantStudio 3 real-time PCR (ThermoFisher). The melting temperature (Tm ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... rat anti-human (16G6) and rabbit anti-human polyclonal CD49a were all purchased from ThermoFisher. For collagen measurements 10 mg of allograft tissue/sample was analyzed with a Hydroxyproline Assay Kit (Sigma ...
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bioRxiv - Pathology 2022Quote: ... Aβ42 human ultrasensitive ELISA Kit and Tau (phospho) [pT231] human ELISA Kit from Thermo Fisher, and sAPPα and sAPPβ ELISA Kit from Mybiosource ...
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bioRxiv - Genomics 2021Quote: ... surviving human leucocytes in thawed samples were removed using anti-human CD45 DynaBeads (ThermoFisher Scientific). The resulting parasite pellet was washed to remove soluble human DNA (hDNA) ...
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bioRxiv - Genomics 2022Quote: Human total RNA was extracted from THP-1 human cells using TRIzol (ThermoFisher, cat# 15596026) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... human kinome panel (Cat # 4475388) and human stem cell openarray panel (Cat # 4475390, ThermoFisher, USA). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... A region spanning 2163 bps upstream from the transcription start site was PCR amplified using genomic DNA isolated from HEK293 cells with Platinum SuperFi II DNA polymerase (Thermo Fisher Scientific). The PCR product was cloned into pGL3 basic vector following conventional protocols ...
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bioRxiv - Cell Biology 2020Quote: Samples prepared following immunoprecipitation from HEK293 MceC3XFLAG cell line were analysed by LC-MS on an Orbitrap Elite™ mass spectrometer (ThermoFisher Scientific) coupled to a Dionex Ultimate 3000 Ultra-Performance Liquid Chromatography (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Phoenix cells were transfected with the pBabe plasmid DNA encoding for the protein of interest using Lipofectamine 3000 (Thermo Fisher Scientific) per manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2019Quote: ... Transfection of Flp-In T-Rex HEK293 cells was carried out according to Flp-In™ T-REx™ Core Kit (Invitrogen). Briefly ...
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were transfected with pcDNA3.1-GAS6-MycHis linearized with PvuI restriction enzyme using Lipofectamine® 2000 Transfection Reagent (Thermo Fisher Scientific), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The P2 baculovirus produced in Sf9 cells was added to HEK293 GnTI- cells (mycoplasma test negative, ATCC #CRL-3022) and grown in suspension in FreeStyle medium (GIBCO-Life Technologies) supplemented with 2% FBS at 37°C and 8% CO2 ...
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bioRxiv - Biochemistry 2020Quote: pcDNA5-based constructs for the expression of C-terminally His6-PreScission protease cleavage site-2xFlag (Flag)-tagged full-length or N-terminally truncated ASCC3 variants were transfected into HEK293 Flp-In™ T-REx™ cells (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Linearized plasmids containing the ItypOrco gene were transfected into HEK293 cells containing a tetracycline-inducible repressor (TREx) using Lipofectamine 2000 (Thermo Fisher Scientific) (Corcoran et al ...
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bioRxiv - Microbiology 2020Quote: ... HEK293-EBV B(+/−) S13+/− cells were transfected with pCDNA3-BZLF1 and pCDNA3-BALF4 using TurboFect Transfection Reagent (Thermo Scientific, Waltham, MA, USA). Old medium was changed with fresh medium in one day post transfection ...
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bioRxiv - Immunology 2021Quote: HEK293 adherent cells grown to a confluency of 70–90% were transfected using a Lipofectamine 3000 Reagent Kit (L3000075, Invitrogen, Waltham, MA). Transfection procedures were adapted from the product manual ...
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bioRxiv - Microbiology 2022Quote: ... Titration of progeny viruses from all HEK293 cells was done in Vero-E6 cells cultured in 16-well chambered slides (Thermo Fisher Scientific), as described below ...
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bioRxiv - Genetics 2022Quote: ... These plasmids were inserted into the same place in the genome using the Flp-In recombinase technology to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
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bioRxiv - Molecular Biology 2021Quote: HeLa cells and HEK293 cells (in 35-mm dishes) were transfected with 100 pmol siRNA using Lipofectamine RNAi max (Invitrogen–Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were grown in a monolayer on 10 cm culture dishes in Dulbecco’s Modified Eagle Medium (DMEM) (ThermoFisher Scientific, Waltham, MA) supplemented with fetal bovine serum (10% v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing either dCas9-KRAB or a targeting sgRNA were generated by transfection of HEK293 cells with Lipofectamine 2000 (Thermo Fisher Scientific). Cells were cotransfected with the packaging plasmid pCMV-dR8.91 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... HEK293 cells were a gift from Dr Harvey McMahon and were cultured in high-glucose DMEM supplemented with GlutaMAX (Thermo Fisher, 31966047) and 10% FBS (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: Cell lines were made by co-transfecting plasmids with lentiviral packaging vectors (VSVG and SPAX2) into packaging cells (HEK293-FT) using Lipofectamine 2000 (Thermo Fisher Scientific). Viral supernatants were collected ...
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bioRxiv - Biophysics 2021Quote: The HEK293 cell line subclone TS201A was a gift from Vladimir Yarov-Yarovoy and was maintained in DMEM (Gibco Cat# 11995-065) with 10% Fetal Bovine Serum (HyClone ...
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bioRxiv - Neuroscience 2022Quote: HEK293-T cells of low passage (<25) were kept in Dulbecco′s Modified Eagle′s Medium (DMEM, Thermo Fisher Scientific #31966-047) containing 10% fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2022Quote: ... 12 pmol crRNA:tracrRNA duplex was combined with 12 pmol recombinant HiFi Cas9 (IDT) and reverse transfected into HEK293 Flp-In cells using Lipofectamine CRISPRMAX reagent (Thermo Fisher Scientific) following IDT protocols ...
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bioRxiv - Genetics 2022Quote: ... pgLAP1-3MYC-BioID2 subnit plasmids were then transformed and stably integrated into HEK293-Flp-In-T-REx cells using the manufacturer’s protocol (ThermoFisher Scientific, catalogue #R78007).
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bioRxiv - Cell Biology 2022Quote: ... into the Flp-In™ T-REx™ HEK293 cell line or Jaw1 KO cells (#R78007; Thermo Fisher Scientific, Waltham, MA, USA). After incubation for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The mCherry tagged SETD6 WT or Y285A and EGFP-tagged E2F1 WT or K117R were co-transfected into HEK293 cells using polyethyleneimine (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Expression vectors of SLC22A10 orthologs were introduced into HEK293 Flp-In cells either through transient transfection or stable transfection using Lipofectamine LTX (Thermo Fisher Scientific). For transfections in a 48-well plate (seeding density ...
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bioRxiv - Physiology 2023Quote: ... mVenus:FL_ Proline and mVenus:2x linker proteins were transfected into HEK293 (Catalog CRL-1573, ATCC) cells with lipofectamine 3000 transfection reagent (Catalog L3000008, Thermo Fisher Scientific). 24h post-transfection ...