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Citations for Thermo Fisher :
2001 - 2050 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Human iPSCs (Thermo Fisher #A18945) were maintained in mTeSR1 medium (StemCell ...
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bioRxiv - Immunology 2022Quote: ... APRIL Human ELISA Kit (ThermoFisher) and Human CD83 DuoSet ELISA Kit (R&D systems) ...
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bioRxiv - Neuroscience 2022Quote: ... and human ACTB (Thermofisher Hs01060665_g1). For other qPCRs ...
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bioRxiv - Microbiology 2023Quote: ... human Gas6 (Invitrogen, cat# BMS2291), mouse Axl (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Human primary astrocytes (ThermoFisher #N7805200) were maintained as described in user manual ...
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bioRxiv - Systems Biology 2023Quote: ... and human IgE-PE (ThermoFisher). Antibody details are shown in Table S4 ...
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bioRxiv - Genomics 2023Quote: ... human p53 (Invitrogen, PA5-27822), MDM2 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... human IFN-gamma ELISA (Invitrogen) and granzyme B ELISA (R&D Systems ...
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bioRxiv - Physiology 2023Quote: ... Human GR-specific siRNA (Invitrogen) was used at a concentration of 100 nM for 48 h to effectively knockdown GR ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD235a (#17998742, Invitrogen), anti-human CD326 (EpCAM ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD45 (#17945942, Invitrogen), anti-human CD235a (#17998742 ...
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bioRxiv - Cell Biology 2023Quote: ... Human Cot-1 DNA (Invitrogen), 3M NaAc and 100% cold EtOH ...
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bioRxiv - Immunology 2024Quote: ... or anti-human (Invitrogen #A11013) secondary antibodies at 20 μg/mL in PBS/2% BSA for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... human COT1 DNA (#15279011, Invitrogen) and 3 volumes of ethanol (#10000652 ...
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bioRxiv - Neuroscience 2021Quote: Primary neurons expressing lentivirally delivered clathrin heavy chain (CHC)-targeting shRNA or a scramble version (DIV14) were starved for 1 hour in osmolarity-adjusted NBA medium (Gibco) at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed on a QuantStudio 12K Flex system (Applied Biosystems; Foster City, CA) using rat-specific Taqman gene primers (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... quantitative real time polymerase chain reaction (qPCR) was performed using PowerUp SYBR Green Master Mix kit according to the manufacturer’s protocol (Applied Biosystems) and LightCycler 480 II machine (Roche Applied Science) ...
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bioRxiv - Immunology 2021Quote: Multiplex quantitative polymerase chain reaction (qPCR) was carried out using a QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) as described previously [23] ...
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bioRxiv - Zoology 2019Quote: Gene expression levels were analysed with quantitative polymerase chain reaction (qPCR) using a StepOnePlus Real-Time PCR System (Applied Biosystems) as described previously [25] ...
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bioRxiv - Systems Biology 2020Quote: Pooled single cell samples were prepared for multiplex real-time quantitative polymerase chain reaction (RT-qPCR) with the Biomark HD system using VILO III (ThermoFisher) reverse transcription of RNA into cDNA followed by 22 cycles of pre-amplification (Taqman pre-amp master mix ...
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bioRxiv - Neuroscience 2020Quote: ... The prepared RNA was assayed for MALAT1 and cyclophilin A levels using primer/TaqMan probe sets with an EXPRESS One-Step SuperScript quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) kit (Invitrogen). qRT-PCR plates were run on StepOnePlus Real-Time PCR machines (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... Pooled single cell samples were prepared for multiplex real-time quantitative polymerase chain reaction (RT-qPCR) with the Biomark HD system using VILO III (ThermoFisher) reverse transcription of RNA into cDNA followed by 22 cycles of pre-amplification (Taqman Pre-amp Master Mix ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids containing the Fab heavy chains and light chains were chemically co-transfected into an afucosylated ExpiCHO cell line using the ExpiFectamine CHO Transfection Kit (Gibco). After 9 days of transient expression ...
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bioRxiv - Bioengineering 2022Quote: ... mAbs were produced by transient transfection into Expi293F™ cells with equal amounts of paired heavy- and light-chain expression vectors using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (Thermo fisher). Five days post antibody expression ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was performed using the PowerUp SYBR Green Master Mix kit according to the manufacturer’s protocol (Applied Biosystems) and a LightCycler 480 II machine (Roche Applied Science) ...
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Cryptococcus neoformans capsule regrowth experiments reveal dynamics of enlargement and architecturebioRxiv - Microbiology 2021Quote: ... cells were spun down and washed with PBS then resuspended in 1mL blocking solution with either 1:500 Goat anti-Mouse IgM Heavy Chain Secondary Antibody conjugated to AlexaFluor 488 (Invitrogen), 1:500 Rabbit anti Mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... The first strand cDNA for the expressed H and L chain was synthesized using a commercially available Superscript III reverse transcription kit (Invitrogen). The 25 μL reverse transcription reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Physiology 2022Quote: ... Pre-designed real-time polymerase chain reaction assays were purchased for the following genes: TPit (Thermo Fisher Scientific 4331182 Hs00193027), SF1 (Thermo Fisher Scientific 4331182 Hs00610436) ...
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bioRxiv - Developmental Biology 2021Quote: ... All real-time polymerase chain reactions were run with Taq MAN Universal PCR Master Mix (Applied Biosystems, Foster City, CA) and with primers from Applied Biosystems on a 7900 HT Fast Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Immunology 2019Quote: ... 5μg filter-sterilized DNA (2.5μg each of heavy and light chain-encoding plasmids) was added to 1mL of Expi293 medium (ThermoFisher Scientific; A1435101) in a fresh tube ...
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bioRxiv - Immunology 2021Quote: ... The vector encoding a hexa-His tag fused to a single chain homotrimeric mLIGHT extracellular domain (G73-V239) connecting by two (GGGGS)4 linkers was transfected into Expi293 (Gibco) cells using the ExpiFectamine 293 transfection kit (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... These specimens were transported under cold chain and inoculated onto Vero CCL-81 which was maintained in Eagle’s minimum essential medium (MEM; Gibco, UK) supplemented with 10 per cent foetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: For the antibodies, plasmids encoding heavy and light chains of antibodies (CR3022, and SR1-SR5) were co-transfected into Expi293F cells (ThermoFisher) according to the manufacturer’s instructions for expression of antibodies ...
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bioRxiv - Immunology 2021Quote: ... expression vectors for the heavy and light chain were co-transfected in the ExpiCHO cell line (Thermo Fisher Scientific, USA) using the manufacturer’s protocol ...
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bioRxiv - Physiology 2021Quote: ... Quantitative real time polymerase chain reaction (qRT–PCR) was performed using TaqMan_ Gene Expression Assays (all Life Technologies, Darmstadt, Germany). TaqMan_Gene Expression Master Mix and the StepOnePlusTM Real-Time PCR System (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... paired heavy and light chain plasmids for each mAb were expressed in Expi293F cells (Cat# A14527, ThermoFisher Scientific, Waltham, MA) in a 96-well format ...
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bioRxiv - Immunology 2021Quote: ... Quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis was performed using the TaqMan Universal PCR Master Mix (Applied Biosystems) and cDNA derived from 40ng total RNA ...
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bioRxiv - Immunology 2020Quote: Reverse transcription and subsequent PCR amplification of heavy and light chain variable genes were performed using SuperScript III (Life Technologies). First and second PCR reactions were performed in 50 ml volume with 5ul of reaction product using PCR mixture (SMART-Lifesciences) ...
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bioRxiv - Immunology 2020Quote: ... Quantitative real-time polymerase chain reaction was performed using the ABI Prism 7000 Sequence Detection System (Life Technologies, Carlsbad, CA). Run method ...
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bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction and following allelic discrimination were performed on QuantStudio 5 Real-Time PCR System platform (Applied Biosystems).
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bioRxiv - Genetics 2020Quote: ... Barcodes were designed on DeenaBIO and synthesized by IDT (Coralville, Iowa, USA. Pre-polymerase chain reaction of the pooled sample and PCR amplification was done using ThermoFisher Phusion II master mix ...
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bioRxiv - Cancer Biology 2021Quote: ... MEFs stably expressing inducible Poly94Q-CFP chains were transduced with lentivirus and selected with hygromycin B (Thermo Fisher Scientific, 10687010). Polyglutamine aggregates were induced by addition of Doxycyclin (5 μg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... plasmids containing the light and heavy chain of 10C7 or a non-targeted control hIgG1 (cmAb) were transiently expressed in Expi293F suspension cells (ThermoFisher) using polyethylenimine reagent ...
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bioRxiv - Biophysics 2022Quote: Each DNA fragment encoding heavy chain variable regions (VH) and light chain variable regions (VL) of glCV30 were synthesized (IDT) and cloned into the pCEP4 vector (Invitrogen). DNA fragments of CH1-CH2-CH3 of the gamma heavy chain and CL of the kappa-type light chain were inserted into the VH and VL ...
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bioRxiv - Physiology 2022Quote: ... Atrial and ventricular essential light chains MLC1a and MLC1s/v were immunolabelled using anti-MYL4 (Thermo Scientific™, PA5-49205). The atrial regulatory light chain (MLC2a ...
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bioRxiv - Microbiology 2022Quote: ... The mNAbs were produced by transfection of Expi293 cells with equal amounts of matched heavy and light chain vectors using Expifectamine according to the manufacturer’s instructions (ThermoFisher Scientific). After 5 days of culture at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... for quantitative polymerase chain reaction (qPCR) at the manufacturer’s recommended settings in a StepOnePlus Real-Time PCR System (Applied Biosystems). The relative mRNA expression was calculated using the 2-ΔΔct method (Livak and Schmittgen ...
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bioRxiv - Synthetic Biology 2023Quote: ... eBlocks were amplified via polymerase chain reaction (PCR) with Phusion High-Fidelity PCR Master Mix (cat. no. F531L) from ThermoFisher Scientific and purified using Qiagen PCR clean-up kits (cat ...
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Induction of osteogenic differentiation of MSCs by GSK3β knockdown through GSK3β siRNAs transfectionbioRxiv - Molecular Biology 2023Quote: ... the synthesis of the first chain of total cDNA on RNA matrix was performed using RevertAid kit (Thermo Scientific, Germany) according to the manufacturers’recommendations.
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bioRxiv - Cell Biology 2023Quote: ... This was used as a polymerase chain reaction (PCR) template to amplify and produce [Platinum SuperFi PCR master mix (Invitrogen)] the NaTrxhΔNα(+3 ...