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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... we cloned sequences encoding each BoNT light chain (from plasmids shared by Matt Kennedy, University of Colorado, USA) into the gateway vector donor plasmid (ThermoFisher, # K240020). We then transferred these sequences into the final pUASt destination vector from Carnegie Drosophila Gateway Vector Collection using the Gateway reaction kit (Thermofisher ...
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bioRxiv - Neuroscience 2022Quote: ... Real-time polymerase chain reaction (RT-PCR) was performed on a 7500 Real-time PCR System (Applied Biosystems, Carlsbad, CA, USA) with Universal SYBR Green Master Mix (4913914001 ...
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bioRxiv - Cancer Biology 2022Quote: Mitochondrial DNA (mtDNA) copy number was determined by a quantitative polymerase chain reaction (qPCR) assay with ABI SYBR Green system (Applied Biosystems). 10 ng of total DNA from each cell was used as input for a 10 μL reaction.
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bioRxiv - Neuroscience 2021Quote: ... real-time polymerase chain reaction (qRT-PCR) was performed in replicate on a 7900HT Fast Real-Time PCR System (Life Technologies Corporation ...
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bioRxiv - Biochemistry 2020Quote: ... specific real-time reverse transcriptase-polymerase chain reaction (RT-PCR) (TaqMan™ 2019-nCoV Assay Kit v2; Thermo Fisher Scientific, Italy) targeting RNA-dependent RNA polymerase was used to detect the presence of SARS-CoV-2 [16 ...
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bioRxiv - Immunology 2022Quote: ... pAb20-hCHIgG1 and pAb20-hCK plasmids discretely expressing heavy and light chain of antibodies were transiently co-transfected into Freestyle-293F cells (R79007, Thermo Fisher) employing purefection reagent (LV750A-1 ...
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bioRxiv - Immunology 2022Quote: ... pAb20-hCHIgG1 and pAb20-hCK plasmids separately expressing heavy and light chain of antibodies were transiently cotransfected into Freestyle-293F cells (R79007; Thermo Fisher) using purefection reagent (LV750A-1 ...
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bioRxiv - Immunology 2022Quote: ... The three Fabs were expressed by co-transfection of plasmids of heavy and light chain at the ratio of 2:1 in ExpiCHO expression system (Life Technologies) for two weeks according to the manufacturer’s manual ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... DNA consisting of 80-200 ng purified PCR product (see Polymerase chain reaction - sgRNA template preparation) was used in MEGAscript (Ambion, AM1330) or MAXIscript (Ambion ...
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bioRxiv - Microbiology 2019Quote: ... for fecal metabolites or a reverse phase octadecyl carbon chain bonded silica (C18) and an Accucore Vanquish C18+ UHPLC column (ThermoFisher Scientific) for serum metabolites ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... according to the manufacturer’s protocol using a StepOne Plus real-time polymerase chain reaction (RT-PCR) (Applied Biosystems, Foster City, CA). Relative gene expression of hypertension and vascular remodeling genes ...
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bioRxiv - Plant Biology 2019Quote: Expression studies were carried out by real-time reverse transcription polymerase chain reaction (RT-qPCR) using the StepOne plus thermocycler (Applied Biosystems), using the Power SyBR Green master mix (Applied Biosystems) ...
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bioRxiv - Bioengineering 2020Quote: ... The GFP fragment (942 bp) was produced by amplifying the pEGFP-C1 plasmid using polymerase chain reaction in the Proflex PCR system (ThermoFisher Scientific). The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.).
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Bioengineering 2020Quote: ... Primary antibody (P-myosin light chain 2, cell signalling, 3671S, rabbit, 1:50) and rhodamine-phalloidin (Invitrogen, Thermo fisher, 1:500) diluted in 1% BSA in 1xPBS were added and incubated at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantitative real-time polymerase chain reaction (RTqPCR) was then performed using Taqman gene expression assays on a QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific). Gene expression was normalized to the placental reference gene Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta (YWHAZ ...
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bioRxiv - Immunology 2021Quote: ... Recombinant IgA was expressed without a J chain (to express only monomeric IgA) and purified using columns containing the CaptureSelect IgA Affinity Matrix (ThermoFisher Scientific). To produce antibody Fabs ...
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bioRxiv - Immunology 2021Quote: ... and fusion constructs of these antigens to the full length mouse invariant chain (CD74) (NCBI RefSeq: NC_000084.6) were obtained from GeneArt (Life technologies, Paisley, UK). The fusion Thyβ4-CD74 and Thyβ10-CD74 constructs were designed as an in-frame fusion of N-terminus of the thymosin cDNA sequences to the C-terminus of the full length murine invariant chain ...
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bioRxiv - Biochemistry 2020Quote: ... was used as a DNA template for a polymerase chain reaction (PCR) performed using Phusion High-Fidelity DNA polymerase (ThermoFisher Scientific). Two amplification strategies were involved to obtain the template lacking an encoded polyA sequence (RNA G and H) ...
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bioRxiv - Genetics 2020Quote: The SNPrs2274907 was genotyped by polymerase chain reaction on a GeneAmp PCR system 9700 thermal cycler (Applied Biosystems, Foster City, CA) using the primers ...
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bioRxiv - Biochemistry 2021Quote: ... Serum Fab concentration was measured 7 days post HDD by ELISA using a goat anti-kappa light chain antibody (ThermoFisher Scientific).
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bioRxiv - Immunology 2020Quote: ... A pair of plasmids separately expressing heavy and light chain of antibodies were transiently co-transfected into Expi293™ cells (Catalog No. A14528, ThermoFisher) with ExpiFectamine™ 293 Reagent ...
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bioRxiv - Molecular Biology 2021Quote: ... The qPCR-positive samples were amplified with the same primer pair and sequenced with the dideoxy chain-termination protocol on an ABI PRISM 3730xl Genetic Analyzer (Applied Biosystems) using the BigDye Terminator cycle sequencing kit (Perkin Elmer ...
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bioRxiv - Immunology 2021Quote: ... Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR) were performed on the 7500 Dx Fast thermal cycler (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: Real-time quantitative polymerase chain reaction (qPCR) was performed for each of the selected candidate genes using a Viia7 (Applied Biosystems) thermocycler ...
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bioRxiv - Biochemistry 2020Quote: ... Polymerase Chain Reactions (PCR) were performed using a BioRad T100 Thermocycler thermal Cycler using Phusion High-fidelity DNA Polymerase (Thermo Scientific). Streptococcus thermophilus LMG18311 harboring ICESt3 genomic DNA was used as template for PCR amplifications ...
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bioRxiv - Genomics 2020Quote: mtDNA copy number (mtDNA-CN) was determined utilizing a multiplexed real time quantitative polymerase chain reaction (qPCR) assay with ABI TaqMan chemistry (Applied Biosystems) as previously described[7] (see supplemental methods) ...
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bioRxiv - Immunology 2020Quote: ... 5 μL from the PCR reactions showing the presence of heavy or light chain amplicons was mixed with 2 μL of ExoSAP-IT (Thermo Fisher) and incubated at 37°C for 15 min followed by 80°C for 15 min to hydrolyze excess primers and nucleotides ...
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bioRxiv - Immunology 2021Quote: A pair of plasmids separately expressing the heavy- and the light-chain of antibodies were transiently co-transfected into Expi293™ cells (Catalog No. A14528, ThermoFisher) with ExpiFectamine™ 293 Reagent ...
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bioRxiv - Microbiology 2022Quote: For PDE12 and RNaseL gene expression studies quantitative polymerase chain reaction (qPCR) analysis was performed using TaqMan Gene Expression Assays (ThermoFisher, UK). Data were normalized to the endogenous housekeeping gene GADPH and fold change differences in expression relative to control cells were calculated using the Comparative ddCT method.
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mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: Plasmids carrying the antibody heavy and light chain of nAbs were used for transient transfection of Expi293F™ cells (Thermo Fisher) as previously described22 ...
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bioRxiv - Genetics 2022Quote: ... The promoter and recombinase DNA were then amplified by polymerase chain reaction (PCR) using Phusion High Fidelity master mix (Thermo Scientific) and inserted using Gibson Assembly (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: ... the coding sequence of IgG1 constant part of the heavy chain (Reference sequence GenBank: MH975516.1) was amplified by PCR with Phusion Flash High-Fidelity PCR Master Mix (F548, Thermo Scientific, USA) using the primers shown in Supp ...
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bioRxiv - Immunology 2022Quote: ... Recombinant antibodies were produced by cotransfecting paired heavy and light chain expression plasmids into Expi293F cells using ExpiFectamine 293 transfection reagents (ThermoFisher Scientific), purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... Real-time quantitative reverse transcription – polymerase chain reactions (RT-qPCR) were performed on the 7500 Dx Fast thermal cycler (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and bla-SHV) was carried out by conventional polymerase chain reaction (PCR) with PCR master mix (DreamTaq Green PCR Master Mix; Thermo Scientific) using specific primers (Table 1).
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bioRxiv - Immunology 2022Quote: ... Amplified linear DNA cassettes encoding monoclonal heavy and light chain genes were co-transfected into Expi293 cells in 96-well deep-well plates using the Expi293 Transfection Kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... and cDNA was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) analysis using SYBR green PCR master mix (cat#4309155, Thermofisher Scientific) with sequence specific primers ...
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bioRxiv - Microbiology 2022Quote: All samples were tested by quantitative reverse transcriptase polymerase chain reaction (RT-qPCR) with either the TaqPath COVID-19 Combo Kit from Thermofisher (MHome) or CDC Influenza SARS-CoV-2 Multiplex Assay (HIVE) ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time polymerase chain reaction was carried out using a fluorescent temperature cycler (TaqMan®; Applied Biosystems, Foster City, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The IgM RhF gel band of the heavy and light chain was excised and digested with Pierce trypsin protease (ThermoFisher Scientific) and chymotrypsin (Promega ...
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bioRxiv - Physiology 2024Quote: Immunoprecipitation of proteins without the contaminant of antibody heavy and light chain through bead antibody conjugation was carried out using the Pierce Co-IP kit (Invitrogen #26149). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting chains were then incubated overnight at room temperature with 10x molar excess of EZ-Link Maleimid-PEG2-Biotin (Thermo Scientific). The next day ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA levels were quantified by quantitative real-time polymerase chain reaction (qRT-PCR) using the following PCR primers on a ABI PRISM 7900HT Sequence Detection System (Applied Biosystems). Primer sequences ...
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bioRxiv - Immunology 2023Quote: ... The paired heavy and light chain plasmid DNA were co-transfected into 293i cells using ExpiFectamine™ (Life Technologies, Carlsbad, CA) by following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmid p5E-vsx2 was generated by inserting a polymerase chain reaction (PCR)-amplified vsx2 promoter genomic fragment into p5E plasmid using BP clonase (ThermoFisher, 11789013). PCR reaction was performed using primers ...
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bioRxiv - Developmental Biology 2024Quote: ... Reverse transcriptase-polymerase chain reaction (RT-qPCR) was performed using a reaction mixture containing PowerSYBR Green PCR Master Mix (4367659, Thermo Fisher) and primers (250 nM each) ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) was performed on an Applied Biosystems StepOnePlus™ using Fast SYBR (Thermofisher). The relative change in gene expression was analyzed in triplicate using the 2-ΔΔCT method47 with β-actin as the reference gene.
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bioRxiv - Synthetic Biology 2023Quote: ... Antibody-EndoTag fusions were then expressed via transient co-transfection of the EndoTag-heavy and light chains into Expi293F cells (Life Technologies) via PEI-MAX (Polyscience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) was performed using TaqMan reagents and primer-probes (Applied Biosystems, Foster City, CA). Gene expression was normalized to the control gene TATA box binding protein (Tbp).