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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for Inhibin beta C chain INHBC Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Biophysics 2022Quote: ... The His tagged RBD construct was purified from the culture supernatant of Expi293 cells (Thermo Fisher) that were transfected with the plasmid DNA using FectoPRO (Polyplus Inc.) ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Synthetic Biology 2024Quote: ... His-tagged proteins were purified using 0.2 mL HisPur™ Ni-NTA Spin Columns from ThermoFisher according to the manufacturer’s protocol with the exception of carrying out 4 washing steps ...
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bioRxiv - Microbiology 2024Quote: ... The digestion cocktail for RNase HI-negative samples was further supplemented with 120 U RNaseOUT (Invitrogen), while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... before preparation for sequencing with using Ion PGMTM Hi-QTM View Sequencing Kit (Thermo Fisher Scientific). The library was added to an Ion 318TM Chip Kit v2 BC (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Immunology 2021Quote: ... His tag) (BLPSN-0986P, BETA Life Sciences, Fairfield, NJ, USA) was immobilized on a 96-well plate (442404, MAXISORP F96 NUNC Immuno-plate ...
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bioRxiv - Genetics 2020Quote: ... and exposed to 1% 2-hydroxy-propyl-beta-cyclodextrin for 45min were labelled with 50 μg/ml DiI-LDL (Invitrogen) for 30 min at 4 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were then incubated for an addition 12 – 24 hrs with 1:400 mouse anti-beta III tubulin (Invitrogen, 480011), 1:300 sheep anti-ChAT (Abcam ...
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bioRxiv - Microbiology 2020Quote: Luminex profiling was performed on whole blood that was allowed to clot for 20 minutes and then spun down using a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... Luminex kits that were used are as follows a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
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bioRxiv - Pathology 2019Quote: The primary antibody used for immunofluorescent analysis in rehydrated sections included: beta-amyloid (36-6900; 1:100, Thermo Fisher Scientific); αB-crystallin (Ab151722 ...
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bioRxiv - Cancer Biology 2020Quote: ... The relative expression of each target gene represents an average of triplicates that are normalized to the transcription levels of beta-actin (Hs99999903_m1; ThermoFisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Beta-lactamase activity was determined at 390 nm (yellow) and 486 nm (red) using a VarioSkan plate reader (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... biotinylated amyloid peptides spanning the amyloid beta sequence (−5 to 45) were pre-incubated with NeutrAvidin (ThermoFisher Scientific, MA, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Pathology 2019Quote: ... Membranes were blocked with 2.5% BSA and incubated with primary antibodies: beta-amyloid (36-6900; 1:100, Thermo Fisher Scientific); anti-ZO-1 (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... and human phosphatidylinositol 4-phosphate 5-kinase type-1 beta (hPIP5KB; Uniprot #O14986) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). The gene encoding yeast Mss4 (Uniprot #P38994 ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The first injection contained either vehicle (0.9% saline) or the 11-beta hydroxylase inhibitor metyrapone (20 mg/kg) (Fisher Scientific) in saline solution ...
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bioRxiv - Immunology 2021Quote: The RBD was conjugated to CuMVTT using the cross-linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, MA) at 10-molar excess for 60 minutes at 23°C ...
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bioRxiv - Neuroscience 2022Quote: ... media was changed to KSR media (Knockout DMEM, 15% KOSR, 1x MEM-NEAA, 55 μM beta-mercaptoethanol, 1x GlutaMAX; Gibco) with doxycycline (2 μg/ml ...
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bioRxiv - Microbiology 2022Quote: IL-8 and IL-1β cytokines were quantified by ELISA using the Rabbit IL-1 beta ELISA Kit and Rabbit IL-8 ELISA Kit (Invitrogen). Aliquots of 2 mL of lung homogenate shred in saline solution were centrifuged during 30 min at 10 000 rpm and 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... Serial sections were processed for immunohistochemistry using the 6F/3D mouse monoclonal amyloid-beta antibody (1:50; ThermoFisher, Waltham, MA) to visualize amyloid plaque pathology.
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bioRxiv - Bioengineering 2022Quote: ... mouse INFB1 (FAM, Mm00439552_s1) and Mouse ACTB (Actin, Beta) Endogenous Control (FAM™ Dye/MGB probe, Non-Primer Limited) (Thermofisher) and Applied biosystems Taqman™ Fast Universal PCR Master Mix (2x ...
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bioRxiv - Plant Biology 2024Quote: ... according to the manufacturer’s protocol (RLT buffer supplemented with beta-merchaptoethanol was used) and residual genomic DNA was removed using the Turbo DNA-free kit (#AM1907, Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... containing a final concentration of 2.5% beta-mercaptoethanol and loaded onto Bolt 4-12% Bis-Tris gels (ThermoFisher Scientific; NW0412) at equivalent concentrations (15-20 µg for cFLIP knockout validation based on available lysates ...
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bioRxiv - Biochemistry 2023Quote: ... and human phosphatidylinositol 5-phosphate 4-kinase type-2 beta (hPIP4KB; Uniprot #P78356) were derived from codon optimized genes synthesized by GeneArt (Invitrogen). Gene sequences were subcloned into either bacterial ...
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bioRxiv - Microbiology 2024Quote: ... NP and 250 ng of pPolI plasmid encoding a mvRNA were transfected alongside 100 ng of plasmid expressing firefly luciferase from the IFN-beta promoter and 10 ng of plasmid expressing Renilla luciferase using Lipofectamine 2000 (Invitrogen) to the manufacturer’s specifications ...
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bioRxiv - Biochemistry 2024Quote: ... was serial diluted using a solution containing 0.1% FA in LCMS grade water (Pierce 85170) and 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902). A total of 10 microliters of each dilution ...
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bioRxiv - Synthetic Biology 2021Quote: ... A melting curve was generated by monitoring fluorescence at 465 nm/610 nm excitation/emission wavelengths while increasing temperature from 10 °C to 90 °C at a rate of 0.06 °C/s on an Applied Biosystem ViiA 7 instrument (Life Technologies). To calculate Tm values ...
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bioRxiv - Bioengineering 2023Quote: ... The protein samples were heated from 25 °C to 95 °C at a rate of 0.3°C/sec in a StepOnePlus real-time PCR system (Applied Biosystems), and fluorescence was captured in the ROX channel ...
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bioRxiv - Immunology 2022Quote: ... vectors containing an Ig heavy chain gene were simultaneously transfected into Expi293 cells using Expi293 Expression System Kit (Thermo Fisher Scientific). Four days after the transfection ...
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bioRxiv - Immunology 2022Quote: ... In-house expressed antibodies were made through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Ig heavy chain genes were then PCR amplified separately with two rounds of nested PCR using DreamTaq Mastermix (Thermo Fisher Scientific). PCR products were Sanger sequenced by GenScript ...
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bioRxiv - Bioengineering 2019Quote: ... Human cardiomyocyte grafts were identified by staining for beta myosin heavy chain (βMHC) (hybridoma supernatant, ATCC #CRL-2046) and visualized using either Alexa Fluor-488 goat anti-mouse (1:100, Molecular Probes) or an avidin-biotin goat anti-mouse antibody (1:100 ...
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bioRxiv - Biochemistry 2019Quote: ... Appropriate correction factors for the individual TMT channels for both peptide N-terminal labelling and lysine side-chain labelling as per the TMT kits used (Thermo Scientific) were configured into the integrated Andromeda search prior to the MaxQuant search.
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bioRxiv - Genetics 2019Quote: Amplification was performed with real-time quantitative polymerase chain reaction (RT-qPCR) in a 25μL reaction mixture using the SYBR® Green PCR Master Mix (Applied Biosystems) and 0.2 μM of each primer ...
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bioRxiv - Genetics 2021Quote: ... The microsatellite marker Xpsp3000 was run using polymerase chain reaction (PCR) products and were separated on an ABI3730XL DNA fragment analyzer (Applied Biosystems), and alleles were scored with GeneMarkerv4.0 software (SoftGenetics) ...
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bioRxiv - Cell Biology 2020Quote: ... a sequence encoding the secretion signal from the V-J2-C region of the mouse Ig kappa-chain with hGAS6 was subcloned from pSecTag2-hGAS6-MycHis into pcDNA3.1(+) Mammalian Expression Vector (Thermo Fisher Scientific) using NheI/PmeI restriction sites.
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bioRxiv - Bioengineering 2021Quote: ... The reverse transcription and following polymerase chain reaction (PCR) was carried out using the RevertAid First Strand cDNA Synthesis kit (Thermo Scientific) and primers NL-1 and NL-4 (Table 1).
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bioRxiv - Biochemistry 2021Quote: ... (GenBank: ABA54613.1 and ABA54614.1) was expressed from heavy and light chain AbVec expression vectors in Expi293™ cells (Thermo Fisher Scientific). CR3022 supernatant was purified using HiTrap Protein G HP (Cytiva ...
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bioRxiv - Microbiology 2021Quote: Expression plasmids encoding the heavy and light chains of CV3-1 IgG or CV3-25 IgG were transiently transfected into Expi293F cells (Thermo Fisher) with ExpiFectamine 293 transfection reagent using the manufacturer’s protocol (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was prepared from 200 ng of total RNA using SuperScript® III First-Strand Synthesis System for real-time polymerase chain reaction (RT-PCR) (Cat. No. 18080-051, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Streptomycin-resistant colonies were screened for the chromosomal mutation of interest via colony polymerase chain reaction (PCR) using Taq DNA Polymerases (Thermo Fisher). Genomic DNA was purified from possible mutants and sequenced (Genewiz ...
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bioRxiv - Immunology 2020Quote: ... A pair of plasmids separately expressing the heavy- and light-chain of antibodies were transiently co-transfected into Expi293™ cells (Catalog No. A14528, ThermoFisher) with ExpiFectamine™ 293 Reagent ...
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bioRxiv - Cell Biology 2020Quote: ... Fifty nanograms of purified DNA was used for quantitative polymerase chain reaction (PCR) using TaqMan Genotyping Master Mix (Thermo Fisher Scientific) on an ABI7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2020Quote: ... This initial PEG treatment was followed by a secondary PEG treatment with 25mM short-chain 333 Da MS(PEG)4 Methyl-PEG-NHS-Ester Reagent (Thermo Scientific). These treatments resulted in a biotin-coated surface on the slide ...