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Citations for Thermo Fisher :
1951 - 2000 of 2521 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 58°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). For the telomere primers ...
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bioRxiv - Neuroscience 2024Quote: ... The reaction was cycled at 56°C annealing temperature along with a melt curve analysis using a QuantStudio 7 Flex (ThermoFisher). Each sample was run in duplicate ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s directions on a QuantStudio 7 Flex Real-Time PCR System (Cat. No. 4485701, Applied Biosystems, Waltham, MA). Amplification data and melt curves were analyzed using the QuantStudio Real-Time PCR software v1.7.1 ...
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bioRxiv - Immunology 2024Quote: ... or 96-well flat-bottom plates at a concentration of 7 × 105 – 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco). For both workflows ...
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bioRxiv - Neuroscience 2024Quote: ... was performed using the Applied Biosystems QuantStudio 7 Flex Real-Time PCR system and analysed with the QuantStudio Real-Time PCR Software (v1.7 Thermo Fisher). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... approximately 5 × 105 Huh-7 cells were transfected with 2 μg of purified BAC DNA using Lipofectamine 3,000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cancer Biology 2024Quote: ... the media was replaced with DMEM + 10% FBS + CellEvent Caspase-3/7 Detection Reagent (Green) according to the manufacturers recommendations (Invitrogen). The plate was then imaged every 15 minutes using phase and GFP channels in the ZEISS Celldiscoverer 7 Automated Live Cell Imager set to 42°C at 5% CO2 and 20% O2 ...
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bioRxiv - Cell Biology 2024Quote: ... 60L°C/60Lseconds), and melt curve (95L°C/15Lseconds, 60L°C/60Lseconds 95L°C/15Lseconds) on a QuantStudio 7 Flex (ThermoFisher). Messenger RNA (mRNA ...
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bioRxiv - Bioengineering 2024Quote: Reactive oxygen species (ROS) were quantitatively and qualitatively determined using the cell permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (ThermoFisher Scientific). H2DCFDA is a type of fluorescein that has been chemically reduced and is used to detect the generation of reactive oxygen intermediates in neutrophils and macrophages ...
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bioRxiv - Biochemistry 2024Quote: ... Liposomes at 5-7 mM were applied to the carbon side of a grid held in a Vitrobot Mark IV (ThermoFisher) chamber kept at 4°C and 100% relative humidity ...
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bioRxiv - Neuroscience 2021Quote: ... Gene expression was determined by qPCR using Brightgreen Dye qPCR master mix on a ViiA 7 Real Time PCR System (Applied Biosystems). Primer sequences were designed using NCBI Primer Blast ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Microbiology 2020Quote: ... The ORFs encoding the MV-C protein and Muc7 protein (Mucin 7 was used as a negative control) were cloned into entry vector pDON207 (Gateway system; Invitrogen, USA). They were transferred into the destination vector pSNL-N2 by Gateway cloning ...
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bioRxiv - Developmental Biology 2021Quote: ... Amplification and fluorescence detection of RT-qPCR were carried out on Applied Biosystems QuantStudio Flex 7 Real Time PCR System (ThermoFisher Scientific). The ΔΔCT method was used for relative quantification of target cDNA abundance representing mRNA expression level normalized to Rn18s (18S rRNA).
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bioRxiv - Cell Biology 2020Quote: ... cells (either MDA MB 231 GFP or a mixture of MDA MB 231 GFP and MCF 7 at a ratio of 1:1) were suspended in cold neutralized bovine collagen solution (2.5 mg/ml) (Thermo Fisher Scientific). This cell suspension was then added to pre-warmed (37 ̊C ...
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bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
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bioRxiv - Cell Biology 2020Quote: ... On day 7 the media was switched to repro media2 and E7 reprogramming media (1: 1) containing E6 basal media (Thermo Fisher) with NaB ...
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bioRxiv - Immunology 2021Quote: ... Quantitative real-time PCR was performed using PowerUp SYBR Green Gene Expression Assays (Thermofischer A25741) and a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). Relative expression values were calculated using the comparative Ct (ΔΔCt ...
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bioRxiv - Developmental Biology 2021Quote: ... qPCR was performed using SYBR Green PCR Master Mix (Lifetech, #43-676-59) on a ViiA 7 Real-Time PCR System (Applied Biosystems). The primers used are listed in Table S2 ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR reactions were performed from in vitro cultivated samples with 50ng +RT and –RT cDNA using a ViiA 7 Real-Time PCR system (Applied Biosystems) and Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified with Agencourt AMPure XP beads in 0.7:1 ratio (beads to PCR reaction volume) and quantified with the Quant-iT™ High-Sensitivity dsDNA Assay Kit (Invitrogen). The amplicons were pooled together and sequenced on the Illumina HiSeq 2500 using HiSeq Rapid SBS Kit v2 (500 cycles ...
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bioRxiv - Genomics 2020Quote: ... Experiments were performed in biological triplicates per condition and expression was measured in four technical replicates on QuantStudio 7 (Life Technologies) using TaqMan Gene Expression buffer (Life Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Axial and sagittal sections of the tissue at 7–10 μm thick were then processed and mounted onto cold Superfrost Plus slides (Fisher Scientific) by the PALM Histology Core Facility at the University of Ottawa ...
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bioRxiv - Developmental Biology 2022Quote: ... using KAPA SYBR FAST qPCR Master Mix (2X) ABI Prism (Thermo, KK4617) on the QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems) thermal cycler ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio 7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
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bioRxiv - Neuroscience 2021Quote: ... for 30 min followed by glycerol in PBS (volume ratio 7:3) for 30 min before being mounted onto superfrost slides (Fisher Scientific) for imaging ...
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bioRxiv - Cell Biology 2021Quote: ... Amplicons from the 7 clones that showed loss of a StyI site upon digestion were cloned into Zero Blunt TOPO (ThermoFisher K280020) and sequenced using primers SP6 and T7 ...
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bioRxiv - Molecular Biology 2021Quote: ... mouse anti-ELAV (MAb 7D, 1:20, which recognizes 7 amino acids unique to ELAV) and anti-GFP (1:250; Invitrogen A11122) and visualized with Alexa Fluor 488- and/or Alexa Fluor 647-coupled secondary antibodies (1:250 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR (qPCR) was performed using Power SybrGreen PCR master mix on a ViiA 7 real-time PCR system (Applied Biosystems). Expression of genes was normalised to three housekeeping genes (Gapdh ...
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bioRxiv - Molecular Biology 2021Quote: ... and real-time quantitative PCR was performed using a Viia 7 Real-Time PCR System on 384 custom-made TaqMan microfluidic cards (ThermoFisher, 4342253). CTs were normalised to the average CT values of Gapdh and Hprt ...
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bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures on feeder layers were performed on Mitomycin C-treated MEF as described above with passaging every 7 days using 1 mg/mL collagenase IV in DMEM/F12 (Life technologies) the feeder-free culture was performed on Geltrex™ (Life technologies ...
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bioRxiv - Immunology 2019Quote: ... Then to stain the cells we used antibodies against LFA-1 (clone I21/7) and 4G10 with Alexa Fluor 568–conjugated phalloidin (Life Technologies) and DAPI ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR reactions were performed using a Quant Studio 7 Flex Fast Real-Time PCR System (Applied Biosystems by life technologies) and the following Taqman primers (Thermo Fisher) ...
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bioRxiv - Genomics 2019Quote: ... Gene expression was determined via Taqman qPCR assays (Table S5) and run on the Applied Biosystems ViiATM 7 Real Time PCR system (ThermoFisher Scientific).
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bioRxiv - Systems Biology 2020Quote: ... and the pellet was resuspended in 7 mL PBS supplemented with 0.01 U/μL SUPERase In RNase Inhibitor (AM2696, Invitrogen, Carlsbad, CA), hereafter referred to as PBS-RI ...
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bioRxiv - Genetics 2019Quote: ... Quantitative real-time PCR analysis using the comparative Ct method was performed on the Applied Biosystem Vii 7 system (ThermoFisher Scientific) with the Powerup SYBR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... The valve then switches to engage the passive split such that a majority of LC effluent is diverted to waste prior to the third dimension PC. 13 RP-SAX-RP fractions were analyzed. Either an LTQ Velos (Figs. 1, 7) or Q-Exactive HF (Thermo Scientific) machine was used to acquire MS/MS spectra in data-dependent mode as described (Zhang et al. ...
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bioRxiv - Biophysics 2019Quote: The samples of 40-nm diameter dark red fluorescent beads deposited on a coverslip (Supplementary Fig. 6a, Supplementary Fig. 7) were obtained by diluting the initial solution (10720, Thermo Fisher) at 5.10−7 in PBS + 5% glucose ...
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bioRxiv - Physiology 2019Quote: Total RNA samples were extracted from one thousand 4-7 day-old Culex female antennae with TRIzol reagent (Invitrogen, Carlsbad, CA). Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... and 0,25 μM of forward and reverse primers (Table S5) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Genetics 2019Quote: ... The concentration of amplified cDNA was measured using the Qubit dsDNA High Sensitivity Assay Kit (Life 7 Technologies/Thermo Fisher Scientific). The cDNA size distribution of few selected wells was assessed on a High-Sensitivity Bioanalyzer Chip (Agilent) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quantitative PCR (QPCR) assays were performed in duplicate using the Applied Biosystems ViiA 7 System (Life Technologies, Grand Island, NY, USA). Housekeeping genes used to normalize the gene expression data were eliminated if associated with independent variables and covariates in analyses of variance (ANOVA) ...
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bioRxiv - Physiology 2019Quote: ... Quantitative RT-PCR was carried out with either TaqMan™ Universal PCR Master Mix or SYBR Green PCR master mix on the QuantStudio 7 Flex Real time PCR system (Applied Biosystems). All reactions were carried out in either duplicate or triplicate and Ct values were obtained ...
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bioRxiv - Bioengineering 2019Quote: Matrix remodeling associated gene expression profiles were examined for MSC-seeded hydrogel specimens via QuantiStudio 7 Flex real-time PCR (Applied Biosystems). Frozen hydrogels isolated at days 0 ...
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bioRxiv - Biochemistry 2021Quote: ... The grids were blotted for 6∼7 s at 100 % humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher). The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the following thermal cycling conditions ...
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bioRxiv - Bioengineering 2021Quote: ... coli Famp (ATCC #700891) suspension were added to 7 mL of top agar [0.5% (w/v) NaCl (Fisher Scientific #S271-500) and 0.7% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... cells were collected from popliteal lymph nodes 7 post immunization and surface stained (2×106 cells) with Fixable Blue Dead Cell Stain (Life Technologies), spike-AF647 (1 μg ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA samples were used as templates to perform real- time quantitative PCR with the power SYBR green mix (Abm, Cat#MasterMix-LR) on an Applied Biosystems QuantStudio (TM) 7 Flex System (Applied Biosystems). Duplicate runs of each sample were normalized to 36b4 to determine the relative transcript abundance of target genes ...