Labshake search
Citations for Thermo Fisher :
2001 - 2050 of 2521 citations for 7 Octenyldimethylmethoxysilane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2020Quote: ... from concentrated HTM cell culture supernatants ± DEX at 7 d supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) in 6X loading buffer (Boston Bio Products ...
-
bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies] ...
-
bioRxiv - Cancer Biology 2021Quote: ... H460 or MCF-7 cells with or without increasing numbers of MSCs were seeded in round-bottom 96-well plates (Nunclon Sphera, ThermoFisher Scientific). Living cell numbers and metabolic activity were measured with an MTT assay or labeled for 1 h using the LIVE/DEAD® kit (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: Stable transfections of OCI-LY-7 and Z-138 cells were performed by electroporation using the Neon® Transfection System (Invitrogen). Electroporation settings were selected after 24-well optimization was performed according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: Real-time PCR reactions were performed in triplicate in 96-well plates in a QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher). Equal initial amounts of total DNA were amplified in all conditions ...
-
S-nitrosoglutathione reductase deficiency causes aberrant placental S-nitrosylation and preeclampsiabioRxiv - Physiology 2021Quote: ... intracellular nitric oxide (NO) and peroxynitrite (ONOO‒) were measured by epifluorescence using 2’,7’-dichlorodihydrofluoresceine (H2DCF‒DA, 10 μM; Molecular Probes), 4,5-diaminofluorescein (DAF-2DA ...
-
bioRxiv - Cell Biology 2021Quote: ... A 10 μl aliquot of Spike S1 at 1 mg/ml as provided by the manufacturer was incubated for 20 min at r.t in the presence of a 10-fold molar excess of FITC or a 7-molar excess of Alexa Fluor 488 carboxylic acid succinimidyl ester (CASE) dye (ThermoFisher Scientific). Afterwards ...
-
bioRxiv - Plant Biology 2021Quote: ... insertions were additionally verified by flanking fragment sequencing (Supplementary Fig. 7) using the Phire Plant Direct PCR Kit (Thermo Fisher Scientific) and primers listed in Supplementary Table 1 ...
-
bioRxiv - Plant Biology 2020Quote: ... Detached roots were then incubated in 1X PBS (Phosphate Buffered Saline) buffer with 50 μM H2DCFDA (2’, 7’-dichlorodihydrofluorescein diacetate, Invitrogen, D399) for 30 min shaking at 200 rpm/min at room temperature (Allan and Fluhr ...
-
bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
-
bioRxiv - Systems Biology 2021Quote: ... mRNA expression of genes of interest was analyzed with TaqMan real-time PCR in a ViiA™ 7 system (Applied Biosystems). RNA sequencing library were prepared with Illumina RNA-Seq with Poly-A selections ...
-
bioRxiv - Plant Biology 2021Quote: ... and quantification PCRs were performed with ChamQ™ SYBR qPCR Master Mix (both from Vazyme Biotech co. ltd, Nanjing) on QuantStudio™ 7 Flex Real-Time PCR System (ThermoFisher). Three to four biological replicates from each of two to three independent trials were applied for each experiment ...
-
bioRxiv - Neuroscience 2020Quote: Adrenal glands and brain extracts were collected from P0-1 Syt-7 WT and KO mice and lysed in RIPA buffer supplemented with Protease Inhibitor Cocktail (Invitrogen, 89900). The supernatants were collected and protein concentrations were estimated by using the BCA Protein Assay Kit (Pierce 23227 ...
-
bioRxiv - Microbiology 2021Quote: ... from Mouse Antibody Isotyping 7-Plex ProcartaPlex were used as analytes and the samples were prepared according to manufacturer’s instructions (Thermo Fisher, UK).
-
bioRxiv - Microbiology 2020Quote: ... and quantitative real-time RT-PCR (qRT-PCR) was performed using the ViiA 7 real-time PCR detection platform (Applied BioSystems). Primers were based on previous studies (Ding et al. ...
-
bioRxiv - Cancer Biology 2020Quote: ... MCF-7 human breast carcinoma cells (ATCC, Rockville, MD) were grown in Dulbecco’s MEM (DMEM) with Glutamax-1 (Gibco, Paisley, Scotland) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Cancer Biology 2020Quote: Lungs and LNs were excised and immersed in 4% paraformaldehyde with 10% sucrose and 7% picric acid in PBS for 2 hours and then embedded in Tissue-Tec OCT (Fisher Scientific). 10 micron sections were prepared and slides were stained for B220 ...
-
bioRxiv - Cell Biology 2021Quote: ... qPCR was performed with the ViiA 7™ Real-Time PCR or ABI PRISM 7700 systems (Applied Biosystems, Foster City, CA). Methods are detailed in the online Supplementary material.
-
bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
-
bioRxiv - Biochemistry 2022Quote: ... and homogenized with 1xPBS containing protease and phosphatase inhibitors (Pierce) (7).Protein concentration was analyzed by standard BCA method with a NanoDrop spectrophotometer (ND-1000, Thermo Scientific). A 15% SDS-PAGE was used to run the samples at equal concentrations and transferred to a Immobilon-FL membrane ...
-
bioRxiv - Neuroscience 2022Quote: ... the percentage of apoptotic astrocytes was evaluated using CellEvent Caspase-3/7 Green Detection Reagent (1:250; Thermo Fisher, cat. #C10423) added directly to the medium ...
-
bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
-
bioRxiv - Biochemistry 2022Quote: ... Reactions were assembled in MicroAmp Optical 96-well reaction plates and carried out in a QuantStudio-7 Flex real-time PCR instrument (Applied Biosystems). PCR conditions were as follows ...
-
bioRxiv - Bioengineering 2022Quote: ... medium was changed to RPMI + B27-I and on day 7 to RPMI 1640 supplemented with B27 complete supplement (B27C; 17504044, Gibco, USA), which was thereafter used for CM culture ...
-
bioRxiv - Cancer Biology 2022Quote: siRNA oligos targeting human lnc152 [described previously (22)] were transfected into MCF-7 cells at a final concentration of 10 nM using Lipofectamine RNAiMAX reagent (Invitrogen, 13778150) according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2022Quote: ... Real-time PCR was performed on the Applied Biosystems ViiA 7 using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The level of ACTIN7 (ACT7 ...
-
bioRxiv - Bioengineering 2022Quote: ... 7 µm sections were cut on a Leica RM 2135 rotary microtome and mounted on Superfrost Plus microscope slides (Fisher Scientific).
-
bioRxiv - Biochemistry 2022Quote: ... at a concentration of 7 μM in the electroporation slurry (as previously described in (Alex et al., 2019)) (Neon Transfection System, Thermo Fisher). Control cells were electroporated with mCherry ...
-
bioRxiv - Immunology 2022Quote: 90μL of paraformaldehyde fixed cells was incubated with 5 x 10-7 M DAPI and 1:100 Vybrant™ DiD Cell-Labeling Solution (Invitrogen) at 37C for 20 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... 3.7 g/l sodium bicarbonate and sodium pyruvate) (SH30243.01, Hyclone GE) supplemented with 10% FBS (10270-106, Gibco-Thermo Fisher Scientific) and 1% penicillin-streptomycin (A001A ...
-
bioRxiv - Molecular Biology 2020Quote: ... The sonicated material was then pooled together and distributed into 500 μL aliquots (equivalent of 7*108 cells per aliquot) and 25 μl of Protein G magnetic beads (Life Technologies-Invitrogen) pre-bound with 6μg ...
-
bioRxiv - Immunology 2021Quote: ... for 35 minutes at 165V and transferred onto nitrocellulose membranes using the iBlot® 7-Minute Blotting System (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... total cellular RNA was extracted from Huh-7 cells treated with oleic acid for 24 hours using TRIZOL reagent (ThermoFisher Scientific) and reverse transcribed using the Superscript VILO (Life Technologies ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were then resuspended in DMEM + 10% FBS supplemented with 50 μM CellEvent™ Caspase 3/7 Green Detection Reagent (Invitrogen) at 37 °C for 25 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Real-time qPCR was performed using SYBR Green (BIO-RAD and a ViiA 7 Real Time System (ThermoFisher, Waltham, MA, USA) as specified by the manufacturer ...
-
bioRxiv - Microbiology 2021Quote: Huh-7 cells were transfected with the HBsAg or luciferase reporter derived plasmids per manufacturer’s instructions (Lipofectamine 3000, Invitrogen, MA, USA). Cells were treated with the indicated compounds for 5 days ...
-
bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed using an UltraSYBR Mixture (Beijing CoWin Biotech, Beijing, China) on a ViiA 7 real-time PCR system (Applied Biosystems). Viral rRNAs were normalized to swine 28S rRNA expression ...
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
-
bioRxiv - Neuroscience 2020Quote: ... PR1/2 and mPRs genes were determined through quantitative PCR (qPCR) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems), and data were analyzed with DataAssist (ABI ...
-
bioRxiv - Microbiology 2020Quote: Pseudo-typed viruses were produced as previously described (50), 293T (ATCC, CRL-3216), Huh-7 and Vero (ATCC, CCL-81) cells were maintained in high glucose DMEM (Gibco, USA) supplemented with 10% FBS (FBS ...
-
bioRxiv - Neuroscience 2021Quote: ... Neurospheres were passaged by centrifuging 5 min at 1,000 rpm and incubating for 7 min in Accutase (Gibco® A11105-01) before mechanically dissociating the spheres into single-cell suspension.
-
bioRxiv - Microbiology 2020Quote: ... di- and oligo- saccharides present in the spent media samples were analyzed on a Dionex ICS-5000 HPAEC system operated by the Chromeleon software version 7 (Dionex, Thermo Scientific). Samples were bound to a Dionex CarboPac PA1 (Thermo Scientific ...
-
bioRxiv - Biochemistry 2021Quote: ... Murine PNPLA2 mRNA levels relative to HPRT transcript levels were measured by the QuantStudio 7 Flex Real-Time PCR System using Taqman® gene expression assays (PNPLA2, Mm00503040_m1; HPRT, Mm00446968_m1, Thermo Fisher Scientific). PNPLA2 relative expression to HPRT was calculated using the comparative ΔΔCt method.27
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
-
bioRxiv - Neuroscience 2022Quote: For insulin and rapamycin treatment cells were seeded at 7 × 105 cells per well in a six-well plate (Thermo Scientific) in DMEM + 10% FBS ...
-
bioRxiv - Immunology 2022Quote: ... cDNA was amplified using the Fast SYBR Green Master Mix on a QuantStudio 7 Flex Real Time PCR Systems (Applied Biosystems). The sequences of primer pairs (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: About 80,000 MCF-7 cells were placed in each chamber of a 4-well chamber slide (Thermo Scientific, cat. no. 177399) containing 500 μl of Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Immunology 2022Quote: ... and Il-1β (in aortic CD3- cells) mRNA levels were assessed by qPCR using QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems), and initial differences in RNA quantity were corrected for using the housekeeping genes 36b4 and Cyclophilin B.
-
bioRxiv - Cancer Biology 2022Quote: ... Cell number per well by DNA content was determined after 7-10 days of culture using CyQUANT Direct Cell Proliferation Assay (Life Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2022Quote: ... PCR was performed for 55-60 cycles for maximal saturation of signal with final extension at 72 °C for 7 min in a 7500 Fast Real-Time PCR System (Applied Biosystems). The primer sequences used were ...