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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 8 4 chlorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Dry protein pellets of both ovary and hemolymph samples were dissolved and reduced in 8 M urea (Fisher Scientific, 45000234)/70 mM ammonium bicarbonate (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... GE Health Sciences) surface was conditioned by injecting 350 mM EDTA (diluted from 0.5 M, pH 8 stock, no. 15575020; Thermo Fisher) at 10 ul/min for 1 minute followed by extensive washing with running buffer to remove residual EDTA ...
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bioRxiv - Developmental Biology 2022Quote: ... the hPSC-PAX3 reporter line was used and the induction efficiency was reported along the 8 day experimental timeline using an inverted microscope (EVOS, Invitrogen). The PAX3 directional bias upon internal force generation was then evaluated in similar manner as the EdU ...
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bioRxiv - Biophysics 2022Quote: ... 2 µg fresh bacmid DNA was transfected into 2 mL Sf9 cells at 0.9×106 cells/2 mL with 8 µL Cellfection II (Thermo Fisher) following manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... 100,000 cells per well were seeded in Nunc™ Lab-Tek™ chambered coverglass 8 well plates (Thermo Fisher Scientific) 24 h prior to transfection ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... alkylated using iodoacetamide in 8M urea denaturation buffer (50mM Tris-HCl, pH 8) and digested overnight in 50mM ammonium bicarbonate using trypsin (ThermoFisher) at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were boiled for 5min at 95°C before being loaded on a 3-8% NuPAGE Tris-Acetate gel (Invitrogen). Western blots were quantified using ImageJ ...
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bioRxiv - Immunology 2021Quote: ... 1% PenStrep and 8 μg/mL puromycin (to ensure retention of TMPRSS2) with 5% CO2 in a 37°C incubator (ThermoFisher). Cells were trypsinized using 0.05% trypsin and plated to be at 90% confluence the following day ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Electroporated neurons were then transferred to pre-warmed neuronal media and plated at a density of 2,000 cells per well in an 8-well glass Lab-Tek slide (Thermo Scientific) pre-coated with poly-D-lysine (Corning ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were heated to 99 °C for 15 min and aliquots (5 µL) resolved by SDS-PAGE on 8 % – 16 % Tris-glycine minigels (Invitrogen) (150 V ...
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bioRxiv - Physiology 2021Quote: CMs isolated from WT and cMKO mice (8-10 weeks) labelled with NucBlue™ Live ReadyProbes™ Reagent (ThermoFisher Scientific) and Tetramethylrhodamine Ethyl Ester Perchlorate (TMRE ...
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bioRxiv - Neuroscience 2020Quote: To quantify oxidative stress/ ROS we used either 8 oxo-dG stainings or CellROX® assays (Thermo Fisher; Green Reagent). 8 oxo-dG (8-oxo-2’-deoxyguanosine ...
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bioRxiv - Microbiology 2021Quote: ... 1×104 transfected cells were seeded into each well of 8-well Lab-Tek™ II chamber slides (ThermoFisher Scientific) for immunostaining analysis ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were plated on poly-L-lysine hydrobromide-coated six-well culture plates for 8 days in Neurobasal Medium (GIBCO) supplemented with B27 ...
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bioRxiv - Neuroscience 2020Quote: ... total RNA was extracted from HNs after 8 days of tough decoy and shRNA treatment using TRIzol Reagent (Invitrogen, 15596026) in combination with RNeasy micro kit (Qiagen ...
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bioRxiv - Cancer Biology 2021Quote: ... carrying the mutation R273C was first amplified by PCR using the primers hp53-1 (5’-CACCATGGAGGAGCCGCAGTCAGATCC-3’) and hp53-8 (5’-GGATCCTCAGTCTGAGTCAGGCCCTTCTGTCTTG-3’) and cloned into the pENTR/D-TOPO vector (ThermoFisher) generating the entry vector pENTR p53(R273C ...
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bioRxiv - Systems Biology 2022Quote: ... The consistency of plasmid expression throughout different experiments was ensured by transfecting 80 ng of each plasmid per well of an 8-well Lab-Tek™ chambered cover glass slides (0.8 cm2 surface area, ThermoFisher) and the amount of plasmid was scaled up proportional to the culture-well surface area for other culture-plates used ...
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bioRxiv - Immunology 2022Quote: ... Cells were grown for six days at 37 °C with 8% CO2 shaking 125 rpm according to the manufacturer’s protocol (Thermo Fisher). Cell cultures for Spike-W and Spike Omicron BA.1 and BA.2 were harvested six days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... coated Corning dishes at 37°C in a 5% CO2 incubator and gently dissociated for passaging at 1:8 ratio every second day with Stem Pro Accutase (Gibco). Cells were washed with Dulbecco’s Phosphate Buffer Saline cell culture grade.
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bioRxiv - Molecular Biology 2022Quote: ... Cas9 protein was prepared by mixing 12 μl of Alt-R Cas9 protein (62 μM) with 8 μl of buffer R (Invitrogen). gRNA duplexes and Cas9 were then mixed gently and incubated at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2022Quote: Transduced BJ-hTERT cells were seeded in 6-well plates or 8-well chamber slides (Nunc™ Lab-Tek™). Cells were fixed in 4% paraformaldehyde in PBS (Alfa Aesar ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of samples (50-100 µg) were resolved by SDS-polyacrylamide gel electrophoresis in 3–8% Tris-Acetate gels (Thermofisher). Gels were wet-transferred in 1x NuPAGE Transfer Buffer (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... 8% Tris-glycine gels at 150 volts for 2.25 hr or pre-poured NuPAGE 3-8% Tris-Acetate gel (Invitrogen; EA03752) for 2.15 hr (Figure 1C ...
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bioRxiv - Cell Biology 2019Quote: ... dorsal root ganglion neurons were trimmed and isolated from postnatal 8-10 day SD rat spinal cords in HBSS (Invitrogen), and digested in 2.5 U/ml dispase II (Roche ...
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bioRxiv - Biophysics 2019Quote: QDs were functionalized with benzyl guanine by mixing 5 μl of 8 μM amino (PEG) QDs emitting at 655 nm (ThermoFisher) with 2 μl of 20 mM BG-GLA-NHS (NEB ...
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bioRxiv - Cell Biology 2019Quote: Cells expressing G12V Rac1A under a tetracycline-inducible promoter along with GFP-WASP or RFP-tagged active Rac marker were resuspended in LoFlo and seeded to a final concentration of 5×105 cells/ml on a borosilicate glass 8 well chamber (ThermoFisher) or on a 35 mm glass bottom dish (MatTek) ...
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bioRxiv - Genomics 2019Quote: ... hiPSCs were cultured on plates pre-coated with 0.5 μg/cm2 recombinant human Laminin-521 matrix (rhLaminin521; Biolamina) with daily changes of antibiotic-free Essential 8 (E8) media (ThermoFisher). Cells were passaged as small clumps with Versene (ThermoFisher) ...
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bioRxiv - Genetics 2019Quote: ... 10 or 15μg of protein was loaded per well on Tris-Acetate 3-8% gels with Tris-Acetate running buffer (NuPAGE, ThermoFisher) for detecting KIF26B protein or on 4-12% NuPAGE Bis-Tris Gel with NuPAGE MES SDS Running Buffer (NuPAGE ...
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bioRxiv - Cell Biology 2019Quote: MDA-MB-468 cells were grown to 70% confluence on 8-chambered glass slides (Nunc Lab-Tek II, Thermo Scientific), starved in serum-free media for 24 h,and were treated with DMSO carrier or SHP099 (10 uM ...
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bioRxiv - Cell Biology 2019Quote: MDA-MB-468 cells were grown to 70% confluence on 8-chambered glass slides (Nunc Lab-Tek II, Thermo Scientific), starved in serum-free media for 24 h,and were treated with DMSO carrier or SHP099 (10 uM ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and 64 °C) for 8 min using a VeriFlex temperature control technology of the thermocycler (Applied Biosystems VeritiTM Thermal Cycler). Immediately ...
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bioRxiv - Pathology 2020Quote: Stored blood samples collected on d 9 from selected 8 NEC and 8 healthy pigs across 3 litters were used for RNA extraction using MagMax 96 blood RNA isolation kit (Thermofisher) for transcriptomics by whole transcriptome shotgun sequencing as previously described24 ...
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bioRxiv - Developmental Biology 2020Quote: ... We analyzed 40 μg of protein by SDS-polyacrylamide gel electrophoresis on an 8% Tris-Glycine gel (Thermo Fisher Scientific) and transferred overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... The lines were grown and expanded in feeder-free conditions on vitronectin using Essential 8 plus medium (Thermo Fisher Scientific) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... On day 3 the cells were transfected with 60 pMol of either miR-122 or control microRNA (miR-122 p2-8) or anti-miR-122 LNA or control LNA (anti-miR-124) with 5uL of Lipofectamine 2000 (Invitrogen). Cells were harvested 2 days post-transfection and luciferase activity was measured.
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bioRxiv - Cancer Biology 2020Quote: ... The cell culture supernatant was collected and used for quantifying IL-8 secretion using the ELISA assay kit (# 88-8086-88, ThermoFisher). The assay was performed following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... 5×103 RAW 264.7 cells were seeded per well in a Nunc Lab-Tek II 8-well Chamber Slide system (ThermoFisher Scientific) in a total of 300 μl osteoclast differentiation medium containing 30 ng/ml RANKL ...
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bioRxiv - Microbiology 2021Quote: ... HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day or more prior to infection ...
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bioRxiv - Cancer Biology 2020Quote: ... 50μg of whole protein extracts from thymocytes and 50μl from myeloid cells were loaded on pre-cast NuPAGE™ BOLT 8% Bis-Tris polyacrylamide gels (Invitrogen) and transferred onto Immobilon-P PVDF membranes (Merck Millipore) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were transfected with 8μl (10 μM) small interfering RNA (sc-43983, Sant Cruz Biotechnology) with 8 μl Lipofectamine™ RNAiMAX (Invitrogen) for 6 hours according the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1×103 cells were seeded per well in either a 48-well or 8-chamber format in DMEM high glucose (Gibco) and refreshed every 3 to 4 days.
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bioRxiv - Biochemistry 2020Quote: ... the opsin-encoding constructs were fused in frame with a C-terminal 8-His tag and subcloned into the pPIC9K (A1ACR1, HfACR1 and AlACR2) or pPICZα (AlACR3) vector (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... The cells were transfected in triplicate with 10 μg of each bacmid DNA (bAcac13KO-ph, bAcac13REP-ph and bAc-ph)using 8 μL of Cellfectin II (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: HEK203-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day or more prior to infection ...
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bioRxiv - Immunology 2020Quote: HEK-hACE2 cells were cultured in DMEM with 10% FBS (Hyclone) and 1% PenStrep with 8% CO2 in a 37°C incubator (ThermoFisher). One day prior to infection ...
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bioRxiv - Plant Biology 2019Quote: ... as well as custom pENpAtPAD4 StrepII-YFP (Supplemental Dataset 8 with sequence in.gbk format) was performed using LR Clonase II (11791100, Life technologies).
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bioRxiv - Cell Biology 2021Quote: RBL-2H3 cells in a confluent 75 cm2 flask were washed and trypsinized for 8 min at 37 °C with 3 mL of 0.05% Trypsin-EDTA (Thermo Fisher). The detached cells were resuspended in 7 mL of growth medium and centrifuged to remove the medium ...